Journal: Leukemia

Article Title: The splicing factor PTBP1 interacts with RUNX1 and is required for leukemia cell survival

doi: 10.1038/s41375-025-02799-w

Figure Lengend Snippet: A Growth curve of MOLM-13 ( N = 3) and B Kasumi-1 ( N = 3) sh SCR and sh PTBP1 cells. C Representative plots and quantification from flow cytometry analysis of CD15 and CD11b of MOLM-13 ( N = 10) and D Kasumi-1 ( N = 4) sh SCR and sh PTBP1 cells. E Representative plots and quantification of Annexin V+ cells in MOLM-13 sh SCR and sh PTBP1 cells on day 3 ( N = 6) and F day 7 ( N = 6) of doxycycline induction. G Representative plots and quantification of flow cytometry analysis of Annexin V and DAPI on day 21 of doxycycline induction in MOLM-13 ( N = 3) and H Kasumi-1 ( N = 3) sh SCR and sh PTBP1 cells. % = percentage. For each experiment “ N ” refers to separate doxycycline inductions * = p < 0.05, ** = p < 0.01. **** = p < 0.0001.

Article Snippet: Authenticated Kasumi-1, MV-4-11, REH, MOLT-4, and Jurkat cells were obtained from ATCC (Manassa, VA, USA).

Techniques: Flow Cytometry

Journal: Cancer Biomarkers

Article Title: Glutaminase 1 plays critical roles in myelodysplastic syndrome and acute myeloid leukemia cells

doi: 10.3233/CBM-230454

Figure Lengend Snippet: GLS1 gene expression and activity of venetoclax under hypoxic conditions. (A) SKM-1 cells were cultured in RPMI 1640 medium supplemented with 10% FBS with or without hypoxia for 24 h. Gene expressions of GLS1 and GLS2 were evaluated by RT-PCR. ** p< 0.01 was compared to control. ns, not significant. (B) SKM-1 cells were incubated with RPMI 1640 medium with or without glutamine and/or hypoxia condition for 72 h. Cell viability was analyzed by cell counting kit-8. * p< 0.05 was compared to normoxia. ns, not significant. (C) SKM-1 cells were cultured with or without hypoxia for 24 h. 20S proteasome activity was analyzed. ** p< 0.01 was compared to normoxia. (D) MDS and AML cell lines were cultured in RPMI 1640 medium supplemented with 10% FBS with the indicated concentrations of venetoclax for 72 h in normoxia. Cell growth was evaluated using the CellTiter-Glo ™ Luminescent Cell Viability Assay Kit or Cell Counting Kit-8. (E) MDS and AML cell lines were cultured in RPMI 1640 medium supplemented with 10% FBS with the indicated concentrations of venetoclax for 72 h in normoxia. Cell growth was evaluated using the Cytotoxicity LDH Assay Kit. (F) SKM-1 cells were treated with the indicated concentrations of venetoclax for 48 h. Caspase 3/7 activity was analyzed using the Caspase Glo 3/7 Assay Kit. **** p< 0.0001 were compared to normoxia condition. ns, not significant.

Article Snippet: The MDS cell line SKM-1 (monoblastic leukemia following MDS) and the AML cell lines MOLM-14 (acute monocytic leukemia: AML-M5a) and Kasumi-1 (8;21 chromosome translocation) were obtained from the Japan Research Bioresource Cell Bank (Ibaraki, Osaka, Japan).

Techniques: Gene Expression, Activity Assay, Cell Culture, Reverse Transcription Polymerase Chain Reaction, Control, Incubation, Cell Counting, Cell Viability Assay, Lactate Dehydrogenase Assay, Caspase-Glo Assay

Journal: Cancer Biomarkers

Article Title: Glutaminase 1 plays critical roles in myelodysplastic syndrome and acute myeloid leukemia cells

doi: 10.3233/CBM-230454

Figure Lengend Snippet: Activity of GLS1 inhibitors in MDS and AML cells. (A, B) MDS and AML cell lines were cultured with the indicated concentrations of CB839 or IPN60090 for 72 h under hypoxia. Cell growth was evaluated using the CellTiter-Glo ™ Luminescent Cell Viability Assay Kit or Cell Counting Kit-8. (C, D, E, F) MDS and AML cell lines were cultured with the indicated concentrations of CB838 or IPN60090 for 24 h. The total amount of NADPH or NADP + was analyzed by using NADP/NADPH Assay Kit-WST. (G) SKM-1 cells were incubated with the indicated concentration of CB839 or IPN60090 for 72 h. Intracellular ATP levels were determined using the “Cell” ATP Assay Reagent Ver. 2 Kit. *** p< 0.001, **** p< 0.0001 were compared to control. ns, not significant. (H) SKM-1 cells were incubated with the indicated concentration of CB839 for 24 h. The extracellular OCR and the extracellular acidification were analyzed.

Article Snippet: The MDS cell line SKM-1 (monoblastic leukemia following MDS) and the AML cell lines MOLM-14 (acute monocytic leukemia: AML-M5a) and Kasumi-1 (8;21 chromosome translocation) were obtained from the Japan Research Bioresource Cell Bank (Ibaraki, Osaka, Japan).

Techniques: Activity Assay, Cell Culture, Cell Viability Assay, Cell Counting, Incubation, Concentration Assay, ATP Assay, Control