Journal: Antioxidants

Article Title: 3,3′-Diindolylmethane Promotes BDNF and Antioxidant Enzyme Formation via TrkB/Akt Pathway Activation for Neuroprotection against Oxidative Stress-Induced Apoptosis in Hippocampal Neuronal Cells

doi: 10.3390/antiox9010003

Figure Lengend Snippet: Inhibitory effect of K252a or MK-2206 on neuroprotective action of DIM. HT-22 cells were preincubated with or without DIM in combination with K252a or MK-2206 for 30 min before glutamate challenge. After 12 h, cell viability and ROS level were measured as described in Materials and Methods. ( A ), ( B ) Cell viability and ( C ), ( D ) ROS level. Data are the mean ± SD values of quintuple determinations. ** p < 0.01 versus glutamate-treated group; # p < 0.05 and ## p < 0.01 versus DIM with glutamate-treated group.

Article Snippet: DIM, K252a (a TrkB inhibitor), and MK-2206 (a specific Akt inhibitor) were procured from Cayman Chemical Company (Ann Arbor, MI, USA).

Techniques: