Journal: Carbohydrate polymers

Article Title: Chitosan - Rosmarinic acid conjugates with antioxidant, anti-inflammatory and photoprotective properties.

doi: 10.1016/j.carbpol.2021.118619

Figure Lengend Snippet: Fig. 5. Cell viability human epidermal fibroblasts (HFB), human epidermal keratinocytes (HEK) and murine macrophages (RAW 264.7) exposed to CSRA lixiviates (A–C) or and free RA (D–F). The diagrams include the mean, SD (n = 16), and the ANOVA results (*p < 0.05 statistically significant difference between the cells in DMEM (control) and treated cells, and #p < 0.05 between the cells treated with different CSRA conjugates or RA concentrations (brackets)).

Article Snippet: The HEK cell line was cultured in the Keratinocytes Medium Kit from Innoprot (Derio, Bizkaia, Spain) and maintained over permissive conditions in a humidified incubator with 5% CO2.

Techniques: Control

Journal: International Journal of Molecular Sciences

Article Title: Cell-Penetrating Delivery of Nitric Oxide by Biocompatible Dinitrosyl Iron Complex and Its Dermato-Physiological Implications

doi: 10.3390/ijms221810101

Figure Lengend Snippet: Cell viability assay of the ( a ) mouse skin melanoma (B16-F10) and ( b ) human skin keratinocyte cells (HaCaT) cells, respectively, treated with different concentrations of DNIC-2 for 24 h **** p < 0.001 compared to the group without treatment of DNIC-2 . ( c ) Cell viability assay of the reconstructed human epidermis (RhE) model treated with PBS, 5% SDS, and 50 μM of DNIC-2 , respectively. ( d ) Cell viability assay of the reconstructed human cornea-like epithelium model treated with DPBS, methyl acetate, and 50 μM of DNIC-2 , respectively.

Article Snippet: CCD-966Sk human skin fibroblasts and B16-F10 mouse skin melanoma cells were purchased from Bioresource Collection and Research Center, Food Industry Research and Development Institute (Hsinchu, Taiwan), whereas human skin keratinocyte cell line, HaCaT, was purchased from Elabscience Biotechnology Inc. (Elabscience ® EP-CL-0090, Houston, TX, USA).

Techniques: Viability Assay

Journal: Scientific Reports

Article Title: Tissue Harvesting Site and Culture Medium Affect Attachment, Growth, and Phenotype of Ex Vivo Expanded Oral Mucosal Epithelial Cells

doi: 10.1038/s41598-017-00417-z

Figure Lengend Snippet: The percentage of attached explants following 48 hours of culture in four different media was compared using explants from four different rat oral mucosa harvesting sites. Each set of bar chart showing percentage of attached explants in EpiLife, oral keratinocyte medium (OKM), Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM), and Roswell Park Memorial Institute 1640 medium (RPMI). The ability of the explants to attach to the culture wells was independent of both harvesting site and type of media.

Article Snippet: Oral keratinocyte medium, oral keratinocyte growth supplement, and penicillin/streptomycin solution (P/S) were obtained from ScienCell Research Laboratory (Carlsbad, CA).

Techniques: Modification

Journal: Scientific Reports

Article Title: Tissue Harvesting Site and Culture Medium Affect Attachment, Growth, and Phenotype of Ex Vivo Expanded Oral Mucosal Epithelial Cells

doi: 10.1038/s41598-017-00417-z

Figure Lengend Snippet: ( A ) Light microscopy images showing representative outgrowth of epithelial cells from rat oral mucosa explants from lower lip region cultured for six days in EpiLife, oral keratinocyte medium (OKM), Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM), and Roswell Park Memorial Institute 1640 medium (RPMI). Red circles indicate the explant area. Green circles indicate the leading edge of the cellular outgrowth. The area between the red and the green circle represents the outgrowth area. ( B ) Bar chart showing fold outgrowth in EpiLife, OKM, DMEM, and RPMI. * P < 0.05. Fold outgrowth was calculated by dividing the outgrowth area by the explant area. Images are representative of four experiments.

Article Snippet: Oral keratinocyte medium, oral keratinocyte growth supplement, and penicillin/streptomycin solution (P/S) were obtained from ScienCell Research Laboratory (Carlsbad, CA).

Techniques: Light Microscopy, Cell Culture, Modification