Journal: Nanomaterials

Article Title: Effects of Two Kinds of Iron Nanoparticles as Reactive Oxygen Species Inducer and Scavenger on the Transcriptomic Profiles of Two Human Leukemia Cells with Different Stemness

doi: 10.3390/nano10101951

Figure Lengend Snippet: The characterization of two kinds of iron nanoparticles and their effects on KG1a and HL60: ( A , B ) hydrated particle size and zeta potential of Prussian blue nanoparticles (PBNPs) ( A ) and Fe 3 O 4 nanoparticles (FeNPs) ( B ), ( C ) cell viability of the PBNP- and FeNP-treated HL60 and KG1a cells, ( D ) cellular iron contents of the PBNP- and FeNP-treated HL60 and KG1a cells, and ( E ) QPCR detection of CD34 and CD38 expression in two cells. HF, FeNP-treated HL60; HP, PBNP-treated HL60; KF, FeNP-treated KG1a; KP, PBNP-treated KG1a. ns, no significance; *, p < 0.05; **, p < 0.01; ***, p < 0.001. ( F ) ROS levels of the PBNP- and FeNP-treated HL60 and KG1a cells.

Article Snippet: The human acute myelogenous leukemia (AML) cell KG1a and the human acute promyelocytic leukemia (APL) cell HL60 were obtained by the China Center for Type Culture Collection (Shanghai, China).

Techniques: Zeta Potential Analyzer, Expressing

Journal: Nanomaterials

Article Title: Effects of Two Kinds of Iron Nanoparticles as Reactive Oxygen Species Inducer and Scavenger on the Transcriptomic Profiles of Two Human Leukemia Cells with Different Stemness

doi: 10.3390/nano10101951

Figure Lengend Snippet: Comparisons of differentially expressed genes (DEGs) in two Leukemia cells treated with two kinds of iron nanoparticles: ( A ) number of upregulated and downregulated DEGs (fold change > 1.0) in the PBNP- and FeNP-treated HL60 and KG1a cells and their relationship, ( B ) common DEGs (fold change > 1.5) in a cell treated by two kinds of nanoparticles, ( C ) common DEGs (fold change > 1.5) in two cells treated by a nanoparticle, ( D ) common DEGs (fold change > 1.0) in two cells treated by two kinds of nanoparticles, and ( E ) schematic of cellular functions of common DEGs (fold change > 1.0) in two cells treated by two kinds of nanoparticles. The detailed information of all DEGs is shown in . HF, FeNP-treated HL60 cells; HP, PBNP-treated HL60 cells; KF, FeNP-treated KG1a cells; and KP, PBNP-treated KG1a cells.

Article Snippet: The human acute myelogenous leukemia (AML) cell KG1a and the human acute promyelocytic leukemia (APL) cell HL60 were obtained by the China Center for Type Culture Collection (Shanghai, China).

Techniques:

Journal: Nanomaterials

Article Title: Effects of Two Kinds of Iron Nanoparticles as Reactive Oxygen Species Inducer and Scavenger on the Transcriptomic Profiles of Two Human Leukemia Cells with Different Stemness

doi: 10.3390/nano10101951

Figure Lengend Snippet: Top 20 GO terms of the KG1a cells treated with two kinds of iron nanoparticles: ( A , B ) heatmap ( A ) and enrichment network ( B ) colored by the same cluster of GO terms in the FeNP-treated KG1a cells, and ( C , D ) heatmap ( C ) and enrichment network ( D ) colored by the same cluster of GO terms in the PBNP-treated KG1a cells. The colored labels followed the order of top 20 GO terms. ( E ) Venn analysis of top 20 GO terms in the KG1a cells treated with PBNPs and FeNPs. The detailed information of all GO terms is shown in .

Article Snippet: The human acute myelogenous leukemia (AML) cell KG1a and the human acute promyelocytic leukemia (APL) cell HL60 were obtained by the China Center for Type Culture Collection (Shanghai, China).

