j774a.1 cells Search Results


98
ATCC j774a.1
J774a.1, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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CLS Cell Lines Service GmbH j774a
Biocompatibility and biosafety of PEGylated gold yarn balls (AuYB). A) Schematic representation of the alamarBlue cell metabolism assay steps: the cells are incubated with AuYB40‐PEG for 24 h and then with alamarBlue (aB) for 4 h, followed by readouts at 540 nm excitation and 580 nm emission wavelength. b) Results of alamarBlue cell viability assay conducted on a Chinese hamster ovary (CHO), murine macrophages <t>(J774A.1)</t> and human umbilical vein endothelial cells (HUVEC) cell cultures fed with different concentrations of AuYB40‐PEG. c) Schematic representation of the cell membrane damage test: the cells were first incubated with AuYB40‐PEG, then with aB to highlight the cytoplasm and sequentially with PBS, DAPI, or Triton X‐100 (TX100) + DAPI. d) Confocal microscopy images of the HUVEC cell culture incubated with aB + TX100 + DAPI, PBS + aB + DAPI, AuYB40‐PEG + aB + DAPI. The blue color indicates the DAPI signal and violet color indicates the aB signal. Scalebar − 100 µm. g) Murine blood biochemistry taken on the 11th day post‐injection of AuYB40‐PEG nanoparticles or phosphate‐buffered saline (PBS, control group): ALB – albumin, ALP – alkaline phosphatase, ALT – alanine transaminase, AMY – amylase, TBIL – total bilirubin, BUN – blood urea nitrogen, CA – calcium, PHOS – phosphates, GLU – glutamine, Na+ – sodium, K+ – potassium, TP – total protein, GLOB –globulin). h) Immune cell content of murine blood collected on the 11th days post‐injection of AuYB40 nanoparticles or PBS (control group): WBC – white blood cells in total, Neu – neutrophils, Lym – lymphocytes, Mon – monocytes, Eos – eosinophils.
J774a, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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JCRB Cell Bank raw264.7 cell line
Biocompatibility and biosafety of PEGylated gold yarn balls (AuYB). A) Schematic representation of the alamarBlue cell metabolism assay steps: the cells are incubated with AuYB40‐PEG for 24 h and then with alamarBlue (aB) for 4 h, followed by readouts at 540 nm excitation and 580 nm emission wavelength. b) Results of alamarBlue cell viability assay conducted on a Chinese hamster ovary (CHO), murine macrophages <t>(J774A.1)</t> and human umbilical vein endothelial cells (HUVEC) cell cultures fed with different concentrations of AuYB40‐PEG. c) Schematic representation of the cell membrane damage test: the cells were first incubated with AuYB40‐PEG, then with aB to highlight the cytoplasm and sequentially with PBS, DAPI, or Triton X‐100 (TX100) + DAPI. d) Confocal microscopy images of the HUVEC cell culture incubated with aB + TX100 + DAPI, PBS + aB + DAPI, AuYB40‐PEG + aB + DAPI. The blue color indicates the DAPI signal and violet color indicates the aB signal. Scalebar − 100 µm. g) Murine blood biochemistry taken on the 11th day post‐injection of AuYB40‐PEG nanoparticles or phosphate‐buffered saline (PBS, control group): ALB – albumin, ALP – alkaline phosphatase, ALT – alanine transaminase, AMY – amylase, TBIL – total bilirubin, BUN – blood urea nitrogen, CA – calcium, PHOS – phosphates, GLU – glutamine, Na+ – sodium, K+ – potassium, TP – total protein, GLOB –globulin). h) Immune cell content of murine blood collected on the 11th days post‐injection of AuYB40 nanoparticles or PBS (control group): WBC – white blood cells in total, Neu – neutrophils, Lym – lymphocytes, Mon – monocytes, Eos – eosinophils.
