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Rabbit anti-Human LAMTOR3 Polyclonal Antibody
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Image Search Results
Journal: Frontiers in Immunology
Article Title: Arginase 1 Insufficiency Precipitates Amyloid- β Deposition and Hastens Behavioral Impairment in a Mouse Model of Amyloidosis
doi: 10.3389/fimmu.2020.582998
Figure Lengend Snippet: Hemizygous deletion of Arg1 in myeloid cells reduced total Arg1 and Ragulator-Rag complex components during brain amyloidosis. We measured Arg1 mRNA by qRT-PCR, ARG1 protein by western blotting, and its catabolizing substrate arginine by LC-MS/MS. We also measured key Ragulator-Rag complex component proteins critical for microglial phagocytosis by western blotting. Mouse brain samples used were comprised of four experimental groups: nTg/Arg1 +/+ /LysMcre Tg/+ , nTg/Arg1 fl/+ /LysMcre Tg/+ , APP +/ − /Arg1 +/+ /LysMcre Tg/+ , APP +/ − /Arg1 fl/+ /LysMcre Tg/+ . (A) Quantification analysis of qRT-PCR data for Arg1 mRNA that was normalized to β -actin. (B) Quantification analysis of western blot data for ARG1 protein. (C) Quantification analysis of LC-MS/MS data for amino acid arginine. (D) Representative images of western blotting for ARG1, LAMTOR2, LAMTOR3, LAMTOR4, and RAG (A) (E–H) Quantification analysis of LAMTOR2 (E) , LAMTOR3 (F) , LAMTOR4 (G) and RAG A (H) from images in (D) . For mice in each group, n = 8 – 10 for nTg/Arg1 +/+ /LysMcre Tg/+ , n=8-10 for nTg/Arg1 fl/+ /LysMcre Tg/+ , n=6-7 for APP +/ − /Arg1 +/+ /LysMcre Tg/+ , n=7-9 for APP +/ − /Arg1 fl/+ /LysMcre Tg/+ . Asterisk sign (*) denotes statistical significance observed for the main effect of APP genotype and associated pairwise comparisons. The number sign (#) denotes statistical significance observed for the main effect of Arg1 insufficiency genotype and associated pairwise comparisons. Ampersand sign (&) denotes statistically significant interaction between APP genotype and Arg1 insufficiency genotype. * /# p < 0.05; ** /&& p < 0.01; ### p < 0.001. Two-way ANOVA followed by pairwise comparisons. Values represent mean ± SEM.
Article Snippet: The following primary antibodies were used: Anti-Liver Arginase (abcam, #ab124917), LAMTOR2 (Cell Signaling Technologies, #8145),
Techniques: Quantitative RT-PCR, Western Blot, Liquid Chromatography with Mass Spectroscopy
Journal: The EMBO Journal
Article Title: Micropeptide hSPAR regulates glutamine levels and suppresses mammary tumor growth via a TRIM21-P27KIP1-mTOR axis
doi: 10.1038/s44318-024-00359-z
Figure Lengend Snippet: Reagents and tools table
Article Snippet:
Techniques: Recombinant, Sequencing, Modification, Membrane, Lysis, Transfection, Magnetic Beads, Software, Cytometry, In Vitro, cDNA Synthesis, Plasmid Preparation, Isolation, Mutagenesis, Silver Staining