mafa Search Results


gene exp klrg1 hs00195153 m1  (Thermo Fisher)
86
Thermo Fisher gene exp klrg1 hs00195153 m1
Gene Exp Klrg1 Hs00195153 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
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apc  (Miltenyi Biotec)
93
Miltenyi Biotec apc
Apc, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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93/100 stars
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rabbit antibody to mafa  (Bethyl)
93
Bethyl rabbit antibody to mafa
Figure 3 <t>MAFA</t> as a target of miR-204. (a,b) The effects of miR-204 on Mafa mRNA (a) and protein (b) expression as assessed by qRT-PCR and immunoblotting. (c) Changes in the Mafa occupancy of the insulin promoter in response to miR-204 as measured by <t>ChIP.</t> <t>IgG</t> control results are shown on the right. Gapdh, glyceraldehyde 3-phosphate dehydrogenase. (d) Alignment of the miR-204 seed sequence (arrow) and the wild-type (WT) rat Mafa 3′ UTR target sequence (bold) and the mutated (M) target sequence (gray). (e) miR-204–directed repression of the luciferase reporter gene bearing the WT or mutant Mafa 3′ UTR segments as assessed 24 h after cotransfecting INS-1 cells with the wild-type or mutant reporter plasmids. All data are shown as the mean ± s.e.m. of three independent experiments, and one representative immunoblot is shown. *P < 0.05, **P < 0.01 (Student’s t test). NS, not significant.
Rabbit Antibody To Mafa, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit antibody to mafa/product/Bethyl
Average 93 stars, based on 1 article reviews
rabbit antibody to mafa - by Bioz Stars, 2026-03
93/100 stars
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rabbit anti mafa  (Bethyl)
93
Bethyl rabbit anti mafa
Figure 3 <t>MAFA</t> as a target of miR-204. (a,b) The effects of miR-204 on Mafa mRNA (a) and protein (b) expression as assessed by qRT-PCR and immunoblotting. (c) Changes in the Mafa occupancy of the insulin promoter in response to miR-204 as measured by <t>ChIP.</t> <t>IgG</t> control results are shown on the right. Gapdh, glyceraldehyde 3-phosphate dehydrogenase. (d) Alignment of the miR-204 seed sequence (arrow) and the wild-type (WT) rat Mafa 3′ UTR target sequence (bold) and the mutated (M) target sequence (gray). (e) miR-204–directed repression of the luciferase reporter gene bearing the WT or mutant Mafa 3′ UTR segments as assessed 24 h after cotransfecting INS-1 cells with the wild-type or mutant reporter plasmids. All data are shown as the mean ± s.e.m. of three independent experiments, and one representative immunoblot is shown. *P < 0.05, **P < 0.01 (Student’s t test). NS, not significant.
Rabbit Anti Mafa, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti mafa/product/Bethyl
Average 93 stars, based on 1 article reviews
rabbit anti mafa - by Bioz Stars, 2026-03
93/100 stars
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mafa  (OriGene)
94
OriGene mafa
Figure 3 <t>MAFA</t> as a target of miR-204. (a,b) The effects of miR-204 on Mafa mRNA (a) and protein (b) expression as assessed by qRT-PCR and immunoblotting. (c) Changes in the Mafa occupancy of the insulin promoter in response to miR-204 as measured by <t>ChIP.</t> <t>IgG</t> control results are shown on the right. Gapdh, glyceraldehyde 3-phosphate dehydrogenase. (d) Alignment of the miR-204 seed sequence (arrow) and the wild-type (WT) rat Mafa 3′ UTR target sequence (bold) and the mutated (M) target sequence (gray). (e) miR-204–directed repression of the luciferase reporter gene bearing the WT or mutant Mafa 3′ UTR segments as assessed 24 h after cotransfecting INS-1 cells with the wild-type or mutant reporter plasmids. All data are shown as the mean ± s.e.m. of three independent experiments, and one representative immunoblot is shown. *P < 0.05, **P < 0.01 (Student’s t test). NS, not significant.
