Journal: Molecular and Cellular Biochemistry

Article Title: Low expression of dendritic cell-specific intercellular adhesion molecule-grabbing nonintegrin-related protein in lung cancer and significant correlations with brain metastasis and natural killer cells

doi: 10.1007/s11010-015-2465-4

Figure Lengend Snippet: Clinical data of the lung cancer patients in ELISA study

Article Snippet: Each plate was incubated at 37 °C for 2 h. Subsequently, PBST was used to wash the plates 3 times, and 100 μl anti-DC-SIGNR mouse monoclonal antibody (mAb, R&D Systems, Inc., USA, Catalog Number: MAB16211) diluted to a concentration of 0.5 μg/ml was added to each wells.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Molecular and Cellular Biochemistry

Article Title: Low expression of dendritic cell-specific intercellular adhesion molecule-grabbing nonintegrin-related protein in lung cancer and significant correlations with brain metastasis and natural killer cells

doi: 10.1007/s11010-015-2465-4

Figure Lengend Snippet: Low serum levels of DC-SIGNR in lung cancer patients. The scatter plot displays DC-SIGNR levels in serum samples from 173 lung cancer patients and 134 healthy controls were tested by enzyme-linked immunosorbent assay (ELISA). Each point represents the serum DC-SIGNR level of one sample. The horizontal lines indicate the median levels. Serum DC-SIGNR was significantly lower in lung cancer patients compared with healthy controls ( P = 0.0003) ( a ). Receiver operating characteristic (ROC) curve was used for serum DC-SIGNR in lung cancer prediction. A cut-off value of 3.8998 ng/L for DC-SIGNR to predict the presence of lung cancer with 78.03 % sensitivity and 49.25 % specificity (Area under curve (AUC) = 0.6212, P = 0.0003) ( b )

Article Snippet: Each plate was incubated at 37 °C for 2 h. Subsequently, PBST was used to wash the plates 3 times, and 100 μl anti-DC-SIGNR mouse monoclonal antibody (mAb, R&D Systems, Inc., USA, Catalog Number: MAB16211) diluted to a concentration of 0.5 μg/ml was added to each wells.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Molecular and Cellular Biochemistry

Article Title: Low expression of dendritic cell-specific intercellular adhesion molecule-grabbing nonintegrin-related protein in lung cancer and significant correlations with brain metastasis and natural killer cells

doi: 10.1007/s11010-015-2465-4

Figure Lengend Snippet: Serum DC-SIGNR levels were significantly correlated with brain metastasis. The serum levels of DC-SIGNR were not significantly different between metastasis and non-metastasis ( P = 0.3887) ( a ). Serum concentrations of DC-SIGNR were higher in brain metastasis than in non-metastasis ( P = 0.0283) ( b ). However, the serum DC-SIGNR levels were not significantly different between bone metastasis and non-metastasis ( P = 0.3841), lung metastasis and non-metastasis ( P = 0.6744), lymph node metastasis and non-metastasis ( P = 0.9880) ( b )

Article Snippet: Each plate was incubated at 37 °C for 2 h. Subsequently, PBST was used to wash the plates 3 times, and 100 μl anti-DC-SIGNR mouse monoclonal antibody (mAb, R&D Systems, Inc., USA, Catalog Number: MAB16211) diluted to a concentration of 0.5 μg/ml was added to each wells.

Techniques:

Journal: Molecular and Cellular Biochemistry

Article Title: Low expression of dendritic cell-specific intercellular adhesion molecule-grabbing nonintegrin-related protein in lung cancer and significant correlations with brain metastasis and natural killer cells

doi: 10.1007/s11010-015-2465-4

Figure Lengend Snippet: Correlations of serum levels of DC-SIGNR with NK, CD3, CD4, and CD8 levels. Serum DC-SIGNR levels correlated significantly with percent of NK cells in lung cancer patients ( P = 0.0017) ( a ). Serum levels of DC-SIGNR showed no significant correlation with CD3 ( b ), CD4 ( c ), and CD8 ( d )

Article Snippet: Each plate was incubated at 37 °C for 2 h. Subsequently, PBST was used to wash the plates 3 times, and 100 μl anti-DC-SIGNR mouse monoclonal antibody (mAb, R&D Systems, Inc., USA, Catalog Number: MAB16211) diluted to a concentration of 0.5 μg/ml was added to each wells.

