ladder Search Results


kb plus dna ladder  (Gold Biotechnology Inc)
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Gold Biotechnology Inc kb plus dna ladder
Kb Plus Dna Ladder, supplied by Gold Biotechnology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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kb plus dna ladder  (Thermo Fisher)
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Thermo Fisher kb plus dna ladder
Kb Plus Dna Ladder, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dna ladder  (New England Biolabs)
96
New England Biolabs dna ladder
Dna Ladder, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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kb plus dna ladder  (New England Biolabs)
96
New England Biolabs kb plus dna ladder
Kb Plus Dna Ladder, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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base pair dna ladder  (Thermo Fisher)
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Thermo Fisher base pair dna ladder
FIG. 4. TNF-a decreases MSR mRNA synthesis in PMA-differ- entiated THP-1 macrophages at steady state level. A, total RNA was isolated from THP-1 cells grown in serum-free medium (Control, THP-1 monocytes, samples 1 and 5), medium containing PMA (150 nM) (PMA, THP-1 macrophages, samples 2 and 6), medium containing PMA (150 nM) 1 TNF-a (200 units/ml) (PMA 1 TNF-a, samples 3 and 7), or medium containing TNF-a (200 units/ml) (TNF-a, samples 4 and 8) for 20 and 48 h, respectively. Northern blots were hybridized with 32P- labeled cDNAs of MSR or 28 S. The respective autoradiograms with MSR and 28 S bands are labeled and indicated with arrows. B, histo- grams represent quantification by PhosphorImager® of MSR mRNA normalized by comparison with 28 S ribosomal RNA. All data are expressed as a percentage of sample 2, i.e. PMA-differentiated THP-1 macrophages. Similar results were obtained in three separate experi- ments. C, RT-PCR analysis of the expression of macrophage scavenger receptor types SR-AI and SR-AII mRNA in THP-1 monocytes, PMA- differentiated THP-1 macrophages in the absence and the presence of TNF-a. Ethidium bromide-stained agarose gel with MSR type SR-AI at 447 base pairs and MSR type SR-AII at about 285 base pairs. Lane 1, 100 base pair DNA ladder; lane 2, THP-1 monocytes; lane 3, PMA- differentiated THP-1 macrophages (24 h); lane 4, THP-1 cells with PMA 1 TNF-a (24 h); lane 5, DNA molecular weight marker V from Boeh- ringer Mannheim. The detailed method is described under ‘‘Experimen- tal Procedures.’’ There was no detectable MSR type SR-AI and SR-AII mRNA in THP-1 cells with TNF-a (data not shown). The arrows indi- cate MSR types SR-AI and SR-AII. The results shown are representa- tive of three independent experiments.
Base Pair Dna Ladder, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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protein ladder  (LI-COR)
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LI-COR protein ladder
FIG. 4. TNF-a decreases MSR mRNA synthesis in PMA-differ- entiated THP-1 macrophages at steady state level. A, total RNA was isolated from THP-1 cells grown in serum-free medium (Control, THP-1 monocytes, samples 1 and 5), medium containing PMA (150 nM) (PMA, THP-1 macrophages, samples 2 and 6), medium containing PMA (150 nM) 1 TNF-a (200 units/ml) (PMA 1 TNF-a, samples 3 and 7), or medium containing TNF-a (200 units/ml) (TNF-a, samples 4 and 8) for 20 and 48 h, respectively. Northern blots were hybridized with 32P- labeled cDNAs of MSR or 28 S. The respective autoradiograms with MSR and 28 S bands are labeled and indicated with arrows. B, histo- grams represent quantification by PhosphorImager® of MSR mRNA normalized by comparison with 28 S ribosomal RNA. All data are expressed as a percentage of sample 2, i.e. PMA-differentiated THP-1 macrophages. Similar results were obtained in three separate experi- ments. C, RT-PCR analysis of the expression of macrophage scavenger receptor types SR-AI and SR-AII mRNA in THP-1 monocytes, PMA- differentiated THP-1 macrophages in the absence and the presence of TNF-a. Ethidium bromide-stained agarose gel with MSR type SR-AI at 447 base pairs and MSR type SR-AII at about 285 base pairs. Lane 1, 100 base pair DNA ladder; lane 2, THP-1 monocytes; lane 3, PMA- differentiated THP-1 macrophages (24 h); lane 4, THP-1 cells with PMA 1 TNF-a (24 h); lane 5, DNA molecular weight marker V from Boeh- ringer Mannheim. The detailed method is described under ‘‘Experimen- tal Procedures.’’ There was no detectable MSR type SR-AI and SR-AII mRNA in THP-1 cells with TNF-a (data not shown). The arrows indi- cate MSR types SR-AI and SR-AII. The results shown are representa- tive of three independent experiments.