Mafa, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mafa/product/OriGene
Average 94 stars, based on 1 article reviews
mafa - by Bioz Stars, 2026-03
94/100 stars
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mouse mafa cst  (Proteintech)
91
Proteintech mouse mafa cst
Figure 3 <t>MAFA</t> as a target of miR-204. (a,b) The effects of miR-204 on Mafa mRNA (a) and protein (b) expression as assessed by qRT-PCR and immunoblotting. (c) Changes in the Mafa occupancy of the insulin promoter in response to miR-204 as measured by <t>ChIP.</t> <t>IgG</t> control results are shown on the right. Gapdh, glyceraldehyde 3-phosphate dehydrogenase. (d) Alignment of the miR-204 seed sequence (arrow) and the wild-type (WT) rat Mafa 3′ UTR target sequence (bold) and the mutated (M) target sequence (gray). (e) miR-204–directed repression of the luciferase reporter gene bearing the WT or mutant Mafa 3′ UTR segments as assessed 24 h after cotransfecting INS-1 cells with the wild-type or mutant reporter plasmids. All data are shown as the mean ± s.e.m. of three independent experiments, and one representative immunoblot is shown. *P < 0.05, **P < 0.01 (Student’s t test). NS, not significant.
Mouse Mafa Cst, supplied by Proteintech, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse mafa cst/product/Proteintech
Average 91 stars, based on 1 article reviews
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pe cy7 anti klrg1  (Miltenyi Biotec)
94
Miltenyi Biotec pe cy7 anti klrg1
Figure 3 <t>MAFA</t> as a target of miR-204. (a,b) The effects of miR-204 on Mafa mRNA (a) and protein (b) expression as assessed by qRT-PCR and immunoblotting. (c) Changes in the Mafa occupancy of the insulin promoter in response to miR-204 as measured by <t>ChIP.</t> <t>IgG</t> control results are shown on the right. Gapdh, glyceraldehyde 3-phosphate dehydrogenase. (d) Alignment of the miR-204 seed sequence (arrow) and the wild-type (WT) rat Mafa 3′ UTR target sequence (bold) and the mutated (M) target sequence (gray). (e) miR-204–directed repression of the luciferase reporter gene bearing the WT or mutant Mafa 3′ UTR segments as assessed 24 h after cotransfecting INS-1 cells with the wild-type or mutant reporter plasmids. All data are shown as the mean ± s.e.m. of three independent experiments, and one representative immunoblot is shown. *P < 0.05, **P < 0.01 (Student’s t test). NS, not significant.
Pe Cy7 Anti Klrg1, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
pe cy7 anti klrg1 - by Bioz Stars, 2026-03
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gene exp mafa hs01651425 s1  (Thermo Fisher)
97
Thermo Fisher gene exp mafa hs01651425 s1
Figure 3 <t>MAFA</t> as a target of miR-204. (a,b) The effects of miR-204 on Mafa mRNA (a) and protein (b) expression as assessed by qRT-PCR and immunoblotting. (c) Changes in the Mafa occupancy of the insulin promoter in response to miR-204 as measured by <t>ChIP.</t> <t>IgG</t> control results are shown on the right. Gapdh, glyceraldehyde 3-phosphate dehydrogenase. (d) Alignment of the miR-204 seed sequence (arrow) and the wild-type (WT) rat Mafa 3′ UTR target sequence (bold) and the mutated (M) target sequence (gray). (e) miR-204–directed repression of the luciferase reporter gene bearing the WT or mutant Mafa 3′ UTR segments as assessed 24 h after cotransfecting INS-1 cells with the wild-type or mutant reporter plasmids. All data are shown as the mean ± s.e.m. of three independent experiments, and one representative immunoblot is shown. *P < 0.05, **P < 0.01 (Student’s t test). NS, not significant.
Gene Exp Mafa Hs01651425 S1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 97 stars, based on 1 article reviews
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97/100 stars
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antimafa polyclonal antibody  (Novus Biologicals)