Techniques:

Journal: Nanomaterials

Article Title: Effects of Two Kinds of Iron Nanoparticles as Reactive Oxygen Species Inducer and Scavenger on the Transcriptomic Profiles of Two Human Leukemia Cells with Different Stemness

doi: 10.3390/nano10101951

Figure Lengend Snippet: GO terms of lipid metabolisms in KG1a and HL60 exposed to FeNPs or PBNPs (Top 20, p < 0.01).

Article Snippet: The human acute myelogenous leukemia (AML) cell KG1a and the human acute promyelocytic leukemia (APL) cell HL60 were obtained by the China Center for Type Culture Collection (Shanghai, China).

Techniques: Membrane, Activity Assay

Journal: Nanomaterials

Article Title: Effects of Two Kinds of Iron Nanoparticles as Reactive Oxygen Species Inducer and Scavenger on the Transcriptomic Profiles of Two Human Leukemia Cells with Different Stemness

doi: 10.3390/nano10101951

Figure Lengend Snippet: GO terms of metal ion metabolisms in KG1a and HL60 exposed to FeNPs or PBNPs (Top 20, p < 0.01).

Article Snippet: The human acute myelogenous leukemia (AML) cell KG1a and the human acute promyelocytic leukemia (APL) cell HL60 were obtained by the China Center for Type Culture Collection (Shanghai, China).

Techniques: Activity Assay, Clinical Proteomics, Membrane

Journal: Nanomaterials

Article Title: Effects of Two Kinds of Iron Nanoparticles as Reactive Oxygen Species Inducer and Scavenger on the Transcriptomic Profiles of Two Human Leukemia Cells with Different Stemness

doi: 10.3390/nano10101951

Figure Lengend Snippet: Comparative Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of the DEGs ( p < 0.05): ( A ) comparative top 10 enriched pathways of HL60 and KG1a treated with FeNPs and ( B ) comparative top 10 enriched pathways of HL60 and KG1a treated with PBNPs. The pathways in black and blue were HL60 and KG1a cells, respectively. ( C ) Four-way Venn analysis of all KEGG pathways in four groups. The detailed information of all KEGG pathways is shown in . HF, FeNP-treated HL60 cells; HP, PBNP-treated HL60 cells; KF, FeNP-treated KG1a cells; and KP, PBNP-treated KG1a cells.

Article Snippet: The human acute myelogenous leukemia (AML) cell KG1a and the human acute promyelocytic leukemia (APL) cell HL60 were obtained by the China Center for Type Culture Collection (Shanghai, China).

Techniques:

Journal: Nanomaterials

Article Title: Effects of Two Kinds of Iron Nanoparticles as Reactive Oxygen Species Inducer and Scavenger on the Transcriptomic Profiles of Two Human Leukemia Cells with Different Stemness

doi: 10.3390/nano10101951

Figure Lengend Snippet: Validation of RNA-Seq DEGs using RT-qPCR: ( A , B ) the expression levels of 10 selected DEGs in the iron nanoparticle-treated HL60 ( A ) and KG1a ( B ) cells. All values are mean ± SD with n = 3. ns, no significance; *, p < 0.05; **, p < 0.01, ***, p < 0.001, ****, and p < 0.0001. ( C , D ) The comparison of fold change detected by RT-qPCR and RNA-Seq in the iron nanoparticle-treated HL60 and ( C , D ) KG1a cells.

Article Snippet: The human acute myelogenous leukemia (AML) cell KG1a and the human acute promyelocytic leukemia (APL) cell HL60 were obtained by the China Center for Type Culture Collection (Shanghai, China).

Techniques: Biomarker Discovery, RNA Sequencing, Quantitative RT-PCR, Expressing, Comparison

Journal: International Journal of Molecular Sciences

Article Title: Involvement of GPx-3 in the Reciprocal Control of Redox Metabolism in the Leukemic Niche

doi: 10.3390/ijms21228584

Figure Lengend Snippet: Mesenchymal stromal cells (MSCs) decreased leukemic cell proliferation. Experiments were performed with KG1a leukemic cells cultured alone (blue), with MSC-CM (green), or in coculture with MSCs (red). ( A ) Experimental design of mono- and coculture of MSCs and leukemic KG1a cells. Leukemic cells were cultured in medium alone, with MSC-CM or over MSCs; MSCs were cultured alone or with KG1a leukemic cells. ( B ) Leukemic cell growth was evaluated after 72 h of mono-, MSC-CM, or coculture (nonadherent cells represent cells that do not adhere to MSCs after 72 h of coculture, n = 15). ( C ) Cell cycle was analyzed by a flow cytometry multilabeling protocol using anti-Ki67-AF488, anti-phosphoS10-H3-AF488 and 7AAD ( n = 5). Variations in MSC-CM and cocultured vs. monocultured KG1a in cell cycle phases after 72h are shown on the left and a representative experiment is presented on the right. * p < 0.05.

Article Snippet: The human acute myeloid leukemia cell-line KG1a (FAB M0/M1, CD34 + ) was purchased from the European Collection of Authenticated Cell Cultures (ECACC, Wiltshire, UK) and cultured in Minimum Essential Medium Alpha (αMEM, Gibco BRL, ThermoFisher Scientific, Waltham, MA, USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS, HyClone), 2 mM L-glutamine (Gibco BRL), 100 IU/mL penicillin, and 100 μg/mL streptomycin (Gibco BRL).

Techniques: Cell Culture, Flow Cytometry

Journal: International Journal of Molecular Sciences

Article Title: Involvement of GPx-3 in the Reciprocal Control of Redox Metabolism in the Leukemic Niche

doi: 10.3390/ijms21228584

Figure Lengend Snippet: Contact with MSCs increases SP proportion in leukemic cells. SP was assessed by Hoechst efflux measurement in flow cytometry. Cytograms in ( A ) illustrate a representative acquisition of Hoechst staining of KG1a leukemic cells after a 72 h culture alone (left panel), in MSC-CM (middle panel), or with MSC-contact (right panel). Quantitative results are shown as SP percentages ( B ) absolute numbers ( C ) in the three culture conditions ( n = 4). * p < 0.05.

Article Snippet: The human acute myeloid leukemia cell-line KG1a (FAB M0/M1, CD34 + ) was purchased from the European Collection of Authenticated Cell Cultures (ECACC, Wiltshire, UK) and cultured in Minimum Essential Medium Alpha (αMEM, Gibco BRL, ThermoFisher Scientific, Waltham, MA, USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS, HyClone), 2 mM L-glutamine (Gibco BRL), 100 IU/mL penicillin, and 100 μg/mL streptomycin (Gibco BRL).

Techniques: Flow Cytometry, Staining

Journal: International Journal of Molecular Sciences

Article Title: Involvement of GPx-3 in the Reciprocal Control of Redox Metabolism in the Leukemic Niche

doi: 10.3390/ijms21228584

Figure Lengend Snippet: MSCs decrease the energy metabolism of leukemic cells. Energy metabolism was assessed through the evaluation of mitochondrial respiration (OCR) and glycolysis (ECAR) and with Seahorse XFe96 ( n = 7). ( A ) Analysis of energy metabolism in KG1a cells. ( B ) Analysis of energy metabolism in MSCs. * p < 0.05.

Article Snippet: The human acute myeloid leukemia cell-line KG1a (FAB M0/M1, CD34 + ) was purchased from the European Collection of Authenticated Cell Cultures (ECACC, Wiltshire, UK) and cultured in Minimum Essential Medium Alpha (αMEM, Gibco BRL, ThermoFisher Scientific, Waltham, MA, USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS, HyClone), 2 mM L-glutamine (Gibco BRL), 100 IU/mL penicillin, and 100 μg/mL streptomycin (Gibco BRL).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Involvement of GPx-3 in the Reciprocal Control of Redox Metabolism in the Leukemic Niche

