Journal: Nature medicine

Article Title: Gut Microbial Metabolism of 5-ASA Diminishes Its Clinical Efficacy in Inflammatory Bowel Disease

doi: 10.1038/s41591-023-02217-7

Figure Lengend Snippet: (A) In vitro competition assay with 1 mM of 5-ASA, 4-ASA, procainamide, hydralazine and isoniazide demonstrates relative specificity of the Firmicutes CAG:176 thiolase (FcTHL) for 5-ASA. N=3 biologically independent samples per enzyme/condition. (B) Shown is a representative Michaelis-Menten plot of n=1 thiolase enzyme preparation, conducted in technical triplicates at each concentration of 5-ASA; summary data is from n=5 biologically independent experiments each conducted in technical triplicate. (C) Live culture of Oscillibacter sp., strain KLE 1745 encoding another predicted thiolase gene (UniRef90 R6TIX3) was capable of acetylation of 5-ASA to N-acetyl 5-ASA. N=3 biologically independent samples per condition, representative data from n=2 independent experiments, unpaired, two-sided T-test, *** = p = 0.0015. Data are presented as mean values +/− SEM.

Article Snippet: Oscillibacter sp., strain KLE 1745 was obtained through BEI resources, the NIH Institute of Allergy and Infectious Diseases (NIAID) as part of the Human Microbiome Project.

Techniques: In Vitro, Competitive Binding Assay, Concentration Assay

Journal: Molecular Medicine

Article Title: Celastrol promotes DNA damage and apoptosis in uterine corpus endometrial carcinoma via promotion of KAT2B-mediated RBPJ acetylation and repression of MCM4 transcription

doi: 10.1186/s10020-025-01082-z

Figure Lengend Snippet: Celastrol suppresses cell proliferation in UCEC. Four UCEC cell lines Ishikawa, HEC-1-B, KLE and HEC-1-A were applied and treated with different concentrations of Celastrol (0, 0.3, 0.625, 1.25, 2.5, 5, 10 or 20 µM) for 24 h. ( A ) The chemical structure of Celastrol. ( B ) CCK8 assay tested the cell viability, and cell inhibition rate (CI) was calculated. ( C ) Ishikawa and HEC-1-A cells were treated with 2.5, 5 or 10 µM of Celastrol (L-Celastrol, M-Celastrol or H-Celastrol) and cultured for 24 h. The distribution of cell cycle was evaluated by flow cytometry analysis. ( D ) Cell cycle-associated protein expression levels were measured by western blotting assays. All the values are mean ± SD. ∗ P < 0.05, ∗∗ P < 0.01 vs. the control group

Article Snippet: The human endometrial cancer cell lines Ishikawa, HEC-1-B, KLE and HEC-1-A were obtained from iCell Bioscience Inc (Shanghai, China).

Techniques: CCK-8 Assay, Inhibition, Cell Culture, Flow Cytometry, Expressing, Western Blot, Control

Journal: Oncology Letters

Article Title: Expression of tumor suppressor programmed cell death 4 in endometrioid endometrial carcinomas and clinicopathological significance

doi: 10.3892/ol.2018.8517

Figure Lengend Snippet: Representative images of immunocytochemical analysis of PDCD4 in control endometrial glandular epithelial cells and KLE cells (×400 magnification; scale bars=20 µm). (A) Immunocytochemical staining of cytokeratin in the glandular epithelial cells of control endometrium. (B) Immunocytochemical staining of vimentin in the stromal cells of control endometrium. (C) Immunocytochemical staining of PDCD4 in the glandular epithelial cells of control endometrium. (D) Immunocytochemical staining of PDCD4 in KLE cells. PDCD4, programmed cell death 4.

Article Snippet: KLE cells (G3 endometrioid endometrial carcinoma cells) were purchased from China Center for Type Culture Collection (Wuhan, China).

Techniques: Control, Staining