Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

Article Title: Transgenic Overexpression of Tissue‐Nonspecific Alkaline Phosphatase ( TNAP ) in Vascular Endothelium Results in Generalized Arterial Calcification

doi: 10.1161/JAHA.115.002499

Figure Lengend Snippet: Expression of eTNAP. A, AP activity staining of the aorta, heart, lung, kidney, liver, small intestine, spleen, thymus, pancreas, and mesenteric LN from WT and eTNAP mice aged 8 weeks. B, Colocalization of AP activity with endothelial‐specific lectin ( IB 4) staining in the consecutive sections of myocardium counterstained with DAPI ( DNA ). C, Whole‐body x‐ray images of WT and eTNAP mice aged 8 weeks. AP indicates alkaline phosphatase; eTNAP, endothelial tissue‐nonspecific alkaline phosphatase; IB4, isolectin B4; LN, lymph node; WT, wild type.

Article Snippet: ECs were detected with FITC‐labeled isolectin B4 (IB4) from Bandeiraea simplicifolia (item FL1201; Vector Laboratories).

Techniques: Expressing, Activity Assay, Staining

Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

Article Title: Transgenic Overexpression of Tissue‐Nonspecific Alkaline Phosphatase ( TNAP ) in Vascular Endothelium Results in Generalized Arterial Calcification

doi: 10.1161/JAHA.115.002499

Figure Lengend Snippet: Structural characteristics of arterial lesions in eTNAP mice. A, Detection of Alizarin Red–positive lesions in flat‐mounted preparations of the aortas from eTNAP mice aged 8 weeks. B, Calcification in coronary artery sinus (top) and coronary arteries (bottom) in eTNAP mice aged 8 weeks visualized by Alizarin Red staining. C, Subendothelial localization of coronary lesions (highlighted by arrows) in eTNAP mice aged 13 weeks detected by AP and endothelial‐specific ( IB 4) staining in consecutive sections counterstained with DAPI ( DNA ). D, von Kossa–positive calcium deposits in mesenteric arteries of eTNAP mice aged 13 weeks. *, lumen. E, Mesenteric arteries from eTNAP mice aged 13 weeks, with hematoxylin and eosin staining; (double arrow) neointima. *, lumen. F, Masson's trichrome staining of coronary, renal, mesenteric, and spenic arteries from eTNAP mice aged 13 weeks. *, lumen. AP indicates alkaline phosphatase; eTNAP, endothelial tissue‐nonspecific alkaline phosphatase; IB4, isolectin B4; WT, wild type.

Article Snippet: ECs were detected with FITC‐labeled isolectin B4 (IB4) from Bandeiraea simplicifolia (item FL1201; Vector Laboratories).

Techniques: Staining

Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

Article Title: Transgenic Overexpression of Tissue‐Nonspecific Alkaline Phosphatase ( TNAP ) in Vascular Endothelium Results in Generalized Arterial Calcification

doi: 10.1161/JAHA.115.002499

Figure Lengend Snippet: Expression of Runx2, collagen II , osteopontin, and sialic acid in coronary lesions of eTNAP mice. A through D, coronary lesions were identified by DIC imaging. A, Consecutive sections of the hearts from eTNAP mice aged 8 weeks were stained with Runx2 antibody or secondary antibody only, DNA binding dye DAPI was used as counterstain. B, Lesions from eTNAP mice aged 8 weeks were stained with collagen II antibody or secondary antibody alone and counterstained with DAPI. C, Osteopontin expression was detected in the lesions from eTNAP mice aged 13 weeks by direct immunofluorescent staining; IB 4 was used as counterstain. D, The presence of sialic acid was detected in the coronary lesions from eTNAP mice aged 13 weeks with fluorescein‐labeled SNA lectin; DAPI was used as nuclear counterstain. Affected (left panels) and unaffected (right panels) coronary arteries from the same tissue section are shown. DIC indicates differential interference contrast; eTNAP, endothelial tissue‐nonspecific alkaline phosphatase; IB4, isolectin B4; SNA, Sambucus nigra .

Article Snippet: ECs were detected with FITC‐labeled isolectin B4 (IB4) from Bandeiraea simplicifolia (item FL1201; Vector Laboratories).

Techniques: Expressing, Imaging, Staining, Binding Assay, Labeling

Journal:

Article Title: Aponecrotic, antiangiogenic and antiproliferative effects of a novel dextran derivative on breast cancer growth in vitro and in vivo

doi: 10.1038/sj.bjp.0704645

Figure Lengend Snippet: Inhibition of MCF-7ras tumour angiogenesis by NaPaC, CMDB and NaPa. Microvessel staining of tumours untreated (A) and treated with 150 mg kg−1 CMDB (B), 40 mg kg−1 NaPa (C) or 15 mg kg−1 NaPaC (D), twice a week, for 7 weeks, was performed using GSL-1 lectin. Necrotic areas in panel d are indicated with the asterisks. Magnification ×250 was used for panels A, B and C, and magnification ×100 was applied for panel D. The representative microvessels in panels A – C are marked with the arrows.

Article Snippet: Endothelial cells were specifically labelled with GSL-1 isolectin B4 (Vector Laboratories, Burlingame, CA, U.S.A.).

Techniques: Inhibition, Staining