doi: 10.3390/ijms21228584

Figure Lengend Snippet: Interaction between leukemic cells and MSCs induces opposite oxidative metabolism and Nrf2 pathway modifications in both cell types. ( A ) Intracellular ROS level was analyzed by flow cytometry after CM-H 2 DCFDA staining in leukemic cells cultured alone (blue), with MSC-CM (green), or with MSCs (red) as well as in MSCs cultured alone (violet) or cocultured with KG1a cells (dark violet) for 72h ( n = 5–9). ( B ) The expression and activation of p38MAPK were analyzed by Western blot in KG1a cultured alone, with MSC-CM or cocultured as well as in MSCs alone or cocultured with leukemic cells ( n = 3); alpha-tubulin was used as loading control. ( C ) Nrf2 subcellular localization was analyzed by Western blot in the cytoplasmic and nuclear fractions of leukemic cells (left) or MSCs (right). RAF and TOPO-1 were used as loading controls and purity indicators of the cytoplasmic and nuclear fractions, respectively. ( D ) Nrf2 target genes expression was evaluated by transcriptomic analysis and is presented as relative expression vs. KG1a cells or MSCs alone ( n = 3). * p < 0.05.

Article Snippet: The human acute myeloid leukemia cell-line KG1a (FAB M0/M1, CD34 + ) was purchased from the European Collection of Authenticated Cell Cultures (ECACC, Wiltshire, UK) and cultured in Minimum Essential Medium Alpha (αMEM, Gibco BRL, ThermoFisher Scientific, Waltham, MA, USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS, HyClone), 2 mM L-glutamine (Gibco BRL), 100 IU/mL penicillin, and 100 μg/mL streptomycin (Gibco BRL).

Techniques: Flow Cytometry, Staining, Cell Culture, Expressing, Activation Assay, Western Blot, Control

Journal: International Journal of Molecular Sciences

Article Title: Involvement of GPx-3 in the Reciprocal Control of Redox Metabolism in the Leukemic Niche

doi: 10.3390/ijms21228584

Figure Lengend Snippet: Leukemic cells and MSCs reciprocally modify their gene expression profile of antioxidant enzymes. ( A ) Gene expression of the major antioxidant enzymes was analyzed by real-time PCR in KG1a cells and is presented, from the highest to the lowest expressed genes in KG1a cells, as the percentage of increased or decreased relative expression (RQ = 2 −ΔΔ C t ) in KG1a cells cultured in MSC-CM (green dots) or cocultured with MSCs (red dots) vs. KG1a cells alone (blue line) (left). Increased GPX3 expression was studied at the protein level by Western blot analysis (right), alpha-tubulin being used as loading control. ( B ) Gene expression of major antioxidant enzymes was analyzed by real-time PCR in MSCs and is presented, from the highest to the lowest expressed gene in MSCs alone, as the percentage of increased or decreased relative expression (RQ = 2 −ΔΔ C t ) in cocultured MSCs (violet dots) vs. MSCs alone (violet line) (left). Decreased GPX3 expression was confirmed at the protein level by Western blot analysis (right), alpha-tubulin being used as loading control. * p < 0.05.

Article Snippet: The human acute myeloid leukemia cell-line KG1a (FAB M0/M1, CD34 + ) was purchased from the European Collection of Authenticated Cell Cultures (ECACC, Wiltshire, UK) and cultured in Minimum Essential Medium Alpha (αMEM, Gibco BRL, ThermoFisher Scientific, Waltham, MA, USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS, HyClone), 2 mM L-glutamine (Gibco BRL), 100 IU/mL penicillin, and 100 μg/mL streptomycin (Gibco BRL).

