m-0603 Search Results


adenine methyltransferase ecogii  (New England Biolabs)
94
New England Biolabs adenine methyltransferase ecogii
(A) Schematic representation of the two-step ChromSMF protocol . Cells are chemically permeabilized and incubated with M.CviPI (cytosine-MTase), a <t>methyltransferase</t> that deposits cytosine methylation at accessible GpCs across the genome (black spheres). The obligatory cofactor SAM is washed out to stop M.CviPI activity. Cells are then sequentially incubated with an antibody (Ab) targeting a histone modification, and the proteinA-Hia5 (adenine-MTase) fusion protein that will methylate adenines in proximity of the histone modification of interest (green spheres). Sequencing of the resulting DNA using Oxford Nanopore Technologies (ONT) enables simultaneous detection of histone modifications (green signal; mA) and transcription factor binding events (black signal; mC) at bulk and single-molecule resolution. (B) Example locus illustrating the simultaneous detection of H3K4me3 and chromatin accessibility at active promoters . Top panel: genome browser tracks displaying ChIP-seq enrichment for H3K4me3 (green) and DNase-seq signal (black). Lower panel: ChromSMF sample for H3K4me3. Average SMF signal (1 – mC%) of individual cytosines (black) and average smoothed mA signal (mA%; green; smoothing across 4 adenines). (C) Simultaneous detection of chromatin accessibility and H3K4me3 on individual DNA molecules at a locus with low ChIP-seq enrichment for H3K4me3 . Single-molecule stacks display either mA-H3K4me3 (green, left) or mC-chromatin accessibility (black, right) signal. Molecules are displayed in identical order in both panels and originate from the same sample. Single-molecule classification of H3K4me3 (green) and chromatin accessibility (black) are shown as stacked bar plots between the single-molecule stacks. Single-molecule quantification of total H3K4me3 and chromatin accessibility at the locus are shown at the bottom. (D) Simultaneous detection of chromatin accessibility and H3K4me3 on individual DNA molecules at a locus with high ChIP-seq enrichment for H3K4me3 . Single-molecule stacks display either mA-H3K4me3 (green, left) or mC-chromatin accessibility (black, right) signal. Molecules are displayed in identical order in both panels and originate from the same sample. Single-molecule classification of H3K4me3 (green) and chromatin accessibility (black) are shown as stacked bar plots between the single-molecule stacks. Single-molecule quantification of total H3K4me3 and chromatin accessibility at the locus are shown at the bottom.
Adenine Methyltransferase Ecogii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/adenine methyltransferase ecogii/product/New England Biolabs
Average 94 stars, based on 1 article reviews
adenine methyltransferase ecogii - by Bioz Stars, 2026-04
94/100 stars
  Buy from Supplier
m-0603  (Procell Inc)
90
Procell Inc m-0603
(A) Schematic representation of the two-step ChromSMF protocol . Cells are chemically permeabilized and incubated with M.CviPI (cytosine-MTase), a <t>methyltransferase</t> that deposits cytosine methylation at accessible GpCs across the genome (black spheres). The obligatory cofactor SAM is washed out to stop M.CviPI activity. Cells are then sequentially incubated with an antibody (Ab) targeting a histone modification, and the proteinA-Hia5 (adenine-MTase) fusion protein that will methylate adenines in proximity of the histone modification of interest (green spheres). Sequencing of the resulting DNA using Oxford Nanopore Technologies (ONT) enables simultaneous detection of histone modifications (green signal; mA) and transcription factor binding events (black signal; mC) at bulk and single-molecule resolution. (B) Example locus illustrating the simultaneous detection of H3K4me3 and chromatin accessibility at active promoters . Top panel: genome browser tracks displaying ChIP-seq enrichment for H3K4me3 (green) and DNase-seq signal (black). Lower panel: ChromSMF sample for H3K4me3. Average SMF signal (1 – mC%) of individual cytosines (black) and average smoothed mA signal (mA%; green; smoothing across 4 adenines). (C) Simultaneous detection of chromatin accessibility and H3K4me3 on individual DNA molecules at a locus with low ChIP-seq enrichment for H3K4me3 . Single-molecule stacks display either mA-H3K4me3 (green, left) or mC-chromatin accessibility (black, right) signal. Molecules are displayed in identical order in both panels and originate from the same sample. Single-molecule classification of H3K4me3 (green) and chromatin accessibility (black) are shown as stacked bar plots between the single-molecule stacks. Single-molecule quantification of total H3K4me3 and chromatin accessibility at the locus are shown at the bottom. (D) Simultaneous detection of chromatin accessibility and H3K4me3 on individual DNA molecules at a locus with high ChIP-seq enrichment for H3K4me3 . Single-molecule stacks display either mA-H3K4me3 (green, left) or mC-chromatin accessibility (black, right) signal. Molecules are displayed in identical order in both panels and originate from the same sample. Single-molecule classification of H3K4me3 (green) and chromatin accessibility (black) are shown as stacked bar plots between the single-molecule stacks. Single-molecule quantification of total H3K4me3 and chromatin accessibility at the locus are shown at the bottom.
M 0603, supplied by Procell Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/m-0603/product/Procell Inc
Average 90 stars, based on 1 article reviews
m-0603 - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier

Image Search Results


(A) Schematic representation of the two-step ChromSMF protocol . Cells are chemically permeabilized and incubated with M.CviPI (cytosine-MTase), a methyltransferase that deposits cytosine methylation at accessible GpCs across the genome (black spheres). The obligatory cofactor SAM is washed out to stop M.CviPI activity. Cells are then sequentially incubated with an antibody (Ab) targeting a histone modification, and the proteinA-Hia5 (adenine-MTase) fusion protein that will methylate adenines in proximity of the histone modification of interest (green spheres). Sequencing of the resulting DNA using Oxford Nanopore Technologies (ONT) enables simultaneous detection of histone modifications (green signal; mA) and transcription factor binding events (black signal; mC) at bulk and single-molecule resolution. (B) Example locus illustrating the simultaneous detection of H3K4me3 and chromatin accessibility at active promoters . Top panel: genome browser tracks displaying ChIP-seq enrichment for H3K4me3 (green) and DNase-seq signal (black). Lower panel: ChromSMF sample for H3K4me3. Average SMF signal (1 – mC%) of individual cytosines (black) and average smoothed mA signal (mA%; green; smoothing across 4 adenines). (C) Simultaneous detection of chromatin accessibility and H3K4me3 on individual DNA molecules at a locus with low ChIP-seq enrichment for H3K4me3 . Single-molecule stacks display either mA-H3K4me3 (green, left) or mC-chromatin accessibility (black, right) signal. Molecules are displayed in identical order in both panels and originate from the same sample. Single-molecule classification of H3K4me3 (green) and chromatin accessibility (black) are shown as stacked bar plots between the single-molecule stacks. Single-molecule quantification of total H3K4me3 and chromatin accessibility at the locus are shown at the bottom. (D) Simultaneous detection of chromatin accessibility and H3K4me3 on individual DNA molecules at a locus with high ChIP-seq enrichment for H3K4me3 . Single-molecule stacks display either mA-H3K4me3 (green, left) or mC-chromatin accessibility (black, right) signal. Molecules are displayed in identical order in both panels and originate from the same sample. Single-molecule classification of H3K4me3 (green) and chromatin accessibility (black) are shown as stacked bar plots between the single-molecule stacks. Single-molecule quantification of total H3K4me3 and chromatin accessibility at the locus are shown at the bottom.

Journal: bioRxiv

Article Title: ChromSMF: integrated profiling of histone modifications, protein-DNA interactions and DNA methylation on multi-kilobase DNA molecules

doi: 10.64898/2026.03.11.710921

Figure Lengend Snippet: (A) Schematic representation of the two-step ChromSMF protocol . Cells are chemically permeabilized and incubated with M.CviPI (cytosine-MTase), a methyltransferase that deposits cytosine methylation at accessible GpCs across the genome (black spheres). The obligatory cofactor SAM is washed out to stop M.CviPI activity. Cells are then sequentially incubated with an antibody (Ab) targeting a histone modification, and the proteinA-Hia5 (adenine-MTase) fusion protein that will methylate adenines in proximity of the histone modification of interest (green spheres). Sequencing of the resulting DNA using Oxford Nanopore Technologies (ONT) enables simultaneous detection of histone modifications (green signal; mA) and transcription factor binding events (black signal; mC) at bulk and single-molecule resolution. (B) Example locus illustrating the simultaneous detection of H3K4me3 and chromatin accessibility at active promoters . Top panel: genome browser tracks displaying ChIP-seq enrichment for H3K4me3 (green) and DNase-seq signal (black). Lower panel: ChromSMF sample for H3K4me3. Average SMF signal (1 – mC%) of individual cytosines (black) and average smoothed mA signal (mA%; green; smoothing across 4 adenines). (C) Simultaneous detection of chromatin accessibility and H3K4me3 on individual DNA molecules at a locus with low ChIP-seq enrichment for H3K4me3 . Single-molecule stacks display either mA-H3K4me3 (green, left) or mC-chromatin accessibility (black, right) signal. Molecules are displayed in identical order in both panels and originate from the same sample. Single-molecule classification of H3K4me3 (green) and chromatin accessibility (black) are shown as stacked bar plots between the single-molecule stacks. Single-molecule quantification of total H3K4me3 and chromatin accessibility at the locus are shown at the bottom. (D) Simultaneous detection of chromatin accessibility and H3K4me3 on individual DNA molecules at a locus with high ChIP-seq enrichment for H3K4me3 . Single-molecule stacks display either mA-H3K4me3 (green, left) or mC-chromatin accessibility (black, right) signal. Molecules are displayed in identical order in both panels and originate from the same sample. Single-molecule classification of H3K4me3 (green) and chromatin accessibility (black) are shown as stacked bar plots between the single-molecule stacks. Single-molecule quantification of total H3K4me3 and chromatin accessibility at the locus are shown at the bottom.

Article Snippet: For sample 3 (mA-only), gDNA was treated exclusively with the adenine methyltransferase EcoGII (NEB, M0603S) in two consecutive 30 min incubations at 37°C with 25 U/μg DNA of EcoGII each, supplemented with 1.2 mM SAM, and omitting CpG and GpC methyltransferases.

Techniques: Incubation, Methylation, Activity Assay, Modification, Sequencing, Binding Assay, ChIP-sequencing