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Image Search Results
Journal: MedComm
Article Title: Outer membrane vesicle contributes to the Pseudomonas aeruginosa resistance to antimicrobial peptides in the acidic airway of bronchiectasis patients
doi: 10.1002/mco2.70084
Figure Lengend Snippet: Lactate‐mediated acidification dampens the bactericidal activity of LL‐37 against P. aeruginosa . (A) Lactate concentrations of the sputa were collected from bronchiectasis (BE) patients and their relatives as controls in cohort 3. Induced sputa were collected when needed. The comparison was performed with an unpaired Student t ‐test. (B) Comparison of the sputum LL‐37 concentrations between BE patients with different microbiology test results in cohort 3. A comparison was performed with one‐way analysis of variance (ANOVA) with Welch's test. (C) The lactate concentrations in bronchoalveolar lavage fluid (BALF) from mice with or without P. aeruginosa chronic lung infection. A comparison was performed with an unpaired Student t ‐test. (D) The survivals of three clinical P. aeruginosa isolated from BE patients after the treatment with LL‐37 in vitro. Comparison between the untreated and LL‐37‐treated groups (120 µg/mL) was performed with an unpaired Student t ‐test. (E) Percentages of survived clinical P. aeruginosa isolate SHPHC2 post‐LL‐37 treatment in the conditions with various pH adjusted by lactate. One‐way ANOVA with Welch's test was used for statistical analysis in both LL37‐treated and untreated groups, p ‐values were indicated in the figure, and results with a p ‐value less than 0.05 were considered statistically significant. The error bars plotted indicate the standard error of the mean.
Article Snippet: The final concentrations of
Techniques: Activity Assay, Comparison, Infection, Isolation, In Vitro
Journal: MedComm
Article Title: Outer membrane vesicle contributes to the Pseudomonas aeruginosa resistance to antimicrobial peptides in the acidic airway of bronchiectasis patients
doi: 10.1002/mco2.70084
Figure Lengend Snippet: P. aeruginosa produces outer membrane vesicles (OMVs) to resist LL‐37‐mediated killing. (A) Quantification of OMVs produced by P. aeruginosa in the lactate‐mediated acidic environment by measuring FM 4‐64 fluorescence. One‐way analysis of variance (ANOVA) with Welch's test was used for statistical analysis. (B) Nanoparticle tracking analysis and transmission electron microscopy of OMVs isolated from P. aeruginosa overnight culture by ultracentrifugation. (C) Survival of P. aeruginosa PAO1 post‐LL‐37 treatment with the additions of OMVs of different dosages. The OMVs added were quantified by measuring the total proteins enwrapped. One‐way ANOVA with Welch's test was used for statistical analysis in both LL37‐treated and untreated groups and the p ‐value was shown. (D) The role of OMVs in the acidic condition‐induced strengthening of P. aeruginosa LL‐37 resistance was determined by adding Cl‐adimine to the bacteria‐killing system. The final concentration of Cl‐diamine was indicated on the diagram. The differences between groups were statistically analyzed by using one‐way ANOVA with Welch's test, with the p values of multiple comparisons indicated in the figures. Results with a p ‐value less than 0.05 were considered statistically significant. The error bars plotted indicate the standard error of the mean.
Article Snippet: The final concentrations of
Techniques: Membrane, Produced, Fluorescence, Transmission Assay, Electron Microscopy, Isolation, Bacteria, Concentration Assay