kinase Search Results


t4 polynucleotide kinase  (New England Biolabs)
99
New England Biolabs t4 polynucleotide kinase
T4 Polynucleotide Kinase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/t4 polynucleotide kinase/product/New England Biolabs
Average 99 stars, based on 1 article reviews
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universal tyrosine kinase assay kit  (TaKaRa)
94
TaKaRa universal tyrosine kinase assay kit
Universal Tyrosine Kinase Assay Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/universal tyrosine kinase assay kit/product/TaKaRa
Average 94 stars, based on 1 article reviews
universal tyrosine kinase assay kit - by Bioz Stars, 2026-03
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anti p70s6k  (Cell Signaling Technology Inc)
99
Cell Signaling Technology Inc anti p70s6k
Anti P70s6k, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti p70s6k/product/Cell Signaling Technology Inc
Average 99 stars, based on 1 article reviews
anti p70s6k - by Bioz Stars, 2026-03
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rabbit anti cjun n terminal kinase jnk antibody  (Proteintech)
96
Proteintech rabbit anti cjun n terminal kinase jnk antibody
Rabbit Anti Cjun N Terminal Kinase Jnk Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti cjun n terminal kinase jnk antibody/product/Proteintech
Average 96 stars, based on 1 article reviews
rabbit anti cjun n terminal kinase jnk antibody - by Bioz Stars, 2026-03
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nf κb p65  (Proteintech)
96
Proteintech nf κb p65
Fig. 4 Effect of hawthorn leather treatment on expression levels of inflammatory cytokines <t>and</t> <t>NF-κB</t> signaling pathway in the liver tissue of mice. mRNA expression level of (A) IL-1β, (B) Nos2, (C) Cox2, (D) TLR4, (E) MyD88, and (F) NF-κB; (G) Images of western blotting; Protein expression ratio of (H) MyD88 to GAPDH and (I) NF-κB to GAPDH. Data are expressed as mean ± SD (8 mice/group). Values of each group with different letters over the bars are significantly different (P < 0.05) by analysis of one-way ANOVA followed by Tukey’s multiple range test.
Nf κb P65, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nf κb p65/product/Proteintech
Average 96 stars, based on 1 article reviews
nf κb p65 - by Bioz Stars, 2026-03
96/100 stars
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akt  (Proteintech)
96
Proteintech akt
Fig. 4 Effect of hawthorn leather treatment on expression levels of inflammatory cytokines <t>and</t> <t>NF-κB</t> signaling pathway in the liver tissue of mice. mRNA expression level of (A) IL-1β, (B) Nos2, (C) Cox2, (D) TLR4, (E) MyD88, and (F) NF-κB; (G) Images of western blotting; Protein expression ratio of (H) MyD88 to GAPDH and (I) NF-κB to GAPDH. Data are expressed as mean ± SD (8 mice/group). Values of each group with different letters over the bars are significantly different (P < 0.05) by analysis of one-way ANOVA followed by Tukey’s multiple range test.
Akt, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/akt/product/Proteintech
Average 96 stars, based on 1 article reviews
akt - by Bioz Stars, 2026-03
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rage  (Proteintech)
93
Proteintech rage
Fig. 7. CSP supplementation reduced D-gal-induced overexpression of <t>RAGE,</t> BACE-1, Aβ-42 <t>and</t> <t>PS1.</t> (A) Western blot analysis of RAGE, BACE-1, Aβ-42 and PS1; (B) The relative protein expressions of RAGE, BACE-1, Aβ-42 and PS1; Data are expressed as the mean ± SEM (n = 3). Differences were denoted as follows: * P < 0.05, ** P < 0.01, *** P < 0.001 compared with NC; # P < 0.05, ## p < 0.01, ### p < 0.001 compared with D-gal; & P < 0.05, && p < 0.01, &&& p < 0.001 compared with CSPL.
Rage, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rage/product/Proteintech
Average 93 stars, based on 1 article reviews
rage - by Bioz Stars, 2026-03
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p akt  (Proteintech)
96
Proteintech p akt
Fig. 7. CSP supplementation reduced D-gal-induced overexpression of <t>RAGE,</t> BACE-1, Aβ-42 <t>and</t> <t>PS1.</t> (A) Western blot analysis of RAGE, BACE-1, Aβ-42 and PS1; (B) The relative protein expressions of RAGE, BACE-1, Aβ-42 and PS1; Data are expressed as the mean ± SEM (n = 3). Differences were denoted as follows: * P < 0.05, ** P < 0.01, *** P < 0.001 compared with NC; # P < 0.05, ## p < 0.01, ### p < 0.001 compared with D-gal; & P < 0.05, && p < 0.01, &&& p < 0.001 compared with CSPL.
P Akt, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/p akt/product/Proteintech
Average 96 stars, based on 1 article reviews
