lentivirus transduction Search Results


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Shanghai GenePharma lentivirus transfection reagent
Inhibition of tumour growth and angiogenesis of PC in vivo are induced by miR‐27a silencing. A, Tumorigenesis of PANC‐1 cells following the injection of <t>lentivirus</t> with miR‐27a mimic and miR‐27a inhibitor. B and C, Tumour volume and weight after alteration of miR‐27a. D, The expression of miR‐27a in serum exosomes of nude mice after alteration of miR‐27a. E and F, Protein levels of BTG2 and VEGF in tumour tissues of nude mice after alteration of miR‐27a. G and H, MVD in tumour tissues of nude mice after alteration of miR‐27a (400×). * P < .05 vs nude mice injected with lentivirus with miR‐27a mimic‐NC or miR‐27a inhibitor‐NC. The above data are measurement data and described as mean ± standard deviation. The data of Panel B are analysed by repeated measurement ANOVA; data of other panels are analysed by one‐way analysis of variance. n = 12. The experiment was repeated 3 times independently. miR‐27a, microRNA‐27a; BTG2, B‐cell translocation gene 2; PC, pancreatic cancer; NC, negative control; MVD, microvessel density; VEGF, vascular endothelial growth factor
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Inhibition of tumour growth and angiogenesis of PC in vivo are induced by miR‐27a silencing. A, Tumorigenesis of PANC‐1 cells following the injection of <t>lentivirus</t> with miR‐27a mimic and miR‐27a inhibitor. B and C, Tumour volume and weight after alteration of miR‐27a. D, The expression of miR‐27a in serum exosomes of nude mice after alteration of miR‐27a. E and F, Protein levels of BTG2 and VEGF in tumour tissues of nude mice after alteration of miR‐27a. G and H, MVD in tumour tissues of nude mice after alteration of miR‐27a (400×). * P < .05 vs nude mice injected with lentivirus with miR‐27a mimic‐NC or miR‐27a inhibitor‐NC. The above data are measurement data and described as mean ± standard deviation. The data of Panel B are analysed by repeated measurement ANOVA; data of other panels are analysed by one‐way analysis of variance. n = 12. The experiment was repeated 3 times independently. miR‐27a, microRNA‐27a; BTG2, B‐cell translocation gene 2; PC, pancreatic cancer; NC, negative control; MVD, microvessel density; VEGF, vascular endothelial growth factor
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Inhibition of tumour growth and angiogenesis of PC in vivo are induced by miR‐27a silencing. A, Tumorigenesis of PANC‐1 cells following the injection of <t>lentivirus</t> with miR‐27a mimic and miR‐27a inhibitor. B and C, Tumour volume and weight after alteration of miR‐27a. D, The expression of miR‐27a in serum exosomes of nude mice after alteration of miR‐27a. E and F, Protein levels of BTG2 and VEGF in tumour tissues of nude mice after alteration of miR‐27a. G and H, MVD in tumour tissues of nude mice after alteration of miR‐27a (400×). * P < .05 vs nude mice injected with lentivirus with miR‐27a mimic‐NC or miR‐27a inhibitor‐NC. The above data are measurement data and described as mean ± standard deviation. The data of Panel B are analysed by repeated measurement ANOVA; data of other panels are analysed by one‐way analysis of variance. n = 12. The experiment was repeated 3 times independently. miR‐27a, microRNA‐27a; BTG2, B‐cell translocation gene 2; PC, pancreatic cancer; NC, negative control; MVD, microvessel density; VEGF, vascular endothelial growth factor
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Promega viral transduction lentivirus
Inhibition of tumour growth and angiogenesis of PC in vivo are induced by miR‐27a silencing. A, Tumorigenesis of PANC‐1 cells following the injection of <t>lentivirus</t> with miR‐27a mimic and miR‐27a inhibitor. B and C, Tumour volume and weight after alteration of miR‐27a. D, The expression of miR‐27a in serum exosomes of nude mice after alteration of miR‐27a. E and F, Protein levels of BTG2 and VEGF in tumour tissues of nude mice after alteration of miR‐27a. G and H, MVD in tumour tissues of nude mice after alteration of miR‐27a (400×). * P < .05 vs nude mice injected with lentivirus with miR‐27a mimic‐NC or miR‐27a inhibitor‐NC. The above data are measurement data and described as mean ± standard deviation. The data of Panel B are analysed by repeated measurement ANOVA; data of other panels are analysed by one‐way analysis of variance. n = 12. The experiment was repeated 3 times independently. miR‐27a, microRNA‐27a; BTG2, B‐cell translocation gene 2; PC, pancreatic cancer; NC, negative control; MVD, microvessel density; VEGF, vascular endothelial growth factor
