manipulation Search Results


m2 medium  (World Precision Instruments)
93
World Precision Instruments m2 medium
Following GVBD, Sgol2 Localizes on the Chromosome Arms and Kinetochores, and It Gradually Concentrates on Kinetochores during Late Metaphase (A) GV-stage oocytes harvested from Sgol2 -deleted females <t>were</t> <t>microinjected</t> in <t>M2</t> medium supplemented with IBMX with mRNA encoding GFP-Sgol2 and H2B-mCherry. After 1 hr of incubation, oocytes were released, and time-lapse confocal microscope images were captured for 12–14 hr following GVBD. Representative Z-projected images are displayed. (B) GV-stage oocytes harvested from wild-type control females were cultured for 2, 4, and 6 hr following GVBD. Chromosome spreads prepared at the indicated times following GVBD were stained for DNA (blue), Sgol2 (red), and CREST (green). See also <xref ref-type=Figure S2 . " width="250" height="auto" />
M2 Medium, supplied by World Precision Instruments, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/m2 medium/product/World Precision Instruments
Average 93 stars, based on 1 article reviews
m2 medium - by Bioz Stars, 2026-03
93/100 stars
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custom 4 axis motorized micromanipulator  (Siskiyou Corporation)
93
Siskiyou Corporation custom 4 axis motorized micromanipulator
Following GVBD, Sgol2 Localizes on the Chromosome Arms and Kinetochores, and It Gradually Concentrates on Kinetochores during Late Metaphase (A) GV-stage oocytes harvested from Sgol2 -deleted females <t>were</t> <t>microinjected</t> in <t>M2</t> medium supplemented with IBMX with mRNA encoding GFP-Sgol2 and H2B-mCherry. After 1 hr of incubation, oocytes were released, and time-lapse confocal microscope images were captured for 12–14 hr following GVBD. Representative Z-projected images are displayed. (B) GV-stage oocytes harvested from wild-type control females were cultured for 2, 4, and 6 hr following GVBD. Chromosome spreads prepared at the indicated times following GVBD were stained for DNA (blue), Sgol2 (red), and CREST (green). See also <xref ref-type=Figure S2 . " width="250" height="auto" />
Custom 4 Axis Motorized Micromanipulator, supplied by Siskiyou Corporation, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/custom 4 axis motorized micromanipulator/product/Siskiyou Corporation
Average 93 stars, based on 1 article reviews
custom 4 axis motorized micromanipulator - by Bioz Stars, 2026-03
93/100 stars
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manipulator  (Narishige inc)
97
Narishige inc manipulator
Following GVBD, Sgol2 Localizes on the Chromosome Arms and Kinetochores, and It Gradually Concentrates on Kinetochores during Late Metaphase (A) GV-stage oocytes harvested from Sgol2 -deleted females <t>were</t> <t>microinjected</t> in <t>M2</t> medium supplemented with IBMX with mRNA encoding GFP-Sgol2 and H2B-mCherry. After 1 hr of incubation, oocytes were released, and time-lapse confocal microscope images were captured for 12–14 hr following GVBD. Representative Z-projected images are displayed. (B) GV-stage oocytes harvested from wild-type control females were cultured for 2, 4, and 6 hr following GVBD. Chromosome spreads prepared at the indicated times following GVBD were stained for DNA (blue), Sgol2 (red), and CREST (green). See also <xref ref-type=Figure S2 . " width="250" height="auto" />
Manipulator, supplied by Narishige inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/manipulator/product/Narishige inc
Average 97 stars, based on 1 article reviews
manipulator - by Bioz Stars, 2026-03
97/100 stars
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narishige 3 axis oil hydraulic micromanipulator  (Narishige inc)
93
Narishige inc narishige 3 axis oil hydraulic micromanipulator
Following GVBD, Sgol2 Localizes on the Chromosome Arms and Kinetochores, and It Gradually Concentrates on Kinetochores during Late Metaphase (A) GV-stage oocytes harvested from Sgol2 -deleted females <t>were</t> <t>microinjected</t> in <t>M2</t> medium supplemented with IBMX with mRNA encoding GFP-Sgol2 and H2B-mCherry. After 1 hr of incubation, oocytes were released, and time-lapse confocal microscope images were captured for 12–14 hr following GVBD. Representative Z-projected images are displayed. (B) GV-stage oocytes harvested from wild-type control females were cultured for 2, 4, and 6 hr following GVBD. Chromosome spreads prepared at the indicated times following GVBD were stained for DNA (blue), Sgol2 (red), and CREST (green). See also <xref ref-type=Figure S2 . " width="250" height="auto" />
Narishige 3 Axis Oil Hydraulic Micromanipulator, supplied by Narishige inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/narishige 3 axis oil hydraulic micromanipulator/product/Narishige inc
Average 93 stars, based on 1 article reviews
