Journal: Scientific Reports

Article Title: Impaired bile acid metabolism with defectives of mitochondrial-tRNA taurine modification and bile acid taurine conjugation in the taurine depleted cats

doi: 10.1038/s41598-020-61821-6

Figure Lengend Snippet: Free-cholesterol and oxysterol concentrations in the liver tissue. Bile acids are synthesized from cholesterol in the liver via two main pathways. In one pathway, cholesterol is initially converted to 7αHC by microsomal CYP7A1, which is the rate-limiting enzyme in BA synthesis. After several steps, the intermediate is then metabolized by mitochondrial CYP27A1 to finally yield CA. In another pathway, cholesterol is initially converted to 27HC by CYP27A1, and ultimately metabolized to CDCA. CDCA is also yielded by CYP27A1 from intermediates (e.g., 7αHC and C4) in the CA synthetic pathway. 7αHC, C4, and 27HC are the key intermediates through CYP7A1 and CYP27A1 in BA synthetic pathway. Lathosterol, 7-DHC, and Desmosterol are the intermediates in cholesterol synthesis. Data are the mean ± SE. * P < 0.05, ** P < 0.01, † P < 0.001 versus the Control group by unpaired Student t -test. Abbreviations : 3 β-HSD ; 3β-hydroxysteroid dehydrogenase, 7αHC ; 7α-hydroxycholesterol, 7DHC ; 7-dehydroxycholesterol, 27HC ; 27-hydroxycholesterol, C4 ; 7α-hydroxy-4-cholesten-3-one, CYP7A1 ; cholesterol 7 alpha-hydroxylase, CYP7B1 ; 25-hydroxycholesterol 7-alpha-hydroxylase, CYP8B1 ; 7α-hydroxy-4-cholesten-3-one 12α-hydroxylase, CYP27A1 ; cholesterol 27-hydroxylase, Free Chol ; free-cholesterol, Triol; 5β-cholestane-3α,7α,12α-triol, and see the legend of Fig. for other abbreviations.

Article Snippet: Cholic acid, glycocholic acid (GCA), taurocholic acid (TCA), CDCA, glycochenodeoxycholic acid (GCDCA), taurochenodeoxycholic acid (TCDCA), DCA, glycodeoxycholic acid (GDCA), taurodeoxycholic acid (TDCA), LCA, glycolithocholic acid (GLCA), taurolithocholic acid (TLCA), UDCA, glycoursodeoxycholic acid (GUDCA), 4β-hydroxycholesterol (4βHC), 7αHC, 24S-hydroxycholesterol (24SHC), 25-hydroxycholesterol (25HC), C4, lathosterol, 7DHC, desmosterol, and coprostanol were obtained from Steraloids, Inc. (Newport, RI, USA).

Techniques: Synthesized, Control

Journal: The Journal of Steroid Biochemistry and Molecular Biology

Article Title: First international descriptive and interventional survey for cholesterol and non-cholesterol sterol determination by gas- and liquid-chromatography–Urgent need for harmonisation of analytical methods

doi: 10.1016/j.jsbmb.2019.03.025

Figure Lengend Snippet: Results of absolute cholesterol and non-cholesterol sterols submitted by GC–MS and LC–MS methods.

Article Snippet: Six of the remaining eight laboratories determined the lathosterol to cholesterol ratio within a ± 15% range of the corrected mean value.

Techniques:

Journal: The Journal of Steroid Biochemistry and Molecular Biology

Article Title: First international descriptive and interventional survey for cholesterol and non-cholesterol sterol determination by gas- and liquid-chromatography–Urgent need for harmonisation of analytical methods

doi: 10.1016/j.jsbmb.2019.03.025

Figure Lengend Snippet: Results of cholesterol corrected non-cholesterol sterols and ratio lathosterol to campesterol submitted by GC–MS and LC–MS methods.

Article Snippet: Six of the remaining eight laboratories determined the lathosterol to cholesterol ratio within a ± 15% range of the corrected mean value.

Techniques:

Journal: The Journal of Steroid Biochemistry and Molecular Biology

Article Title: First international descriptive and interventional survey for cholesterol and non-cholesterol sterol determination by gas- and liquid-chromatography–Urgent need for harmonisation of analytical methods

doi: 10.1016/j.jsbmb.2019.03.025

Figure Lengend Snippet: Distribution of absolute cholesterol and non-cholesterol sterol concentrations analyzed by GC–MS and LC–MS methods in the first descriptive and second interventional survey. Values a–e in the figures A1-E2 are regarded as outliers.

Article Snippet: Six of the remaining eight laboratories determined the lathosterol to cholesterol ratio within a ± 15% range of the corrected mean value.

Techniques: Gas Chromatography-Mass Spectrometry, Liquid Chromatography with Mass Spectroscopy

Journal: The Journal of Steroid Biochemistry and Molecular Biology

Article Title: First international descriptive and interventional survey for cholesterol and non-cholesterol sterol determination by gas- and liquid-chromatography–Urgent need for harmonisation of analytical methods

doi: 10.1016/j.jsbmb.2019.03.025

Figure Lengend Snippet: Distribution of calculated cholesterol corrected non-cholesterol concentrations and ratio lathosterol to campesterol in the first descriptive and second interventional survey. Values a–e in the figures A1-D2 are regarded as outliers.

Article Snippet: Six of the remaining eight laboratories determined the lathosterol to cholesterol ratio within a ± 15% range of the corrected mean value.

Techniques: