Journal: Frontiers in Veterinary Science

Article Title: Development and evaluation of a fish feed mixture containing the probiotic Lactiplantibacillus plantarum prepared using an innovative pellet coating method

doi: 10.3389/fvets.2023.1196884

Figure Lengend Snippet: Viability of the L. plantarum R2 strain. The * symbol indicates the statistically significant differences between values. Number of stars indicate the level of significance (** p < 0.01, and *** p < 0.001).

Article Snippet: The viability of L. plantarum R2 Biocenol™ (CCM 8674) during an 11-month period in different storage temperatures (4°C, 22°C) was evaluated using the plate count method on MRS agar (Biolife, Italy).

Techniques:

Journal: Diseases

Article Title: Effects of Probiotics in Adults with Gastroenteritis: A Systematic Review and Meta-Analysis of Clinical Trials

doi: 10.3390/diseases11040138

Figure Lengend Snippet: Type of gastroenteritis, probiotics used and their effectiveness.

Article Snippet: Bjarnason, et al., 2019 [ ] , IBDs: UC ( n = 81) and Crohn’s disease (CD) ( n = 61); total samples more than 500. , Symprove contains multiple strains of probiotics such as Lactobacillus plantarum , Lactobacillus rhamnosus , Lactobacillus plantarum , Lactobacillus acidophilus , and E. faecium , Treatment , Yes , Score 4 out of 4 (High) , Multi-strain probiotics decreased intestinal inflammation in patients with UC, but not in patients with CD. , United Kingdom.

Techniques: Probiotics, Bacteria, Adjuvant, Control, Infection, Cell Culture

Journal:

Article Title: IS Lpl1 Is a Functional IS 30 -Related Insertion Element in Lactobacillus plantarum That Is Also Found in Other Lactic Acid Bacteria

doi: 10.1128/AEM.69.10.6032-6040.2003

Figure Lengend Snippet: IS Lp11 distribution in LAB

Article Snippet: The functionality of IS Lpl1 in other L. plantarum strains is suggested by the presence of up to 18 copies of iso-IS Lpl1 elements in different strains as well as by the variations in the IS Lpl1 patterns seen after 110 generations of strain NCIMB 1406 grown in MRS medium.

Techniques: Hybridization, Mutagenesis, Plasmid Preparation

Journal:

Article Title: IS Lpl1 Is a Functional IS 30 -Related Insertion Element in Lactobacillus plantarum That Is Also Found in Other Lactic Acid Bacteria

doi: 10.1128/AEM.69.10.6032-6040.2003

Figure Lengend Snippet: Simplified diagram of the metabolic pathways involved in the selection of the spontaneous mutant HN38. The intracellular UMP pool depends on pyrimidine synthesis and utilization of preformed pyrimidine supplied from RNA degradation or pumped from the culture medium. The upp gene catalyzed the key reaction in preformed uracil utilization (19). The UMP pool controls expression of the pyr genes involved in the de novo pyrimidine biosynthesis (shown with thick arrows). PyrR, an RNA binding protein, senses the concentration of UMP in the cell and regulates pyr gene expression through an attenuator mechanism. This regulation mechanism was demonstrated for Bacillus subtilis (24) and has been proposed for L. plantarum (11). Carbamoyl phosphate (CP) is a common intermediate for both arginine and pyrimidine biosynthesis. In L. plantarum, two CP synthetases (CPS) are present: a pyrimidine-regulated CPS encoded by the pyrAaAb genes (12) and an arginine-repressed CPS encoded by the carAB genes (20). In strain FB335, the carAB genes were deleted (20); growth relied on pyrAaAb expression to provide CP for both pathways. When uracil is provided, the pyr genes are not transcribed and FB335 cannot grow since it lacks CP for arginine biosynthesis. However, spontaneous uracil-resistant derivatives of FB335 were selected and one of these, mutant HN38, harbored the upp::ISLpl1 allele. HN38 grew in the presence of uracil, since the inactivation of upp may have generated a low UMP pool, which favored pyrAaAb expression.

Article Snippet: The functionality of IS Lpl1 in other L. plantarum strains is suggested by the presence of up to 18 copies of iso-IS Lpl1 elements in different strains as well as by the variations in the IS Lpl1 patterns seen after 110 generations of strain NCIMB 1406 grown in MRS medium.

Techniques: Selection, Mutagenesis, Expressing, RNA Binding Assay, Concentration Assay, Gene Expression, Generated

Journal:

Article Title: IS Lpl1 Is a Functional IS 30 -Related Insertion Element in Lactobacillus plantarum That Is Also Found in Other Lactic Acid Bacteria

doi: 10.1128/AEM.69.10.6032-6040.2003

Figure Lengend Snippet: ISLpl1-related element distribution in L. plantarum and Pediococcus strains. Southern hybridization blots with probe ISLpl1 (arrow b) or with probe Lambda DNA (arrow a). Lane 1, Lambda DNA digested with HindIII; lane 2, Lambda digested with HindIII and EcoRI. LAB genomes were digested with restriction enzymes HaeIII (lanes 3 to 8) and HindIII (lanes 9 to 15). Lanes 3 and 9, L. plantarum strain HN38; lanes 4 and 11, L. plantarum NCIMB 8826; lanes 5 and 13, L. plantarum ATCC 14917T; lanes 6 and 10, L. plantarum FB335; lanes 7 and 14, L. plantarum CNRZ 1891; lanes 8 and 15, L. plantarum NCFB 1406; lane 12, Pediococcus strain H.

Article Snippet: The functionality of IS Lpl1 in other L. plantarum strains is suggested by the presence of up to 18 copies of iso-IS Lpl1 elements in different strains as well as by the variations in the IS Lpl1 patterns seen after 110 generations of strain NCIMB 1406 grown in MRS medium.

Techniques: Hybridization, Lambda DNA Preparation

Journal:

Article Title: IS Lpl1 Is a Functional IS 30 -Related Insertion Element in Lactobacillus plantarum That Is Also Found in Other Lactic Acid Bacteria

doi: 10.1128/AEM.69.10.6032-6040.2003

Figure Lengend Snippet: Plasmid and chromosomal localization of ISLpl1. (A) Ethidium bromide-stained DNA after agarose gel electrophoresis; (B) Southern blot DNA bands hybridized with the digoxigenin-labeled specific ISLpl1 probe. Lanes 1, 3, and 5, native plasmid DNA extracted from L. plantarum strains FB335, NCIMB 1406, and NCIMB 8826, respectively; lanes 2 and 4, HindIII-digested CsCl gradient linear DNA preparations of strain FB335 and NCIMB 1406, respectively. Linear DNA molecular mass markers (Raoul marker; Appligene) are indicated in kilobases. Native plasmid band sizes were deduced from the three plasmids harbored by strain NCIMB 8826 (36, 2.3, and 1.9 kb). Native plasmid bands detected by the IS probe are shown by circles representing strains FB335 (circle in panel B, lane 1) and NCIMB 1406 (circles in panel B, lane 3).

Article Snippet: The functionality of IS Lpl1 in other L. plantarum strains is suggested by the presence of up to 18 copies of iso-IS Lpl1 elements in different strains as well as by the variations in the IS Lpl1 patterns seen after 110 generations of strain NCIMB 1406 grown in MRS medium.

Techniques: Plasmid Preparation, Staining, Agarose Gel Electrophoresis, Southern Blot, Labeling, Marker

Journal:

Article Title: IS Lpl1 Is a Functional IS 30 -Related Insertion Element in Lactobacillus plantarum That Is Also Found in Other Lactic Acid Bacteria

doi: 10.1128/AEM.69.10.6032-6040.2003

Figure Lengend Snippet: Stability over time of the ISLpl1-related element patterns in L. plantarum strain NCIMB 1406. Insertion sites were evaluated with ISLpl1 DNA probed against HaeIII digests of NCIMB 1406-derived strains, using Southern hybridization under low-stringency conditions. The IS profile was analyzed in four clones isolated after 110 generations of NCIMB 1406 grown in liquid MRS medium. Arrows indicate the 18 bands detected in strain NCIMB 1406 at the beginning of the experiment. Clones 1 to 4 were isolated from a liquid MRS culture obtained by serial cultivation of strain NCIMB 1406 as described in Materials and Methods. Circles show detected bands, which differ from those of the parent strain. (A) DNA after agarose gel electrophoresis. (B) Southern blot. MW marker, molecular mass marker (Raoul marker; Appligene) bands (indicated in kilobases).

Article Snippet: The functionality of IS Lpl1 in other L. plantarum strains is suggested by the presence of up to 18 copies of iso-IS Lpl1 elements in different strains as well as by the variations in the IS Lpl1 patterns seen after 110 generations of strain NCIMB 1406 grown in MRS medium.

Techniques: Derivative Assay, Hybridization, Clone Assay, Isolation, Agarose Gel Electrophoresis, Southern Blot, Marker

Journal: Engineering in Life Sciences

Article Title: Gut microbiota and childhood malnutrition: Understanding the link and exploring therapeutic interventions

doi: 10.1002/elsc.202300070

Figure Lengend Snippet: Some studies on childhood malnutrition using probiotics, prebiotics and synbiotics.

Article Snippet: According to the animal study reported by Schwarzer et al., the peptidoglycans of L. plantarum LpWJL strain can activate anti‐stunting and growth promoting responses by inducing the biological function of the intestinal nucleotide‐binding oligomerization domain 2 receptor [ ].

Techniques: Probiotics, Concentration Assay, Bacteria, Infection, Starch