Techniques: Gene Expression, Real-time Polymerase Chain Reaction, Expressing, Cell Culture, Western Blot, Control

Journal: Toxins

Article Title: Scorpion Peptide Smp24 Exhibits a Potent Antitumor Effect on Human Lung Cancer Cells by Damaging the Membrane and Cytoskeleton In Vivo and In Vitro

doi: 10.3390/toxins14070438

Figure Lengend Snippet: Viability and morphology changes of cancer cells induced by Smp24. ( A ) Viability of A549, H3122, PC-9, H460 and MRC-5 cells treated with Smp24 for 24 h. ( B ) Proliferation of A549 cells treated with the indicated concentrations of Smp24 for 24 h. Panels ( a – d ) are sequentially the cells treated with 0, 1.25, 2.5, and 5 μM of Smp24. ( C ) Cell morphology changes after treatment with Smp24 for 24 h. Panels ( a – f ) are sequentially the cells treated with 0, 1.25, 2.5, 5, 10 and 20 μM of Smp24. Scale bar, 100 μm. Data are normalized to control and presented as mean ± SEM (n = 3).

Article Snippet: The peptide shows stronger cytotoxicity against leukemic tumor cell lines (KG1-a and CCRF-CEM) than the normal cell lines (CD34 + , HRECs and HACAT) [ ], which indicates that Smp24 may be a potential ACP.

Techniques: Control

Journal: Toxins

Article Title: Scorpion Peptide Smp24 Exhibits a Potent Antitumor Effect on Human Lung Cancer Cells by Damaging the Membrane and Cytoskeleton In Vivo and In Vitro

doi: 10.3390/toxins14070438

Figure Lengend Snippet: Necrosis induced by Smp24 in A549 cells. ( A ) LDH release of A549 cells induced by Smp24 (0–20 µM) for 12, 24 and 48 h. ( B ) SEM analysis of morphological structure of A549 cells. Panel ( a ): control A549 cells; panels ( b , c ): A549 cells treated by 2.5 and 5 µM of Smp24. Scale bar, 10 μm. ( C ) Representative flow cytometry analysis of calcein AM changes in A549 cells treated with Smp24 for 24 h. Panels ( a – d ): cells stained with calcein AM; panels ( a’ – d’ ): cells treated by calcein AM + CoCl 2 ; Panels ( a – a’ , b – b’ , c – c’ , d – d’ ): cells treated by 0, 1.25, 2.5 and 5 µM of Smp24, respectively. Results are presented as mean ± SEM (n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001 are considered statistically significant as compared to the control. ( D , E ) Effects of inhibitors on the viability and the LDH release of Smp24-treated A549 cells. A549 cells were pre-incubated with the inhibitors (40 μM Necrostatin-1, 1 μM Cyclosporine A) for 30 min before being further incubated with 5 μM Smp24 for 12 h. ( F ) Fluorescence microscope observation of 5 μM FITC-labeled Smp24 internalized in A549 cells after co-incubation for 24 h. ( a , b ) Control (no treatment) group; ( a’ , b’ ) 24 h treatment group; panels ( c , c’ ) are the merged figure. Yellow arrow: nuclear fragmentation or the apoptotic body. Scale bar, 20 μm.

Article Snippet: The peptide shows stronger cytotoxicity against leukemic tumor cell lines (KG1-a and CCRF-CEM) than the normal cell lines (CD34 + , HRECs and HACAT) [ ], which indicates that Smp24 may be a potential ACP.

Techniques: Control, Flow Cytometry, Staining, Incubation, Fluorescence, Microscopy, Labeling

Journal: Toxins

Article Title: Scorpion Peptide Smp24 Exhibits a Potent Antitumor Effect on Human Lung Cancer Cells by Damaging the Membrane and Cytoskeleton In Vivo and In Vitro

