mab219 Search Results


mab219  (R&D Systems)
94
R&D Systems mab219
Mab219, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mab219/product/R&D Systems
Average 94 stars, based on 1 article reviews
mab219 - by Bioz Stars, 2026-03
94/100 stars
  Buy from Supplier
il 12p70  (R&D Systems)
93
R&D Systems il 12p70
( a – d , f , h ) Isotype control, <t>anti-MR1,</t> <t>anti-IL-12,</t> anti-IL-15 and/or anti-IL-18 antibodies were added to a co-culture exposed to DENV ( a , d , n =3–6), IAV ( b , f , n =5–29) or HCV ( c , h , n =7–16). IFN-γ expression by MAIT cells (gated on live CD3 + CD8 + CD161 ++ Vα7.2 + cells) was analysed by flow cytometry and is shown relative to the isotype control. ( e , g , i ) <t>IL-12p70,</t> IL-15 and IL-18 levels secreted by virus-exposed APC's. ( e ) DENV-exposed DC's ( n =4) at 42 h. ( g ) IAV-exposed macrophages ( n =7) at 48 h. ( i ) HCV-exposed macrophages ( n =7) at 48 h. Data are representative from at least two independent experiments. Bars represent means±s.e.m. Statistical significance was determined with the Mann–Whitney test ( a – c , e , g , i ) or the Kruskal–Wallis test followed by the Dunns' test or the Mann–Whitney test ( d , f , h ). ns>0.05, * P 0.05, *** P ≤0.001, **** P ≤0.0001. ND, not detected.
Il 12p70, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/il 12p70/product/R&D Systems
Average 93 stars, based on 1 article reviews
il 12p70 - by Bioz Stars, 2026-03
93/100 stars
  Buy from Supplier

Image Search Results


( a – d , f , h ) Isotype control, anti-MR1, anti-IL-12, anti-IL-15 and/or anti-IL-18 antibodies were added to a co-culture exposed to DENV ( a , d , n =3–6), IAV ( b , f , n =5–29) or HCV ( c , h , n =7–16). IFN-γ expression by MAIT cells (gated on live CD3 + CD8 + CD161 ++ Vα7.2 + cells) was analysed by flow cytometry and is shown relative to the isotype control. ( e , g , i ) IL-12p70, IL-15 and IL-18 levels secreted by virus-exposed APC's. ( e ) DENV-exposed DC's ( n =4) at 42 h. ( g ) IAV-exposed macrophages ( n =7) at 48 h. ( i ) HCV-exposed macrophages ( n =7) at 48 h. Data are representative from at least two independent experiments. Bars represent means±s.e.m. Statistical significance was determined with the Mann–Whitney test ( a – c , e , g , i ) or the Kruskal–Wallis test followed by the Dunns' test or the Mann–Whitney test ( d , f , h ). ns>0.05, * P 0.05, *** P ≤0.001, **** P ≤0.0001. ND, not detected.

Journal: Nature Communications

Article Title: MAIT cells are activated during human viral infections

doi: 10.1038/ncomms11653

Figure Lengend Snippet: ( a – d , f , h ) Isotype control, anti-MR1, anti-IL-12, anti-IL-15 and/or anti-IL-18 antibodies were added to a co-culture exposed to DENV ( a , d , n =3–6), IAV ( b , f , n =5–29) or HCV ( c , h , n =7–16). IFN-γ expression by MAIT cells (gated on live CD3 + CD8 + CD161 ++ Vα7.2 + cells) was analysed by flow cytometry and is shown relative to the isotype control. ( e , g , i ) IL-12p70, IL-15 and IL-18 levels secreted by virus-exposed APC's. ( e ) DENV-exposed DC's ( n =4) at 42 h. ( g ) IAV-exposed macrophages ( n =7) at 48 h. ( i ) HCV-exposed macrophages ( n =7) at 48 h. Data are representative from at least two independent experiments. Bars represent means±s.e.m. Statistical significance was determined with the Mann–Whitney test ( a – c , e , g , i ) or the Kruskal–Wallis test followed by the Dunns' test or the Mann–Whitney test ( d , f , h ). ns>0.05, * P 0.05, *** P ≤0.001, **** P ≤0.0001. ND, not detected.

Article Snippet: Blocking antibodies against IL-12p40/70 (508804, eBioscience), IL-12p70 (MAB219-100, R&D), IL-15 (MAB2471, R&D) or IL-18 (D044-3, MBL International, USA), IFN-γ (14-7317-85, eBioscience) or MR1 (361102, Biolegend) were used at 5–10 μg ml −1 .

