jak2 mutation testing Search Results


jak2  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc jak2
EphrinB2 associates with SHP2, <t>JAK2</t> and STAT1, and modulates STAT1 activity. (a,b) HUVEC infected with eB2-shRNA or eB2-5Y express higher levels of p-STAT1 compared to controls; p-STAT3 levels are similar. Flow cytometry (a; % positive cells is noted on each quadrant; representative of 5 experiments), immunoblotting (b);. (c) Nuclear localization of p-STAT1 (red, arrowheads) in HUVEC transduced with eB2-5Y (GFP: green); little p-STAT1 (red) is detected in HUVEC transduced with eB2-WT (GFP: green); representative images. Scale bar: 20μm (d) EphB4-Fc activates endogenous p-EphrinB and reduces p-STAT1 levels; quantitation (average fluorescence intensity/cell). Results reflect the means±SD from 3 experiments; P values from two-tailed Student t -test: N.S., non significant, * P <0.05, ** P <0.01; error bars: ±SD. (e) Serum starvation time-dependently reduces p-EphrinB levels and increases p-STAT1 levels in HUVEC. (f) EphrinB2 associates with SHP2, JAK2 and STAT1 in HUVEC; cell lysates of HUVEC were immunoprecipitated with antibodies to EphrinB2 or control IgG; precipitates and cell lysates were immunoblotted as indicated. (g–i) Quantitative analysis of EphrinB2 association with SHP2, JAK2 and STAT1 in p5 hyaloid vessels from EphrinB2 WT/WT (n=5) and EphrinB2 5Y/5Y (n=7) mice detected by PEA (top panels). Negative control: reagents alone, no cell lysate. SHP2, JAK2 and STAT1 abundance in PEA input samples (bottom). (j) PLA shows that p-EphrinB associates with SHP2 in hyaloid vessels from EphrinB2 WT/WT mice but not from EphrinB2 5Y/5Y mice. Red: EphrinB2+SHP2; blue: DAPI. Dotted line limits amplified areas in lower panels. Scale bars: 500μm (top panels), 100μm (bottom panels) (k) Quantitation of p-EphrinB+SHP2 proximity co-localization in WT hyaloid vessels regions at p5 and p7. Results (mean±SD fluorescence intensity/mm 2 area) are normalized with DAPI. Error bars: ±SD.
Jak2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/jak2+mutation+testing/Jak2+XP+Rabbit+mAb/pmc04377839-161-65-96
Average 96 stars, based on 1 article reviews
jak2 - by Bioz Stars, 2026-09
96/100 stars
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real-time pcr assay oncoreal jak2 v617f  (Ingenetix gmbh)
90
Ingenetix gmbh real-time pcr assay oncoreal jak2 v617f
EphrinB2 associates with SHP2, <t>JAK2</t> and STAT1, and modulates STAT1 activity. (a,b) HUVEC infected with eB2-shRNA or eB2-5Y express higher levels of p-STAT1 compared to controls; p-STAT3 levels are similar. Flow cytometry (a; % positive cells is noted on each quadrant; representative of 5 experiments), immunoblotting (b);. (c) Nuclear localization of p-STAT1 (red, arrowheads) in HUVEC transduced with eB2-5Y (GFP: green); little p-STAT1 (red) is detected in HUVEC transduced with eB2-WT (GFP: green); representative images. Scale bar: 20μm (d) EphB4-Fc activates endogenous p-EphrinB and reduces p-STAT1 levels; quantitation (average fluorescence intensity/cell). Results reflect the means±SD from 3 experiments; P values from two-tailed Student t -test: N.S., non significant, * P <0.05, ** P <0.01; error bars: ±SD. (e) Serum starvation time-dependently reduces p-EphrinB levels and increases p-STAT1 levels in HUVEC. (f) EphrinB2 associates with SHP2, JAK2 and STAT1 in HUVEC; cell lysates of HUVEC were immunoprecipitated with antibodies to EphrinB2 or control IgG; precipitates and cell lysates were immunoblotted as indicated. (g–i) Quantitative analysis of EphrinB2 association with SHP2, JAK2 and STAT1 in p5 hyaloid vessels from EphrinB2 WT/WT (n=5) and EphrinB2 5Y/5Y (n=7) mice detected by PEA (top panels). Negative control: reagents alone, no cell lysate. SHP2, JAK2 and STAT1 abundance in PEA input samples (bottom). (j) PLA shows that p-EphrinB associates with SHP2 in hyaloid vessels from EphrinB2 WT/WT mice but not from EphrinB2 5Y/5Y mice. Red: EphrinB2+SHP2; blue: DAPI. Dotted line limits amplified areas in lower panels. Scale bars: 500μm (top panels), 100μm (bottom panels) (k) Quantitation of p-EphrinB+SHP2 proximity co-localization in WT hyaloid vessels regions at p5 and p7. Results (mean±SD fluorescence intensity/mm 2 area) are normalized with DAPI. Error bars: ±SD.
