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Image Search Results
Journal: Frontiers in Immunology
Article Title: Discovery of functionally distinct anti-C7 monoclonal antibodies and stratification of anti-nicotinic AChR positive Myasthenia Gravis patients
doi: 10.3389/fimmu.2022.968206
Figure Lengend Snippet: (A) Schematic representation of Myasthenia Gravis disease biology driven by anti-AChR autoantibodies. (B–F) AF647 α-Bungarotoxin (in red) and nuclear stain (in blue) binding to cells transfected with AChRα1β1δϵ+Rapsyn (B) , AChRα1β1δϵ (C) , AChRα1β1 (D) , AChRδϵ (E) , Untransfected control cells (F, G) IgG, IgA, and IgM quantification of immunoglobulin-depleted vs. non-depleted NHS. (H) Haemolytic activity of immunoglobulin-depleted vs. non-depleted NHS. (I) Binding profile (IgG+IgA+IgM binding) of non-depleted NHS to transfected cells (blue: nuclear stain; red: AF647 anti-human IgG+IgA+IgM). (J) Binding profile (IgG+IgA+IgM binding) of immunoglobulin (Ig)-depleted NHS to transfected cells (blue: nuclear stain; red: AF647 anti-human IgG+IgA+IgM). (K) AChR decrease in AChR+Rapsyn-transfected cells incubated with tool anti-AChR antibody mAb35 in the presence of Ig-depleted NHS (n = 30) (One-way ANOVA (GraphPad Prism), Tukey’s multiple comparisons test). (L) Increased MAC deposition in AChR positive cells, incubated with tool anti-AChR antibody mAb35 in the presence of Ig-depleted NHS (n = 30) (One-way ANOVA (GraphPad Prism), Tukey’s multiple comparisons test). All NHS used and referred to in the remainder of the paper is Ig-depleted NHS. ****p <0.0001.
Article Snippet: A 10μg/ml
Techniques: Staining, Binding Assay, Transfection, Control, Activity Assay, Incubation