Protein Ladder, supplied by LI-COR, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dna ladder  (Thermo Fisher)
99
Thermo Fisher dna ladder
FIG. 4. TNF-a decreases MSR mRNA synthesis in PMA-differ- entiated THP-1 macrophages at steady state level. A, total RNA was isolated from THP-1 cells grown in serum-free medium (Control, THP-1 monocytes, samples 1 and 5), medium containing PMA (150 nM) (PMA, THP-1 macrophages, samples 2 and 6), medium containing PMA (150 nM) 1 TNF-a (200 units/ml) (PMA 1 TNF-a, samples 3 and 7), or medium containing TNF-a (200 units/ml) (TNF-a, samples 4 and 8) for 20 and 48 h, respectively. Northern blots were hybridized with 32P- labeled cDNAs of MSR or 28 S. The respective autoradiograms with MSR and 28 S bands are labeled and indicated with arrows. B, histo- grams represent quantification by PhosphorImager® of MSR mRNA normalized by comparison with 28 S ribosomal RNA. All data are expressed as a percentage of sample 2, i.e. PMA-differentiated THP-1 macrophages. Similar results were obtained in three separate experi- ments. C, RT-PCR analysis of the expression of macrophage scavenger receptor types SR-AI and SR-AII mRNA in THP-1 monocytes, PMA- differentiated THP-1 macrophages in the absence and the presence of TNF-a. Ethidium bromide-stained agarose gel with MSR type SR-AI at 447 base pairs and MSR type SR-AII at about 285 base pairs. Lane 1, 100 base pair DNA ladder; lane 2, THP-1 monocytes; lane 3, PMA- differentiated THP-1 macrophages (24 h); lane 4, THP-1 cells with PMA 1 TNF-a (24 h); lane 5, DNA molecular weight marker V from Boeh- ringer Mannheim. The detailed method is described under ‘‘Experimen- tal Procedures.’’ There was no detectable MSR type SR-AI and SR-AII mRNA in THP-1 cells with TNF-a (data not shown). The arrows indi- cate MSR types SR-AI and SR-AII. The results shown are representa- tive of three independent experiments.
Dna Ladder, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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low molecular weight dna ladder  (New England Biolabs)
96
New England Biolabs low molecular weight dna ladder
FIG. 4. TNF-a decreases MSR mRNA synthesis in PMA-differ- entiated THP-1 macrophages at steady state level. A, total RNA was isolated from THP-1 cells grown in serum-free medium (Control, THP-1 monocytes, samples 1 and 5), medium containing PMA (150 nM) (PMA, THP-1 macrophages, samples 2 and 6), medium containing PMA (150 nM) 1 TNF-a (200 units/ml) (PMA 1 TNF-a, samples 3 and 7), or medium containing TNF-a (200 units/ml) (TNF-a, samples 4 and 8) for 20 and 48 h, respectively. Northern blots were hybridized with 32P- labeled cDNAs of MSR or 28 S. The respective autoradiograms with MSR and 28 S bands are labeled and indicated with arrows. B, histo- grams represent quantification by PhosphorImager® of MSR mRNA normalized by comparison with 28 S ribosomal RNA. All data are expressed as a percentage of sample 2, i.e. PMA-differentiated THP-1 macrophages. Similar results were obtained in three separate experi- ments. C, RT-PCR analysis of the expression of macrophage scavenger receptor types SR-AI and SR-AII mRNA in THP-1 monocytes, PMA- differentiated THP-1 macrophages in the absence and the presence of TNF-a. Ethidium bromide-stained agarose gel with MSR type SR-AI at 447 base pairs and MSR type SR-AII at about 285 base pairs. Lane 1, 100 base pair DNA ladder; lane 2, THP-1 monocytes; lane 3, PMA- differentiated THP-1 macrophages (24 h); lane 4, THP-1 cells with PMA 1 TNF-a (24 h); lane 5, DNA molecular weight marker V from Boeh- ringer Mannheim. The detailed method is described under ‘‘Experimen- tal Procedures.’’ There was no detectable MSR type SR-AI and SR-AII mRNA in THP-1 cells with TNF-a (data not shown). The arrows indi- cate MSR types SR-AI and SR-AII. The results shown are representa- tive of three independent experiments.
