Journal: EMBO Reports

Article Title: The L27 domain of MPP7 enhances TAZ-YY1 cooperation to renew muscle stem cells

doi: 10.1038/s44319-024-00305-4

Figure Lengend Snippet: Reagents and tools table

Article Snippet: Jasplakinolide , Tocris Bioscience , Catalog #2792.

Techniques: Recombinant, Modification, Magnetic Beads, Sequencing, Blocking Assay, Plasmid Preparation, Electron Microscopy, Software, Reporter Assay, Imaging, In Situ

Journal: Research

Article Title: The Mechanics of Tumor Cells Dictate Malignancy via Cytoskeleton-Mediated APC/Wnt/β-Catenin Signaling

doi: 10.34133/research.0224

Figure Lengend Snippet: Softening cells promotes self-renewal and tumorigenicity. (A) Phosphorylation of MLC and cell stiffness after knocking down MLCK and mDia1 using 10 nM siRNAs. n > 80 cells. (B and C) Formation of tumor spheroids in sphere formation assay (SFA) and soft agar assay after HCC cells were softened. n = 3 and 20 for soft agar assay and SFA, respectively. (D) Growth of tumor spheroids generated by softened tumor cells in soft fibrin. Representative of 3 independent experiments. (E) Fractions of EpCAM + , CD90 + , and CD133 + HCC cells. n = 4, 5, and 3 for EpCAM, CD90, and CD133, respectively. (F and G) Colony formation and growth in soft agar and soft fibrin after Huh-7 cells were transfected with the siRNAs of MLCK and mDia1 and then treated with 20 nM jasplakinolide (Jas) or 1 nM narciclasine (Narci). n = 3. (H and I) Tumor growth after the softened Huh-7 cells were inoculated subcutaneously (S.C.) into the flank of nude mice. n = 5 for each condition. After the experiment, the tumor tissues were retrieved and weighted. Scale bar, 1 cm. (J) Tumor growth after the softened MHCC97L cells were prelabeled with luciferase and then inoculated into the liver of nude mice. n = 13 for each condition. (K) Tumor incidence of the softened tumor cells in the limiting dilution assay. In total, 100, 1,000, 10,000, and 100,000 of control and softened Huh-7 cells were inoculated subcutaneously into the flank of nude mice. The tumor incidence was measured at week 8. The stem cell frequency and P value (chi-square test) were calculated using the tools at http://bioinf.wehi.edu.au/software/elda/ . Mean ± SEM. Analysis of variance (ANOVA) was used for the statistical analysis together with the post hoc Bonferroni test. * P < 0.05; ** P < 0.01; *** P < 0.001; # P < 0.05; ## P < 0.01; ### P < 0.001. * or #, the comparison between “si-MLCK” or “si-mDia1” and “Control”. ns: no significance.

Article Snippet: To soften tumor cells, HCC cells were pretreated with different doses of Y-27632 (2 and 20 μM; Selleckchem), blebbistatin (6 and 50 μM; Selleckchem), and cytochalasin D (0.1 and 1 μM; Selleckchem) for 48 h. To stiffen them, the cells were pretreated with 20 nM jasplakinolide (Tocris, #2792/100U) or 1 nM narciclasine (MedChemExpress) for 12 h. Nocodazole (1 and 6 μM; Abcam) and withaferin A (WFA; Abcam; 1, 2, and 5 μM) were used to disrupt microtubules and vimentin, respectively.

Techniques: Tube Formation Assay, Soft Agar Assay, Generated, Transfection, Luciferase, Limiting Dilution Assay, Control, Software, Comparison

Journal: eLife

Article Title: Evolutionarily unique mechanistic framework of clathrin-mediated endocytosis in plants

doi: 10.7554/eLife.52067

Figure Lengend Snippet:

Article Snippet: Chemical compound , Jasplakinolide , Santa Cruz Biotechnology , sc-202191 , .

Techniques: Suspension, Cell Culture