mab1556 Search Results


rat monoclonal anti podocalyxin  (R&D Systems)
93
R&D Systems rat monoclonal anti podocalyxin
Rat Monoclonal Anti Podocalyxin, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mab1556/pm41046267-298-88-92?v=R%26D+Systems
Average 93 stars, based on 1 article reviews
rat monoclonal anti podocalyxin - by Bioz Stars, 2026-07
93/100 stars
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anti podxl antibody  (R&D Systems)
99
R&D Systems anti podxl antibody
Fig. 1 | Naive and primed markers in the embryonic rosette. a, Summary of the main findings indicating the logic of signalling and embryonic and in vitro relationships. Downregulation of WNT allows the induction of OTX2, the transition from naive to rosette pluripotency and the acquisition of MEK responsivity. MEK signals drive commitment, lumenogenesis and further progression to primed pluripotency. Combined WNT and MEK inhibition captures rosette-stage pluripotency in vitro. WNT reverts rosette cells to naive pluripotency. b–j, Embryos of the indicated stages (blastocysts (b,e,h), rosettes (c,f,i,j) and early lumen (d,g)) stained for the indicated markers. The epiblast is encircled by a dashed white line. Arrows indicate the rosette centre (c, f and j visualized by <t>phalloidin</t> <t>staining</t> of the apical actin accumulation (in c and f)) or the lumen surrounded by <t>PODXL</t> (d and g). KLF4, NANOG, ESRRB, OTX2 and OCT6 were detected in 17 out of 17, 12 out of 12, 5 out of 5, 3 out of 17 and 0 out of 6 blastocysts, respectively, and in 14 out of 14, 18 out of 20, 7 out of 8, 38 out of 38 and 6 out of 12 rosettes, respectively. KLF4, NANOG, OTX2 and OCT6 were detected in 0 out of 15, 0 out of 4, 9 out of 9 and 22 out of 22 lumen-stage embryos, respectively. OCT6 was also detected in extraembryonic ectoderm (ExE), ESRRB in primitive endoderm (PrE) and OTX2 in lumen-stage PrE. k, Expression of Klf4, Otx2 and Oct6 relative to Oct4 (as an epiblast-specific marker) in embryos of the indicated stages, determined by RT–PCR. Each colour-coded dot represents a group of four embryos. ND, not detected. Adjustments of individual colour channels were performed equally for all related images (b–j). Scale bars, 20 µm (b–j).
Anti Podxl Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mab1556/pm32367046-375-21-24?v=R%26D+Systems
Average 99 stars, based on 1 article reviews
anti podxl antibody - by Bioz Stars, 2026-07
99/100 stars
  Buy from Supplier

Image Search Results


Fig. 1 | Naive and primed markers in the embryonic rosette. a, Summary of the main findings indicating the logic of signalling and embryonic and in vitro relationships. Downregulation of WNT allows the induction of OTX2, the transition from naive to rosette pluripotency and the acquisition of MEK responsivity. MEK signals drive commitment, lumenogenesis and further progression to primed pluripotency. Combined WNT and MEK inhibition captures rosette-stage pluripotency in vitro. WNT reverts rosette cells to naive pluripotency. b–j, Embryos of the indicated stages (blastocysts (b,e,h), rosettes (c,f,i,j) and early lumen (d,g)) stained for the indicated markers. The epiblast is encircled by a dashed white line. Arrows indicate the rosette centre (c, f and j visualized by phalloidin staining of the apical actin accumulation (in c and f)) or the lumen surrounded by PODXL (d and g). KLF4, NANOG, ESRRB, OTX2 and OCT6 were detected in 17 out of 17, 12 out of 12, 5 out of 5, 3 out of 17 and 0 out of 6 blastocysts, respectively, and in 14 out of 14, 18 out of 20, 7 out of 8, 38 out of 38 and 6 out of 12 rosettes, respectively. KLF4, NANOG, OTX2 and OCT6 were detected in 0 out of 15, 0 out of 4, 9 out of 9 and 22 out of 22 lumen-stage embryos, respectively. OCT6 was also detected in extraembryonic ectoderm (ExE), ESRRB in primitive endoderm (PrE) and OTX2 in lumen-stage PrE. k, Expression of Klf4, Otx2 and Oct6 relative to Oct4 (as an epiblast-specific marker) in embryos of the indicated stages, determined by RT–PCR. Each colour-coded dot represents a group of four embryos. ND, not detected. Adjustments of individual colour channels were performed equally for all related images (b–j). Scale bars, 20 µm (b–j).

Journal: Nature cell biology

Article Title: In vitro capture and characterization of embryonic rosette-stage pluripotency between naive and primed states.

doi: 10.1038/s41556-020-0508-x

Figure Lengend Snippet: Fig. 1 | Naive and primed markers in the embryonic rosette. a, Summary of the main findings indicating the logic of signalling and embryonic and in vitro relationships. Downregulation of WNT allows the induction of OTX2, the transition from naive to rosette pluripotency and the acquisition of MEK responsivity. MEK signals drive commitment, lumenogenesis and further progression to primed pluripotency. Combined WNT and MEK inhibition captures rosette-stage pluripotency in vitro. WNT reverts rosette cells to naive pluripotency. b–j, Embryos of the indicated stages (blastocysts (b,e,h), rosettes (c,f,i,j) and early lumen (d,g)) stained for the indicated markers. The epiblast is encircled by a dashed white line. Arrows indicate the rosette centre (c, f and j visualized by phalloidin staining of the apical actin accumulation (in c and f)) or the lumen surrounded by PODXL (d and g). KLF4, NANOG, ESRRB, OTX2 and OCT6 were detected in 17 out of 17, 12 out of 12, 5 out of 5, 3 out of 17 and 0 out of 6 blastocysts, respectively, and in 14 out of 14, 18 out of 20, 7 out of 8, 38 out of 38 and 6 out of 12 rosettes, respectively. KLF4, NANOG, OTX2 and OCT6 were detected in 0 out of 15, 0 out of 4, 9 out of 9 and 22 out of 22 lumen-stage embryos, respectively. OCT6 was also detected in extraembryonic ectoderm (ExE), ESRRB in primitive endoderm (PrE) and OTX2 in lumen-stage PrE. k, Expression of Klf4, Otx2 and Oct6 relative to Oct4 (as an epiblast-specific marker) in embryos of the indicated stages, determined by RT–PCR. Each colour-coded dot represents a group of four embryos. ND, not detected. Adjustments of individual colour channels were performed equally for all related images (b–j). Scale bars, 20 µm (b–j).

Article Snippet: For lumen assays, the live cultures were incubated for 1 h in a cell culture incubator with culture medium containing 1:500 anti-PODXL antibody (MAB1556, R&D Systems), followed by fixation and staining with secondary antibody as described above.

Techniques: In Vitro, Inhibition, Staining, Expressing, Marker, Reverse Transcription Polymerase Chain Reaction