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Image Search Results
Journal: Biotechniques
Article Title: Proximity ligation assay to study protein–protein interactions of proteins on two different cells
doi: 10.2144/btn-2018-0049
Figure Lengend Snippet: Expanded regions of (A) PPI without treatment showing red fluorescence dots. Nuclei are shown stained blue with DAPI. (B) Bright field image showing HFLS-RA cells and T cells with PLA red fluorescence dots. (C & D) PLA in the presence of compound SFTI-a at a concentration of 100 μM. Notice the decrease in the number of red dots, indicating PPI inhibition between CD2 and CD58. HFLS-RA: Human fibroblast-like synoviocyte-rheumatoid arthritis; PLA: Proximity ligation assay; PPI: Protein–protein interaction.
Article Snippet: Specific primary antibodies against CD58 (sc-6983, RRID: AB_2076111) c-terminus goat polyclonal and
Techniques: Fluorescence, Staining, Concentration Assay, Inhibition, Proximity Ligation Assay