Journal: mBio

Article Title: A Novel Tool to Identify Bactericidal Compounds against Vulnerable Targets in Drug-Tolerant M. tuberculosis found in Caseum

doi: 10.1128/mbio.00598-23

Figure Lengend Snippet: Bactericidal activity of TB drugs against replicating Mtb cultures, native bacteria in caseum, and Mtb preadapted in iMtb-induced surrogate caseum for 6 weeks

Article Snippet: Rifampicin and levofloxacin were purchased from Gold Biotechnology (St. Louis, MO) and ChemImpex Intl Inc. (Wood Dale, IL), respectively.

Techniques: Activity Assay, Bacteria

Journal: Antibiotics

Article Title: Antibacterial Activity of the Novel Drug Gepotidacin against Stenotrophomonas maltophilia —An In Vitro and In Vivo Study

doi: 10.3390/antibiotics11020192

Figure Lengend Snippet: Determined minimum inhibitory concentrations (MICs) of gepotidacin, levofloxacin, moxifloxacin, and co-trimoxazole against S. maltophilia .

Article Snippet: Broth microdilution with gepotidacin, levofloxacin and moxifloxacin (MedChemExpress LLC, Monmouth, NJ, USA) was performed in concentrations between 0.03 and 16 mg/L.

Techniques:

Journal: The Analyst

Article Title: Towards therapeutic drug monitoring of antibiotic levels - analyzing the pharmacokinetics of levofloxacin using DUV-resonance Raman spectroscopy.

doi: 10.1039/d3an00402c

Figure Lengend Snippet: Fig. 1 Absorption spectrum of 0.1 mM aqueous solutions of levofloxa- cin (A). The applied laser excitation wavelength λexc. = 232 nm and the collected region of the Raman spectra are depicted as a vertical line and a shaded portion, respectively. Resonance Raman spectra (B) of water (1) and the highest concentration of levofloxacin used for calibration (50 µM, 2) with its marker band at about 1400 cm−1.

Article Snippet: The fluoroquinolone levofloxacin (levofloxacin hydrochloride, Santa Cruz Biotechnology Inc., Dallas, Texas USA, see Fig. 1) was used without further purification.

Techniques: Concentration Assay, Marker

Journal: The Analyst

Article Title: Towards therapeutic drug monitoring of antibiotic levels - analyzing the pharmacokinetics of levofloxacin using DUV-resonance Raman spectroscopy.

doi: 10.1039/d3an00402c

Figure Lengend Snippet: Fig. 2 The calibration curve using the intensity of the Raman marker band of levofloxacin at about 1400 cm−1 showed a good correlation with the concentration of the samples (ccal, A). For the prediction of the simulated concentrations via OLS regression, concentrations from 5 μM to 50 μM were used. The PLS regression model was optimized by calculating the minimum error via 100-fold cross-validation, resulting in an an optimum number of five components (big dot) for the prediction of the test data (ccal,test, B). With this value, a good relationship between the concentrations of the training (ccal,train) and test data (ccal,test) could be achieved, with the respective values depicted in blue and orange (C).

Article Snippet: The fluoroquinolone levofloxacin (levofloxacin hydrochloride, Santa Cruz Biotechnology Inc., Dallas, Texas USA, see Fig. 1) was used without further purification.

Techniques: Marker, Concentration Assay, Biomarker Discovery

Journal: The Analyst

Article Title: Towards therapeutic drug monitoring of antibiotic levels - analyzing the pharmacokinetics of levofloxacin using DUV-resonance Raman spectroscopy.

doi: 10.1039/d3an00402c

Figure Lengend Snippet: Fig. 4 Concentration profile after the intravenous (A) and oral (B) administration of 750 mg levofloxacin. Simulated concentrations (csim,int and csim,o, orange solid line) were compared with their predicted values (cpred,int and cpred,o) using either OLS (1) or PLS (2) regression. First-order (A) or Batman kinetics (B) were fitted (black solid line). Concentrations below the limit of detection (dashed horizontal line) were not satisfac- torily determined. Therefore, the resulting concentrations are too high for both models, but the PLS regression shows better overall agreement with the simulated data.

Article Snippet: The fluoroquinolone levofloxacin (levofloxacin hydrochloride, Santa Cruz Biotechnology Inc., Dallas, Texas USA, see Fig. 1) was used without further purification.

Techniques: Concentration Assay

Journal: Journal of Analytical Methods in Chemistry

Article Title: Spectrophotometric and Multivariate Calibration Techniques for Simultaneous Determination of Different Drugs in Pharmaceutical Formulations and Human Urine: Evaluation of Greenness Profile

doi: 10.1155/2020/8873003

Figure Lengend Snippet: Chemical structures of atenolol (a), paracetamol (b), hydrochlorothiazide (c), and levofloxacin (d).

Article Snippet: Levofloxacin hemihydrate (CAS no. 138199-71-0; 99% minimum purity) was purchased from Santa Cruz Biotechnology Inc. (Santa Cruz, Canada).

Techniques:

Journal: Journal of Analytical Methods in Chemistry

Article Title: Spectrophotometric and Multivariate Calibration Techniques for Simultaneous Determination of Different Drugs in Pharmaceutical Formulations and Human Urine: Evaluation of Greenness Profile

doi: 10.1155/2020/8873003

Figure Lengend Snippet: Zero-order absorption spectra of 10 μ g ml −1 atenolol (AT), 5 μ g ml −1 paracetamol (PR), 10 μ g ml −1 hydrochlorothiazide (HZ), and 5 μ g ml −1 levofloxacin (LV).

Article Snippet: Levofloxacin hemihydrate (CAS no. 138199-71-0; 99% minimum purity) was purchased from Santa Cruz Biotechnology Inc. (Santa Cruz, Canada).

Techniques: