Journal: mAbs

Article Title: Generation and in vivo characterization of a novel high-affinity human antibody targeting carcinoembryonic antigen

doi: 10.1080/19420862.2023.2217964

Figure Lengend Snippet: In vitro characterization of new anti-CEA antibodies. (a) SPR sensorgram of G9, F7, and F4 in a scFv format. K D values were calculated to be 640 nM, 50 nM, and 7.7 nM, respectively. (b) Flow cytometry analysis with the new antibodies in IgG format on CEA-expressing CT26 cells and on CEA-negative CT26 wild-type cells. (c) Immunofluorescence staining with IgG formats on the human colon adenocarcinoma xenograft LS174T. Anti-CEA antibodies were detected in green. Blood vessels were detected by CD31 staining (red). 20× magnification, scale bars = 100 μm.

Article Snippet: Immunofluorescence analysis was performed on LS174T and HT-29 xenografts harvested from mice, on patient-derived colon cancer samples, and on a human tissue microarray (Amsbio, T6235700–5).

Techniques: In Vitro, Flow Cytometry, Expressing, Immunofluorescence, Staining

Journal: mAbs

Article Title: Generation and in vivo characterization of a novel high-affinity human antibody targeting carcinoembryonic antigen

doi: 10.1080/19420862.2023.2217964

Figure Lengend Snippet: Ex vivo immunofluorescence-based biodistribution analysis. Immunofluorescence analysis assessed tumor targeting of new anti-CEA antibodies in IgG format. Two hundred micrograms of IgG-FITC were injected intravenously into LS174T-bearing mice. Tumors were excised 24 hours after injection. IgG-FITC was detected in green; blood vessels were detected through CD31 staining (red). 20× magnification, scale bars = 100 μm.

Article Snippet: Immunofluorescence analysis was performed on LS174T and HT-29 xenografts harvested from mice, on patient-derived colon cancer samples, and on a human tissue microarray (Amsbio, T6235700–5).

Techniques: Ex Vivo, Immunofluorescence, Injection, Staining

Journal: mAbs

Article Title: Generation and in vivo characterization of a novel high-affinity human antibody targeting carcinoembryonic antigen

doi: 10.1080/19420862.2023.2217964

Figure Lengend Snippet: Quantitative biodistribution with radiolabeled anti-CEA antibodies in diabody format. Quantitative biodistribution analysis of radio iodinated anti-CEA diabodies in BALB/c nude mice bearing subcutaneous LS174T colon adenocarcinomas. Organs were harvested 24 hours after intravenous injection, and radioactivity was quantified. Results are shown as the percentage of injected dose per gram (ID/g (%)). Error bars = SEM; n = 4.

Article Snippet: Immunofluorescence analysis was performed on LS174T and HT-29 xenografts harvested from mice, on patient-derived colon cancer samples, and on a human tissue microarray (Amsbio, T6235700–5).

Techniques: Injection, Radioactivity

Journal: British Journal of Cancer

Article Title: Identification of serum angiopoietin-2 as a biomarker for clinical outcome of colorectal cancer patients treated with bevacizumab-containing therapy

doi: 10.1038/sj.bjc.6605925

Figure Lengend Snippet: Expression of Ang-2 in CRC. ( A ) Illustration of the steps involved in laser microdissection of cryosections of CRC patients to obtain stromal (S) and tumour (T) material for RT–PCR amplification. The top row shows capture of tumour and the bottom row capture of stroma from the same section. ( B ) Gel electrophoresis of end point RT–PCR amplification products of Ang-2 and β -actin on material of a whole tumour section (W) or microdissected stromal or tumour areas of sections of five different CRC patients showing Ang-2 expression in W and S, but not in T. β -Actin served as a control. The bar chart shows the relative quantitative real-time PCR of Ang-2 levels normalised to β -actin expression using the Δ C t method. Bars represent the means of five patients with standard errors showing significant Ang-2 expression (100%) in the tumour stroma, but undetectable in the tumour cells themselves ( * ). Some expression (<10%) is also seen when whole sections were amplified, owing to the Ang-2 expressing stromal compartment. ( C ) End point RT–PCR analysis of xenograft tumours of human LS174T cells in nude mice analysed by species-specific amplification for human (tumour) and murine (stromal) Ang-2. Xenograft tumours show stromal-derived murine Ang-2, but not tumour-derived human Ang-2. The GAPDH was used as a species independent control. Human umbilical vein endothelial cells (HUVEC) served as a positive control for human Ang-2.

Article Snippet: The colon carcinoma cell lines LS174T, HT29, DLD-1 and SW948 were cultured in VLE RPMI 1640 (Biochrom, Berlin, Germany) supplemented with 10% FCS.

Techniques: Expressing, Laser Capture Microdissection, Reverse Transcription Polymerase Chain Reaction, Amplification, Nucleic Acid Electrophoresis, Control, Real-time Polymerase Chain Reaction, Derivative Assay, Positive Control