Journal: The Journal of Biological Chemistry

Article Title: Pore-forming moss protein bryoporin is structurally and mechanistically related to actinoporins from evolutionarily distant cnidarians

doi: 10.1016/j.jbc.2022.102455

Figure Lengend Snippet: Interaction of bryoporin with model lipid membranes. A , comparison of hemolytic activity of bryoporin, EqtII, and FraC at pH 7.4. The d ashed lines are fits of the logistic function and the midpoint was used to estimate EC 50 ( Table 1 ). B , hemolytic activity of bryoporin at different pH values. ( A and B ) show relative hemolysis ( i.e. , change in absorbance at 630 nm normalized to 1 after 20 min). Each measurement was repeated three times. Points represent mean value ± SD. C , SPR measurements of 500 nM bryoporin binding to lipid bilayers of various lipid compositions. The inset shows zoomed area, as marked. D , kinetics of calcein release from 20 μM LUVs of different lipid compositions induced by 100 nM bryoporin. The bold central line is an average of three measurements, shaded area shows SD. E , bryoporin binding to HeLa cells. 5 μg/ml of bryoporin was added to nontreated (control, left panel ) cells or cells pretreated with 20 mM MβCD ( central panel ) or 1.7 unit/ml SMase ( right panel ). Bryoporin was detected by fluorescently labeled antibody. Differential interference contrast images corresponding to each panel are shown below. The scale bar represents 20 μm. LUV, large unilamellar vesicle; SPR, surface plasmon resonance.

Article Snippet: Cells grown on glass coverslips were pretreated with 20 mM MβCD (Cyclolab), 1.7 unit/ml SMase from Staphylococcus aureus (Sigma), or DMEM/F12 medium without fetal calf serum as control at 37 °C for 30 min.

Techniques: Comparison, Activity Assay, Binding Assay, Control, Labeling, SPR Assay

Journal: Journal of Pharmaceutical Analysis

Article Title: Effects and translatomics characteristics of a small-molecule inhibitor of METTL3 against non-small cell lung cancer

doi: 10.1016/j.jpha.2023.04.009

Figure Lengend Snippet: In vivo effects and regulation of STM2457 on programmed death-ligand 1 (PD-L1) expression in non-small cell lung cancer (NSCLC). (A) The modeling and drug administration scheme of a pulmonary metastasis mouse model. (B) In the lung sections of a pulmonary metastasis mouse model, the effect of STM2457 on NSCLC progression and the regulation of STM2457 on the expression of both methyltransferase-like 3 (METTL3) and PD-L1 in vivo were evaluated using hematoxylin and eosin (H&E) staining and immunohistochemistry (IHC) staining, respectively. HP: hydroxypropyl.

Article Snippet: In in vivo experiments, STM2457 was dissolved in a 20% ( m / V ) 2-hydroxypropyl-beta-cyclodextrin (Glpbio, Montclair, CA, USA) vehicle as the working solution with a concentration of 5 mg/mL.

Techniques: In Vivo, Expressing, Staining, Immunohistochemistry