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Purigo Biotech Inc lentivirus-mediated transduction using synthetic short hairpin rna (shrna) oligonucleotide
Inhibition of tumour growth and angiogenesis of PC in vivo are induced by miR‐27a silencing. A, Tumorigenesis of PANC‐1 cells following the injection of <t>lentivirus</t> with miR‐27a mimic and miR‐27a inhibitor. B and C, Tumour volume and weight after alteration of miR‐27a. D, The expression of miR‐27a in serum exosomes of nude mice after alteration of miR‐27a. E and F, Protein levels of BTG2 and VEGF in tumour tissues of nude mice after alteration of miR‐27a. G and H, MVD in tumour tissues of nude mice after alteration of miR‐27a (400×). * P < .05 vs nude mice injected with lentivirus with miR‐27a mimic‐NC or miR‐27a inhibitor‐NC. The above data are measurement data and described as mean ± standard deviation. The data of Panel B are analysed by repeated measurement ANOVA; data of other panels are analysed by one‐way analysis of variance. n = 12. The experiment was repeated 3 times independently. miR‐27a, microRNA‐27a; BTG2, B‐cell translocation gene 2; PC, pancreatic cancer; NC, negative control; MVD, microvessel density; VEGF, vascular endothelial growth factor
Lentivirus Mediated Transduction Using Synthetic Short Hairpin Rna (Shrna) Oligonucleotide, supplied by Purigo Biotech Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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VectorBuilder GmbH lentivirus transduction demo results
Inhibition of tumour growth and angiogenesis of PC in vivo are induced by miR‐27a silencing. A, Tumorigenesis of PANC‐1 cells following the injection of <t>lentivirus</t> with miR‐27a mimic and miR‐27a inhibitor. B and C, Tumour volume and weight after alteration of miR‐27a. D, The expression of miR‐27a in serum exosomes of nude mice after alteration of miR‐27a. E and F, Protein levels of BTG2 and VEGF in tumour tissues of nude mice after alteration of miR‐27a. G and H, MVD in tumour tissues of nude mice after alteration of miR‐27a (400×). * P < .05 vs nude mice injected with lentivirus with miR‐27a mimic‐NC or miR‐27a inhibitor‐NC. The above data are measurement data and described as mean ± standard deviation. The data of Panel B are analysed by repeated measurement ANOVA; data of other panels are analysed by one‐way analysis of variance. n = 12. The experiment was repeated 3 times independently. miR‐27a, microRNA‐27a; BTG2, B‐cell translocation gene 2; PC, pancreatic cancer; NC, negative control; MVD, microvessel density; VEGF, vascular endothelial growth factor
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Shanghai GenePharma lentivirus (lv) products for transduction of sherk1/2, shsox9 and shctr
Inhibition of tumour growth and angiogenesis of PC in vivo are induced by miR‐27a silencing. A, Tumorigenesis of PANC‐1 cells following the injection of <t>lentivirus</t> with miR‐27a mimic and miR‐27a inhibitor. B and C, Tumour volume and weight after alteration of miR‐27a. D, The expression of miR‐27a in serum exosomes of nude mice after alteration of miR‐27a. E and F, Protein levels of BTG2 and VEGF in tumour tissues of nude mice after alteration of miR‐27a. G and H, MVD in tumour tissues of nude mice after alteration of miR‐27a (400×). * P < .05 vs nude mice injected with lentivirus with miR‐27a mimic‐NC or miR‐27a inhibitor‐NC. The above data are measurement data and described as mean ± standard deviation. The data of Panel B are analysed by repeated measurement ANOVA; data of other panels are analysed by one‐way analysis of variance. n = 12. The experiment was repeated 3 times independently. miR‐27a, microRNA‐27a; BTG2, B‐cell translocation gene 2; PC, pancreatic cancer; NC, negative control; MVD, microvessel density; VEGF, vascular endothelial growth factor
Lentivirus (Lv) Products For Transduction Of Sherk1/2, Shsox9 And Shctr, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
VectorBuilder GmbH lentivirus transduction
Inhibition of tumour growth and angiogenesis of PC in vivo are induced by miR‐27a silencing. A, Tumorigenesis of PANC‐1 cells following the injection of <t>lentivirus</t> with miR‐27a mimic and miR‐27a inhibitor. B and C, Tumour volume and weight after alteration of miR‐27a. D, The expression of miR‐27a in serum exosomes of nude mice after alteration of miR‐27a. E and F, Protein levels of BTG2 and VEGF in tumour tissues of nude mice after alteration of miR‐27a. G and H, MVD in tumour tissues of nude mice after alteration of miR‐27a (400×). * P < .05 vs nude mice injected with lentivirus with miR‐27a mimic‐NC or miR‐27a inhibitor‐NC. The above data are measurement data and described as mean ± standard deviation. The data of Panel B are analysed by repeated measurement ANOVA; data of other panels are analysed by one‐way analysis of variance. n = 12. The experiment was repeated 3 times independently. miR‐27a, microRNA‐27a; BTG2, B‐cell translocation gene 2; PC, pancreatic cancer; NC, negative control; MVD, microvessel density; VEGF, vascular endothelial growth factor
Lentivirus Transduction, supplied by VectorBuilder GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Shanghai GenePharma shrna lentivirus transduction particles designed to target b3gat3
Inhibition of tumour growth and angiogenesis of PC in vivo are induced by miR‐27a silencing. A, Tumorigenesis of PANC‐1 cells following the injection of <t>lentivirus</t> with miR‐27a mimic and miR‐27a inhibitor. B and C, Tumour volume and weight after alteration of miR‐27a. D, The expression of miR‐27a in serum exosomes of nude mice after alteration of miR‐27a. E and F, Protein levels of BTG2 and VEGF in tumour tissues of nude mice after alteration of miR‐27a. G and H, MVD in tumour tissues of nude mice after alteration of miR‐27a (400×). * P < .05 vs nude mice injected with lentivirus with miR‐27a mimic‐NC or miR‐27a inhibitor‐NC. The above data are measurement data and described as mean ± standard deviation. The data of Panel B are analysed by repeated measurement ANOVA; data of other panels are analysed by one‐way analysis of variance. n = 12. The experiment was repeated 3 times independently. miR‐27a, microRNA‐27a; BTG2, B‐cell translocation gene 2; PC, pancreatic cancer; NC, negative control; MVD, microvessel density; VEGF, vascular endothelial growth factor
Shrna Lentivirus Transduction Particles Designed To Target B3gat3, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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86
Shanghai Genechem Ltd transduction lentivirus expressing mtkeima red
Inhibition of tumour growth and angiogenesis of PC in vivo are induced by miR‐27a silencing. A, Tumorigenesis of PANC‐1 cells following the injection of <t>lentivirus</t> with miR‐27a mimic and miR‐27a inhibitor. B and C, Tumour volume and weight after alteration of miR‐27a. D, The expression of miR‐27a in serum exosomes of nude mice after alteration of miR‐27a. E and F, Protein levels of BTG2 and VEGF in tumour tissues of nude mice after alteration of miR‐27a. G and H, MVD in tumour tissues of nude mice after alteration of miR‐27a (400×). * P < .05 vs nude mice injected with lentivirus with miR‐27a mimic‐NC or miR‐27a inhibitor‐NC. The above data are measurement data and described as mean ± standard deviation. The data of Panel B are analysed by repeated measurement ANOVA; data of other panels are analysed by one‐way analysis of variance. n = 12. The experiment was repeated 3 times independently. miR‐27a, microRNA‐27a; BTG2, B‐cell translocation gene 2; PC, pancreatic cancer; NC, negative control; MVD, microvessel density; VEGF, vascular endothelial growth factor
Transduction Lentivirus Expressing Mtkeima Red, supplied by Shanghai Genechem Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Inhibition of tumour growth and angiogenesis of PC in vivo are induced by miR‐27a silencing. A, Tumorigenesis of PANC‐1 cells following the injection of lentivirus with miR‐27a mimic and miR‐27a inhibitor. B and C, Tumour volume and weight after alteration of miR‐27a. D, The expression of miR‐27a in serum exosomes of nude mice after alteration of miR‐27a. E and F, Protein levels of BTG2 and VEGF in tumour tissues of nude mice after alteration of miR‐27a. G and H, MVD in tumour tissues of nude mice after alteration of miR‐27a (400×). * P < .05 vs nude mice injected with lentivirus with miR‐27a mimic‐NC or miR‐27a inhibitor‐NC. The above data are measurement data and described as mean ± standard deviation. The data of Panel B are analysed by repeated measurement ANOVA; data of other panels are analysed by one‐way analysis of variance. n = 12. The experiment was repeated 3 times independently. miR‐27a, microRNA‐27a; BTG2, B‐cell translocation gene 2; PC, pancreatic cancer; NC, negative control; MVD, microvessel density; VEGF, vascular endothelial growth factor