Raw264.7 Cell Line, supplied by JCRB Cell Bank, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/j774a%2E1+cells/pmc11204705-128-0-20?v=JCRB+Cell+Bank
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raw264.7 cell line - by Bioz Stars, 2026-08
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Verlag GmbH j774a.1 mouse mf-like cells
Biocompatibility and biosafety of PEGylated gold yarn balls (AuYB). A) Schematic representation of the alamarBlue cell metabolism assay steps: the cells are incubated with AuYB40‐PEG for 24 h and then with alamarBlue (aB) for 4 h, followed by readouts at 540 nm excitation and 580 nm emission wavelength. b) Results of alamarBlue cell viability assay conducted on a Chinese hamster ovary (CHO), murine macrophages <t>(J774A.1)</t> and human umbilical vein endothelial cells (HUVEC) cell cultures fed with different concentrations of AuYB40‐PEG. c) Schematic representation of the cell membrane damage test: the cells were first incubated with AuYB40‐PEG, then with aB to highlight the cytoplasm and sequentially with PBS, DAPI, or Triton X‐100 (TX100) + DAPI. d) Confocal microscopy images of the HUVEC cell culture incubated with aB + TX100 + DAPI, PBS + aB + DAPI, AuYB40‐PEG + aB + DAPI. The blue color indicates the DAPI signal and violet color indicates the aB signal. Scalebar − 100 µm. g) Murine blood biochemistry taken on the 11th day post‐injection of AuYB40‐PEG nanoparticles or phosphate‐buffered saline (PBS, control group): ALB – albumin, ALP – alkaline phosphatase, ALT – alanine transaminase, AMY – amylase, TBIL – total bilirubin, BUN – blood urea nitrogen, CA – calcium, PHOS – phosphates, GLU – glutamine, Na+ – sodium, K+ – potassium, TP – total protein, GLOB –globulin). h) Immune cell content of murine blood collected on the 11th days post‐injection of AuYB40 nanoparticles or PBS (control group): WBC – white blood cells in total, Neu – neutrophils, Lym – lymphocytes, Mon – monocytes, Eos – eosinophils.
J774a.1 Mouse Mf Like Cells, supplied by Verlag GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/j774a%2E1+cells/pm19130551-61-11-17?v=Verlag+GmbH
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j774a.1 mouse mf-like cells - by Bioz Stars, 2026-08
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China Center for Type Culture Collection j774a.1 macrophage cell line
Biocompatibility and biosafety of PEGylated gold yarn balls (AuYB). A) Schematic representation of the alamarBlue cell metabolism assay steps: the cells are incubated with AuYB40‐PEG for 24 h and then with alamarBlue (aB) for 4 h, followed by readouts at 540 nm excitation and 580 nm emission wavelength. b) Results of alamarBlue cell viability assay conducted on a Chinese hamster ovary (CHO), murine macrophages <t>(J774A.1)</t> and human umbilical vein endothelial cells (HUVEC) cell cultures fed with different concentrations of AuYB40‐PEG. c) Schematic representation of the cell membrane damage test: the cells were first incubated with AuYB40‐PEG, then with aB to highlight the cytoplasm and sequentially with PBS, DAPI, or Triton X‐100 (TX100) + DAPI. d) Confocal microscopy images of the HUVEC cell culture incubated with aB + TX100 + DAPI, PBS + aB + DAPI, AuYB40‐PEG + aB + DAPI. The blue color indicates the DAPI signal and violet color indicates the aB signal. Scalebar − 100 µm. g) Murine blood biochemistry taken on the 11th day post‐injection of AuYB40‐PEG nanoparticles or phosphate‐buffered saline (PBS, control group): ALB – albumin, ALP – alkaline phosphatase, ALT – alanine transaminase, AMY – amylase, TBIL – total bilirubin, BUN – blood urea nitrogen, CA – calcium, PHOS – phosphates, GLU – glutamine, Na+ – sodium, K+ – potassium, TP – total protein, GLOB –globulin). h) Immune cell content of murine blood collected on the 11th days post‐injection of AuYB40 nanoparticles or PBS (control group): WBC – white blood cells in total, Neu – neutrophils, Lym – lymphocytes, Mon – monocytes, Eos – eosinophils.
J774a.1 Macrophage Cell Line, supplied by China Center for Type Culture Collection, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/j774a%2E1+cells/pmc09654823-94-1-7?v=China+Center+for+Type+Culture+Collection
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j774a.1 macrophage cell line - by Bioz Stars, 2026-08
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National Centre for Cell Science mouse macrophages j774a.1
Biocompatibility and biosafety of PEGylated gold yarn balls (AuYB). A) Schematic representation of the alamarBlue cell metabolism assay steps: the cells are incubated with AuYB40‐PEG for 24 h and then with alamarBlue (aB) for 4 h, followed by readouts at 540 nm excitation and 580 nm emission wavelength. b) Results of alamarBlue cell viability assay conducted on a Chinese hamster ovary (CHO), murine macrophages <t>(J774A.1)</t> and human umbilical vein endothelial cells (HUVEC) cell cultures fed with different concentrations of AuYB40‐PEG. c) Schematic representation of the cell membrane damage test: the cells were first incubated with AuYB40‐PEG, then with aB to highlight the cytoplasm and sequentially with PBS, DAPI, or Triton X‐100 (TX100) + DAPI. d) Confocal microscopy images of the HUVEC cell culture incubated with aB + TX100 + DAPI, PBS + aB + DAPI, AuYB40‐PEG + aB + DAPI. The blue color indicates the DAPI signal and violet color indicates the aB signal. Scalebar − 100 µm. g) Murine blood biochemistry taken on the 11th day post‐injection of AuYB40‐PEG nanoparticles or phosphate‐buffered saline (PBS, control group): ALB – albumin, ALP – alkaline phosphatase, ALT – alanine transaminase, AMY – amylase, TBIL – total bilirubin, BUN – blood urea nitrogen, CA – calcium, PHOS – phosphates, GLU – glutamine, Na+ – sodium, K+ – potassium, TP – total protein, GLOB –globulin). h) Immune cell content of murine blood collected on the 11th days post‐injection of AuYB40 nanoparticles or PBS (control group): WBC – white blood cells in total, Neu – neutrophils, Lym – lymphocytes, Mon – monocytes, Eos – eosinophils.
Mouse Macrophages J774a.1, supplied by National Centre for Cell Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/j774a%2E1+cells/pmc03491627-94-0-7?v=National+Centre+for+Cell+Science
Average 90 stars, based on 1 article reviews
mouse macrophages j774a.1 - by Bioz Stars, 2026-08
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Biochrom j774a.1 cells
Biocompatibility and biosafety of PEGylated gold yarn balls (AuYB). A) Schematic representation of the alamarBlue cell metabolism assay steps: the cells are incubated with AuYB40‐PEG for 24 h and then with alamarBlue (aB) for 4 h, followed by readouts at 540 nm excitation and 580 nm emission wavelength. b) Results of alamarBlue cell viability assay conducted on a Chinese hamster ovary (CHO), murine macrophages <t>(J774A.1)</t> and human umbilical vein endothelial cells (HUVEC) cell cultures fed with different concentrations of AuYB40‐PEG. c) Schematic representation of the cell membrane damage test: the cells were first incubated with AuYB40‐PEG, then with aB to highlight the cytoplasm and sequentially with PBS, DAPI, or Triton X‐100 (TX100) + DAPI. d) Confocal microscopy images of the HUVEC cell culture incubated with aB + TX100 + DAPI, PBS + aB + DAPI, AuYB40‐PEG + aB + DAPI. The blue color indicates the DAPI signal and violet color indicates the aB signal. Scalebar − 100 µm. g) Murine blood biochemistry taken on the 11th day post‐injection of AuYB40‐PEG nanoparticles or phosphate‐buffered saline (PBS, control group): ALB – albumin, ALP – alkaline phosphatase, ALT – alanine transaminase, AMY – amylase, TBIL – total bilirubin, BUN – blood urea nitrogen, CA – calcium, PHOS – phosphates, GLU – glutamine, Na+ – sodium, K+ – potassium, TP – total protein, GLOB –globulin). h) Immune cell content of murine blood collected on the 11th days post‐injection of AuYB40 nanoparticles or PBS (control group): WBC – white blood cells in total, Neu – neutrophils, Lym – lymphocytes, Mon – monocytes, Eos – eosinophils.
J774a.1 Cells, supplied by Biochrom, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/j774a%2E1+cells/pmc05727424-37-1-15?v=Biochrom
Average 90 stars, based on 1 article reviews
j774a.1 cells - by Bioz Stars, 2026-08
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IDEXX j774a.1 cells
Biocompatibility and biosafety of PEGylated gold yarn balls (AuYB). A) Schematic representation of the alamarBlue cell metabolism assay steps: the cells are incubated with AuYB40‐PEG for 24 h and then with alamarBlue (aB) for 4 h, followed by readouts at 540 nm excitation and 580 nm emission wavelength. b) Results of alamarBlue cell viability assay conducted on a Chinese hamster ovary (CHO), murine macrophages <t>(J774A.1)</t> and human umbilical vein endothelial cells (HUVEC) cell cultures fed with different concentrations of AuYB40‐PEG. c) Schematic representation of the cell membrane damage test: the cells were first incubated with AuYB40‐PEG, then with aB to highlight the cytoplasm and sequentially with PBS, DAPI, or Triton X‐100 (TX100) + DAPI. d) Confocal microscopy images of the HUVEC cell culture incubated with aB + TX100 + DAPI, PBS + aB + DAPI, AuYB40‐PEG + aB + DAPI. The blue color indicates the DAPI signal and violet color indicates the aB signal. Scalebar − 100 µm. g) Murine blood biochemistry taken on the 11th day post‐injection of AuYB40‐PEG nanoparticles or phosphate‐buffered saline (PBS, control group): ALB – albumin, ALP – alkaline phosphatase, ALT – alanine transaminase, AMY – amylase, TBIL – total bilirubin, BUN – blood urea nitrogen, CA – calcium, PHOS – phosphates, GLU – glutamine, Na+ – sodium, K+ – potassium, TP – total protein, GLOB –globulin). h) Immune cell content of murine blood collected on the 11th days post‐injection of AuYB40 nanoparticles or PBS (control group): WBC – white blood cells in total, Neu – neutrophils, Lym – lymphocytes, Mon – monocytes, Eos – eosinophils.
J774a.1 Cells, supplied by IDEXX, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/j774a%2E1+cells/pmc08406339-253-1-5?v=IDEXX
Average 90 stars, based on 1 article reviews
j774a.1 cells - by Bioz Stars, 2026-08
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BioResource International Inc mouse macrophage j774a1 cells
Biocompatibility and biosafety of PEGylated gold yarn balls (AuYB). A) Schematic representation of the alamarBlue cell metabolism assay steps: the cells are incubated with AuYB40‐PEG for 24 h and then with alamarBlue (aB) for 4 h, followed by readouts at 540 nm excitation and 580 nm emission wavelength. b) Results of alamarBlue cell viability assay conducted on a Chinese hamster ovary (CHO), murine macrophages <t>(J774A.1)</t> and human umbilical vein endothelial cells (HUVEC) cell cultures fed with different concentrations of AuYB40‐PEG. c) Schematic representation of the cell membrane damage test: the cells were first incubated with AuYB40‐PEG, then with aB to highlight the cytoplasm and sequentially with PBS, DAPI, or Triton X‐100 (TX100) + DAPI. d) Confocal microscopy images of the HUVEC cell culture incubated with aB + TX100 + DAPI, PBS + aB + DAPI, AuYB40‐PEG + aB + DAPI. The blue color indicates the DAPI signal and violet color indicates the aB signal. Scalebar − 100 µm. g) Murine blood biochemistry taken on the 11th day post‐injection of AuYB40‐PEG nanoparticles or phosphate‐buffered saline (PBS, control group): ALB – albumin, ALP – alkaline phosphatase, ALT – alanine transaminase, AMY – amylase, TBIL – total bilirubin, BUN – blood urea nitrogen, CA – calcium, PHOS – phosphates, GLU – glutamine, Na+ – sodium, K+ – potassium, TP – total protein, GLOB –globulin). h) Immune cell content of murine blood collected on the 11th days post‐injection of AuYB40 nanoparticles or PBS (control group): WBC – white blood cells in total, Neu – neutrophils, Lym – lymphocytes, Mon – monocytes, Eos – eosinophils.
Mouse Macrophage J774a1 Cells, supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/j774a%2E1+cells/pmc07642065-115-16-20?v=BioResource+International+Inc
Average 90 stars, based on 1 article reviews
mouse macrophage j774a1 cells - by Bioz Stars, 2026-08
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Genentech inc differentiated primary macrophages or j774a.1 cells
Biocompatibility and biosafety of PEGylated gold yarn balls (AuYB). A) Schematic representation of the alamarBlue cell metabolism assay steps: the cells are incubated with AuYB40‐PEG for 24 h and then with alamarBlue (aB) for 4 h, followed by readouts at 540 nm excitation and 580 nm emission wavelength. b) Results of alamarBlue cell viability assay conducted on a Chinese hamster ovary (CHO), murine macrophages <t>(J774A.1)</t> and human umbilical vein endothelial cells (HUVEC) cell cultures fed with different concentrations of AuYB40‐PEG. c) Schematic representation of the cell membrane damage test: the cells were first incubated with AuYB40‐PEG, then with aB to highlight the cytoplasm and sequentially with PBS, DAPI, or Triton X‐100 (TX100) + DAPI. d) Confocal microscopy images of the HUVEC cell culture incubated with aB + TX100 + DAPI, PBS + aB + DAPI, AuYB40‐PEG + aB + DAPI. The blue color indicates the DAPI signal and violet color indicates the aB signal. Scalebar − 100 µm. g) Murine blood biochemistry taken on the 11th day post‐injection of AuYB40‐PEG nanoparticles or phosphate‐buffered saline (PBS, control group): ALB – albumin, ALP – alkaline phosphatase, ALT – alanine transaminase, AMY – amylase, TBIL – total bilirubin, BUN – blood urea nitrogen, CA – calcium, PHOS – phosphates, GLU – glutamine, Na+ – sodium, K+ – potassium, TP – total protein, GLOB –globulin). h) Immune cell content of murine blood collected on the 11th days post‐injection of AuYB40 nanoparticles or PBS (control group): WBC – white blood cells in total, Neu – neutrophils, Lym – lymphocytes, Mon – monocytes, Eos – eosinophils.
Differentiated Primary Macrophages Or J774a.1 Cells, supplied by Genentech inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/j774a%2E1+cells/pm36881873-81-2-6?v=Genentech+inc
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differentiated primary macrophages or j774a.1 cells - by Bioz Stars, 2026-08
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iCell Bioscience Inc macrophage line j774a.1
Biocompatibility and biosafety of PEGylated gold yarn balls (AuYB). A) Schematic representation of the alamarBlue cell metabolism assay steps: the cells are incubated with AuYB40‐PEG for 24 h and then with alamarBlue (aB) for 4 h, followed by readouts at 540 nm excitation and 580 nm emission wavelength. b) Results of alamarBlue cell viability assay conducted on a Chinese hamster ovary (CHO), murine macrophages <t>(J774A.1)</t> and human umbilical vein endothelial cells (HUVEC) cell cultures fed with different concentrations of AuYB40‐PEG. c) Schematic representation of the cell membrane damage test: the cells were first incubated with AuYB40‐PEG, then with aB to highlight the cytoplasm and sequentially with PBS, DAPI, or Triton X‐100 (TX100) + DAPI. d) Confocal microscopy images of the HUVEC cell culture incubated with aB + TX100 + DAPI, PBS + aB + DAPI, AuYB40‐PEG + aB + DAPI. The blue color indicates the DAPI signal and violet color indicates the aB signal. Scalebar − 100 µm. g) Murine blood biochemistry taken on the 11th day post‐injection of AuYB40‐PEG nanoparticles or phosphate‐buffered saline (PBS, control group): ALB – albumin, ALP – alkaline phosphatase, ALT – alanine transaminase, AMY – amylase, TBIL – total bilirubin, BUN – blood urea nitrogen, CA – calcium, PHOS – phosphates, GLU – glutamine, Na+ – sodium, K+ – potassium, TP – total protein, GLOB –globulin). h) Immune cell content of murine blood collected on the 11th days post‐injection of AuYB40 nanoparticles or PBS (control group): WBC – white blood cells in total, Neu – neutrophils, Lym – lymphocytes, Mon – monocytes, Eos – eosinophils.
Macrophage Line J774a.1, supplied by iCell Bioscience Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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macrophage line j774a.1 - by Bioz Stars, 2026-08
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CC Pro GmbH mouse monocytes-macrophages cell line j774a.1
Biocompatibility and biosafety of PEGylated gold yarn balls (AuYB). A) Schematic representation of the alamarBlue cell metabolism assay steps: the cells are incubated with AuYB40‐PEG for 24 h and then with alamarBlue (aB) for 4 h, followed by readouts at 540 nm excitation and 580 nm emission wavelength. b) Results of alamarBlue cell viability assay conducted on a Chinese hamster ovary (CHO), murine macrophages <t>(J774A.1)</t> and human umbilical vein endothelial cells (HUVEC) cell cultures fed with different concentrations of AuYB40‐PEG. c) Schematic representation of the cell membrane damage test: the cells were first incubated with AuYB40‐PEG, then with aB to highlight the cytoplasm and sequentially with PBS, DAPI, or Triton X‐100 (TX100) + DAPI. d) Confocal microscopy images of the HUVEC cell culture incubated with aB + TX100 + DAPI, PBS + aB + DAPI, AuYB40‐PEG + aB + DAPI. The blue color indicates the DAPI signal and violet color indicates the aB signal. Scalebar − 100 µm. g) Murine blood biochemistry taken on the 11th day post‐injection of AuYB40‐PEG nanoparticles or phosphate‐buffered saline (PBS, control group): ALB – albumin, ALP – alkaline phosphatase, ALT – alanine transaminase, AMY – amylase, TBIL – total bilirubin, BUN – blood urea nitrogen, CA – calcium, PHOS – phosphates, GLU – glutamine, Na+ – sodium, K+ – potassium, TP – total protein, GLOB –globulin). h) Immune cell content of murine blood collected on the 11th days post‐injection of AuYB40 nanoparticles or PBS (control group): WBC – white blood cells in total, Neu – neutrophils, Lym – lymphocytes, Mon – monocytes, Eos – eosinophils.