doi: 10.3390/toxins14070438

Figure Lengend Snippet: Effects of Smp24 on the motility and cytoskeleton reorganization of A549 cells. ( A ) Fluorescence staining images of F-actin. Upper panel: cells stained with rhodamine-phalloidin; middle panel: cells stained with DAPI; lower panel: the merging images of cells stained by rhodamine–phalloidin and DAPI. A549 cells were treated with Smp24 (0, 2.5, and 5 μM) for 24 h and successively stained by rhodamine-phalloidin as well as DAPI before fluorescence microscopy observation. White arrow: disordered microfilament bundles. Scale bar, 20 μm. ( B ) Representative pictures of A549 cells in the scratch migration assay at 0 and 24 h following incubation with Smp24 and PBS. Panels ( a – a’ , b – b’ , c – c’ , d – d’ ): the cells treated by 0, 0.3, 0.6 and 1.2 µM of Smp24, respectively. ( C ) Statistical analysis for the scratch migration assay. ( D ) Typical images of A549 cells in the transwell invasion assay following culture with Smp24 and PBS for 24 h. Panels ( a – d ): the cells treated by 0, 0.3, 0.6 and 1.2 µM of Smp24, respectively. ( E ) Statistical analysis of relative mRNA contents of MMP-2, MMP-9, TIMP-1 and TIMP-2. Results are mean ± SEM (n = 3). ** p < 0.01 and *** p < 0.001 are considered statistically significant as compared to the control group without Smp24.

Article Snippet: The peptide shows stronger cytotoxicity against leukemic tumor cell lines (KG1-a and CCRF-CEM) than the normal cell lines (CD34 + , HRECs and HACAT) [ ], which indicates that Smp24 may be a potential ACP.

Techniques: Fluorescence, Staining, Microscopy, Migration, Incubation, Transwell Invasion Assay, Control

Journal: Toxins

Article Title: Scorpion Peptide Smp24 Exhibits a Potent Antitumor Effect on Human Lung Cancer Cells by Damaging the Membrane and Cytoskeleton In Vivo and In Vitro

doi: 10.3390/toxins14070438

Figure Lengend Snippet: In vivo antitumor effects of Smp24. ( A ) Experimental schedule for xenograft mice. ( B ) Images of tumors from sacrificed nude mice. ( C ) Tumor weight. ( D ) Tumor volume. ( E ) Body weight change. ( F ) The weight of heart, liver, spleen, lung and kidney. ( G ) HE staining analysis of tumor tissue. Scale bar, 50 μm. ( H ) Immunohistochemical analysis of cleaved caspase-3 in tumor tissue from lung carcinoma xenografts. Panel ( a ): control group; panel ( b ): Smp24-treated group. Scale bar, 50 μm. Data presented are mean ± SEM (n = 5). ns: no significance, * p < 0.05, ** p < 0.01 and *** p < 0.001 are considered statistically significant as compared to the control group without Smp24 treatment. Yellow arrow: enlarged tumor cells. Blue arrow: shrinking cells. White arrow: positive apoptotic staining (brown areas).

Article Snippet: The peptide shows stronger cytotoxicity against leukemic tumor cell lines (KG1-a and CCRF-CEM) than the normal cell lines (CD34 + , HRECs and HACAT) [ ], which indicates that Smp24 may be a potential ACP.

Techniques: In Vivo, Staining, Immunohistochemical staining, Control

Journal: Toxins

Article Title: Scorpion Peptide Smp24 Exhibits a Potent Antitumor Effect on Human Lung Cancer Cells by Damaging the Membrane and Cytoskeleton In Vivo and In Vitro

doi: 10.3390/toxins14070438

Figure Lengend Snippet: Acute toxicity analysis of Smp24. ( A – D ) The level of ALT, AST, BUN, Cre in serum of normal mice after 48 h treatment with 0, 5 and 10 mg/kg Smp24. ( E – I ) The weight of heart, liver, spleen, lung and kidney of mice after 48 h treatment with 0, 5 and 10 mg/kg Smp24. ns: no significance.

Article Snippet: The peptide shows stronger cytotoxicity against leukemic tumor cell lines (KG1-a and CCRF-CEM) than the normal cell lines (CD34 + , HRECs and HACAT) [ ], which indicates that Smp24 may be a potential ACP.