Real Time Pcr Assay Oncoreal Jak2 V617f, supplied by Ingenetix gmbh, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/jak2+mutation+testing/real+time+pcr+assay+oncoreal+jak2+v617f/pmc05823561-242-11-22
Average 90 stars, based on 1 article reviews
real-time pcr assay oncoreal jak2 v617f - by Bioz Stars, 2026-09
90/100 stars
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janus kinase 2 v617f mutation test  (Mayo Medical Laboratories)
90
Mayo Medical Laboratories janus kinase 2 v617f mutation test
EphrinB2 associates with SHP2, <t>JAK2</t> and STAT1, and modulates STAT1 activity. (a,b) HUVEC infected with eB2-shRNA or eB2-5Y express higher levels of p-STAT1 compared to controls; p-STAT3 levels are similar. Flow cytometry (a; % positive cells is noted on each quadrant; representative of 5 experiments), immunoblotting (b);. (c) Nuclear localization of p-STAT1 (red, arrowheads) in HUVEC transduced with eB2-5Y (GFP: green); little p-STAT1 (red) is detected in HUVEC transduced with eB2-WT (GFP: green); representative images. Scale bar: 20μm (d) EphB4-Fc activates endogenous p-EphrinB and reduces p-STAT1 levels; quantitation (average fluorescence intensity/cell). Results reflect the means±SD from 3 experiments; P values from two-tailed Student t -test: N.S., non significant, * P <0.05, ** P <0.01; error bars: ±SD. (e) Serum starvation time-dependently reduces p-EphrinB levels and increases p-STAT1 levels in HUVEC. (f) EphrinB2 associates with SHP2, JAK2 and STAT1 in HUVEC; cell lysates of HUVEC were immunoprecipitated with antibodies to EphrinB2 or control IgG; precipitates and cell lysates were immunoblotted as indicated. (g–i) Quantitative analysis of EphrinB2 association with SHP2, JAK2 and STAT1 in p5 hyaloid vessels from EphrinB2 WT/WT (n=5) and EphrinB2 5Y/5Y (n=7) mice detected by PEA (top panels). Negative control: reagents alone, no cell lysate. SHP2, JAK2 and STAT1 abundance in PEA input samples (bottom). (j) PLA shows that p-EphrinB associates with SHP2 in hyaloid vessels from EphrinB2 WT/WT mice but not from EphrinB2 5Y/5Y mice. Red: EphrinB2+SHP2; blue: DAPI. Dotted line limits amplified areas in lower panels. Scale bars: 500μm (top panels), 100μm (bottom panels) (k) Quantitation of p-EphrinB+SHP2 proximity co-localization in WT hyaloid vessels regions at p5 and p7. Results (mean±SD fluorescence intensity/mm 2 area) are normalized with DAPI. Error bars: ±SD.
Janus Kinase 2 V617f Mutation Test, supplied by Mayo Medical Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/jak2+mutation+testing/janus+kinase+2+v617f+mutation+test/pmc05399504-46-7-14
Average 90 stars, based on 1 article reviews
janus kinase 2 v617f mutation test - by Bioz Stars, 2026-09
90/100 stars
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test for the v617f jak2 mutation  (Warnex Inc)
90
Warnex Inc test for the v617f jak2 mutation
EphrinB2 associates with SHP2, <t>JAK2</t> and STAT1, and modulates STAT1 activity. (a,b) HUVEC infected with eB2-shRNA or eB2-5Y express higher levels of p-STAT1 compared to controls; p-STAT3 levels are similar. Flow cytometry (a; % positive cells is noted on each quadrant; representative of 5 experiments), immunoblotting (b);. (c) Nuclear localization of p-STAT1 (red, arrowheads) in HUVEC transduced with eB2-5Y (GFP: green); little p-STAT1 (red) is detected in HUVEC transduced with eB2-WT (GFP: green); representative images. Scale bar: 20μm (d) EphB4-Fc activates endogenous p-EphrinB and reduces p-STAT1 levels; quantitation (average fluorescence intensity/cell). Results reflect the means±SD from 3 experiments; P values from two-tailed Student t -test: N.S., non significant, * P <0.05, ** P <0.01; error bars: ±SD. (e) Serum starvation time-dependently reduces p-EphrinB levels and increases p-STAT1 levels in HUVEC. (f) EphrinB2 associates with SHP2, JAK2 and STAT1 in HUVEC; cell lysates of HUVEC were immunoprecipitated with antibodies to EphrinB2 or control IgG; precipitates and cell lysates were immunoblotted as indicated. (g–i) Quantitative analysis of EphrinB2 association with SHP2, JAK2 and STAT1 in p5 hyaloid vessels from EphrinB2 WT/WT (n=5) and EphrinB2 5Y/5Y (n=7) mice detected by PEA (top panels). Negative control: reagents alone, no cell lysate. SHP2, JAK2 and STAT1 abundance in PEA input samples (bottom). (j) PLA shows that p-EphrinB associates with SHP2 in hyaloid vessels from EphrinB2 WT/WT mice but not from EphrinB2 5Y/5Y mice. Red: EphrinB2+SHP2; blue: DAPI. Dotted line limits amplified areas in lower panels. Scale bars: 500μm (top panels), 100μm (bottom panels) (k) Quantitation of p-EphrinB+SHP2 proximity co-localization in WT hyaloid vessels regions at p5 and p7. Results (mean±SD fluorescence intensity/mm 2 area) are normalized with DAPI. Error bars: ±SD.
