Journal: Nutrients

Article Title: Lipoic Acid Exacerbates Oxidative Stress and Lipid Accumulation in the Liver of Wistar Rats Fed a Hypercaloric Choline-Deficient Diet

doi: 10.3390/nu13061999

Figure Lengend Snippet: Effects of α-lipoic acid (LA) supplementation on body weight and nutritional profile.

Article Snippet: Animals were divided into three groups ( n = 8 in each): control pair-feeding group of rats fed a control diet (C) ( ); rats fed a hypercaloric choline-deficient diet (HCCD); rats fed a hypercaloric choline-deficient diet supplemented with α-lipoic acid (LA) (Chem Impex International, Inc., Wood Dale, USA, Cat# 29862) (HCCD+LA; consumed average daily dose of LA-61 mg/kg body weight).

Techniques:

Journal: Nutrients

Article Title: Lipoic Acid Exacerbates Oxidative Stress and Lipid Accumulation in the Liver of Wistar Rats Fed a Hypercaloric Choline-Deficient Diet

doi: 10.3390/nu13061999

Figure Lengend Snippet: Effects of α-lipoic acid (LA) supplementation on: ( a ) liver/body weight ratio; ( b ) visceral adipose tissue (VAT)/body weight ratio. Animals were divided into three groups: control group of rats fed a control diet (C); rats fed a hypercaloric choline-deficient diet (HCCD); rats fed a hypercaloric choline-deficient diet supplemented with α-lipoic acid (HCCD+LA; consumed average daily dose of LA-61 mg/kg body weight). VAT: visceral (epididymal and retroperitoneal) adipose tissue. Values are mean ± standard error of the mean. p < 0.05 vs. * C.

Article Snippet: Animals were divided into three groups ( n = 8 in each): control pair-feeding group of rats fed a control diet (C) ( ); rats fed a hypercaloric choline-deficient diet (HCCD); rats fed a hypercaloric choline-deficient diet supplemented with α-lipoic acid (LA) (Chem Impex International, Inc., Wood Dale, USA, Cat# 29862) (HCCD+LA; consumed average daily dose of LA-61 mg/kg body weight).

Techniques:

Journal: Nutrients

Article Title: Lipoic Acid Exacerbates Oxidative Stress and Lipid Accumulation in the Liver of Wistar Rats Fed a Hypercaloric Choline-Deficient Diet

doi: 10.3390/nu13061999

Figure Lengend Snippet: Effects of α-lipoic acid (LA) supplementation on liver lipid content.

Article Snippet: Animals were divided into three groups ( n = 8 in each): control pair-feeding group of rats fed a control diet (C) ( ); rats fed a hypercaloric choline-deficient diet (HCCD); rats fed a hypercaloric choline-deficient diet supplemented with α-lipoic acid (LA) (Chem Impex International, Inc., Wood Dale, USA, Cat# 29862) (HCCD+LA; consumed average daily dose of LA-61 mg/kg body weight).

Techniques:

Journal: Nutrients

Article Title: Lipoic Acid Exacerbates Oxidative Stress and Lipid Accumulation in the Liver of Wistar Rats Fed a Hypercaloric Choline-Deficient Diet

doi: 10.3390/nu13061999

Figure Lengend Snippet: Effects of α-lipoic acid (LA) supplementation on plasma levels of ( a ) glucose, ( b ) triglycerides (TG), ( c ) free fatty acids (FFA), ( d ) total cholesterol (T-Chol), ( e ) high-density lipoprotein cholesterol (HDL-Chol), ( f ) low-density lipoprotein cholesterol (LDL-Chol). Animals were divided into three groups: control group of rats fed a control diet (C); rats fed a hypercaloric choline-deficient diet (HCCD); rats fed a hypercaloric choline-deficient diet supplemented with α-lipoic acid (HCCD+LA; consumed average daily dose of LA-61 mg/kg body weight). Values are mean ± standard error of the mean. p < 0.05 vs. * C; # HCCD+LA vs. HCCD.

Article Snippet: Animals were divided into three groups ( n = 8 in each): control pair-feeding group of rats fed a control diet (C) ( ); rats fed a hypercaloric choline-deficient diet (HCCD); rats fed a hypercaloric choline-deficient diet supplemented with α-lipoic acid (LA) (Chem Impex International, Inc., Wood Dale, USA, Cat# 29862) (HCCD+LA; consumed average daily dose of LA-61 mg/kg body weight).

Techniques:

Journal: Nutrients

Article Title: Lipoic Acid Exacerbates Oxidative Stress and Lipid Accumulation in the Liver of Wistar Rats Fed a Hypercaloric Choline-Deficient Diet

doi: 10.3390/nu13061999

Figure Lengend Snippet: Effects of α-lipoic acid (LA) supplementation on ( a ) alanine aminotransferase (ALT) activity in plasma; ( b ) aspartate aminotransferase (AST) activity in plasma; ( c ) reduced glutathione (GSH) level in the liver; ( d ) reduced/oxidized glutathione ratio (GSH/GSSG) in the liver; ( e ) total antioxidant (AOA) activity in plasma; ( f ) total antioxidant (AOA) activity in the liver; ( g ) cytochrome P450 2E1 (CYP2E1) activity in the liver. Animals were divided into three groups: control group of rats fed a control diet (C); rats fed a hypercaloric choline-deficient diet (HCCD); rats fed a hypercaloric choline-deficient diet supplemented with α-lipoic acid (HCCD+LA; consumed average daily dose of LA-61 mg/kg body weight). Values are mean ± standard error of the mean. p < 0.05 vs. * C; # HCCD+LA vs. HCCD.

Article Snippet: Animals were divided into three groups ( n = 8 in each): control pair-feeding group of rats fed a control diet (C) ( ); rats fed a hypercaloric choline-deficient diet (HCCD); rats fed a hypercaloric choline-deficient diet supplemented with α-lipoic acid (LA) (Chem Impex International, Inc., Wood Dale, USA, Cat# 29862) (HCCD+LA; consumed average daily dose of LA-61 mg/kg body weight).

Techniques: Activity Assay

Journal: Nutrients

Article Title: Lipoic Acid Exacerbates Oxidative Stress and Lipid Accumulation in the Liver of Wistar Rats Fed a Hypercaloric Choline-Deficient Diet

doi: 10.3390/nu13061999

Figure Lengend Snippet: Effects of α-lipoic acid (LA) supplementation on ( a ) catalase (CAT) activity in the liver; ( b ) glutathione peroxidase (GPx) activity in the liver; ( c ) superoxide dismutase (SOD) activity in the liver; ( d ) paraoxonase-1 (PON-1) activity in plasma; ( e ) paraoxonase-1 (PON-1) activity in the liver. Animals were divided into three groups: control group of rats fed a control diet (C); rats fed a hypercaloric choline-deficient diet (HCCD); rats fed a hypercaloric choline-deficient diet supplemented with α-lipoic acid (HCCD+LA; consumed average daily dose of LA-61 mg/kg body weight). Values are mean ± standard error of the mean. p < 0.05 vs. * C; # HCCD+LA vs. HCCD.

Article Snippet: Animals were divided into three groups ( n = 8 in each): control pair-feeding group of rats fed a control diet (C) ( ); rats fed a hypercaloric choline-deficient diet (HCCD); rats fed a hypercaloric choline-deficient diet supplemented with α-lipoic acid (LA) (Chem Impex International, Inc., Wood Dale, USA, Cat# 29862) (HCCD+LA; consumed average daily dose of LA-61 mg/kg body weight).

Techniques: Activity Assay

Journal: Nutrients

Article Title: Lipoic Acid Exacerbates Oxidative Stress and Lipid Accumulation in the Liver of Wistar Rats Fed a Hypercaloric Choline-Deficient Diet

doi: 10.3390/nu13061999

Figure Lengend Snippet: Effects of α-lipoic acid (LA) supplementation on histological features of the liver (portal area, ×100): ( a ) control group, hematoxylin-eosin stain; ( b ) control group, van Gieson stain; ( c ) hypercaloric choline-deficient diet group, hematoxylin-eosin stain; ( d ) hypercaloric choline-deficient diet group, van Gieson stain; ( e ) hypercaloric choline-deficient diet supplemented with α-lipoic acid (consumed average daily dose of LA-61 mg/kg body weight), hematoxylin-eosin stain; ( f ) hypercaloric choline-deficient diet supplemented with α-lipoic acid (consumed average daily dose of LA-61 mg/kg body weight), van Gieson stain. Large-sized lipid droplets are indicated by the blue arrow, foci of lobular inflammation by the black arrow, and hepatocellular ballooning by the red arrow. Scale bar = 500 μm.

Article Snippet: Animals were divided into three groups ( n = 8 in each): control pair-feeding group of rats fed a control diet (C) ( ); rats fed a hypercaloric choline-deficient diet (HCCD); rats fed a hypercaloric choline-deficient diet supplemented with α-lipoic acid (LA) (Chem Impex International, Inc., Wood Dale, USA, Cat# 29862) (HCCD+LA; consumed average daily dose of LA-61 mg/kg body weight).

Techniques: Staining