mab221 Search Results


mab221  (R&D Systems)
92
R&D Systems mab221
Mab221, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mab221/product/R&D Systems
Average 92 stars, based on 1 article reviews
mab221 - by Bioz Stars, 2026-04
92/100 stars
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pdgf aa  (R&D Systems)
90
R&D Systems pdgf aa
FIG. 1. Binding of bFGF and <t>PDGF-AA</t> to plastic-immobilized NG2 fragments. Increasing concentrations of bFGF (A) or PDGF-AA (B) were added to microtiter wells coated with NG2 frag- ments or BSA at a concentration of 3 mg/ ml. Bound bFGF and PDGF-AA were de- tected with growth factor-specific poly- <t>clonal</t> <t>antibodies.</t> The binding assay was performed as described under “Experi- mental Procedures.” Curves in NG2- coated wells represent the best fit deter- mined by nonlinear regression analysis.
Pdgf Aa, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pdgf aa/product/R&D Systems
Average 90 stars, based on 1 article reviews
pdgf aa - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier

Image Search Results


FIG. 1. Binding of bFGF and PDGF-AA to plastic-immobilized NG2 fragments. Increasing concentrations of bFGF (A) or PDGF-AA (B) were added to microtiter wells coated with NG2 frag- ments or BSA at a concentration of 3 mg/ ml. Bound bFGF and PDGF-AA were de- tected with growth factor-specific poly- clonal antibodies. The binding assay was performed as described under “Experi- mental Procedures.” Curves in NG2- coated wells represent the best fit deter- mined by nonlinear regression analysis.

Journal: The Journal of biological chemistry

Article Title: High-affinity binding of basic fibroblast growth factor and platelet-derived growth factor-AA to the core protein of the NG2 proteoglycan.

doi: 10.1074/jbc.274.24.16831

Figure Lengend Snippet: FIG. 1. Binding of bFGF and PDGF-AA to plastic-immobilized NG2 fragments. Increasing concentrations of bFGF (A) or PDGF-AA (B) were added to microtiter wells coated with NG2 frag- ments or BSA at a concentration of 3 mg/ ml. Bound bFGF and PDGF-AA were de- tected with growth factor-specific poly- clonal antibodies. The binding assay was performed as described under “Experi- mental Procedures.” Curves in NG2- coated wells represent the best fit deter- mined by nonlinear regression analysis.

Article Snippet: Goat polyclonal antibodies against human bFGF, EGF, PDGF-AA, PDGF-BB, TGF-b1, and VEGF165 were purchased from R&D Systems (Minneapolis, MN).

Techniques: Binding Assay, Concentration Assay

FIG. 2. Inhibition of binding of bFGF or PDGF-AA to immobilized NG2D2 or NG2D3 by soluble NG2 fragments. Microtiter wells coated with NG2/D2 (A) or NG2/D3 (B) were incu- bated with 15 nM FGF (f) or 15 nM PDGF-AA (G) in the presence of increas- ing concentrations of NG2/D2 (A) or NG2/D3 (B), respectively. Bound growth factor was detected with growth factor- specific polyclonal antibodies. The OD at 450 nm obtained with bound growth fac- tors in the absence of soluble NG2 frag- ments was set at 100%. Each point repre- sents the mean 6 S.D. of duplicate values from three separate experiments.

Journal: The Journal of biological chemistry

Article Title: High-affinity binding of basic fibroblast growth factor and platelet-derived growth factor-AA to the core protein of the NG2 proteoglycan.

doi: 10.1074/jbc.274.24.16831

Figure Lengend Snippet: FIG. 2. Inhibition of binding of bFGF or PDGF-AA to immobilized NG2D2 or NG2D3 by soluble NG2 fragments. Microtiter wells coated with NG2/D2 (A) or NG2/D3 (B) were incu- bated with 15 nM FGF (f) or 15 nM PDGF-AA (G) in the presence of increas- ing concentrations of NG2/D2 (A) or NG2/D3 (B), respectively. Bound growth factor was detected with growth factor- specific polyclonal antibodies. The OD at 450 nm obtained with bound growth fac- tors in the absence of soluble NG2 frag- ments was set at 100%. Each point repre- sents the mean 6 S.D. of duplicate values from three separate experiments.

Article Snippet: Goat polyclonal antibodies against human bFGF, EGF, PDGF-AA, PDGF-BB, TGF-b1, and VEGF165 were purchased from R&D Systems (Minneapolis, MN).

Techniques: Inhibition, Binding Assay

FIG. 3. Binding of NG2 fragments to plastic-immobilized growth factors. NG2/EC minus GAGs was diluted at 20 (black columns) and 100 (gray columns) ng/ml and added to microtiter wells coated with bFGF, PDGF-AA, PDGF-BB, TGF-b1, or BSA at a concentration of 2 mg/ml. The inset shows binding of D3 at a concentration of 40 (black columns) or 200 (gray columns) ng/ml to bFGF, PDGF-AA, and BSA-coated wells (2 mg/ml). Bound NG2 fragments were detected with NG2/ EC-specific or NG2/D3-specific polyclonal antibodies. The data represent the mean 6 S.D. of duplicate values from four separate experiments.

Journal: The Journal of biological chemistry

Article Title: High-affinity binding of basic fibroblast growth factor and platelet-derived growth factor-AA to the core protein of the NG2 proteoglycan.

doi: 10.1074/jbc.274.24.16831

Figure Lengend Snippet: FIG. 3. Binding of NG2 fragments to plastic-immobilized growth factors. NG2/EC minus GAGs was diluted at 20 (black columns) and 100 (gray columns) ng/ml and added to microtiter wells coated with bFGF, PDGF-AA, PDGF-BB, TGF-b1, or BSA at a concentration of 2 mg/ml. The inset shows binding of D3 at a concentration of 40 (black columns) or 200 (gray columns) ng/ml to bFGF, PDGF-AA, and BSA-coated wells (2 mg/ml). Bound NG2 fragments were detected with NG2/ EC-specific or NG2/D3-specific polyclonal antibodies. The data represent the mean 6 S.D. of duplicate values from four separate experiments.

Article Snippet: Goat polyclonal antibodies against human bFGF, EGF, PDGF-AA, PDGF-BB, TGF-b1, and VEGF165 were purchased from R&D Systems (Minneapolis, MN).

Techniques: Binding Assay, Concentration Assay