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Image Search Results
Journal: Small (Weinheim an Der Bergstrasse, Germany)
Article Title: Nanoporous Submicron Gold Particles Enable Nanoparticle‐Based Localization Optoacoustic Tomography (nanoLOT)
doi: 10.1002/smll.202404904
Figure Lengend Snippet: Biocompatibility and biosafety of PEGylated gold yarn balls (AuYB). A) Schematic representation of the alamarBlue cell metabolism assay steps: the cells are incubated with AuYB40‐PEG for 24 h and then with alamarBlue (aB) for 4 h, followed by readouts at 540 nm excitation and 580 nm emission wavelength. b) Results of alamarBlue cell viability assay conducted on a Chinese hamster ovary (CHO), murine macrophages (J774A.1) and human umbilical vein endothelial cells (HUVEC) cell cultures fed with different concentrations of AuYB40‐PEG. c) Schematic representation of the cell membrane damage test: the cells were first incubated with AuYB40‐PEG, then with aB to highlight the cytoplasm and sequentially with PBS, DAPI, or Triton X‐100 (TX100) + DAPI. d) Confocal microscopy images of the HUVEC cell culture incubated with aB + TX100 + DAPI, PBS + aB + DAPI, AuYB40‐PEG + aB + DAPI. The blue color indicates the DAPI signal and violet color indicates the aB signal. Scalebar − 100 µm. g) Murine blood biochemistry taken on the 11th day post‐injection of AuYB40‐PEG nanoparticles or phosphate‐buffered saline (PBS, control group): ALB – albumin, ALP – alkaline phosphatase, ALT – alanine transaminase, AMY – amylase, TBIL – total bilirubin, BUN – blood urea nitrogen, CA – calcium, PHOS – phosphates, GLU – glutamine, Na+ – sodium, K+ – potassium, TP – total protein, GLOB –globulin). h) Immune cell content of murine blood collected on the 11th days post‐injection of AuYB40 nanoparticles or PBS (control group): WBC – white blood cells in total, Neu – neutrophils, Lym – lymphocytes, Mon – monocytes, Eos – eosinophils.
Article Snippet: Dulbecco's Modified Eagle's Medium (DMEM), alamarBlue cell assay kit, 4′,6‐diamidino‐2‐phenylindole (DAPI), fetal bovine serum (FBS), 6‐well plates and 96‐well plates were purchased from Thermo Fisher Scientific Inc. Chinese hamster ovary (CHO) cells and
Techniques: Incubation, Viability Assay, Membrane, Confocal Microscopy, Cell Culture, Injection, Saline, Control