Journal: Frontiers in Immunology

Article Title: Tumor Apolipoprotein E is a key checkpoint blocking anti-tumor immunity in mouse melanoma

doi: 10.3389/fimmu.2022.991790

Figure Lengend Snippet: lrp8 is the most dominant receptor expressed on activated T cells and blocking lrp8 enhanced T-cell activation. (A) Expression of apoE receptors shows predominance of ldlr and lrp8 receptors on T cells, (B) lrp8, ldl and lrp1 receptors on dendritic cells, and (C) lrp1 receptors on macrophages as shown by quantitative real-time PCR (qPCR). Expression of ldlr and lrp8 was upregulated following T cell stimulation with CD3/CD28 beads. Expression of lrp8 was also upregulated following dendritic cell stimulation with TLR7/8. (D) The amount of IFNγ production from vaccinated splenocytes co-cultured with immunogenic WT B16 cells was quantified by ELISA assay. Results show that IFNγ production was significantly suppressed in the presence of the apoE agonist COG133, but suppression did not occur when splenocytes were harvested from lrp8 -/- mice. These findings suggest that T-cell function is at least partially inhibited by apoE through the lrp8 receptor pathway. Results are expressed as mean score ± SD. *p<0.05; **p<0.005; ***p<0.001, determined by unpaired two-tailed Student’s t-test.

Article Snippet: Gene-specific assays were Mm01307192_m1 for apoE, Mm00464608_m1 for Lrp1, Mm01328171_m1 for Lrp2, Mm00474030_m1 for Lrp8, Mm01177349_m1 for Ldlr, Mm00443298_m1 for Vldlr, Mm99999915_g1 for Gapdh (Life Technologies, Thermo Fisher).

Techniques: Blocking Assay, Activation Assay, Expressing, Real-time Polymerase Chain Reaction, Cell Stimulation, Cell Culture, Enzyme-linked Immunosorbent Assay, Cell Function Assay, Two Tailed Test

Journal: Thyroid

Article Title: Decreased Expression of Hepatic Low-Density Lipoprotein Receptor–Related Protein 1 in Hypothyroidism: A Novel Mechanism of Atherogenic Dyslipidemia in Hypothyroidism

doi: 10.1089/thy.2012.0457

Figure Lengend Snippet: Change in hepatic LRP1 expression in a hypothyroidism animal model. C57BL/6 mice were fed a normal diet (control group, n=6) or a low-iodine diet supplemented with 0.15% propylthiouracil (PTU/LI group, n=4) for 4 weeks. (A, B) Western blot analysis of LRP1 (β-chain) in liver samples. (C) RT-PCR quantification of LRP1 mRNA in liver samples. Results are normalized with β-actin protein or mRNA levels and are expressed as ratios relative to the control group. Data are mean±SE. *p<0.05 vs. control group. RT-PCR, real-time polymerase chain reaction; LRP1, low-density lipoprotein receptor–related protein 1; SE, standard error.

Article Snippet: Quantitative real-time polymerase chain reaction Quantitative real-time polymerase chain reaction analysis was performed using a TaqMan assay kit for LRP1 (Hs00233856_m1, Mm00464608_m1) and an ABI 7500 instrument (Applied Biosystems, Foster City, CA). β-Actin (Hs99999903_m1, Mm00607939_s1) was used as an invariant control.

Techniques: Expressing, Animal Model, Control, Western Blot, Reverse Transcription Polymerase Chain Reaction, Real-time Polymerase Chain Reaction

Journal: Thyroid

Article Title: Decreased Expression of Hepatic Low-Density Lipoprotein Receptor–Related Protein 1 in Hypothyroidism: A Novel Mechanism of Atherogenic Dyslipidemia in Hypothyroidism

doi: 10.1089/thy.2012.0457

Figure Lengend Snippet: Immunohistochemistry of LRP1 in the liver samples. L C57BL/6 mice were fed a normal diet (control group, n=6) or a low-iodine diet supplemented with 0.15% propylthiouracil (PTU/LI group, n=4) for 4 weeks. Three samples of each group are presented and LRP1 expression is shown by brown color. Bars indicate 100 μm. Color images are available online at www.liebertpub.com/thy

Article Snippet: Quantitative real-time polymerase chain reaction Quantitative real-time polymerase chain reaction analysis was performed using a TaqMan assay kit for LRP1 (Hs00233856_m1, Mm00464608_m1) and an ABI 7500 instrument (Applied Biosystems, Foster City, CA). β-Actin (Hs99999903_m1, Mm00607939_s1) was used as an invariant control.

