mab247 Search Results


human il  (R&D Systems)
96
R&D Systems human il
Human Il, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human il/product/R&D Systems
Average 96 stars, based on 1 article reviews
human il - by Bioz Stars, 2026-06
96/100 stars
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mab247  (R&D Systems)
93
R&D Systems mab247
Fig. 2 Results of second-step screening process of IL-15-CAT fusions when expressed in E. coli DH5a. The vertical axis (solubility test) corresponds to the ELISA absorbance of cell extracts incubated in plates coated with <t>Mab247,</t> revealed with an anti-CAT polyclonal antibody. The horizontal axis (functionality test) corresponds to the ELISA absorbance of cell extracts incubated in plates coated with IL-15Ra and revealed with Mab247. Filled squares represent clones selected at cam ,400 mM; filled circle, clones selected at cam 800 mM; filled triangle, clones selected at cam 1200 mM; open square, clones selected at cam 1500 mM.
Mab247, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mab247/product/R&D Systems
Average 93 stars, based on 1 article reviews
mab247 - by Bioz Stars, 2026-06
93/100 stars
  Buy from Supplier

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Fig. 2 Results of second-step screening process of IL-15-CAT fusions when expressed in E. coli DH5a. The vertical axis (solubility test) corresponds to the ELISA absorbance of cell extracts incubated in plates coated with Mab247, revealed with an anti-CAT polyclonal antibody. The horizontal axis (functionality test) corresponds to the ELISA absorbance of cell extracts incubated in plates coated with IL-15Ra and revealed with Mab247. Filled squares represent clones selected at cam ,400 mM; filled circle, clones selected at cam 800 mM; filled triangle, clones selected at cam 1200 mM; open square, clones selected at cam 1500 mM.

Journal: Protein engineering, design & selection : PEDS

Article Title: Evolution of interleukin-15 for higher E. coli expression and solubility.

doi: 10.1093/protein/gzq107

Figure Lengend Snippet: Fig. 2 Results of second-step screening process of IL-15-CAT fusions when expressed in E. coli DH5a. The vertical axis (solubility test) corresponds to the ELISA absorbance of cell extracts incubated in plates coated with Mab247, revealed with an anti-CAT polyclonal antibody. The horizontal axis (functionality test) corresponds to the ELISA absorbance of cell extracts incubated in plates coated with IL-15Ra and revealed with Mab247. Filled squares represent clones selected at cam ,400 mM; filled circle, clones selected at cam 800 mM; filled triangle, clones selected at cam 1200 mM; open square, clones selected at cam 1500 mM.

Article Snippet: To screen mutants for their solubility, a sandwich ELISA test was used with Mab247 (anti-human IL-15 IgG, R&D system), dilution 1 mg/ml, as the capture antibody and a two-step detection system with a polyclonal rabbit anti-CAT IgG (Sigma) and a goat anti-rabbit IgG conjugated with peroxidase (Sigma).

Techniques: Solubility, Enzyme-linked Immunosorbent Assay, Incubation, Clone Assay