Journal: Acta biomaterialia

Article Title: Rapid endothelialization of small diameter vascular grafts by a bioactive integrin-binding ligand specifically targeting endothelial progenitor cells and endothelial cells

doi: 10.1016/j.actbio.2020.03.005

Figure Lengend Snippet: Binding specificity of LXW7 to ECFCs in blood circulation. (A) Beads displaying LXW7 were incubated with HECFCs, CD34− MNCs, HCASMCs and platelets for 2 h respectively. LXW7 efficiently supported binding of HECFCs (a) but did not support binding of CD34− MNCs (b), HCASMCs (c) and platelets (d). Scale bar =50 μm. (B) The numbers of different types of cells and platelets bound on each bead displaying LXW7 were quantified and statistical analyses were performed. Data were expressed as mean ± standard deviation: ***p < 0.001 (n = 5).

Article Snippet: For platelet-bead binding assay, 2 mL fresh PRP was added to an ultra-low attachment 35-mm Petri dish (Corning Incorporated) followed by LXW7-modified resin beads.

Techniques: Binding Assay, Incubation, Standard Deviation

Journal: Acta biomaterialia

Article Title: Rapid endothelialization of small diameter vascular grafts by a bioactive integrin-binding ligand specifically targeting endothelial progenitor cells and endothelial cells

doi: 10.1016/j.actbio.2020.03.005

Figure Lengend Snippet: ECFC attachment on LXW7-modified electrospun grafts. (A) Fluorescence images of remaining HECFC attachment on untreated grafts (a) and grafts modified with 10 nmol/cm2 LXW7 (b), 25 nmol/cm2 LXW7 (c), 50 nmol/cm2 LXW7 (d), 100 nmol/cm2 LXW7 (e), and 150 nmol/cm2 LXW7 (f) after 30 min incubation. (B) The numbers of HECFCs attached on grafts modified with different concentrations of LXW7 were quantified and statistical analyses were performed. Data were expressed as mean ± standard deviation: *p < 0.05, **p < 0.01, ***p < 0.001 (n = 5).

Article Snippet: For platelet-bead binding assay, 2 mL fresh PRP was added to an ultra-low attachment 35-mm Petri dish (Corning Incorporated) followed by LXW7-modified resin beads.

Techniques: Modification, Fluorescence, Incubation, Standard Deviation

Journal: Acta biomaterialia

Article Title: Rapid endothelialization of small diameter vascular grafts by a bioactive integrin-binding ligand specifically targeting endothelial progenitor cells and endothelial cells

doi: 10.1016/j.actbio.2020.03.005

Figure Lengend Snippet: X-ray photoelectron spectroscopy (XPS) wide spectra of electrospun grafts conjugated with LXW7.

Article Snippet: For platelet-bead binding assay, 2 mL fresh PRP was added to an ultra-low attachment 35-mm Petri dish (Corning Incorporated) followed by LXW7-modified resin beads.

Techniques: Spectroscopy

Journal: Acta biomaterialia

Article Title: Rapid endothelialization of small diameter vascular grafts by a bioactive integrin-binding ligand specifically targeting endothelial progenitor cells and endothelial cells

doi: 10.1016/j.actbio.2020.03.005

Figure Lengend Snippet: Amino acid analysis (AAA) of electrospun grafts conjugated with LXW7.

Article Snippet: For platelet-bead binding assay, 2 mL fresh PRP was added to an ultra-low attachment 35-mm Petri dish (Corning Incorporated) followed by LXW7-modified resin beads.

Techniques:

Journal: Acta biomaterialia

Article Title: Rapid endothelialization of small diameter vascular grafts by a bioactive integrin-binding ligand specifically targeting endothelial progenitor cells and endothelial cells

doi: 10.1016/j.actbio.2020.03.005

Figure Lengend Snippet: ECFC adhesion, proliferation, and differentiation on electrospun grafts conjugated with or without LXW7 treated. (A) Immunofluorescence staining results of remaining HECFCs attached on untreated (a) and LXW7-modified grafts (b) after 2 h incubation at day 1. The proliferation and differentiation of the attached HECFCs on untreated (c) and LXW7-modified grafts (d) at day 7. The numbers of cells attached on grafts at day 1 and day 7 (B), cell proliferation rates (C), and the number of CD31+ cells on grafts at day 1 and day 7 (D) were quantified and statistical analyses were performed. Data were expressed as mean ± standard deviation: **p < 0.01, ***p < 0.001 (n = 5).

Article Snippet: For platelet-bead binding assay, 2 mL fresh PRP was added to an ultra-low attachment 35-mm Petri dish (Corning Incorporated) followed by LXW7-modified resin beads.

Techniques: Immunofluorescence, Staining, Modification, Incubation, Standard Deviation

Journal: Acta biomaterialia

Article Title: Rapid endothelialization of small diameter vascular grafts by a bioactive integrin-binding ligand specifically targeting endothelial progenitor cells and endothelial cells

doi: 10.1016/j.actbio.2020.03.005

Figure Lengend Snippet: ECFC penetration on electrospun grafts conjugated with or without LXW7 treated. (A) HECFCs (green) penetrated into (a, c) untreated grafts and (b, d) LXW7 treated grafts. (c) and (d) are the magnifying images of part of (a) and (b). The area between the white lines were the sections of the grafts. (B) The numbers and (C) distribuation areas of HECFCs penetrated into grafts were quantified and statistical analyses were performed. Data were expressed as mean ± standard deviation: **p < 0.01 (n = 5).

Article Snippet: For platelet-bead binding assay, 2 mL fresh PRP was added to an ultra-low attachment 35-mm Petri dish (Corning Incorporated) followed by LXW7-modified resin beads.

Techniques: Standard Deviation

Journal: Acta biomaterialia

Article Title: Rapid endothelialization of small diameter vascular grafts by a bioactive integrin-binding ligand specifically targeting endothelial progenitor cells and endothelial cells

doi: 10.1016/j.actbio.2020.03.005

Figure Lengend Snippet: Platelet adhesion on electrospun grafts. (A) SEM revealed the morphology and adhesion of platelet on untreated electrospun grafts (a), PEG treated electrospun grafts (b) and LXW7-PEG treated electrospun grafts (c). White arrows showed the platelet aggregation.. (B) The numbers of platelets attached on grafts with different treatments were quantified and statistical analyses were performed. Data were expressed as mean ± standard deviation: **p < 0.01, (n = 5).

Article Snippet: For platelet-bead binding assay, 2 mL fresh PRP was added to an ultra-low attachment 35-mm Petri dish (Corning Incorporated) followed by LXW7-modified resin beads.

Techniques: Standard Deviation

Journal: Acta biomaterialia

Article Title: Rapid endothelialization of small diameter vascular grafts by a bioactive integrin-binding ligand specifically targeting endothelial progenitor cells and endothelial cells

doi: 10.1016/j.actbio.2020.03.005

Figure Lengend Snippet: Examination of explanted grafts. New capillary formation was exhibited on untreated grafts (A) and LXW7-modified grafts (B) at week 6 after implantation. Arrows in (B) indicate new capillary formation was present on the LXW7-modified grafts. Representative H&E staining of the cross sections of the vascular grafts at week 6 after implantation showed that the untreated graft had a significant amount of thrombus formation (C) and LXW7-modified graft exhibited a widely open lumen and no thrombus formation (D). (E) Immunostaining of cross sections of LXW7-modified grafts with mature EC marker CD31. (F) The patency of the grafts was examined at various time points. Each group at each time point included 6 animals.

Article Snippet: For platelet-bead binding assay, 2 mL fresh PRP was added to an ultra-low attachment 35-mm Petri dish (Corning Incorporated) followed by LXW7-modified resin beads.

Techniques: Modification, Staining, Immunostaining, Marker

Journal: Acta biomaterialia

Article Title: Rapid endothelialization of small diameter vascular grafts by a bioactive integrin-binding ligand specifically targeting endothelial progenitor cells and endothelial cells

doi: 10.1016/j.actbio.2020.03.005

Figure Lengend Snippet: Luminal cellularization of vascular grafts. En face DAPI staining was performed for longitudinal sections of the explanted grafts. Untreated grafts (A) and LXW7-modified grafts (B) at week 1 after implantation. Untreated grafts (C) and LXW7-modified grafts (D) at week 2 after implantation. Untreated grafts (E) and LXW7-modified grafts (F) at week 6 after implantation. Higher magnification images of representative areas on the luminal surfaces of the grafts in each group were shown in panels G-L correspondingly. (M) The nucleus coverage on grafts with different treatments and native rat carotid artery were quantified and statistical analyses were performed. Dashed lines indicate the anastomotic sites. Data were expressed as mean ± standard deviation: *p < 0.05, **p < 0.01 (n = 5).

Article Snippet: For platelet-bead binding assay, 2 mL fresh PRP was added to an ultra-low attachment 35-mm Petri dish (Corning Incorporated) followed by LXW7-modified resin beads.

Techniques: Staining, Modification, Standard Deviation

Journal: Acta biomaterialia

Article Title: Rapid endothelialization of small diameter vascular grafts by a bioactive integrin-binding ligand specifically targeting endothelial progenitor cells and endothelial cells

doi: 10.1016/j.actbio.2020.03.005

Figure Lengend Snippet: LXW7 enhanced EPC recruitment and migration and endothelialization on vascular grafts. En face immunostaining for CD31 (green) and CD34 (red) of proximal, middle, and distal portions of grafts. Untreated grafts (A-C) and LXW7-modified grafts (D-F) at week 1 after implantation. Untreated grafts (G-I) and LXW7-modified grafts (J-L) at week 2 after implantation. Untreated grafts (M-O) and LXW7-modified grafts (P-R) at week 6 after implantation. The ration of cells of different phenotypes at week 1 (S), week 2 (T) and week 6 (U) were quantified and statistical analyses were performed. Data were expressed as mean ± standard deviation: *p < 0.05 (n = 5). (V) Shape index of the cell elongation was quantified and statistical analyses were performed. Data were expressed as mean ± standard deviation: *p < 0.05 (n = 10).

Article Snippet: For platelet-bead binding assay, 2 mL fresh PRP was added to an ultra-low attachment 35-mm Petri dish (Corning Incorporated) followed by LXW7-modified resin beads.

Techniques: Migration, Immunostaining, Modification, Standard Deviation