mab6158 Search Results


anti ucp1  (R&D Systems)
95
R&D Systems anti ucp1
Anti Ucp1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mab6158/Human%2FMouse+UCP1+Antibody/bio_rxiv__2024__07__01__601572-55-35-37
Average 95 stars, based on 1 article reviews
anti ucp1 - by Bioz Stars, 2026-11
95/100 stars
  Buy from Supplier
ucp1  (Novus Biologicals)
95
Novus Biologicals ucp1
Effect of the differentiation protocols on white and brown adipocyte markers and lipid metabolism gene expression. Human adipose-derived stem cells were differentiated for 21 days with five adipogenic differentiation protocols. Relative mRNA expression of genes (a) CIDEA , (b) LEPTIN , (d) <t>UCP1</t> , and (e) UCP2 measured by RT-qPCR. (c) Secretion of leptin to the cell culture media. (f) Protein levels of UCP1 and vinculin were analyzed by western blotting and quantification of UCP1 level relative to vinculin. (g) Relative mRNA expression of UCP1 in in vitro -differentiated adipocytes, white adipose tissue (WAT), and mature adipocyte fraction (MAF) of the same subjects measured by RT-qPCR. Relative mRNA expression of genes (h) ACADM , (i) ACADS , and (j) LIPE measured by RT-qPCR. Gene expression results were normalized to the expression of reference genes IPO8 and GUSB using qBASE software. (k) Basal and isoproterenol-stimulated free glycerol release in media. Statistical analysis was performed using one-way ANOVA followed by Tukey's post hoc analysis ( n = 6): ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001. Error bars are shown as SEM. The statistical differences in (a), (e), and (f) indicate a comparison with all the other protocols.
Ucp1, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mab6158/Human%2FMouse+UCP1+Antibody/pmc09005291-109-28-32
Average 95 stars, based on 1 article reviews
ucp1 - by Bioz Stars, 2026-11
95/100 stars
  Buy from Supplier

Image Search Results


Effect of the differentiation protocols on white and brown adipocyte markers and lipid metabolism gene expression. Human adipose-derived stem cells were differentiated for 21 days with five adipogenic differentiation protocols. Relative mRNA expression of genes (a) CIDEA , (b) LEPTIN , (d) UCP1 , and (e) UCP2 measured by RT-qPCR. (c) Secretion of leptin to the cell culture media. (f) Protein levels of UCP1 and vinculin were analyzed by western blotting and quantification of UCP1 level relative to vinculin. (g) Relative mRNA expression of UCP1 in in vitro -differentiated adipocytes, white adipose tissue (WAT), and mature adipocyte fraction (MAF) of the same subjects measured by RT-qPCR. Relative mRNA expression of genes (h) ACADM , (i) ACADS , and (j) LIPE measured by RT-qPCR. Gene expression results were normalized to the expression of reference genes IPO8 and GUSB using qBASE software. (k) Basal and isoproterenol-stimulated free glycerol release in media. Statistical analysis was performed using one-way ANOVA followed by Tukey's post hoc analysis ( n = 6): ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001. Error bars are shown as SEM. The statistical differences in (a), (e), and (f) indicate a comparison with all the other protocols.

Journal: Stem Cells International

Article Title: Preventing White Adipocyte Browning during Differentiation In Vitro : The Effect of Differentiation Protocols on Metabolic and Mitochondrial Phenotypes

doi: 10.1155/2022/3308194

Figure Lengend Snippet: Effect of the differentiation protocols on white and brown adipocyte markers and lipid metabolism gene expression. Human adipose-derived stem cells were differentiated for 21 days with five adipogenic differentiation protocols. Relative mRNA expression of genes (a) CIDEA , (b) LEPTIN , (d) UCP1 , and (e) UCP2 measured by RT-qPCR. (c) Secretion of leptin to the cell culture media. (f) Protein levels of UCP1 and vinculin were analyzed by western blotting and quantification of UCP1 level relative to vinculin. (g) Relative mRNA expression of UCP1 in in vitro -differentiated adipocytes, white adipose tissue (WAT), and mature adipocyte fraction (MAF) of the same subjects measured by RT-qPCR. Relative mRNA expression of genes (h) ACADM , (i) ACADS , and (j) LIPE measured by RT-qPCR. Gene expression results were normalized to the expression of reference genes IPO8 and GUSB using qBASE software. (k) Basal and isoproterenol-stimulated free glycerol release in media. Statistical analysis was performed using one-way ANOVA followed by Tukey's post hoc analysis ( n = 6): ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001. Error bars are shown as SEM. The statistical differences in (a), (e), and (f) indicate a comparison with all the other protocols.

Article Snippet: The primary antibodies used were human OXPHOS cocktail (1 : 1000, OXPHOS cocktail Abcam, ab110411), vinculin (1 : 2500, Abcam ab129002), porin/VDAC1 (1 : 1000, Abcam ab15895), and UCP1 (1 : 1000, Novus Biologicals MAB6158).

Techniques: Gene Expression, Derivative Assay, Expressing, Quantitative RT-PCR, Cell Culture, Western Blot, In Vitro, Software, Comparison