Techniques:

Journal: Molecular and Cellular Biochemistry

Article Title: Low expression of dendritic cell-specific intercellular adhesion molecule-grabbing nonintegrin-related protein in lung cancer and significant correlations with brain metastasis and natural killer cells

doi: 10.1007/s11010-015-2465-4

Figure Lengend Snippet: Correlations of serum levels of DC-SIGNR with clinical data. Serum levels of DC-SIGNR showed no significant correlation with gender ( a ), age ( b , c ), cancer stage ( d ), and histologic subtype ( e , f )

Article Snippet: Each plate was incubated at 37 °C for 2 h. Subsequently, PBST was used to wash the plates 3 times, and 100 μl anti-DC-SIGNR mouse monoclonal antibody (mAb, R&D Systems, Inc., USA, Catalog Number: MAB16211) diluted to a concentration of 0.5 μg/ml was added to each wells.

Techniques:

Journal: Molecular and Cellular Biochemistry

Article Title: Low expression of dendritic cell-specific intercellular adhesion molecule-grabbing nonintegrin-related protein in lung cancer and significant correlations with brain metastasis and natural killer cells

doi: 10.1007/s11010-015-2465-4

Figure Lengend Snippet: Correlations of serum levels of DC-SIGNR with tumor markers. Serum levels of DC-SIGNR displayed no significant correlation with S100β ( a ) CEA ( b ), NSE ( c ), and CYFRA21-1 ( d )

Article Snippet: Each plate was incubated at 37 °C for 2 h. Subsequently, PBST was used to wash the plates 3 times, and 100 μl anti-DC-SIGNR mouse monoclonal antibody (mAb, R&D Systems, Inc., USA, Catalog Number: MAB16211) diluted to a concentration of 0.5 μg/ml was added to each wells.

Techniques:

Journal: Molecular and Cellular Biochemistry

Article Title: Low expression of dendritic cell-specific intercellular adhesion molecule-grabbing nonintegrin-related protein in lung cancer and significant correlations with brain metastasis and natural killer cells

doi: 10.1007/s11010-015-2465-4

Figure Lengend Snippet: IHC for DC-SIGNR expression in tissues of lung cancer tissues, tuberculosis patients, and normal tissues. IHC analysis was used to determine DC-SIGNR expression and arrows indicate positive staining. Areas in the black boxes of A, C, E, G, and I were enlarged below. DC-SIGNR expression was detected in lung tissues from tuberculosis patients ( a – b ). DC-SIGNR expression was detected in the lung tissues from lung cancer patients ( c – d ). DC-SIGNR expression in alveolar epithelia cells of normal lung tissues ( e – f ). DC-SIGNR expression in lymphatic endothelial cells exhibited strong positive ( g – h ). Normal lymph node tissues were used as a negative control ( i–j )

Article Snippet: Each plate was incubated at 37 °C for 2 h. Subsequently, PBST was used to wash the plates 3 times, and 100 μl anti-DC-SIGNR mouse monoclonal antibody (mAb, R&D Systems, Inc., USA, Catalog Number: MAB16211) diluted to a concentration of 0.5 μg/ml was added to each wells.

Techniques: Expressing, Staining, Negative Control

Journal: Molecular and Cellular Biochemistry

Article Title: Low expression of dendritic cell-specific intercellular adhesion molecule-grabbing nonintegrin-related protein in lung cancer and significant correlations with brain metastasis and natural killer cells

doi: 10.1007/s11010-015-2465-4

Figure Lengend Snippet: Semi-quantitative image analysis of DC-SIGNR expression in tissues. The graph displays a scatter plot of the levels of DC-SIGNR expression in lung tissues from lung cancer patients, tuberculosis patients, and normal controls. There was a statistical significance in IHC for DC-SIGNR expression between lung cancer tissues and normal lung tissues ( P = 0.0418), tuberculosis tissues and normal controls ( P = 0.0289). There was no significantly difference between lung cancer patients and tuberculosis patients ( P = 0.2696). DC-SIGNR expression in lung cancer patients was lower than in normal lung tissues

Article Snippet: Each plate was incubated at 37 °C for 2 h. Subsequently, PBST was used to wash the plates 3 times, and 100 μl anti-DC-SIGNR mouse monoclonal antibody (mAb, R&D Systems, Inc., USA, Catalog Number: MAB16211) diluted to a concentration of 0.5 μg/ml was added to each wells.

Techniques: Expressing