Journal: BMC Biotechnology

Article Title: A new method for screening acute/chronic lymphocytic leukemia: dual-label time-resolved fluorescence immunoassay

doi: 10.1186/s12896-022-00758-2

Figure Lengend Snippet: The standard curves of S100A8 ( A ) and LRG1 ( B ). Standard curve determinations were carried out using linear regression and log–log regression. The Log function values of S100A8/LRG1 standards were plotted as X axis, and the Log function value of their fluorescence as the Y axis, performed a linear fit and draw a standard curve

Article Snippet: 59 clinical serum samples with acute/chronic LL were simultaneously measured by this TRFIA method, human S100A8 ELISA kit (Sigma-Aldrich, #RAB0730, USA) and LRG1 ELISA kit (CUSABIO, #CSB-E12962h, China).

Techniques: Fluorescence

Journal: BMC Biotechnology

Article Title: A new method for screening acute/chronic lymphocytic leukemia: dual-label time-resolved fluorescence immunoassay

doi: 10.1186/s12896-022-00758-2

Figure Lengend Snippet: Specificity of the dual-label TRFIA

Article Snippet: 59 clinical serum samples with acute/chronic LL were simultaneously measured by this TRFIA method, human S100A8 ELISA kit (Sigma-Aldrich, #RAB0730, USA) and LRG1 ELISA kit (CUSABIO, #CSB-E12962h, China).

Techniques: Concentration Assay

Journal: BMC Biotechnology

Article Title: A new method for screening acute/chronic lymphocytic leukemia: dual-label time-resolved fluorescence immunoassay

doi: 10.1186/s12896-022-00758-2

Figure Lengend Snippet: Accuracy and recovery of the dual-label TRFIA

Article Snippet: 59 clinical serum samples with acute/chronic LL were simultaneously measured by this TRFIA method, human S100A8 ELISA kit (Sigma-Aldrich, #RAB0730, USA) and LRG1 ELISA kit (CUSABIO, #CSB-E12962h, China).

Techniques: Inter Assay, Intra Assay

Journal: BMC Biotechnology

Article Title: A new method for screening acute/chronic lymphocytic leukemia: dual-label time-resolved fluorescence immunoassay

doi: 10.1186/s12896-022-00758-2

Figure Lengend Snippet: Bland–Altman plots of S100A8/LRG1 levels measured by TRFIA and ELISA kits

Article Snippet: 59 clinical serum samples with acute/chronic LL were simultaneously measured by this TRFIA method, human S100A8 ELISA kit (Sigma-Aldrich, #RAB0730, USA) and LRG1 ELISA kit (CUSABIO, #CSB-E12962h, China).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: BMC Biotechnology

Article Title: A new method for screening acute/chronic lymphocytic leukemia: dual-label time-resolved fluorescence immunoassay

doi: 10.1186/s12896-022-00758-2

Figure Lengend Snippet: Scheme of the dual-label TRFIA for S100A8 and LRG1 detection

Article Snippet: 59 clinical serum samples with acute/chronic LL were simultaneously measured by this TRFIA method, human S100A8 ELISA kit (Sigma-Aldrich, #RAB0730, USA) and LRG1 ELISA kit (CUSABIO, #CSB-E12962h, China).

Techniques:

Journal: Journal of neuroinflammation

Article Title: Single-cell RNA sequencing unveils Lrg1's role in cerebral ischemia‒reperfusion injury by modulating various cells.

doi: 10.1186/s12974-023-02941-4

Figure Lengend Snippet: Fig. 3 Single-cell transcriptomic data demonstrate that the impact of Lrg1 knockout on various cellular components in brain tissue of cerebral ischemia‒reperfusion mice. A A schematic representation of the experimental design employed in this study is depicted. B UMAP plots of 99,991 cells from 11 mice, including 14 cell types. C Heatmap of gene expression across different cell types. D Boxplot displaying cell purity for cell types. E Bar graph showing the number of differentially expressed genes for different cell types. F Bar graph showing the proportion of genes with unique changes in a single cell type among differentially upregulated or downregulated genes in MCAO/R + Lrg1−/− mice compared to MCAO/R + WT mice

Article Snippet: Lrg1 knockout mice Lrg1 knockout mice were obtained from Cyagen Biosciences Corporation (strain number: C57BL/6N-Lrg1em1cyagen, strain name: KOCMP-76905-Lrg1-B6N‒VA).

Techniques: Knock-Out, Gene Expression

Journal: Cancers

Article Title: Biomarker Panel for the Diagnosis of Pancreatic Ductal Adenocarcinoma

doi: 10.3390/cancers12061443

Figure Lengend Snippet: Comparison of 11 candidate markers concentration between PDAC samples and normal control samples. Among the 11 markers (ApoA1, CA125, CA19-9, CRP, CYFRA21.1, LRG1, CEA, ApoA2, TTR, B2M, and D.Dimer), 10 biomarkers except B2M showed statistical differences between PDAC and normal controls.

Article Snippet: LRG-1 was measured by an enzyme-linked immunosorbent assay (ELISA) using recombinant LRG1 protein and anti-Human LRG1 antibody (R&D Systems, Minneapolis, MN, USA).

Techniques: Comparison, Concentration Assay, Control

Journal: Experimental lung research

Article Title: Leucine rich α2 glycoprotein 1 derived from malignant pleural mesothelioma cells facilitates macrophage M2 phenotypes.

doi: 10.1080/01902148.2024.2380988

Figure Lengend Snippet: Figure 2. LRG1 was a potential candidate. Ten-fold concentrated supernatants from Met5A, Meso1, Meso2 or H2452 were subjected for 10% SDS-PAGE, and the gel was silver-stained and then analyzed by Mass Spectrometry. (A) Image of the silver-stained gel; (B) The Venn graph of Mass Spectrometry results; (C) ELISA analysis of LRG1 in the 10-fold concentrated supernatants of Met5A, Meso1, Meso2, and H2452 cells. Statistical significance was analyzed from three independent experiments by the two-tailed Student’s t test. ** and *** indicate P values <0.01 and 0.001, respectively.

Article Snippet: The culture media were then replaced by 0.5 mL of 9:1 mixture of fresh X-VIVO 15 and the 10-fold concentrated supernatants from Met5A, Meso1, Meso2 or H2452, or replaced by 0.5 mL fresh X-VIVO 15 containing 16 ng/mL recombinant human LRG1 (R&D systems, Minneapolis).

Techniques: SDS Page, Staining, Mass Spectrometry, Enzyme-linked Immunosorbent Assay, Two Tailed Test

Journal: Experimental lung research

Article Title: Leucine rich α2 glycoprotein 1 derived from malignant pleural mesothelioma cells facilitates macrophage M2 phenotypes.

doi: 10.1080/01902148.2024.2380988

Figure Lengend Snippet: Figure 3. LRG1 elicited dose-and time-dependent responses in THP-1 cells. THP-1 cells were stimulated with 50 ng/mL PMA for 12 h to allow initial activation and cell attachment. For observation of a dose-dependent response, the THP-1 cells were then cultured in fresh serum-free X-VIVO 15 containing different doses of recombinant human LRG1 for 24 h; For observation of a time-dependent response, the PMA-activated cells were cultured in fresh X-VIVO 15 containing 16 ng/mL of recombinant human LRG1 for different time. The culture media were collected for ELISA analysis of cytokines, and the cells were used for qPCR. (A) qRT-PCR analysis of the expression of IL-10, TGF-β and CD206 in THP-1 cells stimulated with different doses of LRG1 (0, 2, 4, 8, 16 and 32 ng/mL); (B) qRT-PCR analysis of the expression of IL-10, TGF-β and CD206 in THP-1 cells stimulated with 16 ng/mL LRG1 for different time (0, 12, 24 and 48 h); (C and D) ELISA detection of IL-10 and TGF-β in the superna tants of THP-1 cells stimulated with 16 ng/mL for 24 h. Statistical significance was analyzed from three independent experiments by One way ANOVA. * and **, versus the 0 ng/mL or 0 h groups, indicate P values <0.05 and 0.01, respectively.

Article Snippet: The culture media were then replaced by 0.5 mL of 9:1 mixture of fresh X-VIVO 15 and the 10-fold concentrated supernatants from Met5A, Meso1, Meso2 or H2452, or replaced by 0.5 mL fresh X-VIVO 15 containing 16 ng/mL recombinant human LRG1 (R&D systems, Minneapolis).

Techniques: Activation Assay, Cell Attachment Assay, Cell Culture, Recombinant, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Expressing

Journal: Experimental lung research

Article Title: Leucine rich α2 glycoprotein 1 derived from malignant pleural mesothelioma cells facilitates macrophage M2 phenotypes.

doi: 10.1080/01902148.2024.2380988

Figure Lengend Snippet: Figure 4. LRG1 enhanced M2 phenotypes via TGF-β receptor. THP-1 cells were activated with 50 ng/mL PMA and then stimulated with 16 ng/mL of recombinant human LRG1 in the presence or absence of 5 µm of the TGF-β receptor inhibitor SB-431542 for 24 h. The culture media were used for ELISA detection and the cells were used for western blot and immuno fluorescence. (A and B) ELISA detection of IL-10 and TGF-β in the supernatants of THP-1 cells; (C) Representative images of immunofluorescence staining of CD206-positive THP-1 cells; (D) Percentage of CD206-positive THP-1 cells; (E) western blot analysis of p-Smad2, Smad2, p-Smad3, and Smad3. GAPDH was used as an internal control; (F) The optical intensity of the bands analyzed using Image-Pro Plus. Statistical significance was analyzed from three independent experiments by the two-tailed Student’s t test. *, ** and ***indicate P values <0.05, 0.01 and 0.001; respectively, versus the control, while #, ## and ### indicate P values<0.05, 0.01 and 0.001; respectively, versus the LRG1 group.

Article Snippet: The culture media were then replaced by 0.5 mL of 9:1 mixture of fresh X-VIVO 15 and the 10-fold concentrated supernatants from Met5A, Meso1, Meso2 or H2452, or replaced by 0.5 mL fresh X-VIVO 15 containing 16 ng/mL recombinant human LRG1 (R&D systems, Minneapolis).

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Western Blot, Fluorescence, Immunofluorescence, Staining, Control, Two Tailed Test