Journal: The Journal of Neuroscience

Article Title: Spatially Heterogeneous Choroid Plexus Transcriptomes Encode Positional Identity and Contribute to Regional CSF Production

doi: 10.1523/JNEUROSCI.3081-14.2015

Figure Lengend Snippet: qRT-PCR measurement of genes detected by RNA-seq to be differentially expressed between tChP and hChP

Article Snippet: Lepr , Mm00440181_m1 , 1.000 , 0.473 ± 0.088 , 0.012.

Techniques:

Journal: bioRxiv

Article Title: Whole-body sorcin invalidation does not cause hypothalamic ER stress nor worsens obesity in C57BL/6 male mice fed a westernized diet

doi: 10.1101/2022.09.07.506981

Figure Lengend Snippet: (A) Representative Western (Immuno)blotting of pSTAT3 (Y705), STAT3 and Sorcin from whole protein lysate isolated from wild-type and sorcin null HEK293 cells expressing LepRb and stimulated with leptin (0-1 ng/mL) as described in methods. (B) Quantification of Western (Immuno)blotting from 3 independent experiments, showing the ratio between pSTAT3/STAT3 for cells stimulated with leptin (0-10 ng/mL); NT = non-transfected cells. Values are means ± SEM. Data were analysed for significance using 1-way ANOVA analysis with Tukey’s multiple comparisons test.

Article Snippet: HEK293 cells, seeded in 6-well plates, were transfected with 2 μg LepRb-HA plasmid, encoding full length clone DNA of Human leptin receptor, transcript variant 1 with C terminal HA tag (Sino-Biologicals, # HG10322-CY), using Xfect ™ Transfection Reagent (Takara Bio) according to manufacturer’s instructions.

Techniques: Western Blot, Isolation, Expressing, Transfection

Journal: PLoS ONE

Article Title: Acquired Alterations of Hypothalamic Gene Expression of Insulin and Leptin Receptors and Glucose Transporters in Prenatally High-Glucose Exposed Three-Week Old Chickens Do Not Coincide with Aberrant Promoter DNA Methylation

doi: 10.1371/journal.pone.0119213

Figure Lengend Snippet: Pyrosequencing assay information.

Article Snippet: TaqMan gene expression assays: INSR : Gg03330786_m1, LEPR : Gg03347016_m1, GLUT1 : Gg03367103_m1, GLUT3 : Gg03349364_m1 (all FAM-labeled), and BETA ACTIN : Gg03815934_s1, VIC-labeled, primer limited.

Techniques: Pyrosequencing Assay, Sequencing

Journal: PLoS ONE

Article Title: Acquired Alterations of Hypothalamic Gene Expression of Insulin and Leptin Receptors and Glucose Transporters in Prenatally High-Glucose Exposed Three-Week Old Chickens Do Not Coincide with Aberrant Promoter DNA Methylation

doi: 10.1371/journal.pone.0119213

Figure Lengend Snippet: Baseline characteristics, hypothalamic mRNA gene expression and overall promoter methylation in three-week-old chickens.

Article Snippet: TaqMan gene expression assays: INSR : Gg03330786_m1, LEPR : Gg03347016_m1, GLUT1 : Gg03367103_m1, GLUT3 : Gg03349364_m1 (all FAM-labeled), and BETA ACTIN : Gg03815934_s1, VIC-labeled, primer limited.

Techniques: Gene Expression, Methylation

Journal: PLoS ONE

Article Title: Acquired Alterations of Hypothalamic Gene Expression of Insulin and Leptin Receptors and Glucose Transporters in Prenatally High-Glucose Exposed Three-Week Old Chickens Do Not Coincide with Aberrant Promoter DNA Methylation

doi: 10.1371/journal.pone.0119213

Figure Lengend Snippet: Relative gene expression of insulin receptor ( INSR ), leptin receptor (LEPR ), and glucose transporters 1 and 3 ( GLUT1 , GLUT3 ), all normalized to BETA ACTIN . Data are given as means ± SEM, shown as percentage of prenatally NaCl-treated controls. * p <0.05 (Mann-Whitney U-test)

Article Snippet: TaqMan gene expression assays: INSR : Gg03330786_m1, LEPR : Gg03347016_m1, GLUT1 : Gg03367103_m1, GLUT3 : Gg03349364_m1 (all FAM-labeled), and BETA ACTIN : Gg03815934_s1, VIC-labeled, primer limited.

Techniques: Gene Expression, MANN-WHITNEY

Journal: Molecular Metabolism

Article Title: Hepatic leptin receptor expression can partially compensate for IL-6Rα deficiency in DEN-induced hepatocellular carcinoma

doi: 10.1016/j.molmet.2018.08.010

Figure Lengend Snippet: Hepatic Lepr expression is induced upon HFD feeding . (A) Western blot analysis of basal pSTAT3 level in whole liver lysates isolated from 7-month-old NCD- and HFD-fed mice (n = 9) and respective quantification (two-tailed unpaired t-test). pSTAT3/β-ACTIN ratio normalized to NCD expression. (B) qPCR analysis of Leprb mRNA expression in whole liver tissue isolated from 7-months-old NCD- or HFD-fed mice (probe spanning exon 17–18 of long Leprb ). Tbp was used as internal control, normalized to NCD (n = 6–7, two-tailed unpaired t-test). (C) Representative liver sections of NCD- and HFD-fed mice stained with ISH for Lepr mRNA and IHC for F4/80. Blue = DAPI, green = Lepr , red = F4/80. Scale bar = 50 μm. NCD = normal chow diet, HFD = high-fat diet. Data are means ± Min. and Max. p* ≤ 0 . 05 , p** ≤ 0 . 01 .

Article Snippet: The following TaqMan probes (Applied Biosystems) were used for gene expression assays: Il-6rα (Mm00439653_ml), Lepr (Mm01262069_m1), Lepr (Mm01265583_m1), Timp1 (Mm00441818_m1), Socs3 (Mm00545913_s1), Srebp1 (Mm00550338_m1), Srebp2 (Mm01306292_m1), Dgat1 (Mm00515643_m1), Dgat2 (Mm00499536_m1), Scd1 (Mm00772290_m1), Pparg (Mm00440945_m1), Fasn (Mm00662319_m1), Gck (Mm00439129_m1), Pepck (Mm00440636_m1), G6pc (Mm00839363_m1), Vegf (Mm00437304_m1), Egf (Mm01316968_m1), Egfr (Mm00433023_m1), Glut1 (Mm00441473_m1), Myc (Mm00487804_m1), Bcl2 (Mm00477631_m1), Mcl1 (Mm01257352_g1), Ccl2 (Mm00441242_m1), Ccl7 (Mm00443113_m1), Mmp9 (Mm00442991_m1), Stat3 (Mm00456961_m1), Tbp (Mm00446973_m1).

Techniques: Expressing, Western Blot, Isolation, Two Tailed Test, Control, Staining

Journal: Molecular Metabolism

Article Title: Hepatic leptin receptor expression can partially compensate for IL-6Rα deficiency in DEN-induced hepatocellular carcinoma

doi: 10.1016/j.molmet.2018.08.010

Figure Lengend Snippet: Hepatic LEPR deficiency further reduces DEN-induced HCC in IL-6Rα KO mice . (A) Serum AST and ALT levels after 6 and 8 months of age of Ctrl (n = 4–10), LepR L−KO (n = 8–10), IL-6Rα KO (n = 5–9), and D-KO (n = 8–12) mice fed a NCD (ordinary two-way ANOVA). (B) Liver weight after 8 months of age of NCD-fed Ctrl, LepR L−KO , IL-6Rα KO , and D-KO (n = 9–10, ordinary one-way ANOVA). (C) Tumor burden after 8 months of age of NCD-fed Ctrl, LepR L−KO , IL-6Rα KO , and D-KO (n = 10, multiple t-test with correction for multiple comparison, Holm-Sidak method). (D) Representative pictures of H&E stainings of non-tumor liver tissue from NCD-fed Ctrl, LepR L−KO , IL-6Rα KO , and D-KO (n = 3). Scale bar = 100 μm. (E) Fold change of activated caspase 3 in liver lysates isolated from 8-months-old DEN injected NCD-fed mutant mice, normalized to Ctrl (n = 10, ordinary one-way ANOVA). (F) Representative pictures of Ki67 IHC (red) in livers of DEN injected NCD-fed Ctrl, LepR L−KO , IL-6Rα KO , and D-KO mice and respective quantification (n = 5–6, ordinary one-way ANOVA). Data are presented as percentage of Ki67 positive cells normalized to DAPI (blue) positive nuclei. Scale bar = 50 μm. AST = aspartate aminotransferase, ALT = alanine aminotransferase, NCD = normal chow diet. Data are means ± Min. and Max. p* ≤ 0 . 05 , p** ≤ 0 . 01 , p*** ≤ 0 . 001 , p**** ≤ 0 . 0001 .

Article Snippet: The following TaqMan probes (Applied Biosystems) were used for gene expression assays: Il-6rα (Mm00439653_ml), Lepr (Mm01262069_m1), Lepr (Mm01265583_m1), Timp1 (Mm00441818_m1), Socs3 (Mm00545913_s1), Srebp1 (Mm00550338_m1), Srebp2 (Mm01306292_m1), Dgat1 (Mm00515643_m1), Dgat2 (Mm00499536_m1), Scd1 (Mm00772290_m1), Pparg (Mm00440945_m1), Fasn (Mm00662319_m1), Gck (Mm00439129_m1), Pepck (Mm00440636_m1), G6pc (Mm00839363_m1), Vegf (Mm00437304_m1), Egf (Mm01316968_m1), Egfr (Mm00433023_m1), Glut1 (Mm00441473_m1), Myc (Mm00487804_m1), Bcl2 (Mm00477631_m1), Mcl1 (Mm01257352_g1), Ccl2 (Mm00441242_m1), Ccl7 (Mm00443113_m1), Mmp9 (Mm00442991_m1), Stat3 (Mm00456961_m1), Tbp (Mm00446973_m1).

Techniques: Comparison, Isolation, Injection, Mutagenesis

Journal: Molecular Metabolism

Article Title: Hepatic leptin receptor expression can partially compensate for IL-6Rα deficiency in DEN-induced hepatocellular carcinoma

doi: 10.1016/j.molmet.2018.08.010

Figure Lengend Snippet: Hepatic LEPR and whole body IL-6Rα deficiency display only marginal effects on whole body metabolism in DEN-induced HCC . (A) Body weight curve of Ctrl, LepR L−KO , IL-6Rα KO , and D-KO (n = 10) mice fed a NCD. (B) Absolute epididymal WAT weight of NCD-fed Ctrl, LepR L−KO , IL-6Rα KO , and D-KO with 8 months (n = 10, ordinary one-way ANOVA). (C) Serum leptin levels from NCD-fed 6-month-old mice (n = 6–10, ordinary one-way ANOVA). Insulin tolerance determined at (D) 11 weeks of age of Ctrl, LepR L−KO , IL-6Rα KO , and D-KO (n = 10) and (E) 24 weeks of age of Ctrl (n = 10), LepR L−KO (n = 10), IL-6Rα KO (n = 5), and D-KO (n = 10) (ordinary two-way ANOVA) mice fed a NCD. Values are displayed as % of initial basal blood glucose. Glucose tolerance determined at (F) 12 weeks of age and (G) 25 weeks of age of Ctrl (n = 10), LepR L−KO (n = 10), IL-6Rα KO (n = 5), and D-KO (n = 10) (ordinary two-way ANOVA) mice fed a NCD. (H) Serum insulin levels from NCD-fed 6-months-old mice (n = 6–10, ordinary one-way ANOVA). WAT = white adipose tissue, NCD = normal chow diet. n.s. = not significant. p* ≤ 0 . 05 . Data are means ± Min. and Max (B, C, H) or ± SEM (A, D, E, F, G).

Article Snippet: The following TaqMan probes (Applied Biosystems) were used for gene expression assays: Il-6rα (Mm00439653_ml), Lepr (Mm01262069_m1), Lepr (Mm01265583_m1), Timp1 (Mm00441818_m1), Socs3 (Mm00545913_s1), Srebp1 (Mm00550338_m1), Srebp2 (Mm01306292_m1), Dgat1 (Mm00515643_m1), Dgat2 (Mm00499536_m1), Scd1 (Mm00772290_m1), Pparg (Mm00440945_m1), Fasn (Mm00662319_m1), Gck (Mm00439129_m1), Pepck (Mm00440636_m1), G6pc (Mm00839363_m1), Vegf (Mm00437304_m1), Egf (Mm01316968_m1), Egfr (Mm00433023_m1), Glut1 (Mm00441473_m1), Myc (Mm00487804_m1), Bcl2 (Mm00477631_m1), Mcl1 (Mm01257352_g1), Ccl2 (Mm00441242_m1), Ccl7 (Mm00443113_m1), Mmp9 (Mm00442991_m1), Stat3 (Mm00456961_m1), Tbp (Mm00446973_m1).

Techniques:

Journal: Molecular Metabolism

Article Title: Hepatic leptin receptor expression can partially compensate for IL-6Rα deficiency in DEN-induced hepatocellular carcinoma

doi: 10.1016/j.molmet.2018.08.010

Figure Lengend Snippet: IL-6Rα deficiency increases hepatic Lepr expression in DEN-induced HCC livers . qPCR analysis of (A) Il6rα , (B) Socs3 , (C) Timp1 , and (D) Leprb (probe spanning exon 18–19 of long Leprb ) mRNA in whole liver tissue isolated from tumor bearing (DEN-induced HCC) NCD- and HFD-fed IL-6Rα KO and control mice, normalized to Ctrl NCD (n = 3–8, ordinary two-way ANOVA). (E) Serum leptin levels from 8-months-old IL-6Rα KO and control mice in DEN-induced HCC upon NCD and HFD feeding (n = 11–12, ordinary two-way ANOVA). (F) Corresponding body weights of Ctrl and IL-6Rα KO mice at 8 months (n = 11–12, ordinary two-way ANOVA). (G) Schematic representation of mouse mutants used in this study. (H, I) Western blot analyses of pSTAT3 in hepatocytes isolated from indicated mouse mutants stimulated with either (H) 50 ng/ml IL-6 or (I) 1 μg/ml leptin for 15, 30 or 60 min. (J) Time beam of DEN experiments and metabolic phenotyping. HCC = hepatocellular carcinoma, DEN = diethylnitrosamine, NCD = normal chow diet, HFD = high-fat diet. p* ≤ 0 . 05 , p** ≤ 0 . 01 , p*** ≤ 0 . 001 , p**** ≤ 0 . 0001 . Data are means ± Min. and Max.

Article Snippet: The following TaqMan probes (Applied Biosystems) were used for gene expression assays: Il-6rα (Mm00439653_ml), Lepr (Mm01262069_m1), Lepr (Mm01265583_m1), Timp1 (Mm00441818_m1), Socs3 (Mm00545913_s1), Srebp1 (Mm00550338_m1), Srebp2 (Mm01306292_m1), Dgat1 (Mm00515643_m1), Dgat2 (Mm00499536_m1), Scd1 (Mm00772290_m1), Pparg (Mm00440945_m1), Fasn (Mm00662319_m1), Gck (Mm00439129_m1), Pepck (Mm00440636_m1), G6pc (Mm00839363_m1), Vegf (Mm00437304_m1), Egf (Mm01316968_m1), Egfr (Mm00433023_m1), Glut1 (Mm00441473_m1), Myc (Mm00487804_m1), Bcl2 (Mm00477631_m1), Mcl1 (Mm01257352_g1), Ccl2 (Mm00441242_m1), Ccl7 (Mm00443113_m1), Mmp9 (Mm00442991_m1), Stat3 (Mm00456961_m1), Tbp (Mm00446973_m1).

Techniques: Expressing, Isolation, Control, Western Blot