Journal: Cell Death Discovery

Article Title: Salinomycin as a death switch: how gastric cancer cells choose their demise

doi: 10.1038/s41420-026-03058-2

Figure Lengend Snippet: H2DCFA was used to evaluate ROS production after Sal treatment (48 h) by flow cytometry. Histogram reports the frequencies of ROS positive cells. Sal significantly promoted ROS production in the two cell lines (AGS and KATO-III) not undergoing apoptotic cell death. Data were obtained from three independent biological replicates. T-test was used to evaluate statistical significance (** p < 0.01, **** p < 0.0001).

Article Snippet: SNU1, NCI-N87, AGS, and KATO-III (RRID: CVCL_0099, CVCL_1603, CVCL_0139 and CVCL_0371) GC cells lines were acquired from ATCC (Manassas, VA, USA).

Techniques: Flow Cytometry

Journal: Cell Death Discovery

Article Title: Salinomycin as a death switch: how gastric cancer cells choose their demise

doi: 10.1038/s41420-026-03058-2

Figure Lengend Snippet: A Single cell suspension of Sal- and vehicle-treated cell lines were stained with anti-CD44-FITC and anti-CD133-PE and analyzed by flow cytometry. High levels of both CSC markers were measured in NCI-N87 and KATO-III cells with the latter showing the highest expression. These cell lines, after 48 h of Sal treatment, showed a marked reduction of CD44 + and CD133 + cell populations. Representative histogram overlays of Sal vs vehicle stemness markers positive cell populations. One-sample t -test was used to assess significance, using data from three independent biological replicates. B After 48 h of treatment, cells were harvested and seeded and cultured for 14 days. Morphology and size of spheroids were recorded at 3, 7, 10, and 14 days. The plots summarize radius of Sal-treated cells as compared with vehicle controls from three replicates of two independent experiments. Images were acquired at 10× and 4× for NCI-N87 and KATO-III, respectively. A marked reduction of spheroids size was observed for NCI-N87 cells, while no spheroids formation was observed for KATO-III. C Untreated cells were seeded and treated on day 7 after spheroid formation. Their morphology and size were assessed after 48 h of treatment. Plots report the ratio between spheroids radius before and after treatment from three independent experiments. Sal-treated spheroids were significantly smaller in size as compared with vehicle for both cell lines. D Cells were treated for 48 h and then harvested and seeded for colony-forming assay. Colonies were observed after 14 days of culture, few colonies formed in Sal-treated NCI-N87 cells, and no colonies were found for KATO-III cells. Three independent experiments were performed. T -test was employed to estimate significance (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).

Article Snippet: SNU1, NCI-N87, AGS, and KATO-III (RRID: CVCL_0099, CVCL_1603, CVCL_0139 and CVCL_0371) GC cells lines were acquired from ATCC (Manassas, VA, USA).

Techniques: Single Cell, Suspension, Staining, Flow Cytometry, Expressing, Cell Culture

Journal: Scientific Reports

Article Title: Silver Nanoscale Hexagonal Column Chips for Detecting Cell-free DNA and Circulating Nucleosomes in Cancer Patients

doi: 10.1038/srep10455

Figure Lengend Snippet: Structure of the surface of silver NHC chips with cultured cell samples by using a laser microscope. These are typical images using KATO-III. a, In ×100 view, many small and whitish nodules were recognized on the chip surface after adding a sample of chemically lysed cultured tumor cells. No nodules were observed after adding samples of extracted DNA ( d ) or RNA ( g ). After adding the extracted protein sample, small nodules and starch-like structures were observed ( j ). After adding a sample of physically fractured cultured tumor cells, many relatively large and whitish nodules were observed ( m ). In magnified (×3000 or ×1000) and 3D views, the largest nodule on the chip surface in ×100 view resembled a plain and hillocks (chemically lysed cultured tumor cells and extracted protein samples, b , c , k , l ) or a hill (physically fractured cultured tumor cells, n , o ). No on-chip structure was observed after adding samples of extracted DNA ( e , f ) or RNA ( h , i ).

Article Snippet: Kato III, MKN45, CW-2, and PK45-P were provided by the RIKEN Bioresource Center through the National Bio-Resource Project of the MEXT, Japan.

Techniques: Cell Culture, Microscopy, Starch