Journal: In Vivo

Article Title: Distinct Expression of Surface and Genetic Biomarkers in Prostate Cancer Cell Lines

doi: 10.21873/invivo.13073

Figure Lengend Snippet: PCA3: Prostate cancer antigen 3; KLK4: kallikrein 4; KLK9: kallikrein 9.

Article Snippet: TaqMan ® probes purchased from Applied Biosystems were used to measure the target genes, and probes used in this study included b-actin (Hs99999903_m1), prostate cancer antigen 3 (PCA3, Hs01371939_g1), kallikrein related peptidase 4 (KLK4, Hs00191772_m1), and KLK9 (Hs01043981_m1).

Techniques:

Journal: British Journal of Pharmacology

Article Title: Newly developed serine protease inhibitors decrease visceral hypersensitivity in a post‐inflammatory rat model for irritable bowel syndrome

doi: 10.1111/bph.14396

Figure Lengend Snippet: TaqMan primers used for qPCR analysis of colonic and DRG samples

Article Snippet: Protein Gene ID Tryptase αβ1 Rn00570928_m1 Matriptase Rn00586242_m1 Cathepsin G Rn01489144_g1 Urokinase plasminogen activator Rn00565261_m1 KLK2 Rn00820615_m1 KLK4 Rn01498534_g1 KLK8 Rn01476995_m1 PAR2 Rn00588089_m1 PAR4 Rn00587480_m1 TRPA1 Rn01473803_m1 TRPV1 Rn00583117_m1 TRPV4 Rn00576745_m1 GAPDH Rn01775763_g1 β‐actin Rn00667869_m1 Open in a separate window TaqMan primers used for qPCR analysis of colonic and DRG samples Table A2 Proteases UAMC‐00050 UAMC‐01162 Nafamostat mesilate IC 50 (μM) IC 50 (μM) Est.

Techniques:

Journal: British Journal of Pharmacology

Article Title: Newly developed serine protease inhibitors decrease visceral hypersensitivity in a post‐inflammatory rat model for irritable bowel syndrome

doi: 10.1111/bph.14396

Figure Lengend Snippet: Inhibitory profiles for the serine protease inhibitors UAMC‐00050, UAMC‐01162 and nafamostat mesilate

Article Snippet: Protein Gene ID Tryptase αβ1 Rn00570928_m1 Matriptase Rn00586242_m1 Cathepsin G Rn01489144_g1 Urokinase plasminogen activator Rn00565261_m1 KLK2 Rn00820615_m1 KLK4 Rn01498534_g1 KLK8 Rn01476995_m1 PAR2 Rn00588089_m1 PAR4 Rn00587480_m1 TRPA1 Rn01473803_m1 TRPV1 Rn00583117_m1 TRPV4 Rn00576745_m1 GAPDH Rn01775763_g1 β‐actin Rn00667869_m1 Open in a separate window TaqMan primers used for qPCR analysis of colonic and DRG samples Table A2 Proteases UAMC‐00050 UAMC‐01162 Nafamostat mesilate IC 50 (μM) IC 50 (μM) Est.

Techniques: Clinical Proteomics

Journal: British Journal of Pharmacology

Article Title: Newly developed serine protease inhibitors decrease visceral hypersensitivity in a post‐inflammatory rat model for irritable bowel syndrome

doi: 10.1111/bph.14396

Figure Lengend Snippet: Relative mRNA expression of serine proteases in colon samples

Article Snippet: Protein Gene ID Tryptase αβ1 Rn00570928_m1 Matriptase Rn00586242_m1 Cathepsin G Rn01489144_g1 Urokinase plasminogen activator Rn00565261_m1 KLK2 Rn00820615_m1 KLK4 Rn01498534_g1 KLK8 Rn01476995_m1 PAR2 Rn00588089_m1 PAR4 Rn00587480_m1 TRPA1 Rn01473803_m1 TRPV1 Rn00583117_m1 TRPV4 Rn00576745_m1 GAPDH Rn01775763_g1 β‐actin Rn00667869_m1 Open in a separate window TaqMan primers used for qPCR analysis of colonic and DRG samples Table A2 Proteases UAMC‐00050 UAMC‐01162 Nafamostat mesilate IC 50 (μM) IC 50 (μM) Est.

Techniques: Expressing, Control

Journal: PLoS ONE

Article Title: Comparative Transcriptome Analysis of Fetal Skin Reveals Key Genes Related to Hair Follicle Morphogenesis in Cashmere Goats

doi: 10.1371/journal.pone.0151118

Figure Lengend Snippet: (a) E60: KLK11 was detected in the epidermis. The placode of HF shows little or no immunoreactivity at this stage (arrow). (b) E120: KLK11 was expressed strongly in the root sheaths and slightly in the bulb of HF (arrow). (c) NB: KLK11 was present in the hair matrix cells and root sheaths (arrow). (d) E60: VDR shows intense immunostaining in the epidermis. Very little VDR immunoreactivity was detected in the placode (arrow). (e) E120: VDR immunoreactivity is predominantly detected in the ORS keratinocytes and the bulb of HF (arrow). (f) NB: In fully formed HF, VDR staining was present in the root sheaths and bulb cells, where immunoreactivity was particularly enhanced in the ORS keratinocyte and matrix zone. Bar: 100 μm.