Low Molecular Weight Dna Ladder, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lane 1  (New England Biolabs)
96
New England Biolabs lane 1
FIG. 4. TNF-a decreases MSR mRNA synthesis in PMA-differ- entiated THP-1 macrophages at steady state level. A, total RNA was isolated from THP-1 cells grown in serum-free medium (Control, THP-1 monocytes, samples 1 and 5), medium containing PMA (150 nM) (PMA, THP-1 macrophages, samples 2 and 6), medium containing PMA (150 nM) 1 TNF-a (200 units/ml) (PMA 1 TNF-a, samples 3 and 7), or medium containing TNF-a (200 units/ml) (TNF-a, samples 4 and 8) for 20 and 48 h, respectively. Northern blots were hybridized with 32P- labeled cDNAs of MSR or 28 S. The respective autoradiograms with MSR and 28 S bands are labeled and indicated with arrows. B, histo- grams represent quantification by PhosphorImager® of MSR mRNA normalized by comparison with 28 S ribosomal RNA. All data are expressed as a percentage of sample 2, i.e. PMA-differentiated THP-1 macrophages. Similar results were obtained in three separate experi- ments. C, RT-PCR analysis of the expression of macrophage scavenger receptor types SR-AI and SR-AII mRNA in THP-1 monocytes, PMA- differentiated THP-1 macrophages in the absence and the presence of TNF-a. Ethidium bromide-stained agarose gel with MSR type SR-AI at 447 base pairs and MSR type SR-AII at about 285 base pairs. Lane 1, 100 base pair DNA ladder; lane 2, THP-1 monocytes; lane 3, PMA- differentiated THP-1 macrophages (24 h); lane 4, THP-1 cells with PMA 1 TNF-a (24 h); lane 5, DNA molecular weight marker V from Boeh- ringer Mannheim. The detailed method is described under ‘‘Experimen- tal Procedures.’’ There was no detectable MSR type SR-AI and SR-AII mRNA in THP-1 cells with TNF-a (data not shown). The arrows indi- cate MSR types SR-AI and SR-AII. The results shown are representa- tive of three independent experiments.