94
Novus Biologicals antimafa polyclonal antibody
Figure 3 <t>MAFA</t> as a target of miR-204. (a,b) The effects of miR-204 on Mafa mRNA (a) and protein (b) expression as assessed by qRT-PCR and immunoblotting. (c) Changes in the Mafa occupancy of the insulin promoter in response to miR-204 as measured by <t>ChIP.</t> <t>IgG</t> control results are shown on the right. Gapdh, glyceraldehyde 3-phosphate dehydrogenase. (d) Alignment of the miR-204 seed sequence (arrow) and the wild-type (WT) rat Mafa 3′ UTR target sequence (bold) and the mutated (M) target sequence (gray). (e) miR-204–directed repression of the luciferase reporter gene bearing the WT or mutant Mafa 3′ UTR segments as assessed 24 h after cotransfecting INS-1 cells with the wild-type or mutant reporter plasmids. All data are shown as the mean ± s.e.m. of three independent experiments, and one representative immunoblot is shown. *P < 0.05, **P < 0.01 (Student’s t test). NS, not significant.
Antimafa Polyclonal Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antimafa polyclonal antibody/product/Novus Biologicals
Average 94 stars, based on 1 article reviews
antimafa polyclonal antibody - by Bioz Stars, 2026-03
94/100 stars
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gene exp mafa hs04419852 s1  (Thermo Fisher)
94
Thermo Fisher gene exp mafa hs04419852 s1
Figure 3 <t>MAFA</t> as a target of miR-204. (a,b) The effects of miR-204 on Mafa mRNA (a) and protein (b) expression as assessed by qRT-PCR and immunoblotting. (c) Changes in the Mafa occupancy of the insulin promoter in response to miR-204 as measured by <t>ChIP.</t> <t>IgG</t> control results are shown on the right. Gapdh, glyceraldehyde 3-phosphate dehydrogenase. (d) Alignment of the miR-204 seed sequence (arrow) and the wild-type (WT) rat Mafa 3′ UTR target sequence (bold) and the mutated (M) target sequence (gray). (e) miR-204–directed repression of the luciferase reporter gene bearing the WT or mutant Mafa 3′ UTR segments as assessed 24 h after cotransfecting INS-1 cells with the wild-type or mutant reporter plasmids. All data are shown as the mean ± s.e.m. of three independent experiments, and one representative immunoblot is shown. *P < 0.05, **P < 0.01 (Student’s t test). NS, not significant.
Gene Exp Mafa Hs04419852 S1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gene exp mafa hs04419852 s1/product/Thermo Fisher
Average 94 stars, based on 1 article reviews
gene exp mafa hs04419852 s1 - by Bioz Stars, 2026-03
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gene exp mafa mm00845206 s1  (Thermo Fisher)
88
Thermo Fisher gene exp mafa mm00845206 s1
Figure 3 <t>MAFA</t> as a target of miR-204. (a,b) The effects of miR-204 on Mafa mRNA (a) and protein (b) expression as assessed by qRT-PCR and immunoblotting. (c) Changes in the Mafa occupancy of the insulin promoter in response to miR-204 as measured by <t>ChIP.</t> <t>IgG</t> control results are shown on the right. Gapdh, glyceraldehyde 3-phosphate dehydrogenase. (d) Alignment of the miR-204 seed sequence (arrow) and the wild-type (WT) rat Mafa 3′ UTR target sequence (bold) and the mutated (M) target sequence (gray). (e) miR-204–directed repression of the luciferase reporter gene bearing the WT or mutant Mafa 3′ UTR segments as assessed 24 h after cotransfecting INS-1 cells with the wild-type or mutant reporter plasmids. All data are shown as the mean ± s.e.m. of three independent experiments, and one representative immunoblot is shown. *P < 0.05, **P < 0.01 (Student’s t test). NS, not significant.
Gene Exp Mafa Mm00845206 S1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gene exp mafa mm00845206 s1/product/Thermo Fisher
Average 88 stars, based on 1 article reviews
gene exp mafa mm00845206 s1 - by Bioz Stars, 2026-03
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Image Search Results