Techniques: Co-Culture Assay, Expressing, Flow Cytometry, MANN-WHITNEY

( a ) PBMC's from healthy individuals were directly stimulated for 24 h with IFN-α, IFN-β, IL-12, IL-15, IL-18 or indicated combinations thereof. IFN-γ expression by MAIT cells (gated on live CD3 + CD8 + CD161 ++ Vα7.2 + cells) was analysed by flow cytometry. ( b – d ) B18R (IFN-α/β neutralizing protein) or PBS control were added to the co-culture ( n =6–11). IFN-γ ( b ), CD69 ( c ) and Granzyme B ( d ) expression is shown relative to the control. Data are representative from at least two independent experiments. ( e ) PBMC's from healthy controls or HCV patients at baseline, during or end of treatment with either SOF+RBV or SOF+RBV/PEG-IFN were analysed by flow cytometry. CD69 expression on MAIT cells (gated on live CD3 + CD8 + CD161 ++ Vα7.2 + cells) was measured. Bars represent means±s.e.m. Statistical significance was determined with the Kruskal–Wallis test followed by the Dunns' test ( a ) or the Mann–Whitney test ( b – e ). ns>0.05, * P 0.05, *** P ≤0.001, **** P ≤0.0001. HC, healthy controls.

Journal: Nature Communications

Article Title: MAIT cells are activated during human viral infections

doi: 10.1038/ncomms11653

Figure Lengend Snippet: ( a ) PBMC's from healthy individuals were directly stimulated for 24 h with IFN-α, IFN-β, IL-12, IL-15, IL-18 or indicated combinations thereof. IFN-γ expression by MAIT cells (gated on live CD3 + CD8 + CD161 ++ Vα7.2 + cells) was analysed by flow cytometry. ( b – d ) B18R (IFN-α/β neutralizing protein) or PBS control were added to the co-culture ( n =6–11). IFN-γ ( b ), CD69 ( c ) and Granzyme B ( d ) expression is shown relative to the control. Data are representative from at least two independent experiments. ( e ) PBMC's from healthy controls or HCV patients at baseline, during or end of treatment with either SOF+RBV or SOF+RBV/PEG-IFN were analysed by flow cytometry. CD69 expression on MAIT cells (gated on live CD3 + CD8 + CD161 ++ Vα7.2 + cells) was measured. Bars represent means±s.e.m. Statistical significance was determined with the Kruskal–Wallis test followed by the Dunns' test ( a ) or the Mann–Whitney test ( b – e ). ns>0.05, * P 0.05, *** P ≤0.001, **** P ≤0.0001. HC, healthy controls.

Article Snippet: Blocking antibodies against IL-12p40/70 (508804, eBioscience), IL-12p70 (MAB219-100, R&D), IL-15 (MAB2471, R&D) or IL-18 (D044-3, MBL International, USA), IFN-γ (14-7317-85, eBioscience) or MR1 (361102, Biolegend) were used at 5–10 μg ml −1 .

Techniques: Expressing, Flow Cytometry, Co-Culture Assay, MANN-WHITNEY

Sorted MAIT cells (CD8 + CD161 ++ Vα7.2 + ) were rested or activated in a TCR-independent manner (IL-12+IL-18 stimulation, MAIT + ) for 24 h. ( a ) IFN-γ expression by MAIT cells (gated on live CD3 + CD8 + CD161 ++ Vα7.2 + cells) was analysed by flow cytometry. ( b , c ) Neat or diluted supernatants were transferred to hepatocyte lines infected with HCV expressing luciferase and viral replication measured 4 days post infection. ( d ) Supernatants were transferred to hepatocyte lines infected with HCV expressing luciferase repeated in the presence of an isotype control or anti-IFN-γ antibody. Data are representative from at least two independent experiments. Bars represent means±s.e.m. Statistical significance was determined with a Wilcoxon matched-paired test ( c ) or the Mann–Whitney test ( d ). ** P ≤0.01. −, unstimulated; luc, luciferase; RLU, relative light units; sup, supernatant.

Journal: Nature Communications

Article Title: MAIT cells are activated during human viral infections

doi: 10.1038/ncomms11653

Figure Lengend Snippet: Sorted MAIT cells (CD8 + CD161 ++ Vα7.2 + ) were rested or activated in a TCR-independent manner (IL-12+IL-18 stimulation, MAIT + ) for 24 h. ( a ) IFN-γ expression by MAIT cells (gated on live CD3 + CD8 + CD161 ++ Vα7.2 + cells) was analysed by flow cytometry. ( b , c ) Neat or diluted supernatants were transferred to hepatocyte lines infected with HCV expressing luciferase and viral replication measured 4 days post infection. ( d ) Supernatants were transferred to hepatocyte lines infected with HCV expressing luciferase repeated in the presence of an isotype control or anti-IFN-γ antibody. Data are representative from at least two independent experiments. Bars represent means±s.e.m. Statistical significance was determined with a Wilcoxon matched-paired test ( c ) or the Mann–Whitney test ( d ). ** P ≤0.01. −, unstimulated; luc, luciferase; RLU, relative light units; sup, supernatant.

Article Snippet: Blocking antibodies against IL-12p40/70 (508804, eBioscience), IL-12p70 (MAB219-100, R&D), IL-15 (MAB2471, R&D) or IL-18 (D044-3, MBL International, USA), IFN-γ (14-7317-85, eBioscience) or MR1 (361102, Biolegend) were used at 5–10 μg ml −1 .

Techniques: Expressing, Flow Cytometry, Infection, Luciferase, MANN-WHITNEY