p akt - by Bioz Stars, 2026-03
96/100 stars
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pkm2  (Proteintech)
96
Proteintech pkm2
Fig. 7. Astragalin (AST) eliminated lipopolysaccharide (LPS)-induced inflammatory response by inhibiting glycolysis enhancement mediated by the hypoxia- inducible factor-1α/pyruvate kinase M2 <t>(HIF-1α/PKM2)</t> signaling pathway. (A) LPS changed the concentrations of adenosine triphosphate (ATP) and lactic acid (LA) in liver and serum, and AST reversed these changes. Student’s t-test was performed, n = 4, * P < 0.05, ** P < 0.01, *** P < 0.001. (B) The activities of hexokinase (HK), pyruvate kinase (PK), and lactate dehydrogenase (LDH) in liver and serum were enhanced by LPS, whereas AST inhibited the enhancement. Student’s t-test was performed, n = 4, * P < 0.05, ** P < 0.01, *** P < 0.001. (C) Glycolysis-related genes, including HK2, LDHA, glucose-6-phosphate dehydrogenase X-linked (G6pdx), and glucose transporter 1 (Glut-1), were upregulated by LPS but AST prevented the upregulation. Similar results also showed in the expression of HIF-1α and PKM2 genes. Student’s t-test was performed, n = 4, * P < 0.05, ** P < 0.01, *** P < 0.001. (D-E) Expressions of HIF-1α and PKM2 proteins were increased by LPS treatment, while AST decreased their expression. Student’s t-test was performed, n = 3, * P < 0.05, ** P < 0.01.
Pkm2, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pkm2/product/Proteintech
Average 96 stars, based on 1 article reviews
pkm2 - by Bioz Stars, 2026-03
96/100 stars
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tbk1  (Proteintech)
96
Proteintech tbk1
Fig. 7. Astragalin (AST) eliminated lipopolysaccharide (LPS)-induced inflammatory response by inhibiting glycolysis enhancement mediated by the hypoxia- inducible factor-1α/pyruvate kinase M2 <t>(HIF-1α/PKM2)</t> signaling pathway. (A) LPS changed the concentrations of adenosine triphosphate (ATP) and lactic acid (LA) in liver and serum, and AST reversed these changes. Student’s t-test was performed, n = 4, * P < 0.05, ** P < 0.01, *** P < 0.001. (B) The activities of hexokinase (HK), pyruvate kinase (PK), and lactate dehydrogenase (LDH) in liver and serum were enhanced by LPS, whereas AST inhibited the enhancement. Student’s t-test was performed, n = 4, * P < 0.05, ** P < 0.01, *** P < 0.001. (C) Glycolysis-related genes, including HK2, LDHA, glucose-6-phosphate dehydrogenase X-linked (G6pdx), and glucose transporter 1 (Glut-1), were upregulated by LPS but AST prevented the upregulation. Similar results also showed in the expression of HIF-1α and PKM2 genes. Student’s t-test was performed, n = 4, * P < 0.05, ** P < 0.01, *** P < 0.001. (D-E) Expressions of HIF-1α and PKM2 proteins were increased by LPS treatment, while AST decreased their expression. Student’s t-test was performed, n = 3, * P < 0.05, ** P < 0.01.
Tbk1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tbk1/product/Proteintech
Average 96 stars, based on 1 article reviews
tbk1 - by Bioz Stars, 2026-03
96/100 stars
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t4 polynucleotide kinase  (TaKaRa)
96
TaKaRa t4 polynucleotide kinase
Fig. 7. Astragalin (AST) eliminated lipopolysaccharide (LPS)-induced inflammatory response by inhibiting glycolysis enhancement mediated by the hypoxia- inducible factor-1α/pyruvate kinase M2 <t>(HIF-1α/PKM2)</t> signaling pathway. (A) LPS changed the concentrations of adenosine triphosphate (ATP) and lactic acid (LA) in liver and serum, and AST reversed these changes. Student’s t-test was performed, n = 4, * P < 0.05, ** P < 0.01, *** P < 0.001. (B) The activities of hexokinase (HK), pyruvate kinase (PK), and lactate dehydrogenase (LDH) in liver and serum were enhanced by LPS, whereas AST inhibited the enhancement. Student’s t-test was performed, n = 4, * P < 0.05, ** P < 0.01, *** P < 0.001. (C) Glycolysis-related genes, including HK2, LDHA, glucose-6-phosphate dehydrogenase X-linked (G6pdx), and glucose transporter 1 (Glut-1), were upregulated by LPS but AST prevented the upregulation. Similar results also showed in the expression of HIF-1α and PKM2 genes. Student’s t-test was performed, n = 4, * P < 0.05, ** P < 0.01, *** P < 0.001. (D-E) Expressions of HIF-1α and PKM2 proteins were increased by LPS treatment, while AST decreased their expression. Student’s t-test was performed, n = 3, * P < 0.05, ** P < 0.01.
T4 Polynucleotide Kinase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/t4 polynucleotide kinase/product/TaKaRa
Average 96 stars, based on 1 article reviews
t4 polynucleotide kinase - by Bioz Stars, 2026-03
96/100 stars
  Buy from Supplier

Image Search Results


Fig. 4 Effect of hawthorn leather treatment on expression levels of inflammatory cytokines and NF-κB signaling pathway in the liver tissue of mice. mRNA expression level of (A) IL-1β, (B) Nos2, (C) Cox2, (D) TLR4, (E) MyD88, and (F) NF-κB; (G) Images of western blotting; Protein expression ratio of (H) MyD88 to GAPDH and (I) NF-κB to GAPDH. Data are expressed as mean ± SD (8 mice/group). Values of each group with different letters over the bars are significantly different (P < 0.05) by analysis of one-way ANOVA followed by Tukey’s multiple range test.

Journal: Food Science and Human Wellness

Article Title: Sucrose-free hawthorn leathers formulated with fructooligosaccharides and xylooligosaccharides ameliorate high-fat diet induced inflammation, glucose and lipid metabolism in liver of mice

doi: 10.1016/j.fshw.2022.03.033

Figure Lengend Snippet: Fig. 4 Effect of hawthorn leather treatment on expression levels of inflammatory cytokines and NF-κB signaling pathway in the liver tissue of mice. mRNA expression level of (A) IL-1β, (B) Nos2, (C) Cox2, (D) TLR4, (E) MyD88, and (F) NF-κB; (G) Images of western blotting; Protein expression ratio of (H) MyD88 to GAPDH and (I) NF-κB to GAPDH. Data are expressed as mean ± SD (8 mice/group). Values of each group with different letters over the bars are significantly different (P < 0.05) by analysis of one-way ANOVA followed by Tukey’s multiple range test.

Article Snippet: Then, the membrane was incubated with specific primary antibody (1 : 1 000) against MyD88 (CST, cat. number 4283S), NF-κB (p65) (CST, cat. number 8242S), PI3K (p110β) (Proteintech, cat. number 20584-1-AP), AKT (Proteintech, cat. number 10176-2-AP), Thr308 phosphorylated AKT (p-AKT) (CST, cat. number 13038T), mTOR (Proteintech, cat. number 20657-1-AP), Ser2448 phosphorylated mTOR (p-mTOR) (CST, cat. number 5536T), PPAR-γ (CST, cat. number 2435T), FASN (CST, cat. number 3180T), and HSL (CST, cat. number 18381T) overnight at 4 °C, respectively.