narishige 3 axis oil hydraulic micromanipulator - by Bioz Stars, 2026-03
93/100 stars
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injectman n12 manipulator  (Eppendorf AG)
99
Eppendorf AG injectman n12 manipulator
Following GVBD, Sgol2 Localizes on the Chromosome Arms and Kinetochores, and It Gradually Concentrates on Kinetochores during Late Metaphase (A) GV-stage oocytes harvested from Sgol2 -deleted females <t>were</t> <t>microinjected</t> in <t>M2</t> medium supplemented with IBMX with mRNA encoding GFP-Sgol2 and H2B-mCherry. After 1 hr of incubation, oocytes were released, and time-lapse confocal microscope images were captured for 12–14 hr following GVBD. Representative Z-projected images are displayed. (B) GV-stage oocytes harvested from wild-type control females were cultured for 2, 4, and 6 hr following GVBD. Chromosome spreads prepared at the indicated times following GVBD were stained for DNA (blue), Sgol2 (red), and CREST (green). See also <xref ref-type=Figure S2 . " width="250" height="auto" />
Injectman N12 Manipulator, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/injectman n12 manipulator/product/Eppendorf AG
Average 99 stars, based on 1 article reviews
injectman n12 manipulator - by Bioz Stars, 2026-03
99/100 stars
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remote hydraulic micromanipulator  (Narishige inc)
91
Narishige inc remote hydraulic micromanipulator
Following GVBD, Sgol2 Localizes on the Chromosome Arms and Kinetochores, and It Gradually Concentrates on Kinetochores during Late Metaphase (A) GV-stage oocytes harvested from Sgol2 -deleted females <t>were</t> <t>microinjected</t> in <t>M2</t> medium supplemented with IBMX with mRNA encoding GFP-Sgol2 and H2B-mCherry. After 1 hr of incubation, oocytes were released, and time-lapse confocal microscope images were captured for 12–14 hr following GVBD. Representative Z-projected images are displayed. (B) GV-stage oocytes harvested from wild-type control females were cultured for 2, 4, and 6 hr following GVBD. Chromosome spreads prepared at the indicated times following GVBD were stained for DNA (blue), Sgol2 (red), and CREST (green). See also <xref ref-type=Figure S2 . " width="250" height="auto" />
Remote Hydraulic Micromanipulator, supplied by Narishige inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/remote hydraulic micromanipulator/product/Narishige inc
Average 91 stars, based on 1 article reviews
remote hydraulic micromanipulator - by Bioz Stars, 2026-03
91/100 stars
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narishige manipulators  (Narishige inc)
94
Narishige inc narishige manipulators
Following GVBD, Sgol2 Localizes on the Chromosome Arms and Kinetochores, and It Gradually Concentrates on Kinetochores during Late Metaphase (A) GV-stage oocytes harvested from Sgol2 -deleted females <t>were</t> <t>microinjected</t> in <t>M2</t> medium supplemented with IBMX with mRNA encoding GFP-Sgol2 and H2B-mCherry. After 1 hr of incubation, oocytes were released, and time-lapse confocal microscope images were captured for 12–14 hr following GVBD. Representative Z-projected images are displayed. (B) GV-stage oocytes harvested from wild-type control females were cultured for 2, 4, and 6 hr following GVBD. Chromosome spreads prepared at the indicated times following GVBD were stained for DNA (blue), Sgol2 (red), and CREST (green). See also <xref ref-type=Figure S2 . " width="250" height="auto" />
Narishige Manipulators, supplied by Narishige inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/narishige manipulators/product/Narishige inc
Average 94 stars, based on 1 article reviews
narishige manipulators - by Bioz Stars, 2026-03
94/100 stars
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manipulator  (Eppendorf AG)
99
Eppendorf AG manipulator
Following GVBD, Sgol2 Localizes on the Chromosome Arms and Kinetochores, and It Gradually Concentrates on Kinetochores during Late Metaphase (A) GV-stage oocytes harvested from Sgol2 -deleted females <t>were</t> <t>microinjected</t> in <t>M2</t> medium supplemented with IBMX with mRNA encoding GFP-Sgol2 and H2B-mCherry. After 1 hr of incubation, oocytes were released, and time-lapse confocal microscope images were captured for 12–14 hr following GVBD. Representative Z-projected images are displayed. (B) GV-stage oocytes harvested from wild-type control females were cultured for 2, 4, and 6 hr following GVBD. Chromosome spreads prepared at the indicated times following GVBD were stained for DNA (blue), Sgol2 (red), and CREST (green). See also <xref ref-type=Figure S2 . " width="250" height="auto" />
Manipulator, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/manipulator/product/Eppendorf AG
Average 99 stars, based on 1 article reviews
manipulator - by Bioz Stars, 2026-03
99/100 stars
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motor driven micromanipulator  (World Precision Instruments)