Lane 1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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protein ladder chameleon duo ladder  (LI-COR)
99
LI-COR protein ladder chameleon duo ladder
FIG. 4. TNF-a decreases MSR mRNA synthesis in PMA-differ- entiated THP-1 macrophages at steady state level. A, total RNA was isolated from THP-1 cells grown in serum-free medium (Control, THP-1 monocytes, samples 1 and 5), medium containing PMA (150 nM) (PMA, THP-1 macrophages, samples 2 and 6), medium containing PMA (150 nM) 1 TNF-a (200 units/ml) (PMA 1 TNF-a, samples 3 and 7), or medium containing TNF-a (200 units/ml) (TNF-a, samples 4 and 8) for 20 and 48 h, respectively. Northern blots were hybridized with 32P- labeled cDNAs of MSR or 28 S. The respective autoradiograms with MSR and 28 S bands are labeled and indicated with arrows. B, histo- grams represent quantification by PhosphorImager® of MSR mRNA normalized by comparison with 28 S ribosomal RNA. All data are expressed as a percentage of sample 2, i.e. PMA-differentiated THP-1 macrophages. Similar results were obtained in three separate experi- ments. C, RT-PCR analysis of the expression of macrophage scavenger receptor types SR-AI and SR-AII mRNA in THP-1 monocytes, PMA- differentiated THP-1 macrophages in the absence and the presence of TNF-a. Ethidium bromide-stained agarose gel with MSR type SR-AI at 447 base pairs and MSR type SR-AII at about 285 base pairs. Lane 1, 100 base pair DNA ladder; lane 2, THP-1 monocytes; lane 3, PMA- differentiated THP-1 macrophages (24 h); lane 4, THP-1 cells with PMA 1 TNF-a (24 h); lane 5, DNA molecular weight marker V from Boeh- ringer Mannheim. The detailed method is described under ‘‘Experimen- tal Procedures.’’ There was no detectable MSR type SR-AI and SR-AII mRNA in THP-1 cells with TNF-a (data not shown). The arrows indi- cate MSR types SR-AI and SR-AII. The results shown are representa- tive of three independent experiments.
Protein Ladder Chameleon Duo Ladder, supplied by LI-COR, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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biotinylated protein ladder detection pack  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc biotinylated protein ladder detection pack
FIG. 4. TNF-a decreases MSR mRNA synthesis in PMA-differ- entiated THP-1 macrophages at steady state level. A, total RNA was isolated from THP-1 cells grown in serum-free medium (Control, THP-1 monocytes, samples 1 and 5), medium containing PMA (150 nM) (PMA, THP-1 macrophages, samples 2 and 6), medium containing PMA (150 nM) 1 TNF-a (200 units/ml) (PMA 1 TNF-a, samples 3 and 7), or medium containing TNF-a (200 units/ml) (TNF-a, samples 4 and 8) for 20 and 48 h, respectively. Northern blots were hybridized with 32P- labeled cDNAs of MSR or 28 S. The respective autoradiograms with MSR and 28 S bands are labeled and indicated with arrows. B, histo- grams represent quantification by PhosphorImager® of MSR mRNA normalized by comparison with 28 S ribosomal RNA. All data are expressed as a percentage of sample 2, i.e. PMA-differentiated THP-1 macrophages. Similar results were obtained in three separate experi- ments. C, RT-PCR analysis of the expression of macrophage scavenger receptor types SR-AI and SR-AII mRNA in THP-1 monocytes, PMA- differentiated THP-1 macrophages in the absence and the presence of TNF-a. Ethidium bromide-stained agarose gel with MSR type SR-AI at 447 base pairs and MSR type SR-AII at about 285 base pairs. Lane 1, 100 base pair DNA ladder; lane 2, THP-1 monocytes; lane 3, PMA- differentiated THP-1 macrophages (24 h); lane 4, THP-1 cells with PMA 1 TNF-a (24 h); lane 5, DNA molecular weight marker V from Boeh- ringer Mannheim. The detailed method is described under ‘‘Experimen- tal Procedures.’’ There was no detectable MSR type SR-AI and SR-AII mRNA in THP-1 cells with TNF-a (data not shown). The arrows indi- cate MSR types SR-AI and SR-AII. The results shown are representa- tive of three independent experiments.
