Journal: International Journal of Dentistry

Article Title: Acinar Cell Proliferation Promoted by BMP2 in Injured Mouse Parotid Gland: BMP2 Promotes Cell Proliferation in Parotid Gland

doi: 10.1155/2023/1765317

Figure Lengend Snippet: Mouse parotid acinar cells in primary culture. (a) Cell proliferative capacity of BMP2-added (100 ng/mL) and nonadded (control) groups after 48 hr of culture. The proliferative capacity of BMP2-added cultured cells was significantly increased compared to that in the control group. ∗∗ Indicates significance at P < 0.01, ∗ P < 0.05. Data were shown as mean ± SD. Three independent experiments were performed. (b) Protein expression of E-cadherin (an epithelial marker) and vimentin (a mesenchymal marker) in cultured cells of each group at 48 hr after the addition of BMP2. NIH3T3 (3T3) was used as a positive control for mesenchymal markers. RS: rat serum.

Article Snippet: NIH3T3 whole cell lysate (Novus Biologicals, Centennial, CO, USA) was used as a positive control for mesenchymal markers.

Techniques: Control, Cell Culture, Expressing, Marker, Positive Control

Journal: Lupus Science & Medicine

Article Title: Truncated isoforms of GPSM2 containing the GoLoco motif region promote CD4 + T-cell migration in SLE

doi: 10.1136/lupus-2022-000742

Figure Lengend Snippet: A truncated isoform of GPSM2 promotes CD4 + T-cell migration in SLE. (A) Representative example of western blot analysis of GPSM2 and the truncated 35 kDa GPSM2 isoform in CD4 + T cells from patients with SLE (SLE) and HCs. GPSM2 overexpressing lysate served as a positive ctrl (+); no protein served as a negative ctrl (−). (B) Comparison of GPSM2 expression in CD4 + cells from patients with SLE (n=10) and healthy individuals (n=9). (C) Expression of the truncated GPSM2 isoform in CD4 + cells from patients with SLE (n=10) and healthy individuals (n=9). (D) Schematic view of the experimental set-up of an in vitro T-cell migration assay. (E) Inhibition of the truncated GPSM2 isoform with blocking antibodies (block) or ctrl antibodies leads to decreased migration of CD4+ T cells from patients with SLE (n=9). Data are presented as mean±SEM. Significance was calculated using unpaired (B, C) or paired (E) Student’s t-test. ctrl, control; GPSM2, G-protein signalling modulator 2; HC, healthy control; n.s., not significant.

Article Snippet: GPSM2 overexpressing lysate served as a positive control (Novus Biologicals, Centennial, USA; cat. no. NBL1-11310).

Techniques: Migration, Western Blot, Comparison, Expressing, In Vitro, Cell Migration Assay, Inhibition, Blocking Assay, Control

Journal: The Journal of Physiology

Article Title: Bile acids induce necrosis in pancreatic stellate cells dependent on calcium entry and sodium‐driven bile uptake

doi: 10.1113/JP272774

Figure Lengend Snippet: A , mRNA expression of selected genes encoding bile acid transporters in human pancreatic stellate cells (hPSCs) and human hepatocytes. PCR products for the sodium–taurocholate cotransporting polypeptide (NTCP, slc10A1) and sodium independent transporters, OATP4A1 ( slco4A1 ) and OATP1B3 (slco1B3) , are present in human hepatocytes; hPSCs express slc10A1 and slco4A1 , but not slco1B3 . B , immunoblotting shows expression of NTCP in different tissues; lines from the left: mass marker, hPSC, four positive controls (human liver and mouse pancreas, liver and kidney), two negative controls (mouse spleen and lung). C , immunohistofluorescence (IHF) staining for NTCP in fixed mouse tissue sections: the pancreas (upper panel) and the liver (lower panel). From the left: DAPI (blue), NTCP (white), overlay images (white arrows indicate PSCs), and transmitted light images. Insets show corresponding staining in controls without primary antibody. D , double IHF staining for NTCP and the bradykinin receptor B2 (BDKRB2). From the upper left: DAPI (blue), NTCP (green), BDKRB2 (red), fluorescence overlay (PSCs are indicated by white arrows). E and F , the comparison of homological sequences of three Na + ‐dependent mouse transporters (NTCP, ASBT and SOAT) with immunogens used for generation of the antibodies for immunoblotting ( E ) and IHF ( F ). Red letters represent amino acids identical to those in the immunogen sequence; blue letters are similar amino acids, and black letters show amino acids that are different. Only mouse NTCP sequences share high sequence identity with the immunogens.

Article Snippet: Human liver tissue lysate was obtained from Novus Biologicals, Littleton, CO, USA.

Techniques: Expressing, Western Blot, Marker, Immunohistofluorescence, Staining, Fluorescence, Comparison, Sequencing