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mouse tumor derived kras mutant cell line mt4  (Cold Spring Harbor Laboratory Meetings)
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Cold Spring Harbor Laboratory Meetings mouse tumor derived kras mutant cell line mt4
Dual inhibition of IGF1R and autophagy impairs PDAC proliferation in 3D and in vivo models. A, Human (Pa01C, Pa14C) and murine <t>(mT4)</t> PDAC cell lines were grown as spheroids and treated with or without BMS-754807 (IGF1Ri, 200 nM) and/or CQ (6 μM) for 72 hours and cytotoxicity was assayed via the addition of the apoptotic dye CellTox Green (500 nM). Representative images at 0 and 72 hours of drug treatment are shown (top). Scale bar = 400 μm. Total integrated intensity of CellTox fluorescence at 72 hours after treatment is plotted (bottom). Integrated CellTox fluorescence intensity is representative of at least four independent experiments. B, Ex vivo tumor weight of mT4 mouse xenografts following treatment with vehicle, BMS-754807 (IGF1Ri, 12.5 mg/kg), HCQ (60 mg/kg), and the combination. C, GSEA of Hallmark gene sets following Clariom S Assay HT mouse microarray of mRNA prepared from six representative mT4 tumors per condition from B .
Mouse Tumor Derived Kras Mutant Cell Line Mt4, supplied by Cold Spring Harbor Laboratory Meetings, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Dual inhibition of IGF1R and autophagy impairs PDAC proliferation in 3D and in vivo models. A, Human (Pa01C, Pa14C) and murine (mT4) PDAC cell lines were grown as spheroids and treated with or without BMS-754807 (IGF1Ri, 200 nM) and/or CQ (6 μM) for 72 hours and cytotoxicity was assayed via the addition of the apoptotic dye CellTox Green (500 nM). Representative images at 0 and 72 hours of drug treatment are shown (top). Scale bar = 400 μm. Total integrated intensity of CellTox fluorescence at 72 hours after treatment is plotted (bottom). Integrated CellTox fluorescence intensity is representative of at least four independent experiments. B, Ex vivo tumor weight of mT4 mouse xenografts following treatment with vehicle, BMS-754807 (IGF1Ri, 12.5 mg/kg), HCQ (60 mg/kg), and the combination. C, GSEA of Hallmark gene sets following Clariom S Assay HT mouse microarray of mRNA prepared from six representative mT4 tumors per condition from B .

Journal: Cancer research

Article Title: Concurrent inhibition of IGF1R and ERK increases pancreatic cancer sensitivity to autophagy inhibitors

doi: 10.1158/0008-5472.CAN-21-1443

Figure Lengend Snippet: Dual inhibition of IGF1R and autophagy impairs PDAC proliferation in 3D and in vivo models. A, Human (Pa01C, Pa14C) and murine (mT4) PDAC cell lines were grown as spheroids and treated with or without BMS-754807 (IGF1Ri, 200 nM) and/or CQ (6 μM) for 72 hours and cytotoxicity was assayed via the addition of the apoptotic dye CellTox Green (500 nM). Representative images at 0 and 72 hours of drug treatment are shown (top). Scale bar = 400 μm. Total integrated intensity of CellTox fluorescence at 72 hours after treatment is plotted (bottom). Integrated CellTox fluorescence intensity is representative of at least four independent experiments. B, Ex vivo tumor weight of mT4 mouse xenografts following treatment with vehicle, BMS-754807 (IGF1Ri, 12.5 mg/kg), HCQ (60 mg/kg), and the combination. C, GSEA of Hallmark gene sets following Clariom S Assay HT mouse microarray of mRNA prepared from six representative mT4 tumors per condition from B .

Article Snippet: The mouse tumor-derived Kras -mutant cell line mT4 [KPC ( Kras G12D/+ ; Tp53 R172H/+ ; Pdx-Cre)] and the KRAS WT hF39 and hF43 cell lines were provided by D. Tuveson (Cold Spring Harbor Laboratory, NY).

Techniques: Inhibition, In Vivo, CellTox Assay, Fluorescence, Ex Vivo, Microarray