Article Snippet: They were then incubated at 4°C overnight in a humidified chamber with the following primary antibodies: VDR (vitamin D receptor) (rabbit, 1:300; Boster, Wuhan, Hubei, China), KLK11 (kallikrein 11) (rabbit, 1:100; Boster, Wuhan, Hubei, China).

Techniques: Immunostaining, Staining

Journal: PLoS ONE

Article Title: Characterization of kallikrein-related peptidase 4 (KLK4) mRNA expression in tumor tissue of advanced high-grade serous ovarian cancer patients

doi: 10.1371/journal.pone.0212968

Figure Lengend Snippet: The cumulative histogram depicts relative KLK4 mRNA expression levels (normalized to HPRT mRNA levels). Most samples display very low expression. For further analysis, we categorized the KLK4 mRNA expression levels into low expression versus high expression groups by the 50 th percentile (median).

Article Snippet: For KLK4, the assay was optimized to use a TaqMan gene expression assay from ThermoFisher for characterizing KLK4 mRNA expression (assay ID: Hs05041835_s1).

Techniques: Expressing

Journal: PLoS ONE

Article Title: Characterization of kallikrein-related peptidase 4 (KLK4) mRNA expression in tumor tissue of advanced high-grade serous ovarian cancer patients

doi: 10.1371/journal.pone.0212968

Figure Lengend Snippet: Association between KLK4 mRNA expression levels and clinical characteristics in patients with advanced high-grade serous ovarian cancer (FIGO stage III/IV).

Article Snippet: For KLK4, the assay was optimized to use a TaqMan gene expression assay from ThermoFisher for characterizing KLK4 mRNA expression (assay ID: Hs05041835_s1).

Techniques: Expressing

Journal: PLoS ONE

Article Title: Characterization of kallikrein-related peptidase 4 (KLK4) mRNA expression in tumor tissue of advanced high-grade serous ovarian cancer patients

doi: 10.1371/journal.pone.0212968

Figure Lengend Snippet: Univariate Cox regression analysis of KLK4 mRNA expression levels and patients’ survival in advanced high-grade ovarian cancer (FIGO III/IV).

Article Snippet: For KLK4, the assay was optimized to use a TaqMan gene expression assay from ThermoFisher for characterizing KLK4 mRNA expression (assay ID: Hs05041835_s1).

Techniques: Expressing

Journal: PLoS ONE

Article Title: Characterization of kallikrein-related peptidase 4 (KLK4) mRNA expression in tumor tissue of advanced high-grade serous ovarian cancer patients

doi: 10.1371/journal.pone.0212968

Figure Lengend Snippet: Patients with high KLK4 mRNA expression levels display a significantly worse OS rate (Kaplan-Meier analysis, p = 0.001) (A) but not PFS (B), compared to patients with low KLK4 mRNA expression levels.

Article Snippet: For KLK4, the assay was optimized to use a TaqMan gene expression assay from ThermoFisher for characterizing KLK4 mRNA expression (assay ID: Hs05041835_s1).

Techniques: Expressing

Journal: PLoS ONE

Article Title: Characterization of kallikrein-related peptidase 4 (KLK4) mRNA expression in tumor tissue of advanced high-grade serous ovarian cancer patients

doi: 10.1371/journal.pone.0212968

Figure Lengend Snippet: The microarray data set analyzed for KLK4 mRNA expression (probe ID 1555737_a_at) was from The Cancer Genome Atlas (TCGA). The patients were selected for serous histological type, advanced stage (FIGO III/IV), high-grade (grade 3), platinum-containing chemotherapy, and 5 years’ follow-up. This selection resulted in a patient cohort encompassing 252 patients for analysis of the association with OS (A) and 249 patients for PFS (B), respectively.

Article Snippet: For KLK4, the assay was optimized to use a TaqMan gene expression assay from ThermoFisher for characterizing KLK4 mRNA expression (assay ID: Hs05041835_s1).

Techniques: Microarray, Expressing, Selection

Journal: PLoS ONE

Article Title: Characterization of kallikrein-related peptidase 4 (KLK4) mRNA expression in tumor tissue of advanced high-grade serous ovarian cancer patients

doi: 10.1371/journal.pone.0212968

Figure Lengend Snippet: Multivariable Cox regression analysis of KLK4 mRNA expression levels and patients survival in advanced high-grade ovarian cancer (FIGO III/IV).

Article Snippet: For KLK4, the assay was optimized to use a TaqMan gene expression assay from ThermoFisher for characterizing KLK4 mRNA expression (assay ID: Hs05041835_s1).