Techniques: Immunohistochemistry, Control, Expressing

Journal: Thyroid

Article Title: Decreased Expression of Hepatic Low-Density Lipoprotein Receptor–Related Protein 1 in Hypothyroidism: A Novel Mechanism of Atherogenic Dyslipidemia in Hypothyroidism

doi: 10.1089/thy.2012.0457

Figure Lengend Snippet: Change in hepatic LRP1 expression after T3 treatment in animals. C57BL/6 mice were fed a low-iodine diet supplemented with 0.15% propylthiouracil (n=11). Various doses of T3 (0, 30, and 150 μg/kg of body weight) were administered daily to the mice through intraperitoneal injection for 7 days. (A, B) Western blot analysis of LRP1 (β-chain) in liver samples. (C) RT-PCR quantification of LRP1 mRNA in liver samples. Results are normalized with β-actin protein or mRNA levels and are expressed as ratios relative to 0 μg/kg. Data are mean±SE. *p<0.05 vs. 0 hour. T3, triiodothyronine.

Article Snippet: Quantitative real-time polymerase chain reaction Quantitative real-time polymerase chain reaction analysis was performed using a TaqMan assay kit for LRP1 (Hs00233856_m1, Mm00464608_m1) and an ABI 7500 instrument (Applied Biosystems, Foster City, CA). β-Actin (Hs99999903_m1, Mm00607939_s1) was used as an invariant control.

Techniques: Expressing, Injection, Western Blot, Reverse Transcription Polymerase Chain Reaction

Journal: Thyroid

Article Title: Decreased Expression of Hepatic Low-Density Lipoprotein Receptor–Related Protein 1 in Hypothyroidism: A Novel Mechanism of Atherogenic Dyslipidemia in Hypothyroidism

doi: 10.1089/thy.2012.0457

Figure Lengend Snippet: The effect of T3 on LRP1 expression in HepG2 cells. HepG2 cells incubated in CSS or unstripped FBS for 24 hours were treated with the indicated concentrations of T3 for 48 hours. (A) Western blot analysis of LRP1 (β-chain) in HepG2 cells (n=5 in each group). (B) Western blot analysis of LRP1 (β-chain) in HepG2 cells incubated in CSS (n=5 in each group). (C) RT-PCR quantification of LRP1 mRNA in HepG2 cells incubated in CSS (n=5 in each group). Results are normalized with β-actin protein or mRNA levels and are expressed as ratios relative to T3 0 nM in CSS. Data are mean±SE. *p<0.05 vs. T3 0 nM in CSS; †p<0.05 vs. T3 0.5 nM in CSS. CSS, charcoal-stripped fetal bovine serum; FBS, fetal bovine serum.

Article Snippet: Quantitative real-time polymerase chain reaction Quantitative real-time polymerase chain reaction analysis was performed using a TaqMan assay kit for LRP1 (Hs00233856_m1, Mm00464608_m1) and an ABI 7500 instrument (Applied Biosystems, Foster City, CA). β-Actin (Hs99999903_m1, Mm00607939_s1) was used as an invariant control.

Techniques: Expressing, Incubation, Western Blot, Reverse Transcription Polymerase Chain Reaction

Journal: Thyroid

Article Title: Decreased Expression of Hepatic Low-Density Lipoprotein Receptor–Related Protein 1 in Hypothyroidism: A Novel Mechanism of Atherogenic Dyslipidemia in Hypothyroidism

doi: 10.1089/thy.2012.0457

Figure Lengend Snippet: Uptake of lipid-conjugated ApoE3 by T3 in HepG2 cells. HepG2 cells incubated in CSS for 24 hours were treated with the indicated concentrations of T3 for 48 hours. Human recombinant ApoE3 conjugated with lipid was added to the culture medium and cells were incubated for 1 hour. (A) Western blot analysis of ApoE3 in HepG2 cells incubated with or without added ApoE3. (B) Western blot analysis of ApoE3 in HepG2 cells transfected with nontargeting negative siRNA (siCTRL) and siRNA targeting LRP1 (siLRP1). (C) Western blot analysis of ApoE3 in HepG2 cells transfected with siCTRL and siRNA targeting the LDL receptor (siLDLR). The human recombinant receptor–associated protein was used as a functional blocker of LRP1 and LDLR. The human recombinant receptor–associated protein was added to the culture medium before adding ApoE3. Three independent experiments were performed for the representative figures. siRNA, small interfering RNA; LDL, low-density lipoprotein; LDLR, LDL receptor.

Article Snippet: Quantitative real-time polymerase chain reaction Quantitative real-time polymerase chain reaction analysis was performed using a TaqMan assay kit for LRP1 (Hs00233856_m1, Mm00464608_m1) and an ABI 7500 instrument (Applied Biosystems, Foster City, CA). β-Actin (Hs99999903_m1, Mm00607939_s1) was used as an invariant control.

Techniques: Incubation, Recombinant, Western Blot, Transfection, Functional Assay, Small Interfering RNA

Journal: Alzheimer's Research & Therapy

Article Title: Photobiomodulation mitigates blood–brain barrier disruption in APP/PS1 mouse model of Alzheimer’s disease by activating the AMPK pathway

doi: 10.1186/s13195-025-01787-7

Figure Lengend Snippet: PBM Promotes the Transport Clearance of Aβ Deposition by the BBB in APP/PS1 Mice. A Western blot analysis of Aβ and LRP1 expression in mouse hippocampa tissue along with quantitative assessment. B Immunofluorescence staining in mouse cortex using anti-CD31 (green) and anti-LRP1 (red) antibodies (Scale bar: 50 μm). C Immunohistochemical staining for Aβ in the hippocampal region of mice (Scale bar: 100 μm). Arrows indicate Aβ deposition. D Immunofluorescence staining in the hippocampal region of mice using anti-Aβ (red) and anti-IBA1 (green) antibodies (scale bars: 200 μm and 20 μm, respectively). E Immunofluorescence staining in the hippocampal region of mice using anti-Aβ (red) and anti-GFAP (green) antibodies (scale bars: 200 μm and 50 μm, respectively). Data are presented as Mean ± SEM ( n = 3 per group). Comparisons indicate statistical significance relative to the Control group: ** p < 0.01, *** p < 0.001 and relative to the AD group: # p < 0.05

Article Snippet: LRP1 Antibody , Wuhan Boster (BM4098) , 1:200.

Techniques: Western Blot, Expressing, Immunofluorescence, Staining, Immunohistochemical staining, Control

Journal: Alzheimer's Research & Therapy

Article Title: Photobiomodulation mitigates blood–brain barrier disruption in APP/PS1 mouse model of Alzheimer’s disease by activating the AMPK pathway

doi: 10.1186/s13195-025-01787-7

Figure Lengend Snippet: PBM Improves Inflammation-induced TJs Damage in bEnd.3 Cells. A Schematic illustration of the Transwell BBB model and the optical parameters used for 808 nm laser irradiation. B FLU assay to evaluate the permeability of the monolayer bEnd.3 BBB model. C-E Immunofluorescence staining of bEnd.3 cells showing the expression of the TJs protein ZO-1 (red) and DAPI (blue), as well as LRP1 (red) with DAPI (blue), including quantitative analysis (Scale bar: 100 μm). F-I RT-PCR analysis of mRNA expression for TJs proteins Occludin, Claudin-5, ZO-1 and LRP1. J RT-PCR assessment of MMP-9 mRNA expression. K ELISA analysis of MMP-9 secretion in the supernatant of bEnd.3 cells. Data are presented as Mean ± SEM ( n = 3 per group). Comparisons indicate statistical significance relative to the Control group: * p < 0.05, ** p < 0.01, *** p < 0.001 and relative to the LPS group: # p < 0.05, ## p < 0.01, ### p < 0.001

Article Snippet: LRP1 Antibody , Wuhan Boster (BM4098) , 1:200.

Techniques: Irradiation, Permeability, Immunofluorescence, Staining, Expressing, Reverse Transcription Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Control

Journal: Alzheimer's Research & Therapy

Article Title: Photobiomodulation mitigates blood–brain barrier disruption in APP/PS1 mouse model of Alzheimer’s disease by activating the AMPK pathway

doi: 10.1186/s13195-025-01787-7

Figure Lengend Snippet: PBM Improves Inflammation-induced TJs Damage in bEnd.3 Cells by Activating AMPK. A Schematic diagram illustrating AMPK activation in bEnd.3 cells by PBM. B ELISA analysis of MMP-9 secretion in the supernatant of bEnd.3 cells following AICAR/Compound C treatment. C RT-PCR assessment of MMP-9 mRNA expression after treatment with AICAR/Compound C. D-E Immunofluorescence staining of the transport protein LRP1 (red) and DAPI (blue) in bEnd.3 cells following AICAR/Compound C treatment (Scale bar: 100 μm) with quantitative analysis. F–H RT-PCR analysis of Occludin, Claudin-5 and ZO-1 mRNA expression following AICAR/Compound C treatment. Data are presented as Mean ± SEM ( n = 3 per group). Comparisons indicate statistical significance relative to the Control + p-AMPK group: * p < 0.05, ** p < 0.01, *** p < 0.001 and relative to the Control + ko-AMPK group: # p < 0.05, ## p < 0.01, ### p < 0.001, relative to the p-AMPK group, ▲ p < 0.05, ▲▲ p < 0.01, ▲▲▲ p < 0.001

Article Snippet: LRP1 Antibody , Wuhan Boster (BM4098) , 1:200.

Techniques: Activation Assay, Enzyme-linked Immunosorbent Assay, Reverse Transcription Polymerase Chain Reaction, Expressing, Immunofluorescence, Staining, Control