Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: A low-salt diet with candesartan administration is associated with acute kidney injury in nephritis by increasing nitric oxide.

doi: 10.1016/j.biopha.2023.114484

Figure Lengend Snippet: Fig. 6. Blood pressure, kidney function and histology in low-salt with candesartan with or without L-NAME. A. NO production in kidney tissue in 4 groups nephritis rats: normal-salt with candesartan (Dox+Can+NS), low-salt with candesartan (Dox+Can+LS), low-salt with candesartan and L-NAME (Dox+Can+LS+L- NAME), and normal-salt with candesartan and L-NAME (Dox+Can+NS +L-NAME). B-D. Blood pressure included SBP, DBP and MAP in the 4 groups. E-G. Kidney function index included SCr and BUN and calculated Ccr. H. Kidney histology changes in the 4 groups. The black arrowhead indicates glomerular shrinkage, and the yellow arrowhead indicates renal tubular necrosis. Scale bar= 100 µm. n = 4–6/group. * P < 0.05, * * P < 0.01.

Article Snippet: Both groups of rats were given 1 mg/kg/d candesartan i.p. NG-nitro-L-arginine methyl ester (L-NAME) (HY-18729A, MCE, USA, 10 mg/kg/d.ip), an inhibitor of endothelial nitric oxide synthase (eNOS), was intraperitoneally injected for 7 days, and the rats were divided into 4 groups: NS, LS, NS+L-NAME, and LS+L-NAME.

Techniques:

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: A low-salt diet with candesartan administration is associated with acute kidney injury in nephritis by increasing nitric oxide.

doi: 10.1016/j.biopha.2023.114484

Figure Lengend Snippet: Fig. 7. Apoptosis detection in vivo and in vitro. The nephritis rats were divided into 4 groups, Dox+Can+NS (n = 4), Dox+Can+LS (n = 4), Dox+Can+LS+L- NAME (n = 6), and Dox+Can+NS +L-NAME (n = 6), and sacrificed on day 7. A-B. TUNEL staining and positive cell count in vivo. The brown nuclei indicated by the black arrows show TUNEL-positive cells, and the number per high-power field was greater in the low-salt treatment with candesartan but reversed by L-NAME and the eNOS inhibitor. Scale bar = 50 µm. C-E. western blotting and quantitative analysis of BAX, Bcl-2, and cleaved caspase-3 in vivo. F-H. HK-2 cells were pretreated with the NO donor SNP for different times, and BAX, Bcl-2, cleaved caspase-3 and Caspase-3 were analyzed by western blotting. n = 3–4/group. * P < 0.05, * * P < 0.01.

Article Snippet: Both groups of rats were given 1 mg/kg/d candesartan i.p. NG-nitro-L-arginine methyl ester (L-NAME) (HY-18729A, MCE, USA, 10 mg/kg/d.ip), an inhibitor of endothelial nitric oxide synthase (eNOS), was intraperitoneally injected for 7 days, and the rats were divided into 4 groups: NS, LS, NS+L-NAME, and LS+L-NAME.

Techniques: In Vivo, In Vitro, TUNEL Assay, Staining, Cell Characterization, Western Blot

Journal: The Journal of Neuroscience

Article Title: A Critical Role of the Nitric Oxide/cGMP Pathway in Corticostriatal Long-Term Depression

doi: 10.1523/jneurosci.19-07-02489.1999

Figure Lengend Snippet: Figure 6. Corticostriatal LTD is inhibited by either L-NAME or 7-NINA. A, Bath application of 50 mM L-NAME blocks both tetanus-induced (top graph) and zaprinast-induced LTD (bottom graph). B, These forms of synaptic plasticity are also blocked by 10 mM 7-NINA.

Article Snippet: 6-Cyano-7-nitroquinoxaline-2,3-dione (CNQX), 1H[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ), N(G)-nitro-L-arginine methyl ester hydrochloride (L-NAME), 7-nitroindazole monosodium salt (7-NINA), and S-nitroso-N-acetylpenicillamine (SNAP) were from Tocris Cookson. cGMP and guanosine 39,59-cyclic monophosphorothioate, 8-bromo, Rp-isomer, sodium salt (Rp-8-Br-cGMPS) were from Calbiochem (La Jolla, CA).

Techniques:

Journal: The Journal of Neuroscience

Article Title: A Critical Role of the Nitric Oxide/cGMP Pathway in Corticostriatal Long-Term Depression

doi: 10.1523/jneurosci.19-07-02489.1999

Figure Lengend Snippet: Figure 7. NO donors induce striatal LTD. A, Bath application of 100 mM SNAP induces a fast and long-term decrease of EPSP amplitude (F). This form of LTD is antagonized by 100 mM intracellular ODQ (E) but not by bath applica- tion of 50 mM L-NAME (M). B, Bath application of 100 mM hydroxylamine also induces a persis- tent decrease of EPSP amplitude. Note, however, that this inhibitory action has a slower time course than that observed with SNAP. C, LTD induced by bath application of SNAP occludes HFS-induced (top graph) or zaprinast-induced LTD (bottom graph).

Article Snippet: 6-Cyano-7-nitroquinoxaline-2,3-dione (CNQX), 1H[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ), N(G)-nitro-L-arginine methyl ester hydrochloride (L-NAME), 7-nitroindazole monosodium salt (7-NINA), and S-nitroso-N-acetylpenicillamine (SNAP) were from Tocris Cookson. cGMP and guanosine 39,59-cyclic monophosphorothioate, 8-bromo, Rp-isomer, sodium salt (Rp-8-Br-cGMPS) were from Calbiochem (La Jolla, CA).

Techniques:

Journal: Pharmacognosy Research

Article Title: Effects of L-citrulline diet on stress-induced cold hypersensitivity in mice

doi: 10.4103/0974-8490.138269

Figure Lengend Snippet: The effects of single administration of L-NG-nitroarginine methyl ester (L-NAME) on the time course of changes in core body temperature after cold exposure without immobilization stress preconditioning. L-NAME was administered intraperitoneally 1 h prior to forced cold-water swimming. Open triangles: L-NAME, closed triangles: Vehicle. Values shown are the means ± standard deviation ( n = 6). Statistical analysis: Unpaired t -test (* P < 0.05; ** P < 0.01 vs. the control)

Article Snippet: L-NG-nitroarginine methyl ester (L-NAME hydrochloride, purity >98.0%; Wako Pure Chemical Industries Ltd, Tokyo, Japan) was dissolved in saline and administered intraperitoneally 1 h prior to the forced cold-water swimming.

Techniques: Standard Deviation

Journal: Pharmacognosy Research

Article Title: Effects of L-citrulline diet on stress-induced cold hypersensitivity in mice

doi: 10.4103/0974-8490.138269

Figure Lengend Snippet: The effects of administration of a single L-NG-nitroarginine methyl ester (L-NAME) dose on the time course of changes in tail skin surface temperature after cold exposure without immobilization stress preconditioning. L-NAME was administrated intraperitoneally 1 h prior to forced cold-water swimming. Open triangles: L-NAME, closed triangles: Vehicle. Values shown are the means ± standard deviation ( n = 6). Statistical analysis: Unpaired t -test (* P < 0.05; ** P < 0.01 vs. the control)

Article Snippet: L-NG-nitroarginine methyl ester (L-NAME hydrochloride, purity >98.0%; Wako Pure Chemical Industries Ltd, Tokyo, Japan) was dissolved in saline and administered intraperitoneally 1 h prior to the forced cold-water swimming.

Techniques: Standard Deviation