Journal: Frontiers in Cell and Developmental Biology

Article Title: A Blood Bank Standardized Production of Human Platelet Lysate for Mesenchymal Stromal Cell Expansion: Proteomic Characterization and Biological Effects

doi: 10.3389/fcell.2021.650490

Figure Lengend Snippet: hPL production scheme. The main production steps are highlighted: (A) starting from automatic separation of whole blood donation (WBD) in fresh frozen plasma (FFP), buffy coat (BC), and concentrated red cell (CRC) units. (B) Cryoprecipitate-poor plasma (CPP) is obtained by removing the cryoglobulin fraction of the plasma from FPP. (C) Platelet-rich plasma (PRP) is the product of the processing of six BC and one CPP. To obtain the platelet pool (PLT pool), platelet concentration was diluted to 1 × 10 6 PLT/μl with CPP. (D) Three batches (A–B–C18) of human platelet lysate (hPL) were produced. Each batch is the result of three PLT pools assembled and subjected to one or four freeze/thaw cycles. For each batch, both hPL with one freeze–thaw cycle (hPL1c) and hPL with four freeze–thaw cycles (hPL4c) were produced. After debris removal, hPL was sterile aliquoted and stored at –80°C until use. C, centrifugation; CR, cryoprecipitation; F, filtration; P, pooling; S, separation.

Article Snippet: Mononuclear cells are recovered by gradient separation (Lympholyte-H, Biosera) (400 g , 30 min, +22°C).

Techniques: Clinical Proteomics, Concentration Assay, Produced, Sterility, Centrifugation, Filtration