Techniques: Expressing

Journal: Scientific Reports

Article Title: Identification of the C-terminal region in Amelogenesis Imperfecta causative protein WDR72 required for Golgi localization

doi: 10.1038/s41598-022-08719-7

Figure Lengend Snippet: Expression of Wdr72 in ALC and LS8 cells at RNA and protein levels. ( A ) Gene expression of Wdr72 . The expression levels of amelogenesis gene markers at maturation stage ( Odam , Odaph and Klk4 ) and those at secretory stage ( Mmp20 , Amelx and Enam ) were measured, normalized to that of β-actin and compared between ALC and LS8 cells. The normalized value in ALC cells in each gene expression was used as a calibrator. The values are shown as the mean ± SD based on the averages of three independent experiments with triplicates in each. The values in each marker were compared between ALC and LS8 and statistically analyzed by student T-test. An asterisk represents a statistically significant difference between the values of ALC and LS8 in each marker ( p < 0.01). ( B ) Protein expression of WDR72. Western blot analysis (WB) was performed using ALC, LS8 and NIH3T3 cell extracts to investigate the expression of WDR72 protein by anti-WDR72 antibody. The HEK293 cells were transiently transfected with Flag-tagged human WDR72 wild-type expression vector or an empty vector, and the lysates were used as a positive control (Lane 4, Transfected WDR72) and a negative control for transfection (Lane 5, Control). Western blot analysis by anti-Flag antibody was also performed to confirm the presence of Flag-WDR72 protein. The expression of β-actin was shown as a loading control. The experiment was repeated three times and representative images are shown.

Article Snippet: The following markers were used: Wdr72 , Odam (Odontogenic, ameloblast associated, Mm02581573_m1), Odaph (Odontogenesis associated phosphoprotein, Mm02020633_s1), Klk4 (Kallikrein related peptidase 4, Mm00517341_m1), Mmp20 (Matrix Metallopeptidase-20, Mm00600244_m1), Amelx (Amelogenin X-linked, Mm00711642_m1) and Enam (Enamelin, Mm00516922_m1).

Techniques: Expressing, Gene Expression, Marker, Western Blot, Transfection, Plasmid Preparation, Positive Control, Negative Control, Control

Journal: Frontiers in Pharmacology

Article Title: Three-Dimensional Culture of Ameloblast-Originated HAT-7 Cells for Functional Modeling of Defective Tooth Enamel Formation

doi: 10.3389/fphar.2021.682654

Figure Lengend Snippet: Quantitative RT-PCR data showing expression of: (A) maturation-phase ameloblast marker Klk4, and tight-junction protein genes Cldn1, Cldn4, Cldn8 (claudins 1, 4, and 8), and Tjp1 (ZO-1); (B) electrolyte transporter genes Slc9a1 (NHE1), Slc4a2 (AE2), Slc4a4 (NBCe1), Slc26a4 (pendrin), and Cftr (CFTR). Expression levels in HAT-7 cells grown in Matrigel with Hepato-STIM medium (spheroid) were normalised to expression levels in HAT-7 cells grown on Transwell permeable supports in differentiation medium (monolayer). Data are mean ± SEM. * p < 0.05 (n = 4).

Article Snippet: Amplification was performed using QuantStudio 5 with TaqPath qPCR Master Mix, CG (Applied Biosystems, Foster City, CA, United States) and predesigned primers (Life Technologies Magyarország Kft., Budapest): Klk4 (Rn01498536_m1), Cldn1 (Rn00581740_m1), Cldn4 (Rn01196224_s1), Cldn8 (Rn01767199_s1), Tjp1/ZO-1 (Rn02116071_s1), Slc4a2/AE2 (Rn00566910_m1), Slc4a4/NBCe1 (Rn00584747_m1), Slc9a1/NHE1 (Rn00561924_m1), CFTR (Rn01455971_m1), Slc26a4/pendrin (Rn00693043_m1).

Techniques: Quantitative RT-PCR, Expressing, Marker

Journal: Iranian Journal of Pathology

Article Title: Expression of Human Kallikreins 4, 8, 10, 11 and 13 in Pleomorphic Adenomas and Mucoepidermoid Carcinomas

doi:

Figure Lengend Snippet: KLK13, 4, 8, 10, 11staining in Mucoepidermoid carcinoma (Average staining ± SD

Article Snippet: Appropriate positive and negative (tissue slides with primary antibodies omitted) controls were used (Human KLK-8/Kallikrein-8 Human Cells Transfected Lysate (positive control), Human KLK-4/ Kallikrein-4 Human Cells Transfected Lysate (positive control), Kallikrein 13 transfected 293T cell lysat, Lysate from 293T cells transfected with Kallikrein 10 and KLK11 / Kallikrein-11 Human Cells Transfected Lysate) (Sino Biological Inc, China and Asia Chemi Tab Co, Tehran, Iran).

Techniques: Staining