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Promega
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CH Instruments
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CEM Corporation
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Promega
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Promega
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DiscoverX corporation
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iCell Bioscience Inc
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Image Search Results
Journal:
Article Title: Rapid Detection of Clonal T-Cell Receptor-? Gene Rearrangements in T-Cell Lymphomas Using the LightCycler-Polymerase Chain Reaction with DNA Melting Curve Analysis
doi:
Figure Lengend Snippet: Optimization of PCR cycles. DNA samples from 100% Jurkat T-cell line (J, top curve), a mixture of 50% Jurkat and 50% tonsil (0.5 J + 0.5 T, middle curve), 100% tonsil (T, bottom curve), and a negative control including all reagents without DNA (H2O) were amplified for 20 (A), 30 (B), 40 (C), 50 (D), and 60 (E) cycles. B: Jurkat started to produce a sharp −dF/dT peak after 30 PCR cycles. D: The −dF/dT peak height ratio of 100% Jurkat/100% tonsil reached 4.1 at 50 cycles (Table 1). Additional cycles did not significantly improve this ratio. Therefore, the diagnosis of a clonal T-cell gene rearrangement sample was based on DNA melting after 50 PCR cycles. x axis = temperature, ° C; y axis = −dF/dT, where F = fluorescence and T = temperature.
Article Snippet: The polyclonal tonsil background had essentially no influence on the Tm values of the clonally rearranged
Techniques: Negative Control, Amplification, Biomarker Discovery, Fluorescence
Journal:
Article Title: Rapid Detection of Clonal T-Cell Receptor-? Gene Rearrangements in T-Cell Lymphomas Using the LightCycler-Polymerase Chain Reaction with DNA Melting Curve Analysis
doi:
Figure Lengend Snippet: Optimization of LightCycler PCR Cycle Number
Article Snippet: The polyclonal tonsil background had essentially no influence on the Tm values of the clonally rearranged
Techniques:
Journal:
Article Title: Rapid Detection of Clonal T-Cell Receptor-? Gene Rearrangements in T-Cell Lymphomas Using the LightCycler-Polymerase Chain Reaction with DNA Melting Curve Analysis
doi:
Figure Lengend Snippet: Coefficient of Variation (CV) of LightCycler System Melting Curve Analysis
Article Snippet: The polyclonal tonsil background had essentially no influence on the Tm values of the clonally rearranged
Techniques:
Journal:
Article Title: Rapid Detection of Clonal T-Cell Receptor-? Gene Rearrangements in T-Cell Lymphomas Using the LightCycler-Polymerase Chain Reaction with DNA Melting Curve Analysis
doi:
Figure Lengend Snippet: Minimal detection of percent clonal T cell. Jurkat T-cell line DNA (100%, 50%, 25%, 12.5%, 6.25%, and 3.125%) was serially diluted into tonsil DNA. After 50 cycles of amplification in the LightCycler system, we could still detect a distinct peak with the expected Tm of Jurkat by DNA melting curve analysis at the 12.5% level with primer set A (A) and at 6.25% level with primer set C (B).
Article Snippet: The polyclonal tonsil background had essentially no influence on the Tm values of the clonally rearranged
Techniques: Amplification