90
World Precision Instruments motor driven micromanipulator
Following GVBD, Sgol2 Localizes on the Chromosome Arms and Kinetochores, and It Gradually Concentrates on Kinetochores during Late Metaphase (A) GV-stage oocytes harvested from Sgol2 -deleted females <t>were</t> <t>microinjected</t> in <t>M2</t> medium supplemented with IBMX with mRNA encoding GFP-Sgol2 and H2B-mCherry. After 1 hr of incubation, oocytes were released, and time-lapse confocal microscope images were captured for 12–14 hr following GVBD. Representative Z-projected images are displayed. (B) GV-stage oocytes harvested from wild-type control females were cultured for 2, 4, and 6 hr following GVBD. Chromosome spreads prepared at the indicated times following GVBD were stained for DNA (blue), Sgol2 (red), and CREST (green). See also <xref ref-type=Figure S2 . " width="250" height="auto" />
Motor Driven Micromanipulator, supplied by World Precision Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/motor driven micromanipulator/product/World Precision Instruments
Average 90 stars, based on 1 article reviews
motor driven micromanipulator - by Bioz Stars, 2026-03
90/100 stars
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microinjection manipulator  (Eppendorf AG)
96
Eppendorf AG microinjection manipulator
Following GVBD, Sgol2 Localizes on the Chromosome Arms and Kinetochores, and It Gradually Concentrates on Kinetochores during Late Metaphase (A) GV-stage oocytes harvested from Sgol2 -deleted females <t>were</t> <t>microinjected</t> in <t>M2</t> medium supplemented with IBMX with mRNA encoding GFP-Sgol2 and H2B-mCherry. After 1 hr of incubation, oocytes were released, and time-lapse confocal microscope images were captured for 12–14 hr following GVBD. Representative Z-projected images are displayed. (B) GV-stage oocytes harvested from wild-type control females were cultured for 2, 4, and 6 hr following GVBD. Chromosome spreads prepared at the indicated times following GVBD were stained for DNA (blue), Sgol2 (red), and CREST (green). See also <xref ref-type=Figure S2 . " width="250" height="auto" />
Microinjection Manipulator, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/microinjection manipulator/product/Eppendorf AG
Average 96 stars, based on 1 article reviews
microinjection manipulator - by Bioz Stars, 2026-03
96/100 stars
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m 5 azacytidine  (World Precision Instruments)
86
World Precision Instruments m 5 azacytidine
Following GVBD, Sgol2 Localizes on the Chromosome Arms and Kinetochores, and It Gradually Concentrates on Kinetochores during Late Metaphase (A) GV-stage oocytes harvested from Sgol2 -deleted females <t>were</t> <t>microinjected</t> in <t>M2</t> medium supplemented with IBMX with mRNA encoding GFP-Sgol2 and H2B-mCherry. After 1 hr of incubation, oocytes were released, and time-lapse confocal microscope images were captured for 12–14 hr following GVBD. Representative Z-projected images are displayed. (B) GV-stage oocytes harvested from wild-type control females were cultured for 2, 4, and 6 hr following GVBD. Chromosome spreads prepared at the indicated times following GVBD were stained for DNA (blue), Sgol2 (red), and CREST (green). See also <xref ref-type=Figure S2 . " width="250" height="auto" />
M 5 Azacytidine, supplied by World Precision Instruments, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/m 5 azacytidine/product/World Precision Instruments
Average 86 stars, based on 1 article reviews
m 5 azacytidine - by Bioz Stars, 2026-03
86/100 stars
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um 3c coarse manipulator  (Narishige inc)
91
Narishige inc um 3c coarse manipulator
Following GVBD, Sgol2 Localizes on the Chromosome Arms and Kinetochores, and It Gradually Concentrates on Kinetochores during Late Metaphase (A) GV-stage oocytes harvested from Sgol2 -deleted females <t>were</t> <t>microinjected</t> in <t>M2</t> medium supplemented with IBMX with mRNA encoding GFP-Sgol2 and H2B-mCherry. After 1 hr of incubation, oocytes were released, and time-lapse confocal microscope images were captured for 12–14 hr following GVBD. Representative Z-projected images are displayed. (B) GV-stage oocytes harvested from wild-type control females were cultured for 2, 4, and 6 hr following GVBD. Chromosome spreads prepared at the indicated times following GVBD were stained for DNA (blue), Sgol2 (red), and CREST (green). See also <xref ref-type=Figure S2 . " width="250" height="auto" />
Um 3c Coarse Manipulator, supplied by Narishige inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/um 3c coarse manipulator/product/Narishige inc
Average 91 stars, based on 1 article reviews
um 3c coarse manipulator - by Bioz Stars, 2026-03
91/100 stars
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Image Search Results