Biotinylated Protein Ladder Detection Pack, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


FIG. 4. TNF-a decreases MSR mRNA synthesis in PMA-differ- entiated THP-1 macrophages at steady state level. A, total RNA was isolated from THP-1 cells grown in serum-free medium (Control, THP-1 monocytes, samples 1 and 5), medium containing PMA (150 nM) (PMA, THP-1 macrophages, samples 2 and 6), medium containing PMA (150 nM) 1 TNF-a (200 units/ml) (PMA 1 TNF-a, samples 3 and 7), or medium containing TNF-a (200 units/ml) (TNF-a, samples 4 and 8) for 20 and 48 h, respectively. Northern blots were hybridized with 32P- labeled cDNAs of MSR or 28 S. The respective autoradiograms with MSR and 28 S bands are labeled and indicated with arrows. B, histo- grams represent quantification by PhosphorImager® of MSR mRNA normalized by comparison with 28 S ribosomal RNA. All data are expressed as a percentage of sample 2, i.e. PMA-differentiated THP-1 macrophages. Similar results were obtained in three separate experi- ments. C, RT-PCR analysis of the expression of macrophage scavenger receptor types SR-AI and SR-AII mRNA in THP-1 monocytes, PMA- differentiated THP-1 macrophages in the absence and the presence of TNF-a. Ethidium bromide-stained agarose gel with MSR type SR-AI at 447 base pairs and MSR type SR-AII at about 285 base pairs. Lane 1, 100 base pair DNA ladder; lane 2, THP-1 monocytes; lane 3, PMA- differentiated THP-1 macrophages (24 h); lane 4, THP-1 cells with PMA 1 TNF-a (24 h); lane 5, DNA molecular weight marker V from Boeh- ringer Mannheim. The detailed method is described under ‘‘Experimen- tal Procedures.’’ There was no detectable MSR type SR-AI and SR-AII mRNA in THP-1 cells with TNF-a (data not shown). The arrows indi- cate MSR types SR-AI and SR-AII. The results shown are representa- tive of three independent experiments.

Journal: The Journal of biological chemistry

Article Title: Inhibition of macrophage scavenger receptor activity by tumor necrosis factor-alpha is transcriptionally and post-transcriptionally regulated.

doi: 10.1074/jbc.271.13.7767

Figure Lengend Snippet: FIG. 4. TNF-a decreases MSR mRNA synthesis in PMA-differ- entiated THP-1 macrophages at steady state level. A, total RNA was isolated from THP-1 cells grown in serum-free medium (Control, THP-1 monocytes, samples 1 and 5), medium containing PMA (150 nM) (PMA, THP-1 macrophages, samples 2 and 6), medium containing PMA (150 nM) 1 TNF-a (200 units/ml) (PMA 1 TNF-a, samples 3 and 7), or medium containing TNF-a (200 units/ml) (TNF-a, samples 4 and 8) for 20 and 48 h, respectively. Northern blots were hybridized with 32P- labeled cDNAs of MSR or 28 S. The respective autoradiograms with MSR and 28 S bands are labeled and indicated with arrows. B, histo- grams represent quantification by PhosphorImager® of MSR mRNA normalized by comparison with 28 S ribosomal RNA. All data are expressed as a percentage of sample 2, i.e. PMA-differentiated THP-1 macrophages. Similar results were obtained in three separate experi- ments. C, RT-PCR analysis of the expression of macrophage scavenger receptor types SR-AI and SR-AII mRNA in THP-1 monocytes, PMA- differentiated THP-1 macrophages in the absence and the presence of TNF-a. Ethidium bromide-stained agarose gel with MSR type SR-AI at 447 base pairs and MSR type SR-AII at about 285 base pairs. Lane 1, 100 base pair DNA ladder; lane 2, THP-1 monocytes; lane 3, PMA- differentiated THP-1 macrophages (24 h); lane 4, THP-1 cells with PMA 1 TNF-a (24 h); lane 5, DNA molecular weight marker V from Boeh- ringer Mannheim. The detailed method is described under ‘‘Experimen- tal Procedures.’’ There was no detectable MSR type SR-AI and SR-AII mRNA in THP-1 cells with TNF-a (data not shown). The arrows indi- cate MSR types SR-AI and SR-AII. The results shown are representa- tive of three independent experiments.

Article Snippet: Medium RPMI 1640 medium, L-glutamine, penicillin, streptomycin, and fetal calf serum were purchased from Life Technologies, Inc. A 100-base pair DNA ladder was purchased from Life Technologies, Inc., MD.

Techniques: Isolation, Control, Northern Blot, Labeling, Comparison, Reverse Transcription Polymerase Chain Reaction, Expressing, Staining, Agarose Gel Electrophoresis, Molecular Weight, Marker