Journal: Journal of Cellular and Molecular Medicine

Article Title: Pancreatic cancer cell–derived exosomal microRNA‐27a promotes angiogenesis of human microvascular endothelial cells in pancreatic cancer via BTG2

doi: 10.1111/jcmm.14766

Figure Lengend Snippet: Inhibition of tumour growth and angiogenesis of PC in vivo are induced by miR‐27a silencing. A, Tumorigenesis of PANC‐1 cells following the injection of lentivirus with miR‐27a mimic and miR‐27a inhibitor. B and C, Tumour volume and weight after alteration of miR‐27a. D, The expression of miR‐27a in serum exosomes of nude mice after alteration of miR‐27a. E and F, Protein levels of BTG2 and VEGF in tumour tissues of nude mice after alteration of miR‐27a. G and H, MVD in tumour tissues of nude mice after alteration of miR‐27a (400×). * P < .05 vs nude mice injected with lentivirus with miR‐27a mimic‐NC or miR‐27a inhibitor‐NC. The above data are measurement data and described as mean ± standard deviation. The data of Panel B are analysed by repeated measurement ANOVA; data of other panels are analysed by one‐way analysis of variance. n = 12. The experiment was repeated 3 times independently. miR‐27a, microRNA‐27a; BTG2, B‐cell translocation gene 2; PC, pancreatic cancer; NC, negative control; MVD, microvessel density; VEGF, vascular endothelial growth factor

Article Snippet: All the lentivirus transfection reagents were purchased from Shanghai GenePharma Co Ltd. After 1 week, the pancreas of the female mice was inoculated with stably expressed PANC‐1 cells at the logarithmic growth phase by intraperitoneal injection with 200 μL cell suspension (15 mice per group).

Techniques: Inhibition, In Vivo, Injection, Expressing, Standard Deviation, Translocation Assay, Negative Control