Journal: Diabetologia

Article Title: The type 2 diabetes presumed causal variant within TCF7L2 resides in an element that controls the expression of ACSL5.

doi: 10.1007/s00125-016-4077-2

Figure Lengend Snippet: Fig. 1 Expression analysis of ACSL5 and TCF7L2 following gene editing of the genomic region harbouring rs7903146. Relative levels of individual gene expression in the control and targeted cells, determined by quantitative PCR with GADPH normalisation. (a) ACSL5 and (b) TCF7L2 expression in the HCT116 control and targeted cells. (c) ACSL5 expression in the NCM460 control and targeted cells. (d) TCF7L2 expression in the NCM460 control and targeted cells. CTL, control; del, deletion. Values represent the mean of three experiments ± SDs. *p < 0.05, ***p < 0.001

Article Snippet: Targeting the genomic region encompassing rs7903146 in HCT116 cells (1.4 kb deletion) A bicistronic vector pX330, expressing both a human codonoptimised Streptococcus pyogenes clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein-9 nuclease (SpCas9) and a chimeric guide RNA, was purchased from Addgene (Plasmid 42230; Cambridge, MA, USA) [14].

Techniques: Expressing, Gene Expression, Control, Real-time Polymerase Chain Reaction