Techniques: Expressing, Western Blot

Fig. 7. CSP supplementation reduced D-gal-induced overexpression of RAGE, BACE-1, Aβ-42 and PS1. (A) Western blot analysis of RAGE, BACE-1, Aβ-42 and PS1; (B) The relative protein expressions of RAGE, BACE-1, Aβ-42 and PS1; Data are expressed as the mean ± SEM (n = 3). Differences were denoted as follows: * P < 0.05, ** P < 0.01, *** P < 0.001 compared with NC; # P < 0.05, ## p < 0.01, ### p < 0.001 compared with D-gal; & P < 0.05, && p < 0.01, &&& p < 0.001 compared with CSPL.

Journal: Journal of Functional Foods

Article Title: Protective effects of selenium-enriched peptides from Cardamine violifolia on d-galactose-induced brain aging by alleviating oxidative stress, neuroinflammation, and neuron apoptosis

doi: 10.1016/j.jff.2020.104277

Figure Lengend Snippet: Fig. 7. CSP supplementation reduced D-gal-induced overexpression of RAGE, BACE-1, Aβ-42 and PS1. (A) Western blot analysis of RAGE, BACE-1, Aβ-42 and PS1; (B) The relative protein expressions of RAGE, BACE-1, Aβ-42 and PS1; Data are expressed as the mean ± SEM (n = 3). Differences were denoted as follows: * P < 0.05, ** P < 0.01, *** P < 0.001 compared with NC; # P < 0.05, ## p < 0.01, ### p < 0.001 compared with D-gal; & P < 0.05, && p < 0.01, &&& p < 0.001 compared with CSPL.

Article Snippet: Primary antibodies against NFkβ-p65, RAGE, BACE1, PS1, BAX, BCL2, Caspase-3, HO1, NQO1, and β-actin were purchased from Proteintech (Rosemont, IL, USA).

Techniques: Over Expression, Western Blot

Fig. 7. Astragalin (AST) eliminated lipopolysaccharide (LPS)-induced inflammatory response by inhibiting glycolysis enhancement mediated by the hypoxia- inducible factor-1α/pyruvate kinase M2 (HIF-1α/PKM2) signaling pathway. (A) LPS changed the concentrations of adenosine triphosphate (ATP) and lactic acid (LA) in liver and serum, and AST reversed these changes. Student’s t-test was performed, n = 4, * P < 0.05, ** P < 0.01, *** P < 0.001. (B) The activities of hexokinase (HK), pyruvate kinase (PK), and lactate dehydrogenase (LDH) in liver and serum were enhanced by LPS, whereas AST inhibited the enhancement. Student’s t-test was performed, n = 4, * P < 0.05, ** P < 0.01, *** P < 0.001. (C) Glycolysis-related genes, including HK2, LDHA, glucose-6-phosphate dehydrogenase X-linked (G6pdx), and glucose transporter 1 (Glut-1), were upregulated by LPS but AST prevented the upregulation. Similar results also showed in the expression of HIF-1α and PKM2 genes. Student’s t-test was performed, n = 4, * P < 0.05, ** P < 0.01, *** P < 0.001. (D-E) Expressions of HIF-1α and PKM2 proteins were increased by LPS treatment, while AST decreased their expression. Student’s t-test was performed, n = 3, * P < 0.05, ** P < 0.01.

