Journal: Scientific Reports

Article Title: MBSP1: a biosurfactant protein derived from a metagenomic library with activity in oil degradation

doi: 10.1038/s41598-020-58330-x

Figure Lengend Snippet: Prediction of intrinsic disorder by PONDR tool. The predicted degree of disorder tendency (scale 0–1; cut-off 0.5) is charted over the amino acid positions within 3C6 ORF (MBSP1). The prediction tool indicates a mostly disordered protein structure. Red bars at the top of the diagram indicate the positions of disordered prediction in the structure.

Article Snippet: Disorder prediction using the Predictor of Natural Disordered Regions (PONDR®) tool (Molecular Kinetics, Inc., Indianapolis, IN, USA) showed 131 disordered residues, corresponding to 43.96% of the 3C6 entire structure.

Techniques:

Journal: Blood Advances

Article Title: Calcium-RasGRP2-Rap1 signaling mediates CD38-induced migration of chronic lymphocytic leukemia cells

doi: 10.1182/bloodadvances.2017014506

Figure Lengend Snippet: CD38 expression is associated with elevated basal Ca2+levels. MEC1-GFP, MEC1-CD38H, and primary CLL cells with varying CD38 expression levels (n = 17 patient samples, analyzed in 3 independent experiments) were incubated with the ratiometric Ca2+ indicator Indo-1 and analyzed by flow cytometry. The basal fluorescence level was recorded for 60 seconds before stimulation with CCL21 (100 ng/mL) for a further 120 seconds. (A,C) Representative bivariate plot of the median of fluorescent intensity ratio (Indo-1 emission wavelengths: 420/510 nm) against time; red boxes indicate the basal fluorescence level. (B,D) Peak values of the median basal fluorescence intensity were obtained using FlowJo Kinetic statistic tool. Horizontal bars in panel D indicate mean values of the fluorescence intensity peak calculated in each experiment for CD38low (green) and CD38high (red) samples. Significant differences between the 2 experimental groups were determined using a 2-tailed Student t test.

Article Snippet: The basal fluorescence level was recorded for 60 seconds before stimulation with CCL21 (100 ng/mL) for a further 120 seconds. (A,C) Representative bivariate plot of the median of fluorescent intensity ratio (Indo-1 emission wavelengths: 420/510 nm) against time; red boxes indicate the basal fluorescence level. (B,D) Peak values of the median basal fluorescence intensity were obtained using FlowJo Kinetic statistic tool.

Techniques: Expressing, Incubation, Flow Cytometry, Fluorescence