Journal: JCI Insight

Article Title: WNT signaling contributes to the extrahepatic bile duct proliferative response to obstruction in mice

doi: 10.1172/jci.insight.181857

Figure Lengend Snippet: ( A ) qPCR from mouse EHBD organoids for the 4 most abundant WNT ligands of the EHBD. ( B ) Images from mouse organoid cultures 1, 2, and 3 passages after being grown in WNT-free media. ( C ) Bulk RNA-Seq analysis of human organoids for WNT ligands. ( D ) Timeline for C59 administration to inhibit WNT secretion by mouse organoids. ( E – G ) Mouse organoid images ( E ), size ( F ), and viability ( G ) measurements after treatment with 10 μM C59. ( H ) Timeline for IWR-1 administration to inhibit β-catenin in mouse organoids. ( I – K ) Mouse organoid images ( I ), size ( J ), and viability ( K ) following 5 μM IWR-1 treatment. ( L ) Timeline for C59 administration in human organoids. ( M – O ) Human organoid images ( M ), size ( N ), viability ( O ) after 10 μM C59 treatment. ( P ) Timeline for IWR-1 treatment in human organoids. ( Q – S ) Human organoid images ( Q ), size ( R ), and viability ( S ) following 5 μM IWR-1 treatment. Size and viability measurements are normalized to the vehicle controls. Scale bar: 500 μm. One-sample t test. * P < 0.05, ** P < 0.01, *** P < 0.001, n = 3–5 biological replicates.

Article Snippet: IWR-1 (MCE, HY-12238) stock solution was made by reconstituting IWR-1 at a concentration of 10 mM in DMSO and storing it at –80°C.

Techniques: RNA Sequencing