Journal: bioRxiv

Article Title: Genetic regulators of neuronal survival across metabolic environments

doi: 10.64898/2025.12.19.695350

Figure Lengend Snippet: A) Volcano plot of genes up- or down-regulated in KIAA1429 knockdown vs NTG control neurons. Glycolytic genes are more strongly affected than mitochondrial complex I assembly genes. Data compiled from n = 2 samples per condition. B) Plot of transcripts’ abundance in KIAA1429 KD cells and NTG control cells after 4-hour exposure to transcription inhibitor actinomycin relative to before exposure. Knockdown in KIAA1429 was associated with decreased abundance of SOX2 and NGF-related transcription targets (dark blue) after treatment with actinomycin. The expression of these transcripts was not significantly decreased in neuronal cells expressing a non-targeting guide, also subjected to treatment with actinomycin, indicating that KIAA1429 activity has a stabilizing effect on these transcripts. In contrast, glycolytic transcripts (red) significantly increased expression in neuronal cells with KIAA1429 knockdown and actinomycin treatment, indicating that stability of these transcripts was not impacted by KIAA1429 expression. Data compiled from n = 2 samples per condition. All “hit” genes have adjusted p-values < 0.05.

Article Snippet: Gene expression was measured by real-time PCR on QuantStudio 5 using FAM-MGB Taqman Gene Expression Assays (ThermoFisher, assay ID: Hs00198702_m1 GLO1 , Hs00936421_m1 KIAA1429 , Hs01556304_m1 TDRD3 , Hs00902194_m1 MAPT , Hs00175976_m1 HK1 , Hs00976715_m1 GPI , Hs01075411_m1 PFKM , Hs01378790_g1 LDHA , Hs00761782_s1 PKM , Hs01652468_g1 PGAM1 , Hs00361415_m1 ENO1 ) together with VIC-MGB human ACTB (ThermoFisher, #4326315E).

Techniques: Knockdown, Control, Expressing, Activity Assay

Journal: bioRxiv

Article Title: Genetic regulators of neuronal survival across metabolic environments

doi: 10.64898/2025.12.19.695350

Figure Lengend Snippet: A) Heatmap of the cluster of genes enriched for dendrite membrane components from includes MAPT , KIAA1429, and TDRD3 . Knockdown of these genes results in a common phenotype of poor survival in the high-oxygen glycolytic state (20G), while retaining survival in the high oxygen respiratory state (20R). B) Proposed mechanism by which knocking down genes that support various steps in late glycolysis results in diversion of glucose to upstream glycolytic branch metabolites, including nucleotides. C) Bubble plot of enriched metabolic pathways among metabolites with increased or decreased 13C-glucose derived labeling in neurons with genetic knockdowns. Bubble radius corresponds to pathway impact, which is a measure of the centrality of enriched metabolites. In the high-oxygen glycolytic state, knockdown of these three functionally similar genes in neuronal cells was associated with increased 13C-glucose-derived labeling across purine metabolites, compared to neuronal cells expressing a non-targeting sgRNA. In contrast, knockdown of GLO1 , a gene less related gene by hierarchical clustering, did not increase 13C-glucose-derived labeling in purine metabolites. D) UDP, AMP, and ADP metabolites specifically are significantly increased 13C-glucose-derived labeling associated with knockdown in MAPT , KIAA1429 , and TDRD3 but not GLO1 in the high-oxygen glycolytic state. E) TCA cycle metabolites are among the metabolites that have less 13C-glucose-derived labeling in the high-oxygen glycolytic state.

Article Snippet: Gene expression was measured by real-time PCR on QuantStudio 5 using FAM-MGB Taqman Gene Expression Assays (ThermoFisher, assay ID: Hs00198702_m1 GLO1 , Hs00936421_m1 KIAA1429 , Hs01556304_m1 TDRD3 , Hs00902194_m1 MAPT , Hs00175976_m1 HK1 , Hs00976715_m1 GPI , Hs01075411_m1 PFKM , Hs01378790_g1 LDHA , Hs00761782_s1 PKM , Hs01652468_g1 PGAM1 , Hs00361415_m1 ENO1 ) together with VIC-MGB human ACTB (ThermoFisher, #4326315E).

Techniques: Membrane, Knockdown, Derivative Assay, Labeling, Expressing

Journal: BMC Cancer

Article Title: N6-methyladenosine RNA modification (m6A) is of prognostic value in HPV-dependent vulvar squamous cell carcinoma

doi: 10.1186/s12885-022-10010-x

Figure Lengend Snippet: Summary of the analyzed m6A proteins as indicated and their correlation with overall survival (indicated as %alive) for the entire study cohort, HPV-independent, and HPV-dependent VSCC. The HPV-status was not available for 24 patients. Samples were grouped according to high and low expression based on the staining intensities. p -values for the group comparisons are based on log-rank tests (significance threshold p < 0.5). q -values are based on multiple hypotheses testing using the method of Benjamini and Hochberg with a significance threshold of q < 0.1

Article Snippet: Immunostaining of METTL3, METTL4, METTL14, WTAP, KIAA1429, FTO, ALKBH5, HNRNPA2B1, HNRNPC, YTHDC1, YTHDF1,YTHDF2, and YTHDF3 was performed on the TMAs using an automated staining system (BenchMark ULTRA; Ventana Medical Systems) which performed deparaffinization, pretreatment with cell conditioning buffer (CC1 buffer, pH8), and incubation with primary antibodies (FTO (1:50; Atlas Antibodies #HPA041086), ALKBH5 (1:200; Novus #NBP1-82,188), METTL3 (1:1000; Biorbyt #orb374082), METTL4 (1:40; Atlas Antibodies #HPA040061), METTL14 (1:100; Atlas Antibodies #HPA038002), WTAP (1:100; Atlas Antibodies #HPA010550), KIAA1429 (1:25; Atlas Antibodies #HPA031530), HNRNPC (1:25; Atlas Antibodies #HPA051075), HNRNPA2B1 (1:100; Atlas Antibodies #HPA001666), YTHDC1 (1:25; Atlas Antibodies #HPA036462), YTHDF1 (1:10; Biorbyt #orb179018), YTHDF2 (1:200; Biorbyt #orb39199), YTHDF3 (1:200; Biorbyt #orb374095) at 4 °C overnight.

Techniques: Expressing, Staining

Journal: Cell Cycle

Article Title: KIAA1429 promotes osteosarcoma progression by promoting stem cell properties and is regulated by miR-143-3p

doi: 10.1080/15384101.2020.1749465

Figure Lengend Snippet: Correlation of clinico-pathological features with KIAA1429 expression in osteosarcoma cohort.

Article Snippet: The coding sequences of human KIAA1429 and negative control were amplified and cloned into pcDNA3.1 (+) to generate KIAA1429 overexpression and according negative control (GenePharma, Shanghai, China). miR-143-3p mimics, inhibitors as well as respective negative controls (NC) were commercially purchased from Gene Pharma (Shanghai, China).

Techniques: Expressing

Journal: Cell Cycle

Article Title: KIAA1429 promotes osteosarcoma progression by promoting stem cell properties and is regulated by miR-143-3p

doi: 10.1080/15384101.2020.1749465

Figure Lengend Snippet: Correlation of clinic-pathological features with KIAA1429 expression in OS cohort.

Article Snippet: The coding sequences of human KIAA1429 and negative control were amplified and cloned into pcDNA3.1 (+) to generate KIAA1429 overexpression and according negative control (GenePharma, Shanghai, China). miR-143-3p mimics, inhibitors as well as respective negative controls (NC) were commercially purchased from Gene Pharma (Shanghai, China).

Techniques: Expressing