Journal: British Journal of Cancer

Article Title: Proteomic and metabonomic biomarkers for hepatocellular carcinoma: a comprehensive review

doi: 10.1038/bjc.2015.38

Figure Lengend Snippet: Summary of proteomic studies that were reviewed

Article Snippet: , , Tissue , ↑ HCC:CON , 100% of HCCs , 6 HCC, 6 non-malignant adjacent tissue , HBV , — , 2.1 † , 2DE-coupled MALDI–TOF–MS , Matos et al , 2009 .

Techniques: Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Protease Inhibitor, Biomarker Discovery

Journal: Chemistry (Weinheim an Der Bergstrasse, Germany)

Article Title: Synthesis of 5‐Alkyl‐ and 5‐Phenylamino‐Substituted Azothiazole Dyes with Solvatochromic and DNA‐Binding Properties

doi: 10.1002/chem.201903657

Figure Lengend Snippet: Spectrophotometric titration of 7 b (1) and 8 b (2) with ct DNA [A, c L =10 μ m , c DNA =2.17 m m (A1), c DNA =1.49 m m (A2); c DNA in base pairs] in BPE buffer ( c Na+ =16 m m , pH 7.0; with 5 % v/v DMSO), 22AG (B, c L =5 μ m , c 22AG =285 μ m ; c 22AG in oligonucleotide) in K‐phosphate buffer pH 7.0 ( c K+ =73 m m ; with 5 % v/v DMSO) and PSS [C, c L =10 μ m , c PSS =190 μ m (C1), c PSS =195 μ m (C2)] in BPE buffer ( c Na+ =16 m m , pH 7.0; with 2.5–5 % v/v DMSO). Red: Spectra of the pure ligand solutions; blue: spectra at the end of the titrations. The arrows indicate the changes of absorption upon addition of the host molecule. Insets: Plot of Abs./ Abs. 0 versus c DNA / c L or c PSS / c L .

Article Snippet: The oligodeoxyribonucleotide 22AG d[A(GGGTTA) 3 GGG] (purification: HPLC; quality control: MALDI‐TOF; synthesis scale: 1.0 μmol) was purchased from biomers.net GmbH (Ulm, Germany).

Techniques: Titration

Journal: Chemistry (Weinheim an Der Bergstrasse, Germany)

Article Title: Synthesis of 5‐Alkyl‐ and 5‐Phenylamino‐Substituted Azothiazole Dyes with Solvatochromic and DNA‐Binding Properties

doi: 10.1002/chem.201903657

Figure Lengend Snippet: Absorption and Emission Properties of 7 b and 8 b in the Presence of ct DNA, 22AG and PSS.

Article Snippet: The oligodeoxyribonucleotide 22AG d[A(GGGTTA) 3 GGG] (purification: HPLC; quality control: MALDI‐TOF; synthesis scale: 1.0 μmol) was purchased from biomers.net GmbH (Ulm, Germany).

Techniques:

Journal: Chemistry (Weinheim an Der Bergstrasse, Germany)

Article Title: Synthesis of 5‐Alkyl‐ and 5‐Phenylamino‐Substituted Azothiazole Dyes with Solvatochromic and DNA‐Binding Properties

doi: 10.1002/chem.201903657

Figure Lengend Snippet: Spectrofluorimetric titration of 8 b with ct DNA (A, c L =10 μ m , c DNA =1.49 m m ; c DNA in base pairs) in BPE buffer ( c Na+ =16 m m , pH 7.0; with 5 % v/v DMSO), 22AG (B, c L =5 μ m , c 22AG =285 μ m ; c 22AG in oligonucleotide) in K‐phosphate buffer ( c K+ =73 m m , pH 7.0; with 5 % v/v DMSO) and PSS (C, c L =10 μ m , c PSS =195 μ m ) in BPE buffer ( c Na+ =16 m m , pH 7.0; with 2.5 % v/v DMSO); λ ex =515 nm. Red: Spectra of the pure ligand solutions; blue: spectra at the end of the titrations. The arrows indicate the changes in emission intensity upon addition of the host molecule. Insets: Plot of the relative fluorescence intensity I / I 0 (corrected with regard to the change of the absorption at the excitation wavelength) versus c DNA / c L or c PSS / c L . Inset pictures in A: Fluorescence colors of 8 b in the absence and in the presence of ct DNA; λ ex =366 nm. The contrast and brightness were enhanced by 30 % without changing the true colors (cf. Figure S5).

Article Snippet: The oligodeoxyribonucleotide 22AG d[A(GGGTTA) 3 GGG] (purification: HPLC; quality control: MALDI‐TOF; synthesis scale: 1.0 μmol) was purchased from biomers.net GmbH (Ulm, Germany).

Techniques: Titration, Fluorescence