Techniques:

Journal: International Journal of Nanomedicine

Article Title: PU.1 Mimic Synthetic Peptides Selectively Bind with GATA-1 and Allow c-Jun PU.1 Binding to Enhance Myelopoiesis

doi: 10.2147/IJN.S303235

Figure Lengend Snippet: ( A ) P1 and P4 (green), P2 and P5 (red), and P3 and P6 (blue) uptake show its permeation in MO7e, KG1a, K562, and HL60 cells as compared to autofluorescence of cells (black). ( B ) Fluorescence images of MO7e cells showing P1, P2, P3, P4, P5, and P6 peptide uptake at 5µg. Green fluorescence (FITC) is shown by peptides’ intake within the cells followed by nucleus staining with DAPI (blue). ( C ) Confocal microscopy of MO7e cells showing P1, P2, P3, P4, P5, and P6 peptide uptake at 5µg/mL.

Article Snippet: The cell lines, KG1a, K562, and HL60 were purchased from National Centre for Cell Sciences (NCCS), Pune, India.

Techniques: Fluorescence, Staining, Confocal Microscopy

Journal: International Journal of Nanomedicine

Article Title: PU.1 Mimic Synthetic Peptides Selectively Bind with GATA-1 and Allow c-Jun PU.1 Binding to Enhance Myelopoiesis

doi: 10.2147/IJN.S303235

Figure Lengend Snippet: Toxicity assessment of P1, P2, P3, P4, P5 P6 treated ( A ) K562, ( B ) KG1a, ( C ) MO7e, and ( D ) HL60 cells. ( E and F ) shows the viability of NPP1, NPP2, NPP3, NPP4, NPP5, and NPP6 treated HL60 cells at 5µg/mL for 40h. $ p<0.005; *p<0.05; @ p<0.01; NS, not statistically significant.

Article Snippet: The cell lines, KG1a, K562, and HL60 were purchased from National Centre for Cell Sciences (NCCS), Pune, India.

Techniques:

Journal: International Journal of Nanomedicine

Article Title: PU.1 Mimic Synthetic Peptides Selectively Bind with GATA-1 and Allow c-Jun PU.1 Binding to Enhance Myelopoiesis

doi: 10.2147/IJN.S303235

Figure Lengend Snippet: Expression of CD11b, CD33, CD116, and CD114 in ( A ) MO7e ( B ) KG1a ( C ) K562 ( D ) HL60 cells treated with P1, P2, and P3 and found; $$ p<0.001; $ p<0.005; @ p<0.01; *p<0.05; NS, not statistically significant, compared to control. ( E ) expression of these markers was also quantified on HL60 cells treated with P4, P5, and P6, and found statistical significance; $$ p<0.001; $ p<0.005 compared to control. ( F ) The CD33 expression on HL60 cells treated with NPP1, NPP2, NPP3, NPP4, NPP5, and NPP6 at 24h and 48h was observed and found; *p<0.05; NS, not statistically significant. ( G ) CD33 expression on HSCs treated with P4, P5, and P6 at 24h was observed and found; *p<0.05; NS, not statistically significant.

Article Snippet: The cell lines, KG1a, K562, and HL60 were purchased from National Centre for Cell Sciences (NCCS), Pune, India.

Techniques: Expressing, Control

Journal: International Journal of Nanomedicine

Article Title: PU.1 Mimic Synthetic Peptides Selectively Bind with GATA-1 and Allow c-Jun PU.1 Binding to Enhance Myelopoiesis

doi: 10.2147/IJN.S303235

Figure Lengend Snippet: Expression of the TFs, PU.1, GATA-1, c-Jun, and C/EBPα in P1, P2, and P3 treated ( A ) MO7e, ( B ) KG1a, ( C ) K562, and ( D ) HL60 cells. In addition, ( E ) the expression of these TFs was also evaluated in P4, P5, and P6 treated HL60 cells and found statistical significance; $$ p<0.001; $ p<0.005; *p<0.05; @ p<0.01; NS, not statistically significant compared to control. ( F ) shows HSCs colony, and ( G ) counted colonies of HSCs after 13 days. $$ p<0.001; NS, not statistically significant.

Article Snippet: The cell lines, KG1a, K562, and HL60 were purchased from National Centre for Cell Sciences (NCCS), Pune, India.

Techniques: Expressing, Control