Test For The V617f Jak2 Mutation, supplied by Warnex Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/jak2+mutation+testing/jak2+gene/pmc05682567-111-32-23
Average 90 stars, based on 1 article reviews
test for the v617f jak2 mutation - by Bioz Stars, 2026-09
90/100 stars
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chi-squared test  (CH Instruments)
90
CH Instruments chi-squared test
EphrinB2 associates with SHP2, <t>JAK2</t> and STAT1, and modulates STAT1 activity. (a,b) HUVEC infected with eB2-shRNA or eB2-5Y express higher levels of p-STAT1 compared to controls; p-STAT3 levels are similar. Flow cytometry (a; % positive cells is noted on each quadrant; representative of 5 experiments), immunoblotting (b);. (c) Nuclear localization of p-STAT1 (red, arrowheads) in HUVEC transduced with eB2-5Y (GFP: green); little p-STAT1 (red) is detected in HUVEC transduced with eB2-WT (GFP: green); representative images. Scale bar: 20μm (d) EphB4-Fc activates endogenous p-EphrinB and reduces p-STAT1 levels; quantitation (average fluorescence intensity/cell). Results reflect the means±SD from 3 experiments; P values from two-tailed Student t -test: N.S., non significant, * P <0.05, ** P <0.01; error bars: ±SD. (e) Serum starvation time-dependently reduces p-EphrinB levels and increases p-STAT1 levels in HUVEC. (f) EphrinB2 associates with SHP2, JAK2 and STAT1 in HUVEC; cell lysates of HUVEC were immunoprecipitated with antibodies to EphrinB2 or control IgG; precipitates and cell lysates were immunoblotted as indicated. (g–i) Quantitative analysis of EphrinB2 association with SHP2, JAK2 and STAT1 in p5 hyaloid vessels from EphrinB2 WT/WT (n=5) and EphrinB2 5Y/5Y (n=7) mice detected by PEA (top panels). Negative control: reagents alone, no cell lysate. SHP2, JAK2 and STAT1 abundance in PEA input samples (bottom). (j) PLA shows that p-EphrinB associates with SHP2 in hyaloid vessels from EphrinB2 WT/WT mice but not from EphrinB2 5Y/5Y mice. Red: EphrinB2+SHP2; blue: DAPI. Dotted line limits amplified areas in lower panels. Scale bars: 500μm (top panels), 100μm (bottom panels) (k) Quantitation of p-EphrinB+SHP2 proximity co-localization in WT hyaloid vessels regions at p5 and p7. Results (mean±SD fluorescence intensity/mm 2 area) are normalized with DAPI. Error bars: ±SD.
Chi Squared Test, supplied by CH Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/jak2+mutation+testing/chi+square+test/pm24858412-69-17-17
Average 90 stars, based on 1 article reviews
chi-squared test - by Bioz Stars, 2026-09
90/100 stars
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jak2 v617f  (Quest Diagnostics)
86
Quest Diagnostics jak2 v617f
EphrinB2 associates with SHP2, <t>JAK2</t> and STAT1, and modulates STAT1 activity. (a,b) HUVEC infected with eB2-shRNA or eB2-5Y express higher levels of p-STAT1 compared to controls; p-STAT3 levels are similar. Flow cytometry (a; % positive cells is noted on each quadrant; representative of 5 experiments), immunoblotting (b);. (c) Nuclear localization of p-STAT1 (red, arrowheads) in HUVEC transduced with eB2-5Y (GFP: green); little p-STAT1 (red) is detected in HUVEC transduced with eB2-WT (GFP: green); representative images. Scale bar: 20μm (d) EphB4-Fc activates endogenous p-EphrinB and reduces p-STAT1 levels; quantitation (average fluorescence intensity/cell). Results reflect the means±SD from 3 experiments; P values from two-tailed Student t -test: N.S., non significant, * P <0.05, ** P <0.01; error bars: ±SD. (e) Serum starvation time-dependently reduces p-EphrinB levels and increases p-STAT1 levels in HUVEC. (f) EphrinB2 associates with SHP2, JAK2 and STAT1 in HUVEC; cell lysates of HUVEC were immunoprecipitated with antibodies to EphrinB2 or control IgG; precipitates and cell lysates were immunoblotted as indicated. (g–i) Quantitative analysis of EphrinB2 association with SHP2, JAK2 and STAT1 in p5 hyaloid vessels from EphrinB2 WT/WT (n=5) and EphrinB2 5Y/5Y (n=7) mice detected by PEA (top panels). Negative control: reagents alone, no cell lysate. SHP2, JAK2 and STAT1 abundance in PEA input samples (bottom). (j) PLA shows that p-EphrinB associates with SHP2 in hyaloid vessels from EphrinB2 WT/WT mice but not from EphrinB2 5Y/5Y mice. Red: EphrinB2+SHP2; blue: DAPI. Dotted line limits amplified areas in lower panels. Scale bars: 500μm (top panels), 100μm (bottom panels) (k) Quantitation of p-EphrinB+SHP2 proximity co-localization in WT hyaloid vessels regions at p5 and p7. Results (mean±SD fluorescence intensity/mm 2 area) are normalized with DAPI. Error bars: ±SD.
Jak2 V617f, supplied by Quest Diagnostics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/jak2+mutation+testing/jak2+v617f/us12378609-388-28-21
Average 86 stars, based on 1 article reviews
jak2 v617f - by Bioz Stars, 2026-09
86/100 stars
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hel cells  (DSMZ)
94
DSMZ hel cells
EphrinB2 associates with SHP2, <t>JAK2</t> and STAT1, and modulates STAT1 activity. (a,b) HUVEC infected with eB2-shRNA or eB2-5Y express higher levels of p-STAT1 compared to controls; p-STAT3 levels are similar. Flow cytometry (a; % positive cells is noted on each quadrant; representative of 5 experiments), immunoblotting (b);. (c) Nuclear localization of p-STAT1 (red, arrowheads) in HUVEC transduced with eB2-5Y (GFP: green); little p-STAT1 (red) is detected in HUVEC transduced with eB2-WT (GFP: green); representative images. Scale bar: 20μm (d) EphB4-Fc activates endogenous p-EphrinB and reduces p-STAT1 levels; quantitation (average fluorescence intensity/cell). Results reflect the means±SD from 3 experiments; P values from two-tailed Student t -test: N.S., non significant, * P <0.05, ** P <0.01; error bars: ±SD. (e) Serum starvation time-dependently reduces p-EphrinB levels and increases p-STAT1 levels in HUVEC. (f) EphrinB2 associates with SHP2, JAK2 and STAT1 in HUVEC; cell lysates of HUVEC were immunoprecipitated with antibodies to EphrinB2 or control IgG; precipitates and cell lysates were immunoblotted as indicated. (g–i) Quantitative analysis of EphrinB2 association with SHP2, JAK2 and STAT1 in p5 hyaloid vessels from EphrinB2 WT/WT (n=5) and EphrinB2 5Y/5Y (n=7) mice detected by PEA (top panels). Negative control: reagents alone, no cell lysate. SHP2, JAK2 and STAT1 abundance in PEA input samples (bottom). (j) PLA shows that p-EphrinB associates with SHP2 in hyaloid vessels from EphrinB2 WT/WT mice but not from EphrinB2 5Y/5Y mice. Red: EphrinB2+SHP2; blue: DAPI. Dotted line limits amplified areas in lower panels. Scale bars: 500μm (top panels), 100μm (bottom panels) (k) Quantitation of p-EphrinB+SHP2 proximity co-localization in WT hyaloid vessels regions at p5 and p7. Results (mean±SD fluorescence intensity/mm 2 area) are normalized with DAPI. Error bars: ±SD.
Hel Cells, supplied by DSMZ, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/jak2+mutation+testing/HEL/pm24252366-113-0-6
Average 94 stars, based on 1 article reviews
hel cells - by Bioz Stars, 2026-09
94/100 stars
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rpmi 1640 medium  (DSMZ)
96
DSMZ rpmi 1640 medium
EphrinB2 associates with SHP2, <t>JAK2</t> and STAT1, and modulates STAT1 activity. (a,b) HUVEC infected with eB2-shRNA or eB2-5Y express higher levels of p-STAT1 compared to controls; p-STAT3 levels are similar. Flow cytometry (a; % positive cells is noted on each quadrant; representative of 5 experiments), immunoblotting (b);. (c) Nuclear localization of p-STAT1 (red, arrowheads) in HUVEC transduced with eB2-5Y (GFP: green); little p-STAT1 (red) is detected in HUVEC transduced with eB2-WT (GFP: green); representative images. Scale bar: 20μm (d) EphB4-Fc activates endogenous p-EphrinB and reduces p-STAT1 levels; quantitation (average fluorescence intensity/cell). Results reflect the means±SD from 3 experiments; P values from two-tailed Student t -test: N.S., non significant, * P <0.05, ** P <0.01; error bars: ±SD. (e) Serum starvation time-dependently reduces p-EphrinB levels and increases p-STAT1 levels in HUVEC. (f) EphrinB2 associates with SHP2, JAK2 and STAT1 in HUVEC; cell lysates of HUVEC were immunoprecipitated with antibodies to EphrinB2 or control IgG; precipitates and cell lysates were immunoblotted as indicated. (g–i) Quantitative analysis of EphrinB2 association with SHP2, JAK2 and STAT1 in p5 hyaloid vessels from EphrinB2 WT/WT (n=5) and EphrinB2 5Y/5Y (n=7) mice detected by PEA (top panels). Negative control: reagents alone, no cell lysate. SHP2, JAK2 and STAT1 abundance in PEA input samples (bottom). (j) PLA shows that p-EphrinB associates with SHP2 in hyaloid vessels from EphrinB2 WT/WT mice but not from EphrinB2 5Y/5Y mice. Red: EphrinB2+SHP2; blue: DAPI. Dotted line limits amplified areas in lower panels. Scale bars: 500μm (top panels), 100μm (bottom panels) (k) Quantitation of p-EphrinB+SHP2 proximity co-localization in WT hyaloid vessels regions at p5 and p7. Results (mean±SD fluorescence intensity/mm 2 area) are normalized with DAPI. Error bars: ±SD.
Rpmi 1640 Medium, supplied by DSMZ, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/jak2+mutation+testing/RPMI-8226/pmc04225507-134-32-6
Average 96 stars, based on 1 article reviews
rpmi 1640 medium - by Bioz Stars, 2026-09
96/100 stars
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powerplex ® 16 hs system  (Promega)
90
Promega powerplex ® 16 hs system
EphrinB2 associates with SHP2, <t>JAK2</t> and STAT1, and modulates STAT1 activity. (a,b) HUVEC infected with eB2-shRNA or eB2-5Y express higher levels of p-STAT1 compared to controls; p-STAT3 levels are similar. Flow cytometry (a; % positive cells is noted on each quadrant; representative of 5 experiments), immunoblotting (b);. (c) Nuclear localization of p-STAT1 (red, arrowheads) in HUVEC transduced with eB2-5Y (GFP: green); little p-STAT1 (red) is detected in HUVEC transduced with eB2-WT (GFP: green); representative images. Scale bar: 20μm (d) EphB4-Fc activates endogenous p-EphrinB and reduces p-STAT1 levels; quantitation (average fluorescence intensity/cell). Results reflect the means±SD from 3 experiments; P values from two-tailed Student t -test: N.S., non significant, * P <0.05, ** P <0.01; error bars: ±SD. (e) Serum starvation time-dependently reduces p-EphrinB levels and increases p-STAT1 levels in HUVEC. (f) EphrinB2 associates with SHP2, JAK2 and STAT1 in HUVEC; cell lysates of HUVEC were immunoprecipitated with antibodies to EphrinB2 or control IgG; precipitates and cell lysates were immunoblotted as indicated. (g–i) Quantitative analysis of EphrinB2 association with SHP2, JAK2 and STAT1 in p5 hyaloid vessels from EphrinB2 WT/WT (n=5) and EphrinB2 5Y/5Y (n=7) mice detected by PEA (top panels). Negative control: reagents alone, no cell lysate. SHP2, JAK2 and STAT1 abundance in PEA input samples (bottom). (j) PLA shows that p-EphrinB associates with SHP2 in hyaloid vessels from EphrinB2 WT/WT mice but not from EphrinB2 5Y/5Y mice. Red: EphrinB2+SHP2; blue: DAPI. Dotted line limits amplified areas in lower panels. Scale bars: 500μm (top panels), 100μm (bottom panels) (k) Quantitation of p-EphrinB+SHP2 proximity co-localization in WT hyaloid vessels regions at p5 and p7. Results (mean±SD fluorescence intensity/mm 2 area) are normalized with DAPI. Error bars: ±SD.
Powerplex ® 16 Hs System, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/jak2+mutation+testing/powerplex+16+system/pmc05833553-146-26-31
Average 90 stars, based on 1 article reviews
powerplex ® 16 hs system - by Bioz Stars, 2026-09
90/100 stars
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essential thrombocythemia derived set 2 cells  (DSMZ)
94
DSMZ essential thrombocythemia derived set 2 cells
EphrinB2 associates with SHP2, <t>JAK2</t> and STAT1, and modulates STAT1 activity. (a,b) HUVEC infected with eB2-shRNA or eB2-5Y express higher levels of p-STAT1 compared to controls; p-STAT3 levels are similar. Flow cytometry (a; % positive cells is noted on each quadrant; representative of 5 experiments), immunoblotting (b);. (c) Nuclear localization of p-STAT1 (red, arrowheads) in HUVEC transduced with eB2-5Y (GFP: green); little p-STAT1 (red) is detected in HUVEC transduced with eB2-WT (GFP: green); representative images. Scale bar: 20μm (d) EphB4-Fc activates endogenous p-EphrinB and reduces p-STAT1 levels; quantitation (average fluorescence intensity/cell). Results reflect the means±SD from 3 experiments; P values from two-tailed Student t -test: N.S., non significant, * P <0.05, ** P <0.01; error bars: ±SD. (e) Serum starvation time-dependently reduces p-EphrinB levels and increases p-STAT1 levels in HUVEC. (f) EphrinB2 associates with SHP2, JAK2 and STAT1 in HUVEC; cell lysates of HUVEC were immunoprecipitated with antibodies to EphrinB2 or control IgG; precipitates and cell lysates were immunoblotted as indicated. (g–i) Quantitative analysis of EphrinB2 association with SHP2, JAK2 and STAT1 in p5 hyaloid vessels from EphrinB2 WT/WT (n=5) and EphrinB2 5Y/5Y (n=7) mice detected by PEA (top panels). Negative control: reagents alone, no cell lysate. SHP2, JAK2 and STAT1 abundance in PEA input samples (bottom). (j) PLA shows that p-EphrinB associates with SHP2 in hyaloid vessels from EphrinB2 WT/WT mice but not from EphrinB2 5Y/5Y mice. Red: EphrinB2+SHP2; blue: DAPI. Dotted line limits amplified areas in lower panels. Scale bars: 500μm (top panels), 100μm (bottom panels) (k) Quantitation of p-EphrinB+SHP2 proximity co-localization in WT hyaloid vessels regions at p5 and p7. Results (mean±SD fluorescence intensity/mm 2 area) are normalized with DAPI. Error bars: ±SD.
Essential Thrombocythemia Derived Set 2 Cells, supplied by DSMZ, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/jak2+mutation+testing/SET-2/pm25931349-30-15-26
Average 94 stars, based on 1 article reviews
essential thrombocythemia derived set 2 cells - by Bioz Stars, 2026-09
94/100 stars
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quasa test kit  (PrimerDesign Inc)
90
PrimerDesign Inc quasa test kit
EphrinB2 associates with SHP2, <t>JAK2</t> and STAT1, and modulates STAT1 activity. (a,b) HUVEC infected with eB2-shRNA or eB2-5Y express higher levels of p-STAT1 compared to controls; p-STAT3 levels are similar. Flow cytometry (a; % positive cells is noted on each quadrant; representative of 5 experiments), immunoblotting (b);. (c) Nuclear localization of p-STAT1 (red, arrowheads) in HUVEC transduced with eB2-5Y (GFP: green); little p-STAT1 (red) is detected in HUVEC transduced with eB2-WT (GFP: green); representative images. Scale bar: 20μm (d) EphB4-Fc activates endogenous p-EphrinB and reduces p-STAT1 levels; quantitation (average fluorescence intensity/cell). Results reflect the means±SD from 3 experiments; P values from two-tailed Student t -test: N.S., non significant, * P <0.05, ** P <0.01; error bars: ±SD. (e) Serum starvation time-dependently reduces p-EphrinB levels and increases p-STAT1 levels in HUVEC. (f) EphrinB2 associates with SHP2, JAK2 and STAT1 in HUVEC; cell lysates of HUVEC were immunoprecipitated with antibodies to EphrinB2 or control IgG; precipitates and cell lysates were immunoblotted as indicated. (g–i) Quantitative analysis of EphrinB2 association with SHP2, JAK2 and STAT1 in p5 hyaloid vessels from EphrinB2 WT/WT (n=5) and EphrinB2 5Y/5Y (n=7) mice detected by PEA (top panels). Negative control: reagents alone, no cell lysate. SHP2, JAK2 and STAT1 abundance in PEA input samples (bottom). (j) PLA shows that p-EphrinB associates with SHP2 in hyaloid vessels from EphrinB2 WT/WT mice but not from EphrinB2 5Y/5Y mice. Red: EphrinB2+SHP2; blue: DAPI. Dotted line limits amplified areas in lower panels. Scale bars: 500μm (top panels), 100μm (bottom panels) (k) Quantitation of p-EphrinB+SHP2 proximity co-localization in WT hyaloid vessels regions at p5 and p7. Results (mean±SD fluorescence intensity/mm 2 area) are normalized with DAPI. Error bars: ±SD.
Quasa Test Kit, supplied by PrimerDesign Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/jak2+mutation+testing/quasa++test+kit/10__1097_slash_01__hs9__0000567284__78521__cc-81-44-43
Average 90 stars, based on 1 article reviews
quasa test kit - by Bioz Stars, 2026-09
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cell titer glo proliferation assay  (Promega)
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Promega cell titer glo proliferation assay
EphrinB2 associates with SHP2, <t>JAK2</t> and STAT1, and modulates STAT1 activity. (a,b) HUVEC infected with eB2-shRNA or eB2-5Y express higher levels of p-STAT1 compared to controls; p-STAT3 levels are similar. Flow cytometry (a; % positive cells is noted on each quadrant; representative of 5 experiments), immunoblotting (b);. (c) Nuclear localization of p-STAT1 (red, arrowheads) in HUVEC transduced with eB2-5Y (GFP: green); little p-STAT1 (red) is detected in HUVEC transduced with eB2-WT (GFP: green); representative images. Scale bar: 20μm (d) EphB4-Fc activates endogenous p-EphrinB and reduces p-STAT1 levels; quantitation (average fluorescence intensity/cell). Results reflect the means±SD from 3 experiments; P values from two-tailed Student t -test: N.S., non significant, * P <0.05, ** P <0.01; error bars: ±SD. (e) Serum starvation time-dependently reduces p-EphrinB levels and increases p-STAT1 levels in HUVEC. (f) EphrinB2 associates with SHP2, JAK2 and STAT1 in HUVEC; cell lysates of HUVEC were immunoprecipitated with antibodies to EphrinB2 or control IgG; precipitates and cell lysates were immunoblotted as indicated. (g–i) Quantitative analysis of EphrinB2 association with SHP2, JAK2 and STAT1 in p5 hyaloid vessels from EphrinB2 WT/WT (n=5) and EphrinB2 5Y/5Y (n=7) mice detected by PEA (top panels). Negative control: reagents alone, no cell lysate. SHP2, JAK2 and STAT1 abundance in PEA input samples (bottom). (j) PLA shows that p-EphrinB associates with SHP2 in hyaloid vessels from EphrinB2 WT/WT mice but not from EphrinB2 5Y/5Y mice. Red: EphrinB2+SHP2; blue: DAPI. Dotted line limits amplified areas in lower panels. Scale bars: 500μm (top panels), 100μm (bottom panels) (k) Quantitation of p-EphrinB+SHP2 proximity co-localization in WT hyaloid vessels regions at p5 and p7. Results (mean±SD fluorescence intensity/mm 2 area) are normalized with DAPI. Error bars: ±SD.
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EphrinB2 associates with SHP2, JAK2 and STAT1, and modulates STAT1 activity. (a,b) HUVEC infected with eB2-shRNA or eB2-5Y express higher levels of p-STAT1 compared to controls; p-STAT3 levels are similar. Flow cytometry (a; % positive cells is noted on each quadrant; representative of 5 experiments), immunoblotting (b);. (c) Nuclear localization of p-STAT1 (red, arrowheads) in HUVEC transduced with eB2-5Y (GFP: green); little p-STAT1 (red) is detected in HUVEC transduced with eB2-WT (GFP: green); representative images. Scale bar: 20μm (d) EphB4-Fc activates endogenous p-EphrinB and reduces p-STAT1 levels; quantitation (average fluorescence intensity/cell). Results reflect the means±SD from 3 experiments; P values from two-tailed Student t -test: N.S., non significant, * P <0.05, ** P <0.01; error bars: ±SD. (e) Serum starvation time-dependently reduces p-EphrinB levels and increases p-STAT1 levels in HUVEC. (f) EphrinB2 associates with SHP2, JAK2 and STAT1 in HUVEC; cell lysates of HUVEC were immunoprecipitated with antibodies to EphrinB2 or control IgG; precipitates and cell lysates were immunoblotted as indicated. (g–i) Quantitative analysis of EphrinB2 association with SHP2, JAK2 and STAT1 in p5 hyaloid vessels from EphrinB2 WT/WT (n=5) and EphrinB2 5Y/5Y (n=7) mice detected by PEA (top panels). Negative control: reagents alone, no cell lysate. SHP2, JAK2 and STAT1 abundance in PEA input samples (bottom). (j) PLA shows that p-EphrinB associates with SHP2 in hyaloid vessels from EphrinB2 WT/WT mice but not from EphrinB2 5Y/5Y mice. Red: EphrinB2+SHP2; blue: DAPI. Dotted line limits amplified areas in lower panels. Scale bars: 500μm (top panels), 100μm (bottom panels) (k) Quantitation of p-EphrinB+SHP2 proximity co-localization in WT hyaloid vessels regions at p5 and p7. Results (mean±SD fluorescence intensity/mm 2 area) are normalized with DAPI. Error bars: ±SD.

Journal: Nature communications

Article Title: EphrinB2 controls vessel pruning through STAT1-JNK3 signaling

doi: 10.1038/ncomms7576

Figure Lengend Snippet: EphrinB2 associates with SHP2, JAK2 and STAT1, and modulates STAT1 activity. (a,b) HUVEC infected with eB2-shRNA or eB2-5Y express higher levels of p-STAT1 compared to controls; p-STAT3 levels are similar. Flow cytometry (a; % positive cells is noted on each quadrant; representative of 5 experiments), immunoblotting (b);. (c) Nuclear localization of p-STAT1 (red, arrowheads) in HUVEC transduced with eB2-5Y (GFP: green); little p-STAT1 (red) is detected in HUVEC transduced with eB2-WT (GFP: green); representative images. Scale bar: 20μm (d) EphB4-Fc activates endogenous p-EphrinB and reduces p-STAT1 levels; quantitation (average fluorescence intensity/cell). Results reflect the means±SD from 3 experiments; P values from two-tailed Student t -test: N.S., non significant, * P <0.05, ** P <0.01; error bars: ±SD. (e) Serum starvation time-dependently reduces p-EphrinB levels and increases p-STAT1 levels in HUVEC. (f) EphrinB2 associates with SHP2, JAK2 and STAT1 in HUVEC; cell lysates of HUVEC were immunoprecipitated with antibodies to EphrinB2 or control IgG; precipitates and cell lysates were immunoblotted as indicated. (g–i) Quantitative analysis of EphrinB2 association with SHP2, JAK2 and STAT1 in p5 hyaloid vessels from EphrinB2 WT/WT (n=5) and EphrinB2 5Y/5Y (n=7) mice detected by PEA (top panels). Negative control: reagents alone, no cell lysate. SHP2, JAK2 and STAT1 abundance in PEA input samples (bottom). (j) PLA shows that p-EphrinB associates with SHP2 in hyaloid vessels from EphrinB2 WT/WT mice but not from EphrinB2 5Y/5Y mice. Red: EphrinB2+SHP2; blue: DAPI. Dotted line limits amplified areas in lower panels. Scale bars: 500μm (top panels), 100μm (bottom panels) (k) Quantitation of p-EphrinB+SHP2 proximity co-localization in WT hyaloid vessels regions at p5 and p7. Results (mean±SD fluorescence intensity/mm 2 area) are normalized with DAPI. Error bars: ±SD.

