Journal: Frontiers in Neuroscience

Article Title: Imaging stretch-activated firing of spinal afferent nerve endings in mouse colon

doi: 10.3389/fnins.2013.00179

Figure Lengend Snippet: TRPV1 immunoreactive nerve endings identified in mouse colonic myenteric ganglia and circular smooth muscle. (A–C) Show dense TRPV1 varicose fibers innervate myenteric ganglia and circular muscle. The arrow in (A) shows the ending of a varicose TRPV1 positive nerve ending that lacks complex morphological specialization. (D) Shows a higher magnification (40× water immersion lens) of the dense TRPV1 positive varicose axons that ramify within myenteric ganglia.

Article Snippet: Specimens were labeled by incubation with antisera to TRPV1 (VR1) sourced from Alomone Labs, Product #: ACC-030 for 2 days at a concentration of 1:500, then washed (10 min immersion, repeated three times) in PBS.

Techniques:

Journal: Journal of Gastroenterology

Article Title: TRPV6 -related pancreatitis: natural history and the impact of the pancreas-specific deletion on pancreatitis in mice

doi: 10.1007/s00535-025-02323-y

Figure Lengend Snippet: Non-synonymous TRPV6 variants detected in patients in this study

Article Snippet: Slides were boiled in target retrieval solution (Dako, Glostrup, Denmark) and incubated with rabbit anti-TRPV6 antibody (ACC-036; Alomone Labs, Jerusalem, Israel) overnight at 4 °C.

Techniques:

Journal: Journal of Gastroenterology

Article Title: TRPV6 -related pancreatitis: natural history and the impact of the pancreas-specific deletion on pancreatitis in mice

doi: 10.1007/s00535-025-02323-y

Figure Lengend Snippet: Predicted structures of the mutated TRPV6 protein based on the minigene assays. Normal splicing of the wild-type sequence and aberrant splicing of the mutant sequence are illustrated for each variant

Article Snippet: Slides were boiled in target retrieval solution (Dako, Glostrup, Denmark) and incubated with rabbit anti-TRPV6 antibody (ACC-036; Alomone Labs, Jerusalem, Israel) overnight at 4 °C.

Techniques: Sequencing, Mutagenesis, Variant Assay

Journal: Journal of Gastroenterology

Article Title: TRPV6 -related pancreatitis: natural history and the impact of the pancreas-specific deletion on pancreatitis in mice

doi: 10.1007/s00535-025-02323-y

Figure Lengend Snippet: Clinical characteristics of enrolled patients

Article Snippet: Slides were boiled in target retrieval solution (Dako, Glostrup, Denmark) and incubated with rabbit anti-TRPV6 antibody (ACC-036; Alomone Labs, Jerusalem, Israel) overnight at 4 °C.

Techniques:

Journal: Journal of Gastroenterology

Article Title: TRPV6 -related pancreatitis: natural history and the impact of the pancreas-specific deletion on pancreatitis in mice

doi: 10.1007/s00535-025-02323-y

Figure Lengend Snippet: Comparison of different pathogenic genotypes on clinical outcomes. Kaplan–Meier curves showing the cumulative rates of A symptom onset, B pancreatic calcification, C pancreatic exocrine insufficiency, and D diabetes mellitus, according to genotype ( TRPV6 -related, PRSS1 -related, SPINK1 -related, or PV-negative pancreatitis). Censored subjects are indicated on the Kaplan–Meier curves by tick marks

Article Snippet: Slides were boiled in target retrieval solution (Dako, Glostrup, Denmark) and incubated with rabbit anti-TRPV6 antibody (ACC-036; Alomone Labs, Jerusalem, Israel) overnight at 4 °C.

Techniques: Comparison

Journal: Journal of Gastroenterology

Article Title: TRPV6 -related pancreatitis: natural history and the impact of the pancreas-specific deletion on pancreatitis in mice

doi: 10.1007/s00535-025-02323-y

Figure Lengend Snippet: Timing of the first interventions for pancreatitis and the diagnosis of pancreatic cancer according to pathogenic genotypes. Kaplan–Meier curves showing the cumulative rates of A endoscopic treatment, B surgery, C all interventions, and D pancreatic cancer diagnosis according to genotype ( TRPV6 -related, PRSS1 -related, SPINK1 -related, or PV-negative pancreatitis). Censored subjects are indicated on the Kaplan–Meier curves by tick marks

Article Snippet: Slides were boiled in target retrieval solution (Dako, Glostrup, Denmark) and incubated with rabbit anti-TRPV6 antibody (ACC-036; Alomone Labs, Jerusalem, Israel) overnight at 4 °C.

Techniques: Biomarker Discovery

Journal: Journal of Gastroenterology

Article Title: TRPV6 -related pancreatitis: natural history and the impact of the pancreas-specific deletion on pancreatitis in mice

doi: 10.1007/s00535-025-02323-y

Figure Lengend Snippet: Caerulein-induced pancreatitis was exacerbated in pancreas-specific Trpv6 conditional knockout mice. A – D Trpv6 floxed mice and pancreas-specific Trpv6 CKO mice received eight hourly intraperitoneal injections of caerulein (100 μg/kg body weight) or saline for two consecutive days. Blood samples were collected at 0 h (just before the first caerulein injection), 8 h, 24 h, and 32 h, and mice were euthanized at either 32 h or 96 h. A Scheme of the experiments. B Blood samples were obtained at the indicated time points, and serum amylase levels were measured. C Representative H&E staining of the pancreas at 32 h. Scale bar = 100 μm. D Representative H&E staining and Sirius Red staining of the pancreas at 96 h after the first caerulein injection. E , F CP was induced by 6-hourly intraperitoneal injections of caerulein (100 μg/kg body weight), 3 days/week for four consecutive weeks. E Scheme of the experiments. F Representative H&E staining and Sirius Red staining of the pancreas after 4 weeks

Article Snippet: Slides were boiled in target retrieval solution (Dako, Glostrup, Denmark) and incubated with rabbit anti-TRPV6 antibody (ACC-036; Alomone Labs, Jerusalem, Israel) overnight at 4 °C.

Techniques: Gene Knockout, Saline, Blood/Plasma/Serum Samples, Injection, Staining, H&E Stain