Following GVBD, Sgol2 Localizes on the Chromosome Arms and Kinetochores, and It Gradually Concentrates on Kinetochores during Late Metaphase (A) GV-stage oocytes harvested from Sgol2 -deleted females were microinjected in M2 medium supplemented with IBMX with mRNA encoding GFP-Sgol2 and H2B-mCherry. After 1 hr of incubation, oocytes were released, and time-lapse confocal microscope images were captured for 12–14 hr following GVBD. Representative Z-projected images are displayed. (B) GV-stage oocytes harvested from wild-type control females were cultured for 2, 4, and 6 hr following GVBD. Chromosome spreads prepared at the indicated times following GVBD were stained for DNA (blue), Sgol2 (red), and CREST (green). See also <xref ref-type=Figure S2 . " width="100%" height="100%">

Journal: Current Biology

Article Title: APC/C Cdh1 Enables Removal of Shugoshin-2 from the Arms of Bivalent Chromosomes by Moderating Cyclin-Dependent Kinase Activity

doi: 10.1016/j.cub.2017.04.023

Figure Lengend Snippet: Following GVBD, Sgol2 Localizes on the Chromosome Arms and Kinetochores, and It Gradually Concentrates on Kinetochores during Late Metaphase (A) GV-stage oocytes harvested from Sgol2 -deleted females were microinjected in M2 medium supplemented with IBMX with mRNA encoding GFP-Sgol2 and H2B-mCherry. After 1 hr of incubation, oocytes were released, and time-lapse confocal microscope images were captured for 12–14 hr following GVBD. Representative Z-projected images are displayed. (B) GV-stage oocytes harvested from wild-type control females were cultured for 2, 4, and 6 hr following GVBD. Chromosome spreads prepared at the indicated times following GVBD were stained for DNA (blue), Sgol2 (red), and CREST (green). See also Figure S2 .

Article Snippet: About 5-10 pL of in-vitro transcribed mRNA at 0.1 mg/mL in RNase-free water (Ambion) was microinjected into mature oocytes in M2 medium (under oil) using a Pneumatic PicoPump (World Precision Instruments).

Techniques: Incubation, Microscopy, Cell Culture, Staining

Journal: Current Biology

Article Title: APC/C Cdh1 Enables Removal of Shugoshin-2 from the Arms of Bivalent Chromosomes by Moderating Cyclin-Dependent Kinase Activity

doi: 10.1016/j.cub.2017.04.023

Figure Lengend Snippet:

Article Snippet: About 5-10 pL of in-vitro transcribed mRNA at 0.1 mg/mL in RNase-free water (Ambion) was microinjected into mature oocytes in M2 medium (under oil) using a Pneumatic PicoPump (World Precision Instruments).

Techniques: Purification, Recombinant, Molecular Weight, Western Blot, Membrane, Software, Suspension