Journal: Journal of Nanobiotechnology

Article Title: Kupffer cells biomimetic nanoparticles alleviate sepsis related liver injury

doi: 10.1186/s12951-025-03860-7

Figure Lengend Snippet: RNA sequencing in liver tissues of SRLI mice. A PCA analysis (n = 5/group); B Differential gene volcano map (n = 5); C Differential gene enrichment map (n = 5); D Top 10 differentially expressed genes hotspot map (n = 5)

Article Snippet: ZIF-8 (#Z282555-1 g) was obtained from Aladdin Holdings Group Co., Ltd. BRCC3 siRNA (abx909271) was purchased from AmyJet Scientific Inc. BRCC3 (#18,215), pro-caspase-1 (#24,232), Cleaved Caspase-1 (#89,332), pro-IL-1β (#12,703), Cleaved-IL-1β (#83,186), GSDMD-N (#10,137), GSDMD-FL (#97,558), CD80 (#54,521), CD86 (#99,821) and β-actin (#2118) antibodies were purchased from Cell Signaling Technology Company.

Techniques: RNA Sequencing

Journal: Journal of Nanobiotechnology

Article Title: Kupffer cells biomimetic nanoparticles alleviate sepsis related liver injury

doi: 10.1186/s12951-025-03860-7

Figure Lengend Snippet: The effects of BRCC3 on NLRP1. A, B : overall view; C : Detail view; ( D ): Variation of root mean square deviation (RMSD) of complex with time in molecular dynamics simulation; E : The combination mode of BRCC3/NLRP1 after MD was plotted to showed the overall combination mode; F In the process of molecular dynamics simulation, the number of hydrogen bonds changes; G-H : amino acids contributed by top-10. (Amber molecular dynamics software renumbered the amino acids of the protein, NLRP1 amino acid sequence number from 1–786, BRCC3 amino acid sequence number from 787–854);(I)There was an interaction between FLAG-BRCC3 and MYC-NLRP1 (n = 3/group); J The Interaction Relationship between FLAG-BRCC3-N, FLAG-BRCC3-C and MYC-NLRP1 (n = 3/group); K The effect of FLAG-BRCC3 on the ubiquitination of MYC-NLRP1; L FLAG-BRCC3 ubiquitination affects the binding of MYC-NLRP1 and ASC (n = 3/group); M–N interaction between BRCC3 and NLRP1 in Kupffer cell LPS induced model (n = 3/group) (Bar = 20 μM); O-P Co-localization of BRCC3 and NLRP1 in LPS induced in vitro and in vivo models (n = 3/group); I-G The effect of BRCC3 on the binding of NLRP1 and ASC (n = 3/group)

Article Snippet: ZIF-8 (#Z282555-1 g) was obtained from Aladdin Holdings Group Co., Ltd. BRCC3 siRNA (abx909271) was purchased from AmyJet Scientific Inc. BRCC3 (#18,215), pro-caspase-1 (#24,232), Cleaved Caspase-1 (#89,332), pro-IL-1β (#12,703), Cleaved-IL-1β (#83,186), GSDMD-N (#10,137), GSDMD-FL (#97,558), CD80 (#54,521), CD86 (#99,821) and β-actin (#2118) antibodies were purchased from Cell Signaling Technology Company.

Techniques: Software, Sequencing, Ubiquitin Proteomics, Binding Assay, In Vitro, In Vivo