Journal: Journal of Functional Foods

Article Title: Astragalin protects against lipopolysaccharide-triggered acute liver injury through suppression of necroptosis and inflammation and improvement of energy metabolism

doi: 10.1016/j.jff.2024.106298

Figure Lengend Snippet: Fig. 7. Astragalin (AST) eliminated lipopolysaccharide (LPS)-induced inflammatory response by inhibiting glycolysis enhancement mediated by the hypoxia- inducible factor-1α/pyruvate kinase M2 (HIF-1α/PKM2) signaling pathway. (A) LPS changed the concentrations of adenosine triphosphate (ATP) and lactic acid (LA) in liver and serum, and AST reversed these changes. Student’s t-test was performed, n = 4, * P < 0.05, ** P < 0.01, *** P < 0.001. (B) The activities of hexokinase (HK), pyruvate kinase (PK), and lactate dehydrogenase (LDH) in liver and serum were enhanced by LPS, whereas AST inhibited the enhancement. Student’s t-test was performed, n = 4, * P < 0.05, ** P < 0.01, *** P < 0.001. (C) Glycolysis-related genes, including HK2, LDHA, glucose-6-phosphate dehydrogenase X-linked (G6pdx), and glucose transporter 1 (Glut-1), were upregulated by LPS but AST prevented the upregulation. Similar results also showed in the expression of HIF-1α and PKM2 genes. Student’s t-test was performed, n = 4, * P < 0.05, ** P < 0.01, *** P < 0.001. (D-E) Expressions of HIF-1α and PKM2 proteins were increased by LPS treatment, while AST decreased their expression. Student’s t-test was performed, n = 3, * P < 0.05, ** P < 0.01.

Article Snippet: Adenosine triphosphate (ATP) content detection kit (#BC0305), lactic acid (LA) content assay kit (#BC2235), lactate dehydrogenase (LDH) activity assay kit (#BC0685), hexokinase (HK) activity assay kit (#BC0745), pyruvate kinase (PK) activity assay kit (#BC0545) were obtained from Beijing Solarbio Science & Technology Co., Ltd. Trizol (total RNA extraction reagent, #R0016), RIPA lysis buffer (strong, #P0013B), and BCA protein assay kit (#P0011) were provided by Beyotime Biotech Inc. Plus All-in-one 1st strand cDNA synthesis supermix (gDNA Purge, #E047-01B) and SYBR qPCR supermix plus (#E096-01B) were obtained from Novoprotein Scientific Inc. We got TNF-α polyclonal antibody (#17590–1-AP), TNFR1associated death domain protein (TRADD) polyclonal antibody (#15468–1-AP), HIF-1α polyclonal antibody (#20960–1-AP), PKM2, muscle2-specific monoclonal antibody (#60268–1-Ig), β-actin recombinant antibody (#81115–1-RR), and TANK-binding kinase 1 (TBK1) polyclonal antibody (#28397–1-AP) from Proteintech Group, Inc. Phospho-TBK1 (P-TBK1, Ser172) polyclonal antibody (#BD-PP1527) was provided by Biodragon Co. Ltd. Anti-phospho-RIP family of serinethreonine kinases (Ser166, P-RIPK1) polyclonal antibodies (#31122S), RIPK1 (D94C12) monoclonal antibody (#3493S), RIPK3 (D8J3L) monoclonal antibody (#15828S), and anti-phospho-RIPK3 (Thr231/ Ser232, E7S1R, #91702) were obtained from Cell Signaling Technology, Inc. Anti-phospho-MLKL (Ser345, P-MLKL) monoclonal antibody (#MABC1158) was provided by Merck Millipore Corporation, while MLKL polyclonal antibody (#PA5-71886) was provided by Thermo Fisher Scientific Inc. Bio-Rad Laboratories Co. Ltd provided CD68 monoclonal antibody (#MCA1957).

Techniques: Expressing

Fig. 8. The involved molecular mechanisms of astragalin (AST) in the treatment of lipopolysaccharide (LPS)-induced acute liver injury (ALI). LPS promotes the M1 type macrophage activation, causing inflammatory responses and the release of inflammatory cytokines, including tumor necrosis factor (TNF)-α. By binding to its receptor, TNF-α activated the receptor-interacting protein kinase 1 (RIPK1)/RIPK3/ mixed lineage kinase domain-like protein (MLKL) signal axis and mediated necroptosis. Meanwhile, LPS triggered the hypoxia-inducible factor-1α/pyruvate kinase M2 (HIF-1α/PKM2) signaling pathway, which mediates glycolysis-enhanced inflammatory response and oxidative stress. However, AST inhibited the transition of macrophages into M1 type, reduced the expression of pro-inflammatory cy tokines, which further decreased the activation of related signaling pathways, thereby preventing the liver from acute injury. This figure is created by Figdraw.

Journal: Journal of Functional Foods

Article Title: Astragalin protects against lipopolysaccharide-triggered acute liver injury through suppression of necroptosis and inflammation and improvement of energy metabolism

doi: 10.1016/j.jff.2024.106298

Figure Lengend Snippet: Fig. 8. The involved molecular mechanisms of astragalin (AST) in the treatment of lipopolysaccharide (LPS)-induced acute liver injury (ALI). LPS promotes the M1 type macrophage activation, causing inflammatory responses and the release of inflammatory cytokines, including tumor necrosis factor (TNF)-α. By binding to its receptor, TNF-α activated the receptor-interacting protein kinase 1 (RIPK1)/RIPK3/ mixed lineage kinase domain-like protein (MLKL) signal axis and mediated necroptosis. Meanwhile, LPS triggered the hypoxia-inducible factor-1α/pyruvate kinase M2 (HIF-1α/PKM2) signaling pathway, which mediates glycolysis-enhanced inflammatory response and oxidative stress. However, AST inhibited the transition of macrophages into M1 type, reduced the expression of pro-inflammatory cy tokines, which further decreased the activation of related signaling pathways, thereby preventing the liver from acute injury. This figure is created by Figdraw.

Article Snippet: Adenosine triphosphate (ATP) content detection kit (#BC0305), lactic acid (LA) content assay kit (#BC2235), lactate dehydrogenase (LDH) activity assay kit (#BC0685), hexokinase (HK) activity assay kit (#BC0745), pyruvate kinase (PK) activity assay kit (#BC0545) were obtained from Beijing Solarbio Science & Technology Co., Ltd. Trizol (total RNA extraction reagent, #R0016), RIPA lysis buffer (strong, #P0013B), and BCA protein assay kit (#P0011) were provided by Beyotime Biotech Inc. Plus All-in-one 1st strand cDNA synthesis supermix (gDNA Purge, #E047-01B) and SYBR qPCR supermix plus (#E096-01B) were obtained from Novoprotein Scientific Inc. We got TNF-α polyclonal antibody (#17590–1-AP), TNFR1associated death domain protein (TRADD) polyclonal antibody (#15468–1-AP), HIF-1α polyclonal antibody (#20960–1-AP), PKM2, muscle2-specific monoclonal antibody (#60268–1-Ig), β-actin recombinant antibody (#81115–1-RR), and TANK-binding kinase 1 (TBK1) polyclonal antibody (#28397–1-AP) from Proteintech Group, Inc. Phospho-TBK1 (P-TBK1, Ser172) polyclonal antibody (#BD-PP1527) was provided by Biodragon Co. Ltd. Anti-phospho-RIP family of serinethreonine kinases (Ser166, P-RIPK1) polyclonal antibodies (#31122S), RIPK1 (D94C12) monoclonal antibody (#3493S), RIPK3 (D8J3L) monoclonal antibody (#15828S), and anti-phospho-RIPK3 (Thr231/ Ser232, E7S1R, #91702) were obtained from Cell Signaling Technology, Inc. Anti-phospho-MLKL (Ser345, P-MLKL) monoclonal antibody (#MABC1158) was provided by Merck Millipore Corporation, while MLKL polyclonal antibody (#PA5-71886) was provided by Thermo Fisher Scientific Inc. Bio-Rad Laboratories Co. Ltd provided CD68 monoclonal antibody (#MCA1957).

Techniques: Activation Assay, Binding Assay, Expressing, Protein-Protein interactions