Article Snippet: The following primary antibodies were used for immunoblotting: rabbit monoclonal or polyclonal IgG antibodies to: SHP2 (no. 3397; 1:1000), p-VEGFR2 (Tyr 1175 ) (no. 2478; 1:1000); VEGFR2 (no. 2479; 1:1000); p-Erk (Thr 202 /Tyr 204 ) (no. 4370; 1:2000); Erk (p44/p42) (no. 9102; 1:1000); p-EphrinB (Tyr 324/ Tyr 329 ) (no. 3481; 1:1000); cleaved PARP (Asp 214 ), (no. 5625; 1:1000); JNK3 (no. 2305; 1:1000); JAK2 (no. 3230; 1:1000), p-JAK2 (Tyr 1007/1008 ) (no. 3776; 1:1000); STAT1 (no. 9175; 1:1000), p-STAT1 (Tyr 701 ) (no. 9167; 1:1000), p-STAT3 (Tyr 705 ) (no. 9145; 1:2000) (all from Cell Signaling Technology); goat anti-SHP2 (Abcam no. Ab110194; 1:250); rabbit anti-JNK3 (Novus Biologicas no. NBP!-19542; 1:2000); goat anti-actin (Santa Cruz Biotechnology no. sc-1616; 1:).

Techniques: Activity Assay, Infection, shRNA, Flow Cytometry, Western Blot, Transduction, Quantitation Assay, Fluorescence, Two Tailed Test, Immunoprecipitation, Control, Negative Control, Amplification

JNK3 is a target of p-STAT1 regulation. (a) HUVEC-endogenous p-STAT1 induced by serum starvation or IFNγ (10 ng/ml) stimulation specifically binds to JNK3 promoter region. Chromatin IP with p-STAT1 antibodies or control IgG; precipitated DNA was measured by qPCR with specific primers for JNK3 promoters. CM: HUVEC complete medium. Results show means (±SD shown as error bars) from 5 replicates. P values from two-tailed Student t-test: * P <0.05, *** P <0.001. (b) WT EphrinB2 represses JAK2+STAT1-driven JNK3 promoter activity; Gaussia luciferase dual-reporter assay; means (±SD shown as error bars) of 5 experiments. P values from two-tailed Student t-test: *** P <0.001. The JNK3 reporter plasmid was co-transfected in HEK293T cells with expression plasmids for JAK2+STAT1; dominant-negative (DN) JAK2+STAT1; JAK2+STAT1+eB2-WT or JAK2+STAT1+eB2-5Y. (c) Schematic representation of experimental results.

Journal: Nature communications

Article Title: EphrinB2 controls vessel pruning through STAT1-JNK3 signaling

doi: 10.1038/ncomms7576

Figure Lengend Snippet: JNK3 is a target of p-STAT1 regulation. (a) HUVEC-endogenous p-STAT1 induced by serum starvation or IFNγ (10 ng/ml) stimulation specifically binds to JNK3 promoter region. Chromatin IP with p-STAT1 antibodies or control IgG; precipitated DNA was measured by qPCR with specific primers for JNK3 promoters. CM: HUVEC complete medium. Results show means (±SD shown as error bars) from 5 replicates. P values from two-tailed Student t-test: * P <0.05, *** P <0.001. (b) WT EphrinB2 represses JAK2+STAT1-driven JNK3 promoter activity; Gaussia luciferase dual-reporter assay; means (±SD shown as error bars) of 5 experiments. P values from two-tailed Student t-test: *** P <0.001. The JNK3 reporter plasmid was co-transfected in HEK293T cells with expression plasmids for JAK2+STAT1; dominant-negative (DN) JAK2+STAT1; JAK2+STAT1+eB2-WT or JAK2+STAT1+eB2-5Y. (c) Schematic representation of experimental results.

Article Snippet: The following primary antibodies were used for immunoblotting: rabbit monoclonal or polyclonal IgG antibodies to: SHP2 (no. 3397; 1:1000), p-VEGFR2 (Tyr 1175 ) (no. 2478; 1:1000); VEGFR2 (no. 2479; 1:1000); p-Erk (Thr 202 /Tyr 204 ) (no. 4370; 1:2000); Erk (p44/p42) (no. 9102; 1:1000); p-EphrinB (Tyr 324/ Tyr 329 ) (no. 3481; 1:1000); cleaved PARP (Asp 214 ), (no. 5625; 1:1000); JNK3 (no. 2305; 1:1000); JAK2 (no. 3230; 1:1000), p-JAK2 (Tyr 1007/1008 ) (no. 3776; 1:1000); STAT1 (no. 9175; 1:1000), p-STAT1 (Tyr 701 ) (no. 9167; 1:1000), p-STAT3 (Tyr 705 ) (no. 9145; 1:2000) (all from Cell Signaling Technology); goat anti-SHP2 (Abcam no. Ab110194; 1:250); rabbit anti-JNK3 (Novus Biologicas no. NBP!-19542; 1:2000); goat anti-actin (Santa Cruz Biotechnology no. sc-1616; 1:).

Techniques: Chromatin Immunoprecipitation, Control, Two Tailed Test, Activity Assay, Luciferase, Reporter Assay, Plasmid Preparation, Transfection, Expressing, Dominant Negative Mutation