Mouse Monocytes Macrophages Cell Line J774a.1, supplied by CC Pro GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse monocytes-macrophages cell line j774a.1 - by Bioz Stars, 2026-08
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Image Search Results


Biocompatibility and biosafety of PEGylated gold yarn balls (AuYB). A) Schematic representation of the alamarBlue cell metabolism assay steps: the cells are incubated with AuYB40‐PEG for 24 h and then with alamarBlue (aB) for 4 h, followed by readouts at 540 nm excitation and 580 nm emission wavelength. b) Results of alamarBlue cell viability assay conducted on a Chinese hamster ovary (CHO), murine macrophages (J774A.1) and human umbilical vein endothelial cells (HUVEC) cell cultures fed with different concentrations of AuYB40‐PEG. c) Schematic representation of the cell membrane damage test: the cells were first incubated with AuYB40‐PEG, then with aB to highlight the cytoplasm and sequentially with PBS, DAPI, or Triton X‐100 (TX100) + DAPI. d) Confocal microscopy images of the HUVEC cell culture incubated with aB + TX100 + DAPI, PBS + aB + DAPI, AuYB40‐PEG + aB + DAPI. The blue color indicates the DAPI signal and violet color indicates the aB signal. Scalebar − 100 µm. g) Murine blood biochemistry taken on the 11th day post‐injection of AuYB40‐PEG nanoparticles or phosphate‐buffered saline (PBS, control group): ALB – albumin, ALP – alkaline phosphatase, ALT – alanine transaminase, AMY – amylase, TBIL – total bilirubin, BUN – blood urea nitrogen, CA – calcium, PHOS – phosphates, GLU – glutamine, Na+ – sodium, K+ – potassium, TP – total protein, GLOB –globulin). h) Immune cell content of murine blood collected on the 11th days post‐injection of AuYB40 nanoparticles or PBS (control group): WBC – white blood cells in total, Neu – neutrophils, Lym – lymphocytes, Mon – monocytes, Eos – eosinophils.

Journal: Small (Weinheim an Der Bergstrasse, Germany)

Article Title: Nanoporous Submicron Gold Particles Enable Nanoparticle‐Based Localization Optoacoustic Tomography (nanoLOT)

doi: 10.1002/smll.202404904

Figure Lengend Snippet: Biocompatibility and biosafety of PEGylated gold yarn balls (AuYB). A) Schematic representation of the alamarBlue cell metabolism assay steps: the cells are incubated with AuYB40‐PEG for 24 h and then with alamarBlue (aB) for 4 h, followed by readouts at 540 nm excitation and 580 nm emission wavelength. b) Results of alamarBlue cell viability assay conducted on a Chinese hamster ovary (CHO), murine macrophages (J774A.1) and human umbilical vein endothelial cells (HUVEC) cell cultures fed with different concentrations of AuYB40‐PEG. c) Schematic representation of the cell membrane damage test: the cells were first incubated with AuYB40‐PEG, then with aB to highlight the cytoplasm and sequentially with PBS, DAPI, or Triton X‐100 (TX100) + DAPI. d) Confocal microscopy images of the HUVEC cell culture incubated with aB + TX100 + DAPI, PBS + aB + DAPI, AuYB40‐PEG + aB + DAPI. The blue color indicates the DAPI signal and violet color indicates the aB signal. Scalebar − 100 µm. g) Murine blood biochemistry taken on the 11th day post‐injection of AuYB40‐PEG nanoparticles or phosphate‐buffered saline (PBS, control group): ALB – albumin, ALP – alkaline phosphatase, ALT – alanine transaminase, AMY – amylase, TBIL – total bilirubin, BUN – blood urea nitrogen, CA – calcium, PHOS – phosphates, GLU – glutamine, Na+ – sodium, K+ – potassium, TP – total protein, GLOB –globulin). h) Immune cell content of murine blood collected on the 11th days post‐injection of AuYB40 nanoparticles or PBS (control group): WBC – white blood cells in total, Neu – neutrophils, Lym – lymphocytes, Mon – monocytes, Eos – eosinophils.

Article Snippet: Dulbecco's Modified Eagle's Medium (DMEM), alamarBlue cell assay kit, 4′,6‐diamidino‐2‐phenylindole (DAPI), fetal bovine serum (FBS), 6‐well plates and 96‐well plates were purchased from Thermo Fisher Scientific Inc. Chinese hamster ovary (CHO) cells and J774A.1 murine macrophages were obtained from CLS Cell Lines Service GmbH.

Techniques: Incubation, Viability Assay, Membrane, Confocal Microscopy, Cell Culture, Injection, Saline, Control