Figure 3 MAFA as a target of miR-204. (a,b) The effects of miR-204 on Mafa mRNA (a) and protein (b) expression as assessed by qRT-PCR and immunoblotting. (c) Changes in the Mafa occupancy of the insulin promoter in response to miR-204 as measured by ChIP. IgG control results are shown on the right. Gapdh, glyceraldehyde 3-phosphate dehydrogenase. (d) Alignment of the miR-204 seed sequence (arrow) and the wild-type (WT) rat Mafa 3′ UTR target sequence (bold) and the mutated (M) target sequence (gray). (e) miR-204–directed repression of the luciferase reporter gene bearing the WT or mutant Mafa 3′ UTR segments as assessed 24 h after cotransfecting INS-1 cells with the wild-type or mutant reporter plasmids. All data are shown as the mean ± s.e.m. of three independent experiments, and one representative immunoblot is shown. *P < 0.05, **P < 0.01 (Student’s t test). NS, not significant.

Journal: Nature medicine

Article Title: Thioredoxin-interacting protein regulates insulin transcription through microRNA-204.

doi: 10.1038/nm.3287

Figure Lengend Snippet: Figure 3 MAFA as a target of miR-204. (a,b) The effects of miR-204 on Mafa mRNA (a) and protein (b) expression as assessed by qRT-PCR and immunoblotting. (c) Changes in the Mafa occupancy of the insulin promoter in response to miR-204 as measured by ChIP. IgG control results are shown on the right. Gapdh, glyceraldehyde 3-phosphate dehydrogenase. (d) Alignment of the miR-204 seed sequence (arrow) and the wild-type (WT) rat Mafa 3′ UTR target sequence (bold) and the mutated (M) target sequence (gray). (e) miR-204–directed repression of the luciferase reporter gene bearing the WT or mutant Mafa 3′ UTR segments as assessed 24 h after cotransfecting INS-1 cells with the wild-type or mutant reporter plasmids. All data are shown as the mean ± s.e.m. of three independent experiments, and one representative immunoblot is shown. *P < 0.05, **P < 0.01 (Student’s t test). NS, not significant.

Article Snippet: Five micrograms of rabbit antibody to MAFA (A300611A, Bethyl Laboratories, Montgomery, TX) or normal rabbit IgG (sc2027, Santa Cruz) were used for immuno precipitation, and purified DNA fragments were quantified by qPCR with the primers listed in Supplementary Table 2.

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Control, Sequencing, Luciferase, Mutagenesis

Figure 4 The effects of TXNIP on MAFA and insulin production. (a–c) TXNIP-overexpressing INS-TXNIP cells analyzed for Mafa mRNA by qRT-PCR (a), Mafa protein by immunoblotting (b) and Mafa occupancy of the insulin promoter by ChIP (c) compared to INS-LacZ control cells. IgG control is shown on the right. (d) MAFA mRNA expression, assessed by qRT-PCR, in human islets that were transfected with a TXNIP expression plasmid or LacZ. (e,f) Insulin mRNA, as assessed by qRT-PCR, in INS-TXNIP cells (e) and human islets transfected with TXNIP (f). (g,h) Insulin content (g) and insulin secretion (h) assessed by ELISA in INS-TXNIP and INS-LacZ control cells. (i) Islet insulin content measured by ELISA in primary islets of TXNIP-deficient HcB-19 and control C3H mice. (j) Insulin content, measured by ELISA and normalized for DNA content, in control C3H and TXNIP-deficient HcB-19 mouse islets that were transfected with scrambled control microRNA or precursor miR-204 and analyzed 2 d later. All data are shown as the mean ± s.e.m. of three independent experiments. *P < 0.05, **P < 0.01 (Student’s t test (a–h) or analysis of variance (j)). NS, not significant.

Journal: Nature medicine

Article Title: Thioredoxin-interacting protein regulates insulin transcription through microRNA-204.

doi: 10.1038/nm.3287

Figure Lengend Snippet: Figure 4 The effects of TXNIP on MAFA and insulin production. (a–c) TXNIP-overexpressing INS-TXNIP cells analyzed for Mafa mRNA by qRT-PCR (a), Mafa protein by immunoblotting (b) and Mafa occupancy of the insulin promoter by ChIP (c) compared to INS-LacZ control cells. IgG control is shown on the right. (d) MAFA mRNA expression, assessed by qRT-PCR, in human islets that were transfected with a TXNIP expression plasmid or LacZ. (e,f) Insulin mRNA, as assessed by qRT-PCR, in INS-TXNIP cells (e) and human islets transfected with TXNIP (f). (g,h) Insulin content (g) and insulin secretion (h) assessed by ELISA in INS-TXNIP and INS-LacZ control cells. (i) Islet insulin content measured by ELISA in primary islets of TXNIP-deficient HcB-19 and control C3H mice. (j) Insulin content, measured by ELISA and normalized for DNA content, in control C3H and TXNIP-deficient HcB-19 mouse islets that were transfected with scrambled control microRNA or precursor miR-204 and analyzed 2 d later. All data are shown as the mean ± s.e.m. of three independent experiments. *P < 0.05, **P < 0.01 (Student’s t test (a–h) or analysis of variance (j)). NS, not significant.

Article Snippet: Five micrograms of rabbit antibody to MAFA (A300611A, Bethyl Laboratories, Montgomery, TX) or normal rabbit IgG (sc2027, Santa Cruz) were used for immuno precipitation, and purified DNA fragments were quantified by qPCR with the primers listed in Supplementary Table 2.

Techniques: Quantitative RT-PCR, Western Blot, Control, Expressing, Transfection, Plasmid Preparation, Enzyme-linked Immunosorbent Assay