Journal: Neuroscience Bulletin

Article Title: A Two-Step GRIN Lens Coating for In Vivo Brain Imaging

doi: 10.1007/s12264-019-00356-x

Figure Lengend Snippet: Images of primary rat hippocampal neurons after 15 days in culture with coated and uncoated lenses. A Parylene-C-coated 1-mm diameter Go!Foton lenses. There was no parylene-C coating on the top end of the GRIN lens (arrow:coating interface). B Hippocampal neurons cultured with a coated lens maintained normal morphology. Left, coated lens; middle, neurons on the surface of lens (arrows); right, neurons near the lens. C Neurons cultured with an uncoated lens were characterized by the complete loss of neurites and changes in the cytoplasm. Left, uncoated lens; middle, neurons on the surface of lens (arrows); right, neurons near the lens. Scale bars, 100 μm.

Article Snippet: To determine whether the Go!Foton GRIN lens leached thallium ion into the solution, we soaked 8 uncoated lenses in 5 mL PBS for 20 days at room temperature.

Techniques: Cell Culture

Journal: Neuroscience Bulletin

Article Title: A Two-Step GRIN Lens Coating for In Vivo Brain Imaging

doi: 10.1007/s12264-019-00356-x

Figure Lengend Snippet: Two steps of coating enable successful in vivo imaging using Go!Foton lenses. A Neither active neurons nor blood vessels were observed under a Go!Foton lens without coating. B Active neurons (arrowhead) and blood clots (arrow) were observed under a GRIN lens coat with parylene-C alone. C Both active neurons (arrowhead) and blood vessels (arrow) were observed under GRIN lenses coated with both parylene-C and 50 μg/mL fibronectin. D Both active neurons (arrowhead) and blood vessels (arrow) were observed under a GrinTech lens. Scale bar, 100 μm.

Article Snippet: To determine whether the Go!Foton GRIN lens leached thallium ion into the solution, we soaked 8 uncoated lenses in 5 mL PBS for 20 days at room temperature.

Techniques: In Vivo Imaging

Journal: Neuroscience Bulletin

Article Title: A Two-Step GRIN Lens Coating for In Vivo Brain Imaging

doi: 10.1007/s12264-019-00356-x

Figure Lengend Snippet: Comparison of imaging performance. A–C Representative standard deviation projection images from miniScope recording via GRIN lenses coated with parylene-C and fibronectin at 100 μg/mL (Group 1) (A), 50 μg/mL (Group 2) (B), and 25 μg/mL (Group 3) (C). D Representative standard deviation projection image from miniScope recording via a GrinTech GRIN lens (Group 4). E Average ∆F/F of all the neurons from each group. Group 1: 2.46% ± 0.06%, 96 neurons from two mice; Group 2: 4.50% ± 0.13%, 224 neurons from two mice; Group 3: 5.55% ± 0.34%, 100 neurons from two mice; Group 4: 4.85% ± 0.14%, 366 neurons from three mice. ****P < 0.0001, one-way ANOVA and Tukey’s post hoc test. Histogram bars represent the mean value for all neurons, with error bars representing SEM. Scale bar, 100 μm.

Article Snippet: To determine whether the Go!Foton GRIN lens leached thallium ion into the solution, we soaked 8 uncoated lenses in 5 mL PBS for 20 days at room temperature.

Techniques: Comparison, Imaging, Standard Deviation

Journal: bioRxiv

Article Title: Alternative splicing links histone modifications to stem cell fate decision

doi: 10.1101/181875

Figure Lengend Snippet: (A) Genome browser view shows the AS event and H3K36me3 signals of PBX1 upon hESC differentiation. The green horizontal bars below the ChIP-seq tracks indicate the narrow peaks called by MACS2. (B) The inclusion level for exon 7 of PBX1 is significantly correlated to the H3K36me3 signals over this exon across cell lineages. (C) The sequence difference of three protein isoforms of PBX1 and the main functional domains. (D) The relative expressions of PBX1a and PBX1b in 56 cells/tissues, representing the differential expressions of two isoforms in three groups based on their developmental states. (E) The expression levels of NANOG and OCT4 genes are negatively correlated with the expression of PBX1b. (F) The expression levels of PSIP1 and SRSF1 show significant positive correlations with the expression level of PBX1a. Also, see Figure S9-S10 .

Article Snippet: The SRSF1-bound PSIP1 protein (also known as LEDGF) level was determined with the PSIP1 antibody (Human LEDGF Antibody, R&D systems) using Western blot as described above.

Techniques: ChIP-sequencing, Sequencing, Functional Assay, Expressing

Journal: bioRxiv

Article Title: Alternative splicing links histone modifications to stem cell fate decision

doi: 10.1101/181875

Figure Lengend Snippet: (A) qRT-PCR and western blot show the expression levels of Yamanaka factors in H1, MSC and IMR90 cells. Whiskers denote the standard deviations of three replicates. (B) RT-PCR and western blot show the isoform switches between PBX1a and PBX1b from H1 cells to differentiated cells. (C) i . ChIP-PCR shows the differential binding of PBX1b to NANOG promoter in H1 cells and differentiated cells; ii . ChIP-PCR shows the reduced H3K36me3 signal in differentiated cells; iii. ChIP-PCR shows the differential recruitment of PSIP1 to the exon 7 of PBX1. (D) RIP-PCR show the differential recruitment of SRSF1 around the exon 7 of PBX1. (E) Co-IP shows the overall physical interaction between PSIP1 and SRSF1 in all studied cell types. (F) The mechanism by which H3K36me3 is linked to cell fate decision by regulating the isoform switch of PBX1, which functions upstream of the pluripotency regulatory network. Also, see Figures S9-S10 .

Article Snippet: The SRSF1-bound PSIP1 protein (also known as LEDGF) level was determined with the PSIP1 antibody (Human LEDGF Antibody, R&D systems) using Western blot as described above.

Techniques: Quantitative RT-PCR, Western Blot, Expressing, Reverse Transcription Polymerase Chain Reaction, Binding Assay, Co-Immunoprecipitation Assay